1. Effects of calcitonin gene-related peptide on apoptosis of peripheral blood mononuclear cells from patients with systemic lupus erythematosus
- Author
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Jin Y, Xiaoping Chen, Jiang Pf, Qu R, Tang Zh, Liu C, and Wen Gm
- Subjects
Adult ,Male ,medicine.medical_specialty ,Programmed cell death ,Calcitonin Gene-Related Peptide ,Apoptosis ,Dermatology ,Calcitonin gene-related peptide ,Severity of Illness Index ,Peripheral blood mononuclear cell ,Blood cell ,Internal medicine ,Humans ,Lupus Erythematosus, Systemic ,Medicine ,Cells, Cultured ,Lupus erythematosus ,Dose-Response Relationship, Drug ,business.industry ,Interleukin ,medicine.disease ,medicine.anatomical_structure ,Endocrinology ,Calcitonin ,Leukocytes, Mononuclear ,Interleukin-2 ,Female ,business - Abstract
Summary Background. Systemic lupus erythematosus (SLE) is an autoimmune disease with abnormal apoptosis and autoantibody production. Calcitonin gene-related peptide (CGRP) is a neuropeptide produced by the central and peripheral nervous systems and by endocrine cells. It can influence cell death in thymocytes and cultured smooth muscle cells, and inhibits the production of interleukin (IL)-2, which inhibits apoptosis. Aims. To investigate the effects of calcitonin gene-related peptide (CGRP) on apoptosis of peripheral blood mononuclear cells (PBMCs) from patients with SLE. Methods. The percentage of apoptosis of PBMCs from patients with SLE and healthy blood donors were examined using annexin-V/propidine iodide staining 48 h after culturing with CGRP and/or its receptor antagonist CGRP8-37 at various concentrations. IL-2 activity in culture supernatants was determined using the incorporation of 3H-TdR by the IL-2-dependent cell line CTLL and evaluated by ELISA. Results. The percentage of spontaneous apoptosis of PBMCs from patients with SLE was higher than that of healthy blood donors (34.18 ± 2.86 vs. 18.23 ± 0.81, P
- Published
- 2007
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