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A general function model of spatial straight line is established according to its linear parametric equation. And a structured total least squares algorithm for spatial straight line fitting is investigated in this paper, not only taking into account the errors caused by X, Y, Z three directions and the results impacted by constant columns in the coefficient matrix, but also considering the repeating elements of the coefficient matrix get the same corrections in different locations. The example given in this contribution illustrates that the efficiency and feasibility of this algorithm.

Second harmonic generation (SHG) is a second-order nonlinear optical process that has symmetry constraints confining signal to regions lacking a center of symmetry. Using SHG microscopy, a variety of tissue structures have noninvasively been imaged by virtue of intrinsic signal generated by structured proteins such as collagen fibrils in connective tissues or the actomyosin lattice of muscle cells. In biochemistry and structure biology, the high-level structures of DNA and protein macro-molecules are similar in constructing mechanism, although DNAs consist of deoxynucleotides and proteins of amino acid residues. The principal purpose of present work is to detect the SHG signal from different DNA samples by spectral imaging technology based on two-photon excited fluorescence (TPEF) and SHG. These DNA samples include the solution of genomic DNA and extracted nuclei, and cultured living cells. Results show that we can obtain the SHG signal from solution of genomic DNA and extracted nuclei in routine condition, but nothing from cultured cell nuclei. After adding a little of absolute ethanol (less than 5% by volume) in culture medium, the SHG signal is detectable in the interested region of nuclei. The findings suggest that the interaction between ethanol and DNA in living cell gives rise to the shift of molecular conformation, and this shift changes some nonlinear optical properties of DNA molecules.

Photobiomodulation (PBM) has been mainly used in athlete trauma care. In this paper, the possible applications of PBM in athlete medical care to maintain pro-oxidant-antioxidant homeostasis and in athlete trauma care to treat osteoarthritis and delayed onset of muscular soreness (DOMS) have been discussed. In order to maintain pro-oxidant-antioxidant homeostasis, PBM might be used in an intravascular way, in an endonasal way or in a directly irradiated way. DOMS was supposed to have three phases, z-line disruption, proteolysis of damaged proteins and protein synthesis for myofibril remodeling, each of which might have its own optimum dose of PBM.

Photobiomodulation (PBM) is an effect oflow intensity monochromatic light or laser irradiation (LIL) on biological systems. which stimulates or inhibits biological functions but does not result in irreducible damage. It has been observed that PBM can suppress cellular senescence, reverse skin photoageing and improve fibromyalgia. In this paper, the biological information model of photobiomodulation (BIMP) is used to discuss its mechanism. Cellular senescence can result from short, dysfunctional telomeres, oxidative stress, or oncogene expression, and may contribute to aging so that it can be seen as a decline of cellular function in which cAMP plays an important role, which provide a foundation for PBM on senescence since cellular senescence is a reasonable model of senescence and PBM is a cellular rehabilitation in which cAMP also plays an important role according to BIMP. The PBM in reversing skin photoageing and improving fibromyalgia are then discussed in detail.

Although yin-yang is one of the basic models of traditional Chinese medicine (TCM) for TCM objects such as whole body, five zangs or six fus , they are widely used to discuss cellular processes in papers of famous journals such as Cell, Nature, or Science. In this paper, the concept of the degree of difficulty (DD) of a process was introduced to redefine yin and yang and extend the TCM yin-yang model to the DD yin-yang model so that we have the DD yin-yang inter-transformation, the DD yin-yang antagonism, the DD yin-yang interdependence and the DD yin ping yang mi , which and photobiomodulation (PBM) on cells are supported by each other. It was shown that healthy cells are in the DD yin ping yang mi so that there is no PBM, and there is PBM on non-healthy cells until the cells become healthy so that PBM can be called a cellular rehabilitation. The DD yin-yang inter-transformation holds for our biological information model of PBM. The DD yin-yang antagonism and the DD yin-yang interdependence also hold for a series of experimental studies such as the stimulation of DNA synthesis in HeLa cells after simultaneous irradiation with narrow-band red light and a wide-band cold light, or consecutive irradiation with blue and red light.

Laser surgery provides good exposure with clear operating fields and satisfactory preliminary functional results. In contrast to conventional excision, it was found that matrix metalloproteinases and the tissue inhibitors of metalloproteinases -1 mRNA expression is higher, myofibroblasts appeared and disappeared slower in laser excision wounds. It has been suggested that the better anatomical and functional results achieved following laser cordectomy may be explained by the fact that such procedures result in better, more rapid healing processes to recover vocal cord for early glottic tumors and better. In this paper, the role of photobiomodulation in laser surgery will be discussed by the cultured monolayer normal human skin fibroblast model of the photobiomodulation of marginal irradiation of high intensity laser beam, the photobiomodulation related to the irradiated tissue, the biological information model of photobiomodulation and the animal models of laser surgery. Although high intensity laser beam is so intense that it destroys the irradiated cells or tissue, its marginal irradiation intensity is so low that there is photobiomodulation on non-damage cells to modulate the regeneration of partly damaged tissue so that the surgery of laser of different parameters results in different post-surgical recovery. It was concluded that photobiomodulation might play an important role in the long-term effects of laser surgery, which might be used to design laser surgery.

Light-emitting diode (LED), smaller and less expensive, requires less power than laser does, is more and more popular in studying photobiomodulation. Three new LED systems with different intensity have been designed in this paper. The three LED systems consist of a set of lens to expand the beam of light or/and to focus the rays of light, and an array of LEDs set in the sphere connected with Fresnel lens. The intensity of the three LED systems ranges from: 0.002 to 1.465 W/m 2 with the spot diameter 40 mm; 0.358 to 11.229 W/m 2 with the spot diameter 60 mm; 17.626 to 115.371 W/m 2 with the spot diameter 80 mm. Their experimental formulae for calculating intensity were obtained by SPSS.

In this paper, for the first time, we consider theoretically all the potential orientations of dipoles for second harmonic generation (SHG) under crystallized type I collagen fiber. With high numerical aperture ( NA = 14), the effect of dipole orientation angle Φ on the SHG intensity, on the maximum SHG emergence angles (θ max , φ max ) and the action of these dipole sources on the ratio of forward to backward (F/B) SHG have been studied under different cases of fibrils diameter d1 and the QPM order (m, l) in the crystallized collagen fiber, and it is found that the influential patterns of the same Φ on SHG at different fibrils diameter d1 and the QPM order (m, l) are different. The dipoles arrangement is related to the characteristic changes in biological tissue so that our work may provide a helpful theoretical tool for pathological status detecting.