1. Additional file 4 of Comparison and development of a metagenomic next-generation sequencing protocol for combined detection of DNA and RNA pathogens in cerebrospinal fluid
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Jiang, Hanfang, Xing, Zhihao, Liu, Xiaorong, Chai, Qiang, Xin, Zefeng, Zhu, Chunqing, Lin, Ruihong, Deng, Xuwen, Cui, Dong, Gao, HongDan, and Ma, Dongli
- Abstract
Additional file 4: Fig. S1. The microbial reads percentage of EBV samples and EV71 samples. A. The microbial reads percentage of EBV samples in methods Total DNA , Total NA ,Total RNA and WTA. B. The microbial reads percentage of EV71 samples in methods Total NA ,Total RNA and WTA. Fig. S2. NR, RPMratio and Coverage percent of target pathogens. Subfigure A/B/C shows NR/ RPMratio/coverage percentage of E. coli respectively comparing four sample pretreatment/nuclear acid extraction methods including Microbial DNA, Total DNA, Total NA and Total RNA. The red dots represent concentration of 106cfu/ml and the blue dots represent concentration of 5x104 cfu/ml. Subfigure D/E/F shows NR/ RPMratio/coverage percentage of SP comparing four sample pretreatment/nuclear acid extraction methods respectively. The red dots represent concentration of 106cfu/ml and the blue dots represent concentration of 5x104 cfu/ml. Subfigure G/H/I shows NR/RPMratio/coverage percentage of EBV respectively comparing four sample pretreatment/nuclear acid extraction methods including Total DNA, Total NA, Total RNA and WTA. The red dots represent concentration of 5x105 copies/ml and the blue dots represent concentration of 5x103 copies/ml. Subfigure J/K/L shows NR/RPMratio/coverage percentage of EV71 respectively comparing four sample pretreatment/nuclear acid extraction methods respectively. The red dots represent concentration of 5x105 copies/ml and the blue dots represent concentration of 5x103 copies/ml. Fig. S3. Genomic coverage of pathogens in CSF from encephalitis patients. A. Genomic coverage of Echovirus 30 in clinical CSF 0006. B. Genomic coverage of coxsackievirus B5 in clinical CSF 0010. Fig. S4. Genomic coverage of Streptococcus pneumoniae, Streptococcus thermophilus and Mogibacterium diversum in sample Microbial DNA-ILL -1. A. Genomic coverage of Streptococcus pneumoniae. B. Genomic coverage of Streptococcus thermophilus. C. Genomic coverage of Mogibacterium diversum.
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- 2022
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