Search

ObjectiveWe performed a meta-analysis of randomized, double-blind, controlled trials (RCTs) to systematically investigate the therapeutic effects and tolerability of transcranial alternating current stimulation (tACS) for the treatment of patients with major depressive disorder (MDD).MethodsElectronic search of PubMed, PsycINFO, EMBASE, Chinese National Knowledge Infrastructure, Wanfang database, and the Cochrane Library up to 1 April 2022. Double-blind RCTs examining the efficacy and safety of tACS for patients with MDD were included. The primary outcome was the improvement of depressive symptoms following a course of tACS treatment. Data were analyzed using Review Manager Version 5.3 (Cochrane IMS, Oxford, UK). Study quality was assessed using the Cochrane risk of bias and Jadad scale. Publication bias was assessed using a funnel plot and the Egger test.ResultsWe identified 883 articles, of which 4 RCTs with 5 active treatment arms covering 224 participants with MDD on active tACS (n = 117) and sham tACS (n = 107) were eligible for inclusion. Meta-analysis of depressive symptoms at post-tACS found an advantage of active tACS over sham tACS (n = 212, standard mean difference (SMD) = −1.14, 95% confidence interval (CI): −2.23, −0.06; I2 = 90%, P = 0.04). The significant superiority of active tACS over sham tACS in improving depressive symptoms remained in a sensitivity analysis. Active tACS was significantly superior to sham tACS regarding depressive symptoms at the 4 week follow-up (SMD = −1.07, 95% CI: −2.05, −0.08; I2 = 88%, P = 0.03) and study-defined remission [risk ratio (RR) = 2.07, 95% CI: 1.36, 3.14, I2 = 9%, P = 0.0006]. The discontinuation rate due to any reason was similar between the two groups (P > 0.05). All included studies were rated as high quality (Jadad score ≥ 3), with funnel plots of primary outcome not suggestive of publication bias.ConclusiontACS appeared to be modestly effective and safe for improving depressive symptoms in patients with MDD, although further studies are warranted.

ObjectiveWe performed this systemic review to investigate the therapeutic potential and safety of adjunctive accelerated repetitive transcranial magnetic stimulation (aTMS) for older patients with depression.MethodsWe included published randomized clinical trials (RCTs) and observational studies targeting adjunctive aTMS for older patients with depression.ResultsTwo open-label self-controlled studies (n = 29) fulfilled the criteria for inclusion. The included studies reported significant improvements in depressive symptoms from baseline to post-aTMS (all Ps < 0.05). One study reported a dropout rate of 10.5% (2/19). Mild headache was the most common adverse reaction.ConclusionThe currently available evidence from two open-label self-controlled studies indicates that adjunctive aTMS is a safe and effective therapy for older patients with depression.

In recent years, circular RNA (circRNA) has been found to be involved in a variety of cancer processes. More and more attention has been paid to the research of circRNA in lung cancer. This study aims to investigate whether circ_0000517 affected the physiology of non‐small cell lung cancer (NSCLC) and the underlying mechanism. The results demonstrated that circ_0000517 was highly expressed in lung cancer tissues and cells, and overexpression of circ_0000517 was negatively correlated with the prognosis of NSCLC patients. Silencing of circ_0000517 significantly inhibited the proliferation, glycolysis, and glutamine decomposition of NSCLC cells in vitro and repressed the growth of xenografted tumors in vivo. Moreover, knockdown of circ_0000517 attenuated the expression of PCNA, HK2, LDHA, ASCT2, and GLS1. Further study found that circ_0000517 targeted miR‐330‐5p and miR‐330‐5p targeted YY1. In addition, miR‐330‐5p inhibitor reversed inhibition of cancer cell proliferation, glycolysis, and glutamine decomposition induced by si‐circ_0000517. In conclusion, our study revealed that silencing of circ_0000517 improved the progression of NSCLC through regulating miR‐330‐5p/YY1 axis.

To investigate the prognostic significance of dynamic detection of minimal residual disease (MRD) in patients with acute myeloid leukemia (AML) by 8-color flow cytometry.MRD of 282 AML patients who achieved remission after initial therapy was detected by 8-color flow cytometry. MRD threshold for predicting recurrence was determined by receiver operating characteristic (ROC) curve, and time from MRD-positive to clinical recurrence was analyzed. The differences in overall survival (OS) time and relapse-free survival (RFS) time of patients with different MRD-changes were compared, and the related factors of recurrence in patients with MRD-negative were analyzed by univariate and logistic regression analysis.ROC curve determined that the MFC-MRD threshold for predicting the recurrence of AML was 0.105%, and the recurrence rate of MRD-positive patients was significantly higher than that of MRD-negative patients [52.45% (75/143 cases) vs 35.97% (50/139 cases), P=0.005]. The patients in MRD persistent positive group and negative to positive group recurred earlier than those in positive to negative group and negative-positive fluctuation group (P0.005). Survival analysis showed that OS and RFS time of patients with MRD persistent positive were significantly shorter than those of patients with MRD persistent negative, positive to negative, and negative-positive fluctuation (P0.005). There was no significant difference in OS and RFS between MRD negative to positive group and MRD persistent positive group (P0.005), either between MRD persistent negative group and MRD positive to negative group (P0.005). Among 139 MRD-negative patients, 50 recurred. Univariate and logistic regression analysis showed that the risk of recurrence increased with the increase of white blood cells level (95%CI: 1.000-1.013, P=0.045). The risk of recurrence in patients without hematopoietic stem cell transplantation (HSCT) was 9.694 times higher than that in patients who received HSCT (95%CI: 1.720-54.651, P=0.010), and in the high-risk group was 5.848 times higher than that in the low-risk group (95%CI: 1.418-24.121, P=0.015).The prognosis of AML patients with different MRD changes is significantly different. No matter MRD-positive or MRD-negative at the initial remission, dynamic detection of MRD after treatment is more helpful to accurately guide treatment.多参数流式细胞术动态监测急性髓系白血病微小残留病的临床研究.探讨8色流式细胞术动态监测急性髓系白血病（AML）患者微小残留病（MRD）的预后意义.使用8色流式细胞术对282例初治缓解的AML患者进行MRD动态监测，通过受试者工作特征曲线（ROC）确定预测复发的MRD阈值，分析患者从出现MRDROC曲线确定预测复发的MFC-MRD阈值为0.105％，MRD不同MRD变化情况的AML患者预后存在明显差异，无论患者初次缓解时MRD阳性或阴性，治疗后监测MRD动态变化情况更有助于精准指导治疗.

Few studies have investigated factors associated with acute postsurgical pain (APSP) trajectories, and whether the APSP trajectory can predict chronic postsurgical pain (CPSP) remains unclear. We aimed to identify the predictors of APSP trajectories in patients undergoing gastrointestinal surgery. Moreover, we hypothesised that APSP trajectories were independently associated with CPSP. We conducted a prospective cohort study of 282 patients undergoing gastrointestinal surgery to describe APSP trajectories. Psychological questionnaires were administered 1 day before surgery. Meanwhile, demographic characteristics and perioperative data were collected. Average pain intensity during the first 7 days after surgery was assessed by a numeric rating scale (NRS). Persistent pain intensity was evaluated at 3 and 6 months postoperatively by phone call interview. CPSP was defined as pain at the incision site or surrounding areas of surgery with a pain NRS score ≥ 1 at rest. The intercept and slope were calculated by linear regression using the least squares method. The predictors for the APSP trajectory and CPSP were determined using multiple linear regression and multivariate logistic regression, respectively. Body mass index, morphine milligram equivalent (MME) consumption, preoperative chronic pain and anxiety were predictors of the APSP trajectory intercept. Moreover, MME consumption and preoperative anxiety could independently predict the APSP trajectory slope. The incidence of CPSP at 3 and 6 months was 30.58% and 16.42% respectively. APSP trajectory and age were predictors of CPSP 3 months postoperatively, while female sex and preoperative anxiety were predictive factors of CPSP 6 months postoperatively. Preoperative anxiety and postoperative analgesic consumption can predict APSP trajectory. In addition, pain trajectory was associated with CPSP. Clinicians need to stay alert for these predictors and pay close attention to pain resolution.

Objectives: As a new physical therapeutic technique, magnetic seizure therapy (MST) has established efficacy in the treatment of depression with few cognitive side effects, and thus appears to be a potential alternative to electroconvulsive therapy (ECT). The findings of randomized controlled trials (RCTs) examining the efficacy and safety of MST versus ECT for depression are inconsistent. This systematic review of RCTs was designed with the aim of assessing the safety and efficacy of MST versus ECT for patients with depression. Methods: The WanFang, Chinese Journal Net (CNKI), EMBASE, PubMed, Cochrane Library, and PsycINFO databases were systematically searched by three independent investigators, from their inceptions to July 24, 2021. Results: In total, four RCTs (n = 86) were included and analyzed. Meta-analyses of study-defined response (risk ratio (RR) = 1.36; 95% CI = 0.78 to 2.36; p = 0.28; I2 = 0%), study-defined remission (RR = 1.17; 95% CI = 0.61 to 2.23; p = 0.64; I2 = 0%), and the improvement in depressive symptoms (standardized mean difference (SMD) = 0.21; 95% CI = −0.29 to 0.71; p = 0.42; I2 = 0%) did not present significant differences between MST and ECT. Three RCTs evaluated the cognitive effects of MST compared with ECT using different cognitive measuring tools, but with mixed findings. Only two RCTs reported adverse drug reactions (ADRs), but these lacked specific data. Only one RCT reported discontinuation due to any reason. Conclusions: This preliminary study suggests that MST appears to have a similar antidepressant effect as ECT for depression, but mixed findings on adverse cognitive effects were reported.

Objectives Smoking prevalence has significantly increased among Chinese adolescences in the past decades. The aim of our study is to investigate the trends and changing patterns in age of smoking initiation among the Chinese population. Study design Pooled data from the 2006–2015 China Health and Nutrition Survey was used for analysis. A total of 10,032 adults aged ≥18 years who were born between 1950 and 1997 were separated into five birth cohorts (1950–1959, 1960–1969, 1970–1979, 1980–1989 and 1990–1997). Methods Age-specific (10–24 years) smoking initiation rates were calculated by gender, educational level and urbanisation. The multiple logistic models were used for estimates of changes in smoking initiation age. Results The mean age of smoking initiation decreased substantially from 22.0 years (95% confidence interval [CI]: 21.2–22.7) to 17.5 years (95% CI: 16.2–19.1) over five generations. A large decrease was seen in the initiation age group of 15–24 years in the 1980s cohort (15–19 years: odds ratio [OR] = 0.57; 95% CI: 0.19–0.97; 20–24 years: OR = 0.45; 95% CI: 0.18–0.82); a significant decrease was also found in the 1990s cohort (15–19 years: OR = 0.47; 95% CI: 0.11–0.94; 20–24 years old: OR = 0.34; 95% CI: 0.14–0.85). The peak age of smoking initiation changed from 20 years old to 18 years old over the five generations. Conclusions The age of smoking initiation has decreased rapidly in the Chinese population in the past decades. Chinese adolescents are becoming the main target group for the tobacco marketing industry, and national legislations are urgently required.

Background. Quadratus lumborum block (QLB) has been used to reduce postoperative acute pain and opioid consumption. However, the efficacy of QLB on the quality of recovery (QoR) after gastrointestinal surgery has not been established. The aim of this study was to evaluate the ability of QLB to enhance the postoperative QoR in patients undergoing open gastrointestinal surgery. Methods. Eighty-four patients undergoing open gastrointestinal surgery were randomized to receive ultrasound-guided QLB with either 20 ml of 0.375% ropivacaine or saline. The primary outcome was the QoR-15 score at 24 h after surgery. The secondary outcomes were the postoperative pain intensity, opioid consumption, the incidence of nausea, vomiting, and chronic pain. Results. The global QoR-15 score at 24 h postoperatively was significantly higher in the QLB group than in the control group (mean difference: 16.9; 95% CI: 11.9–21.9). Additionally, the QoR-15 scores for five dimensions were significantly higher in the QLB group than in the control group. The cumulative oxycodone consumption was significantly lower in the QLB group during 0–6, 6–24, 0–24, 24–48, and 0–48 h postoperatively than in the control group. At rest or during coughing, the pain verbal rating scale scores were significantly lower at 1, 3, 6, 12, and 24 h after surgery in the QLB group than in the control group. The incidence of postoperative nausea was significantly different between the groups, but postoperative vomiting was not. Conclusion. Single-injection posteromedial QLB with ropivacaine enhanced the QoR at 48 h after surgery and improved analgesia during the early postoperative period in patients undergoing gastrointestinal surgery.

This is the Supplementary Table for the article "Sub-macroscopic Skin Presentation of Acromegaly and Effect of Pituitary Tumor Surgery", showing the correlations between postoperative remission and skin lesion improvement.

Regional anesthesia has been used to reduce acute postsurgical pain and to prevent chronic pain. The best technique, however, remains controversial.The aim of this study was to assess the short- and long-term postoperative analgesic efficacy of ultrasound-guided quadratus lumborum block (QLB) in open gastrointestinal surgery.A randomized, double-blinded, controlled trial.Operating room; postoperative recovery room and ward.One hundred eighteen patients underwent elective gastrointestinal surgery randomly assigned into 2 groups (QLB group or control group). Before anesthetic induction, QLB was performed bilaterally under ultrasound guidance using 20 mL of 0.375% ropivacaine or saline solution at each abdominal wall. The primary outcome was cumulative oxycodone consumption within 24 h after surgery. The secondary outcomes were acute pain intensity, incidence of chronic pain, and incidence of postoperative nausea or vomiting (PONV), dizziness, and pruritus.The cumulative oxycodone consumption was significantly lower in the QLB group during the first 6, 6-24, 24, and 48 h postoperatively when compared to the control group. At rest or during coughing, the numeric rating scale scores were significantly lower at 1, 3, 6, and 12 h postoperatively in the QLB group compared to the control group. There were no significant differences between the 2 groups regarding the incidence of chronic postoperative pain at 3 or 6 months after surgery. Significant differences were found in the incidence of PONV between the two groups, but other complications, such as dizziness and pruritus, did not differ significantly.We did not confirm the QLB effectiveness with sensory level testing after local anesthetic injection. Cumulative oxycodone consumption could have been affected by the patients' use of oxycodone for nonsurgical pain.Ultrasound-guided QLB provided superior short-term analgesia and reduced oxycodone consumption and the incidence of PONV after gastrointestinal surgery. However, the incidence of chronic pain was not significantly affected by this anesthetic technique.

We reviewed the outcomes of 77 episodes of CD19 CAR-T therapy in 67 patients with B cell hematological malignancies from October 2016 to January 2020. Factors related to the grade of cytokine release syndrome (CRS) were explored by multivariate analysis, nonparametric test was conducted to explore the correlation between CRS and response. Kaplan-Meier curves were used to indicate survival profiles, and the correlation between CRS and survival was determined by the log-rank test.The rate of complete remission (CR) was 74.0% (57/77). CRS of any grade occurred in 68 of 77 episodes (grade 1: 32.5%, grade 2: 24.7%, grade 3: 22.1%, grade 4: 6.5%, grade 5: 2.6%). Patients with a history of transplantation had less severe CRS, and dose escalation-based infusion reduced the severity of CRS. Severe CRS was related to a higher CR rate but had no significant impact on event-free survival (EFS), relapse-free survival (RFS), or overall survival (OS).As a common adverse reaction of CAR-T therapy, the severity of CRS can be alleviated by dose escalation infusion, a history of transplantation was correlated with less severe CRS. Severe CRS was related to better response but was unrelated to long-term survival.

IL6 is one of the most elevated cytokines during chimeric antigen receptor (CAR) T cell cytokine release syndrome (CRS), and IL6R blockade by Tocilizumab has successfully relieved the most life-threatening aspects of CRS in patients. In addition, latest studies demonstrated the essential role of IL1 in driving CART induced neurotoxicity in mouse models. Here we present a clinical investigation (ChiCTR2000032124; ChiCTR2000031868) of anti-CD19 and anti-BCMA CART (41BBζ) secreting an anti-IL6 scFv and IL1 receptor antagonist (IL1RA) in treating patients with hematologic malignancy. Our results revealed that IL6 and IL1B were maintained at low levels without significant elevation during CRS, rendering Tocilizumab dispensable. Moreover, treated patients did not show neurotoxicity during CRS and exhibited mild to moderate CRS. Notably, we observed high rate of complete response (CR) and significant CART expansion during treatment. In sum, we conclude that CART-secreting anti-IL6 scFv and IL1RA could self-neutralize IL6 storm and maintain low levels of IL1B during CART therapy to minimize IL6- and IL1-associated cytokine toxicity and neurotoxicity without impairing therapeutic efficacy.

Background. Several predictors have been shown to be independently associated with chronic postsurgical pain for gastrointestinal surgery, but few studies have investigated the factors associated with acute postsurgical pain (APSP). The aim of this study was to identify the predictors of APSP intensity and severity through investigating demographic, psychological, and clinical variables. Methods. We performed a prospective cohort study of 282 patients undergoing gastrointestinal surgery to analyze the predictors of APSP. Psychological questionnaires were assessed 1 day before surgery. Meanwhile, demographic characteristics and perioperative data were collected. The primary outcomes are APSP intensity assessed by numeric rating scale (NRS) and APSP severity defined as a clinically meaningful pain when NRS ≥4. The predictors for APSP intensity and severity were determined using multiple linear regression and multivariate logistic regression, respectively. Results. 112 patients (39.7%) reported a clinically meaningful pain during the first 24 hours postoperatively. Oral morphine milligram equivalent (MME) consumption (β 0.05, 95% CI 0.03–0.07, p < 0.001 ), preoperative anxiety (β 0.12, 95% CI 0.08–0.15, p < 0.001 ), and expected postsurgical pain intensity (β 0.12, 95% CI 0.06–0.18, p < 0.001 ) were positively associated with APSP intensity. Furthermore, MME consumption (OR 1.15, 95% CI 1.10–1.21, p < 0.001 ), preoperative anxiety (OR 1.33, 95% CI 1.21–1.46, p < 0.001 ), and expected postsurgical pain intensity (OR 1.36, 95% CI 1.17–1.57, p < 0.001 ) were independently associated with APSP severity. Conclusion. These results suggested that the predictors for APSP intensity following gastrointestinal surgery included analgesic consumption, preoperative anxiety, and expected postsurgical pain, which were also the risk factors for APSP severity.

To investigate the safety and efficacy of tumor-associated antigen-specific cytotoxic T lympho- cytes (TAA-CTL) in the treatment of multiple myeloma (MM) and non-Hodgkin lymphoma (NHL).Peripheral blood mononuclear cells (PBMNC)of patients were collected. Dendritic cells (DC) were loaded with multiple tumor-associated antigens (TAA) (NY-ESO-1, MAGE-A3, MAGE-A4, WT1, Survivin, PRAME, LMP1 and LMP2A), then co-cultured with PBMNC to induce cytotoxic T lymphocytes (CTL). The phenotypes of cell products were detected, and the disease statuse was evaluated in 7 patients during or after infusion. The changes of TAA-CTL amount in the PBMNC of patients were measured by using IFN-γ ELISpot assay.TAA-CTL products were generated comprising CD3TAA-CTLs preliminarily show its safety and efficacy in MM and NHL patients, however, a larger population sample is needed to explore its clinical application value.树突状细胞介导的肿瘤相关抗原特异性T淋巴细胞治疗多发性骨髓瘤和非霍奇金淋巴瘤的临床研究.初步探究靶向肿瘤相关抗原特异性T淋巴细胞（TAA-CTL）治疗多发性骨髓瘤（MM）和非霍奇金淋巴瘤（NHL）的安全性和有效性.采集患者自身外周血单个核细胞（PBMNC），使用含有多种肿瘤相关抗原（TAA）（NY-ESO-1，MAGE-A3，MAGE-A4，WT1，Survivin，PRAME，LMP2A和LMP1）的混合多肽，负载树突状细胞（DC），刺激共培养细胞毒性T细胞（CTL），测定细胞产物表型，并对7例回输后的患者进行病情评估。同时，利用IFN-γ ELISpot试验检测输注前、后患者外周血中TAA-CTL含量的变化.平均82.98%的TAA-CTL产物为CD3TAA-CTL初步显示了其对MM和NHL患者的安全性和有效性，但还需更大样本量以探讨其临床应用价值.

Purpose Visual impairment assessment plays a significant role in diagnosis and clinical decisions in nonfunctioning pituitary adenoma (NFPA). Retinal microvascular alterations may potentially reflect the visual impairment. The purpose of this study was to evaluate fundus microvascular alterations in patients with NFPA. Methods Comparisons of visual field, retinal structure, and microcirculation were conducted between patients with NFPA and age- and sex-matched healthy controls. Multivariate regression modeling was used to assess the relationship between symptom duration, sellar mass size, and fundus vessel density (VD) of patients. Receiver operating characteristic (ROC) curves were depicted to determine the diagnostic performance of significant parameters to discriminate eyes with NFPA from healthy eyes. Results Forty eyes of patients and 40 eyes of healthy individuals were enrolled. Compared to healthy controls, patients with NFPA had a larger foveal avascular zone area and thinner ganglion cell complex thickness. The VDs of patients presented an obvious decrease in radial peripapillary capillary (RPC) and an increase in the deep capillary plexus segment of the perifoveal area. The peripapillary VDs of patients were significantly related to sellar mass size. The area under the ROC curve of VD in the RPC segment of the temporo-inferior sector was the largest (0.801; 95% confidence interval, 0.70–0.90; P < 0.001). Conclusions The fundus of patients with NFPA demonstrated significant and characteristic microvascular impairments. The VDs were also significantly associated with sellar mass size. Translational Relevance Retinal microvascular alterations detected by optical coherence tomography angiography are characteristic and related to sellar mass size, which may provide information that facilitates the diagnosis of NFPA.

To investigate the effect of chidamide on the killing activity of NK (Natural killer cell, NK) cells targeting K562 cells and its related mechanism.K562 cells were pretreated with chidamide at different concentrations and cocultured with NK cells at different effect-target ratios. The killing effect of chidamide on K562 cells by NK cells, the expression of natural killer group 2 member D (NKG2D) ligands and apoptosis rate of K562 cells were detected by flow cytometry.The killing sensitivity of NK cells to K562 cells could be enhanced by chidamide. The expression of ULBP2 on K562 cell surface could be up-regulate, however, the expression of ULBP1 and MICA/MICB showed no statistically difference as compared with control group. Chidamide showed no obvious cytotoxicity to K562 cells.Chidamide can significantly improve killing efficiency of NK cells on K562 cells, which may be related to the up-regulation of ULBP2 expression.西达本胺对NK细胞体外杀伤K562细胞活性的影响及其相关机制.观察西达本胺对自然杀伤细胞（NK）体外杀伤K562细胞活性的影响并探讨其作用机制.用不同浓度的西达本胺预处理K562细胞，与NK细胞不同效靶比共同孵育，采用流式细胞术检测西达本胺作用于K562细胞后NK细胞对其杀伤作用、K562细胞表面NKG2D相关配体表达量及K562细胞凋亡率的变化.西达本胺可增加K562细胞对NK细胞杀伤的敏感性，同时可上调K562细胞表面ULBP2表达，对ULBP1、MICA/MICB表达无明显影响，西达本胺于K562细胞本身无明显细胞毒性作用.西达本胺可能通过增加ULBP2的表达提高K562细胞对NK细胞杀伤敏感性.

To investigate the killing effect of NK-92MI cells modified by chimeric antigen receptor (CD7-CAR) and specifically targeting CD7 to CD7Three types of hematological malignant tumor cells, including 5 cases of CD7The killing efficiencies of CD7-CAR-modified NK-92MI cells to CD7CD7-CAR-modified NK-92MI cells have significantly improved killing efficiency against CD7靶向CD7的嵌合抗原受体NK-92MI细胞对CD7阳性血液系统恶性肿瘤细胞的杀伤作用.探讨特异性靶向CD7分子的嵌合抗原受体（CD7-CAR）修饰的NK-92MI细胞对CD7阳性血液系统恶性肿瘤细胞的杀伤作用.分别收集5例CD7CD7-CAR修饰的NK-92MI细胞对CD7经CD7-CAR修饰的NK-92MI细胞对CD7

The effects of macronutrient intake on obesity are controversial. This research aims to investigate the associations between macronutrient intake and new-onset overweight/obesity. The relationship between the consumption of carbohydrate and total fat and obesity was assessed by the multivariable Cox model in this 11-year cohort, which included 6612 adults (3291 men and 3321 women) who were free of overweight and obesity at baseline. The dietary intake was recorded using a 24-h recall method for three consecutive days. Moreover, substitution models were developed to distinguish the effects of macronutrient composition alteration from energy intake modification. During 7·5 person years (interquartile range 4·3, 10·8) of follow-up, 1807 participants became overweight or obese. After adjusting for risk factors, the hazard ratio (HR) of overweight/obesity in extreme quintiles of fat was 1·48 (quintile 5 v. quintile 1, 95 % CI 1·16, 1·89; Ptrend = 0·02) in women. Additionally, replacing 5 % of energy from carbohydrate with equivalent energy from fat was associated with an estimated 4·3 % (HR 1·043, 95 % CI 1·007, 1·081) increase in overweight/obesity in women. Moreover, dietary carbohydrate was inversely associated with overweight/obesity (quintile 5 v. quintile 1, HR 0·70, 95 % CI 0·55, 0·89; Ptrend = 0·02) in women. Total fat was related to a higher risk of overweight/obesity, whereas high carbohydrate intake was related to a lower risk of overweight/obesity in women, which was not observed in men.

These two questionnaires were used for acromegaly-associated symptoms and comorbidities in the 2019 China Rare Disease Survey for acromegaly. These materials are published here as the supplementary files of the manuscript entitled "Quality of life and its determinants in patients with treated acromegaly: a cross-sectional nationwide study in China".

This part includes 4 supplemental figures, 4 supplemental tables , and 1 supplementary figure legend. These files are all supplemental materials for the article entitled "The immune profile of pituitary adenomas and a novel immune classification for predicting immunotherapy responsiveness" submitted to the Journal of Clinical Endocrinology and Metabolism.

A wideband bandpass filter (BPF) with an adjustable notched-band and high selectivity is proposed. The proposed BPF consists of a multi-mode resonator (MMR), twoλ/2 resonators, and I/O feed lines with 50 ohm characteristic impedance. The MMR, connected as a whole by a wide stub, is composed of one I-shaped resonator and two open-loop resonators. Tightly coupling is built between MMR andλ/2 resonators. I/O feed lines are directly connected with twoλ/2 resonators, respectively. Due to the use of tapped-line coupling, one transmission zero (TZ) is formed near low-edge of aim passband. High-edge of passband with one attendant TZ can be tuned to desired location by adjusting bottom-side position of used wide stub or bottom-side length of I-shaped resonator in MMR. The top-side length of I-shaped resonator is applied to improve upper stopband performance and shift undesired resonant mode of MMR near high-edge of aim passband to proper frequency point. The notched-band in aim passband is dominated by top-side position of wide stub in MMR. Good agreement is observed between simulated and measured results.

Spinal cord ischemia reperfusion injury (SCIRI) can cause spinal cord dysfunction and even devastating paraplegia. Calcium-sensing receptor (CaSR) and calpain are two calcium related molecules which have been reported to be involved in the ischemia reperfusion injury of cardiomyocytes and the subsequent apoptosis. Here, we studied the expression of CaSR and calpain in spinal cord neurons and tissues, followed by the further investigation of the role of CaSR/calpain axis in the cellular apoptosis process during SCIRI. The results of in vitro and in vivo studies showed that the expression of CaSR and calpain in spinal cord neurons increased during SCIRI. Moreover, the CaSR agonist GdCl3 and antagonist NPS-2390 enhanced or decreased the expression of CaSR and calpain respectively. The expressions of CaSR and calpain were also consistent with the cellular apoptosis in spinal cord. Taken together, CaSR-calpain contributes to the SCIRI apoptosis, and CaSR antagonist might be a helpful drug for alleviating SCIRI.

To investigate the relationship between the expression of lysosomal membrane proteins LAMP1, TPC1 and TPC2 in acute myeloid leukemia (AML) cells and clinical indications of AML and to explore the possible role in the genesis and development of AML and clinical significance.Real-time quantitative PCR was used to detect the mRNA expression of LAMP1, TPC1 and TPC2 in AML cell lines (HL-60, NB4) and 57 patients with acute myeloid leukemia (including 44 initially treated patients and 13 relapsed and refractory patients). The relationship of mRNA expression levels with clinical indicators and post-chemotherapy remission was analyzed.Compared with CD34The mRNA of the three membrane proteins are highly expressed in AML patients, and LAMP1 and TPC1 are risk factors for AML disease progression. High expression of TPC2 is beneficial for chemotherapy of patients with newly diagnosed AML.急性髓系白血病细胞中溶酶体膜蛋白LAMP1、TPC1、TPC2的表达及其临床意义.研究溶酶体膜蛋白LAMP1、TPC1、TPC2在急性髓系白血病（acute myeloid leukemia，AML）细胞中的表达变化与其临床指征之间的关系，初步探讨3种膜蛋白在AML发生发展中的可能作用及临床意义.采用实时荧光定量PCR方法检测AML细胞系（HL-60、NB4）、57例AML（其中初治患者44例，难治复发患者13例）LAMP1、TPC1、TPC2 的mRNA 的表达水平，并分析其与临床指标相关性和患者化疗后缓解情况的关系.与CD343种膜蛋白的mRNA在AML患者细胞中高表达，其中LAMP1、TPC1是AML疾病进展的危险因素，TPC2的高表达则有利于初治AML患者的化疗.

To discuss the hemiarthroplasty for the threatment of femoral intertrochanteric fracture in elderly patients.From August 2013 to January 2017, 66 elderly patients with complicated femoral intertrochanteric fracture were treated including 20 males and 46 females with an average age of 85 years old ranging from 80 to 95. According to improve Evans classification, 33 cases were type III, 24 cases were type IV, 9 cases were type V. History included 31 cases of hypertension, 10 cases of diabetes, 12 cases of severe osteoporosis, 5 cases of atrial fibrillation, 3 cases of pulmonary heart disease, and 1 case of multiple myeloma. Hip hemiarthroplasty was used in 34 cases and internal fixation in 32 cases. The bleeding volume, complications and Harris scores of the two groups were observed and compared.The amount of blood loss during and after operation were(320±20) ml and(200±25) ml in the joint replacement group, and (350±30) ml and(250±30) ml in the internal fixation group, and there was no statistical difference between the two groups(The elderly patients with femoral intertrochanteric fractures have many basic diseases, and the expected survival time is short. Using artificial bipolar femoral head prosthesis can restore the hip function early and to the maximum degree, make the patients get down in the bed early, effectively reduce the complications caused by internal fixation, improve the patient's quality of life and satisfaction, and early treatment. It is a kind of supplemental treatment, which conforms to the theory of rapid rehabilitation surgery and injury control, but should not expand the indication and strengthen the management of the perioperative period.

In order to investigate the role of otolith carbon (δ为探讨耳石碳(δ

To investigate the effect of microRNA-99a-5p (miR-99a-5p) on differentiation ability of human bone marrow mesenchymal stem cells (BM-MSC).BM-MSC was cultured and then transfected with miR-99a-5p mimics or inhibitors. The transfection efficiency was detected by real-time quantitative PCR. The effects of miR-99a-5p on the adipogenic and osteogenic differentiation ability of BM-MSC were detected by differentiation experiment.As compared with the negative control group, the expression of miR-99a-5p was significantly up-regulated after transfection with miR-99a-5p mimics(P0.001), the expression of miR-99a-5p was down-regulated after transfection with miR-99a-5p inhibitor (P0.001). In osteogenic differentiation experiments, the miR-99a-5p overexpression could promote the osteogenic differentiation, while the downregulation of miR-99a-5p expression inhibited the osteogenic differentiation. The same results were obtained by semi-quantitative detection through spectrophotometry. In the adipogenic differentiation test, transfection of miR-99a-5p mimics or inhibitors had no significant effect on the adipogenic differentiation of BM-MSC.Overexpression of miR-99a-5p can promote the osteogenic differentiation of BM-MSC, but no significant effects are observed in the adipogenic differentiation.

Comparison of four GPA cases with bilateral visual loss. In this file, we compared ophthalmological and MRI changes, pituitary function and treatment of 4 GPA cases with bilateral visual loss reported in literature. (DOCX 21 kb)

This article presents a wideband bandpass filter with controllable bandwidth and suppression of adjoining second passband, in which four same open loop resonators are adopted, hereinto two resonators located at the middle are integrated into a single trimode resonator using two microstrip lines. One line is applied to adjust desirable passband bandwidth, and the other is used to achieve the suppression of the second passband and obtain a wider stopband. Good return loss and inserted loss are obtained using an optimization routine. This technique has high flexibility on setting bandwidth and suppressing the adjoining second passband. Two prototype filters are designed and fabricated. Good agreements between simulation and measurement are demonstrated. © 2015 Wiley Periodicals, Inc. Microwave Opt Technol Lett 57:2939–2943, 2015

In this article, a new wideband bandpass filter (BPF) adopting two different types of resonators is proposed. The filter is constructed of four same open loop resonators and one inverted I-shaped resonator located at the middle. As a result, compared with conventional BPF without inverted I-shaped resonator, the proposed BPF exhibits one new transmission zero around the passband, which can adjust bandwidth and increase the selectivity at high-side edge of the passband significantly. The desired bandwidth can be obtained where the bandwidth is basically proportional to the upper or bottom coupling gaps between inverted I-shaped resonator and two adjoining open loop resonators. For verification, the proposed BPF is designed and fabricated where good agreement is observed. © 2015 Wiley Periodicals, Inc. Microwave Opt Technol Lett 57:1319–1323, 2015

Polysaccharides are one of the active components of Dendrobium officinale (D. officinale) and its content is used as one of the main quality assessment criteria. The existing methods for polysaccharide quantification involve sample destruction, tedious sample processing, high cost, and non-environmentally friendly pretreatment. The aim of this study is to develop a simple, rapid, green and nondestructive analytical method based on near infrared (NIR) spectroscopy and chemometrics methods. A set of 84 D. officinale samples from different origins was analyzed using NIR spectroscopy. Potential outlying samples were initially removed from the collected NIR data in two steps using the Monte Carlo sampling (MCS) method. Spectral data preprocessing was studied in the construction of a partial least squares (PLS) model. To eliminate uninformative variables and improve the performance of the model, the pretreated full spectrum was calculated using different wavelength selection methods, including competitive adaptive reweighted sampling (CARS), Monte Carlo-uninformative variable elimination (MC-UVE) and interval random frog (iRF). The selected wavelengths model met the following three points: (1) improved the prediction performance; (2) reduced the number of variables; (3) provided a better understanding and interpretation, which proves that it was necessary to conduct wavelength selection in the NIR analytical systems. When comparing the three wavelength selection methods, the results show that CARS has the best performance with the lowest root mean square error of prediction (RMSEP) on the independent test set and least number of latent variables (nLVs). This study demonstrates that the NIR spectral technique with the wavelength selection algorithm CARS could be used successfully for the quantification of the polysaccharide content in D. officinale.

Near-infrared spectroscopy coupled to chemometrics was used to develop a method to discriminate D. officinale, a high value herbal medicine, from non-D. officinale. Thirty-eight samples were analyzed with high performance liquid chromatography (HPLC) as a reference method. The near-infrared spectra were collected in reflectance mode. Spectral data were then processed by pretreatment methods and two chemometric classification techniques, principal component analysis (PCA) and hierarchical cluster analysis (HCA). Principal component analysis of near-infrared spectra and HPLC data revealed significant variation in D. officinale from other Dendrobium species. Moreover, a dendrogram based on hierarchical cluster analysis of the first derivative near-infrared spectra showed a chemical relationship among the samples. These results also approximately confirmed the results obtained by principal component analysis. In general, the results show that coupling of near-infrared spectroscopy to chemometric techniques is...

Aniline is considered as an environment hazard. Aniline-containing wastewater frequently has elevated salinity and high pH, which is generally not degraded by neutrophilic bacteria. The research explored the degradation ability of aniline by the novel isolated bacterial strain under alkaline and hyperhaline conditions. The strain was designated Bacillus sp. AN2 based on 16S rDNA sequence and Biolog Microstation Identification System. This strain had an unusually high salinity tolerance in minimal medium (0-6% NaCl, w/v). The optimal pH for microbial growth and aniline biodegradation was pH 9.0. For the initial concentrations of 200, 500, and 800 mgL-1, 95%, 74% and 62% of the aniline was degraded, respectively. The results suggest that the strain has potential for aniline degradation under alkaline and elevated salinity environmental conditions.

Selection of mechanical equipment is realized in the technical study on site. And according to different device powers and flow, relevant piping connections are perfected; then according to proportion of foaming agent developed in the lab, at the scene foaming equipment with compressed air is used to prepare foaming agent with good stability; then according to construction process, different dosage of early-strength admixture effecting the performance of compressive resistence of inorganic solidification foam materials in different time; at last, after the equipment being debugged well and relevant materials being prepared, inorganic solidification foam prepared to be 900 kg/m3 in the experiment is injected into baffle made from wood for solidifying; after 7 days, the dam constructed by this technology is detected with the result that there exists no apparent air leakage, forming a light closed-cell structure. The dam has a good performance of crack resistance and prevent air leakage.

To investigate the possible effect of MicroRNA-214 (miR-214) on migration of umbilical cord blood CD34After transfection of the inhibitor or mimic of miR-214, the cultured supernatant of BM-MSC were collected respectively. The expression of miR-214 in BM-MSC was detected by real time quantitative PCR, the effect of supernatant on CD34The cultured supernatant of BM-MSC could promote the migration of CD34miR-214 signalings may indirectly increase the migration of CD34

To investigate the therapeutic effect of minimally invasive interventional treatment of brain hematoma, 128 patients with brain hematoma were randomly divided into two groups, including observation group (64 cases) and control group (64 cases). The observation group was performed with minimally invasive interventional therapy and drug conservative therapy was used in the control group. Clinical curative effect, treatment of neural function defect score, and quality of life score were recorded. Total effective rate in observation group was significantly higher (p0.05) than that in control group (90.6% vs 62.5%). And the mortality in observation group was significantly lower (p0.05) than that in control group (3.1% vs 9.4%). National Institute of Health stroke scale (NIHSS) and Quality of Life (QOL) of the two groups after treatment were improved significantly (p0.05) compared with those with treatment before; and the improved level of concept in observation group was significantly higher (p0.05) than that of control group. Therefore, minimally invasive interventional brain hematoma surgery can effectively remove the hematoma in the brain, improve the nerve function of patients caused by trauma and improve the quality of life.

This article presents a high selectivity wideband bandpass filter (BPF) adopting stub loaded resonator. Hereinto, aim passband is determined by BPF without stub embedded, which is only composed of four half-wavelength open-loop resonators. Based on typical tapped-line coupling, two same stubs are located at physical middle points of two resonators connected with I/O feed lines, respectively. Due to embedded point at middle of loaded resonator, the stub with two open-end branches has no influence on original half-wavelength resonant frequency, and aim passband keeps unchanged. Because of different even-mode resonant frequencies between loaded and unloaded resonators, no new passband is constructed. With the help of embedded stubs, original transmission zero (TZ) near low-edge of aim passband is shifted towards passband, and a new TZ is introduced near high-edge. High selectivity and good passband characteristics are obtained optimizing sizes of stubs, I/O tapped position and top open-end length of loaded resonator.

This study reports the synthesis of bismuth ferrite ceramics (BiFeO3, BFO) by the sol-gel method and the effect of different sintering temperatures (800 °C, 820 °C, 850 °C, 880 °C, 900 °C) on the crystal structure, morphology, dielectric properties and ferroelectric properties of the bismuth ferrite ceramics. It is observed that the bismuth ferrite phase are formed when the molar ratio of bismuth and ferric is 1.025 : 1, and the bismuth ferrite ceramics have less impurity phase at all the five sintering temperature. The morphology studies revealed that the grain of bismuth ferrite ceramics is spherical and the grain size increases with the increasing of the sintering temperature. When the bismuth ferrite ceramics with excessive bismuth sintered at 820 °C, it exhibits the higher dielectric constant and the lower dielectric loss, the remnant polarization and the coercive field are 0.35 μC/cm2 and 4.03kV/cm, respectively. The observed ferroelectricity in bismuth ferrite ceramics owes to the point defects associated with oxygen vacancy defects as well as the smaller size effect.

To obtain an adjustable passband filter, one open stub with three branches is loaded on an open loop resonator. The first and the third passband frequencies of the tri-band filter can be flexibly controlled by tuning lengths of three branches, whereas the second passband frequency is fixed. The fabricated filter has good tri-passband performance at 2.19, 4.26, 6.30 GHz. Measured results agree well with the simulated ones.

As a new technique of photomicrography of complex optical field, the Fresnel incoherent correlation holography (FINCH) is particularly attractive in recent years because of its incoherent optical recording characteristics. For a new image recording and reconstruction system, a key concern is how to configure the experimental layout of FINCH by using available optical elements to achieve optimal resolution. However, in previous reports, there exist different viewpoints about this issue, and the imaging conditions of the best resolution remain to be clarified. As is well known, the imaging resolution is affected by the effective aperture of hologram and the change of the recording distance between spatial light modulator (SLM) and image sensor (CCD) can cause the hologram aperture to change. In the FINCH system the effective aperture of hologram is related not only to the aperture influence of each element used in the recording system, but also to the overlapping area of interference between the signal and reference wave and the pixel spacing of the image sensor. In previous reports, the researchers mainly used the ray-tracing method to discuss the effective aperture radius of hologram by ignoring the influences of the diffraction of light wave and the pixel spacing size of image sensor on the aperture of hologram. Based on the theories of wave optics we carry out a thorough investigation into the effective aperture of FINCH. We find that the pixelization of the image sensor, e.g. CCD, is a decisive factor influencing the resolution of FINCH, and we adopt numerical simulations and optical experiments to further verify the theoretical conclusions that the optimal lateral resolution of FINCH is achieved only if the recording distance (Zh) is equal to the focal length (fd) of diffractive lens displayed on a spatial light modulator; the resolution is deteriorated with the increase of \begin{document}$\left| {{Z_{\rm{h}}} - {f_{\rm{d}}}} \right|$\end{document}. From the viewpoint of Fourier optics, the smaller the imaging distance \begin{document}$\left| {{Z_{\rm{h}}} - {f_{\rm{d}}}} \right|$\end{document}, the larger the aperture angle of hologram (\begin{document}$ \approx {{{R_{\rm{h}}}} / {\left| {{Z_{\rm{h}}} - {f_{\rm d}}} \right|}}$\end{document}), the higher the collected spatial frequency is, hence, the higher the lateral resolution is. On the other hand, although the FINCH overcomes the spatial coherence limitation, it requires temporally coherent or quasi-monochromatic light. Our study also indicates that the requirements for the spatiotemporal coherence can be eased when the CCD is located at the focal plane of diffractive lens.

A compact bandpass filter has been investigated in this paper, the filter has four stepped impedance resonators, six open stubs are loaded on the two inner resonators, by adjusting sizes of six open stubs, a wide passband can be obtained. This proposed filter contributes respectively one transmission zero on either side of the wide passband. The proposed topology is demonstrated with a design operating at 5.205 GHz with 20.2% bandwidth, and more than 20dB of spurious suppression from 0 to 4.26 GHz and from 6.27 to 8.85 GHz is demonstrated in experimental results, which match well with the simulated results.

This research conducted chemical modification of foaming agent KH first, then determined the ratio of ingredients KH: SK: BH: SF: water of the foaming composite as 10:2:1:2:20 through orthogonal experiments, and made tests for relevant properties of samples at the said ingredient ratio and with dry density of 800 kg/m3, 900 kg/m3 and 1000 kg/m3 respectively determined by optimum seeking method via experiments. The test results show that the initial compressive strength of the material and its compressive strength after curing for 28 days are both relatively high.

We demonstrate the plasmon hybridization in a meta-molecules array composed of three identical split ring resonators (SRRs) in each unit. A plasmon hybridization resonant mode characterized by an admixture of electric dipole and quadrupole is observed, which origins from the electromagnetic interactions among the meta-atoms. This resonant mode is sensitive to the variation of spatial inversion symmetry determined by SRRs arrangement. Particularly, it can be tuned by changing the configuration of unit cell. The result means the spatial inversion symmetry of unit cell strongly influence the plasmonic coupling in the trimerical structure, offering an additional degree of freedom to control the electromagnetic interaction in metamaterials.