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Supplemental material, sj-pdf-1-imr-10.1177_03000605211055384 for Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit by Sheng Huang, Kangli Xu, Shanshan Xu, Shijun Yu, Yan Miao and Juan Wang in Journal of International Medical Research

Horizontal docking assembly is a fundamental process in the aerospace assembly, where intelligent measurement and adjustable support systems are urgently needed to achieve higher automation and precision. Thus, a laser scanning approach is employed to obtain the point cloud from a laser scanning sensor. And a method of section profile fitting is put forward to solve the pose parameters from the data cloud acquired by the laser scanning sensor. Firstly, the data is segmented into planar profiles by a series of parallel planes, and ellipse fitting is employed to estimate each center of the section profiles. Secondly, the pose of the part can be obtained through a spatial straight line fitting with these profile centers. However, there may be some interference features on the surface of the parts in the practical assembly process, which will cause negative effects to the measurement. Aiming at the interferences, a robust method improved from M-estimation and RANSAC is proposed to enhance the measurement robustness. The proportion of the inner points in a whole profile point set is set as a judgment criterion to validate each planar profile. Finally, a prototype is fabricated, a series of experiments have been conducted to verify the proposed method.

Acute Graft-Versus-Host Disease (aGVHD) is the major problem for patient undergoing allogeneic Hematopoietic Stem Cell Transplantation (allo-HSCT). Previous study showed the significant role of CD4+CD25+ Treg cells in inhibiting aGVHD. This retrospective study of 50 children with hematological malignancies undergoing allo-HSCT investigated the influence of donor CD4+CD25+CD127- Treg cells on aGVHD. The proportion of Treg cells in graft is significantly higher in patient with grade 0-I aGVHD than in patients with grade II-IV aGVHD (3.08 ± 0.72% vs. 2.52 ± 0.86%, P=0.016). There was no significant difference on Treg cells proportion in graft between relapsed and non relapsed patients (3.20 ± 0.80% vs. 2.80 ± 0.81% P=0.549). CD4+CD25+CD127- Treg cells in donor graft can reduce the incidence of aGVHD after children received allo-HSCT without increasing the risk of relapse. Graft CD4+CD25+CD127- Treg cells level is a valuable biomarker to predict aGVHD.