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A real-time imaging recognition and positioning method based on visible light communication flat light source is proposed. This method images the visible light communication flat light source through the rolling shutter effect of the complementary metal-oxide semiconductor imaging sensor and obtains the rectangular area outline of the light source. The light and dark stripe information of image with the digital image processing method realizes light source matching recognition by defining the concept, the autocorrelation sequence, which can be used to obtain the identity of the light source, and the rectangular vertex coordinate information of flat light source achieves high-precision vision positioning on the basis of inertial measurement unit attitude sensor-assisted imaging. Simultaneously, the corresponding positioning module is developed for positioning testing. The test results indicate that the plane positioning error is less than 4.5 cm, and the positioning frequency is greater than 10 Hz, which provides a high-precision visual positioning solution for indoor positioning.

Global navigation satellite system (GNSS) spoofing technology is an effective way to protect sensitive facilities and control foreign objects. To realize effective distributed GNSS forwarding spoofing for multiple receiver sensors in the area, the following research work is carried out: first, the GNSS forwarding spoofing model is established, including a forwarding spoofing mathematical model and an asynchronous traction spoofing method; second, the function scope of forwarding spoofing is fully evaluated; third, two forwarding spoofer area deployment algorithms for multi-spoofer multi-target (MSMT) are proposed—the multi-target same-point spoofing algorithm (MSPSA) is suitable for the same-point spoofing of multiple receiver sensors, and the multi-target different-point spoofing algorithm (MDPSA) is suitable for the different-point spoofing of multiple receiver sensors; and four, the experimental tests of MSPSA and MDPSA for MSMT were carried out. The experimental results show that MSPSA and MDPSA can respectively give the most appropriate deployment scheme of spoofing according to the various needs of the spoofer. For example, the number of spoofing devices is 1, the total distance between each spoofer and each receiver is 40,345.1 m, and the critical spoofing rate (CSR) and spoofing success rate (SSR) are both 100%. The performance of the spoofing deployment scheme can meet the needs of the spoofer.

When satellite navigation terminal sensors encounter malicious signal spoofing or interference, if attention is not paid to improving their anti-spoofing ability, the performance of the sensors will be seriously affected. The global navigation satellite system (GNSS) spoofing has gradually become a research hotspot of the jammer because of its great harm and high concealment. In the face of more and more sensors coupling GNSS and inertial measurement unit (IMU) to varying degrees and configuring a variety of anti-spoofing techniques to effectively detect spoofing, even if the spoofer intends to gradually pull the positioning results, if the spoofing strategy is unreasonable, the parameters of the coupled filter output and spoofing observation measurement will lose their rationality, which will lead to the spoofing being detected. To solve the above problems, in order to effectively counter the non-cooperative target sensors of assembling loosely coupled GNSS/IMU using GNSS spoofing, based on the analysis of the influence mechanism of spoofing on the positioning of loosely coupled GNSS/IMU, a slowly varying spoofing algorithm to avoid loosely coupled GNSS/IMU with multiple anti-spoofing techniques is proposed in this paper, and a measurement deviation determination method to avoid multiple anti-spoofing techniques is proposed, which can gradually pull the positioning results of the coupled system and successfully avoid the detection of anti-spoofing techniques of innovation sequence monitoring and a rationality check on parameters. Simulation experimental results show that the proposed algorithm gradually changes the positioning of loosely coupled GNSS/IMU, the north and east displacements achieve the purpose of spoofing, and error with expected offset is −0.2 m and 2.3 m, respectively. Down displacement also basically achieves the purpose of spoofing, and error with the expected offset is 13.2 m. At the same time, the spoofer avoids the detection of multiple anti-spoofing techniques, does not trigger the system alarm, and realizes the purpose of spoofing; thus, the effectiveness and high concealment of the spoofing algorithm are verified.

As Global Navigation Satellite System (GNSS) spoofing techniques are highly stealthy and pose a tremendous risk to targets using GNSS technology, studies on GNSS spoofing techniques have been in the spotlight. If the accurate position and velocity of the target receiver can be obtained, the target receiver can be covertly spoofed during the signal tracking stage using synchronous lift-off spoofing. However, it is often difficult to accurately obtain the position and velocity of a target in real GNSS spoofing scenarios. To address this problem, To study the effects of spoofing signals' power (relative to the real signal), code pulling rate, carrier Doppler shift, initial code phase difference, and carrier phase difference on the efficacy of spoofing, the intrusion of receiver's signal tracking loop by spoofing signals is mathematically modeled. Based on the model, an asynchronous lift-off spoofing for GNSS receivers in the signal tracking stage is proposed. Theoretical analysis and experimental results show that the new method resulted in stable Doppler frequency variations, short fluctuations in carrier-to-noise ratio (C/N) and signal lock time, and gentle changes to the receiver's 3D Earth-Centered Earth Fixed (ECEF) coordinates, when the target's position and velocity were approximately known during the intrusion period. The proposed spoofing method is highly feasible and could expand the scope of applicability of lift-off spoofing.

The value range of the failure threshold will generate an uncertain influence on the prediction results for the remaining useful life (RUL) of equipment. Most of the existing studies on the RUL prediction assume that the failure threshold is a fixed value, as they have difficulty in reflecting the random variation of the failure threshold. In connection with the inadequacies of the existing research, an in-depth analysis is carried out to study the effect of the random failure threshold (RFT) on the prediction results for the RUL. First, a nonlinear degradation model with unit-to-unit variability and measurement error is established based on the nonlinear Wiener process. Second, the expectation-maximization (EM) algorithm is used to solve the estimated values of the parameters of the prior degradation model, and the Bayesian method is used to iteratively update the posterior distribution of the random coefficients. Then, the effects of three types of RFT constraint conditions on the prediction results for the RUL are analyzed, and the probability density function (PDF) of the RUL is derived. Finally, the degradation data of aero-turbofan engines are used to verify the correctness and advantages of the method.

Aiming at the problem that the traditional teaching mode takes teachers as the center, students lack the ability of active learning and flexible application. According to the characteristics of online teaching, this paper applies the BOPPPS model to Rain Class integrating Ding talk, Wechat group and XuetangX platform. The organization methods of bridge-in, learning objective, pre-assessment, participatory-larning, post-assessment, and summary are designed elaborately. The practice shows that under the background of online teaching, the teaching mode proposed in this paper improves the students’ initiative in learning the course of "Systems engineering" and achieves the ideal teaching effect.

e16084 Background: The introduction of immune checkpoint inhibitors (ICIs) after chemotherapy and targeted therapy has altered the treatment pattern of esophageal squamous cell carcinoma (ESCC). However, using ICIs alone results in a poor response rate. Fortunately, fundamental research indicates that chemotherapy might trigger immunogenic death of tumor cells by releasing tumor antigens, hence eliminating immune system repression of tumor cells. On this basis, a number of clinical studies, including KEYNOTE-590, CheckmMate-648, ORIENT-15, and ESCORT-1st, on the first-line treatment of ESCC with ICIs coupled with chemotherapy, including fluorouracil or taxol and platinum (PF or TP), were conducted and yielded favorable results. However, the advantage of safety and effectiveness of ICIs combination with PF and TP in Chinese ESCC remains unknown. Furthermore, since the southeast part of Shanxi province has a high prevalence of esophageal cancer, the geographical features of ESCC patients will be examined. Methods: Camrelizumab was maintained after 6 cycles of camrelizumab in combination with PF or TP chemotherapy. From May 2020 to February 2022, our study has included 40 locally progressed and advanced ESCC patients. Camrelizumab was administered in combination with PF to 11 patients and TP to 29 patients. Every 6 weeks, efficacy was examined using RECIST 1.1, and 33 patients were evaluated. This research was registered with the Chinese Clinical Trials Registry (ChiCTR2000037942). Results: The median treatment time was 5.8m. 82.5% (33/40) patients were availably evaluated. The objective response rate (ORR) was 72.7% (24/33) and the disease control rate (DCR) was 97.0% (32/33). There is no statistically significant difference in ORR ( p=0.1779) and DCR ( p=0.1005) between PF and TP (55.6% vs 79.2%, 88.9% vs 100%) regimens. At this moment, mPFS has not been achieved. The most common adverse events (AEs) were anemia (22.5%, 9/40), lymphocytopenia (15%, 6/40), neutropenia(15%, 6/40), reactive cutaneous capillary endothelial proliferation (RCCEP) (12.5%, 5/40) and fatigue (7.5%, 3/40). Thrombocytopenia (2.5%, 1/40), neutropenia (2.5%, 1/40) and leukopenia (2.5%, 1/40) were the most common grade 3 or 4 toxicities The most frequently reported immune-related AEs were RCCEP (12.5%, 5/40) and hypothyroidism (7.5%, 3/40). There were no new significant adverse events. Conclusions: Camrelizumab in combination with chemotherapy is a promising regimen with good tolerability in the first-line treatment of ESCC. Compared with FP, TP had more therapeutic advantages, But the result is a stage summary, and further observation is needed. Clinical trial information: ChiCTR2000037942. [Table: see text]

3147 Background: Immune checkpoint inhibitors have opened a new chapter in cancer therapy, but the incidence of irAEs caused by them is high, and severe irAEs can be fatal. The current research on irAEs is almost focused on early predictions, and there is a lack of near-term predictions (the cycle before the occurrence of irAEs). Absolute eosinophil count (EO#) has been reported to be associated with immune-related pneumonia, but its association with other systemic irAEs requires further exploration. The aim of this study was to explore the near-term predictive value of neutrophil/lymphocyte (NLR), platelet/lymphocyte (PLR), and EO# for PD-1 inhibitor-induced irAEs. Methods: The data are from tumor patients who received PD-1 inhibitor therapy in our department from July 2019 to May 2021. A total of 146 cases were included, of which 56 had irAEs. The data of NLR, PLR and EO# in the cycle before the occurrence of irAEs (the median number of cycles was the second cycle) were collected, and the data of the second cycle was used as the control for patients without irAEs group. Logistic method was used to analyze the correlation between NLR, PLR and EO# and irAEs, and a predictive model was constructed. The sensitivity and specificity of the model were evaluated by ROC curve. This study was registered on Chinese Clinical Trail Registry (ChiCTR2100049849). Results: A total of 146 tumor patients were included, of which 56 developed at least one irAEs. Grade 1-2 irAEs occurred in 39 cases, grade 3-4 in 12 cases (including cardiac, liver, lung and skin toxicity), grade 5 in 2 cases(including cardiac and lung toxicity), and ungraded in 3 cases. The data of the cycle before the occurrence of irAEs were analyzed. Univariate analysis showed that NLR (odds ratio [OR], 1.4, p< 0.05) and EO# (OR, 12.6, p< 0.05) were associated with irAEs, and multivariate analysis suggested NLR (OR, 1.7, p< 0.001) and EO# (OR, 20.4, p< 0.05) were independent risk factors for irAEs. The prediction model composed of NLR, PLR and EO# had a correct rate of 76.7% (AUC = 0.752) in predicting the occurrence of irAEs in the near-term cycle, with a sensitivity of 51.8% and a specificity of 92.2%; the correct rate of predicting irAEs of grade 3 and above was as high as 91.9% (AUC = 0.778), the sensitivity was 14.3% and the specificity was 99.2%. Conclusions: The model composed of NLR, PLR and EO# may predict the occurrence of irAEs in the near-term cycle, especially the prediction of irAEs above grade 3, which can provide early warning for the occurrence of irAEs. Clinical trial information: ChiCTR2100049849.

Firstly this paper introduces the basic concept of stream computing, studies the relevant characteristics of stream data and analyzes the differences between the stream processing system and the traditional data processing system in detail. Secondly, the composition, operation mechanism and characteristics of the typical stream computing system storm are analyzed in detail and the structure of storm cluster is studied. Finally, the workflow of storm cluster architecture is studied.

Flipped classroom is an effective teaching method to improve teaching efficiency and quality. However, flipped classroom can easily lead to the defects of fragmentation of teaching process and polarization of learning effect, which hinders its effective application in online teaching. Task-driven teaching has the advantages of clear tasks and systematic knowledge, which can make up for the shortcomings of online teaching in flipped classroom. Aiming at the characteristics of online teaching under COVID-19 epidemic, this research carried out the design of task-driven online flipped classroom teaching model and explored practice. Based on the comprehensive use of multi-dimensional information platform, the situational design aroused and motivated the interest of students. The continuously upgrading tasks lead students to realize the conversion of knowledge mastery to ability formation. The effectiveness of online learning is comprehensively evaluated to realize the healthy operation of the teaching process and finally achieve the teaching goals.

Accumulation of the global paleomagnetic data, from both continental and oceanic plates, may suggest a true polar wander (TPW) event in Jurassic, with a rotation axis located in the present northwestern Africa, but no consensus has been reached regarding to the initiation, duration and velocity of the TPW. As one of the eastern Asian blocks, the north China block (NCB) is then located far from the rotation axis of the TPW and the plate convergence between Siberia and the Amur-NCB, known as the subduction in the Mesozoic Okhotsk-Baikal ocean, did exist. Paleogeographic changes observed of the eastern Asian blocks in Jurassic thus should contain the TPW component and plate moving component. To better estimate the influence of the TPW in the Eastern Asia blocks, we carried out a new paleomagnetic and precision U-Pb geochronological study on the middle Jurassic lavas in the NCB. Being profoundly different to the recent paleogeographic model (Yi et al., 2019, https://doi .org/10.1130/G46641.1) that suggest that the NCB experienced a large latitudinal displacement (monster-shift) responding to the TPW event between ~174 and ~157 Ma, we suggest that the NCB, as well as other blocks already connected with it, do not record any monster-shift between ~170 and ~160 Ma. The strata, ranging from 160 to 145 Ma, however, yield considerable paleomagnetic variations and need further investigation.

Fossil organisms of the Boreal Realm in the Tuva–Mongol Block (TMB) and Amuria Block (AMB) are commonly thought to have lived at mid to high latitudes during the Carboniferous Period; however, reliable paleomagnetic constraints have not been available previously to test this hypothesis. Here we report newly obtained combined paleomagnetic and geochronological results from the Upper Mississippian Gunbayan Formation in the AMB and the Lower Pennsylvanian Altan-Ovoo Formation in the TMB. Zircon U–Pb dating of tuff beds from the Gunbayan and Altan-Ovoo formations yield ages of 331.0 ± 2.7 and 315.5 ± 2.4 Ma, respectively. A total of 263 paleomagnetic specimens underwent stepwise thermal demagnetization. After removing the viscous remanent magnetizations of the recent geomagnetic field, stable high-temperature components (HTCs), carried by magnetite, were successfully acquired from 240 specimens. The HTCs of the Gunbayan Formation passed a fold test and a reversal test, and those of the Altan-Ovoo Formation passed a fold test, suggesting that they represent primary remanence magnetizations. We used the elongation/inclination method to test and correct for all the HTC directions of the clastic rocks of the studied formations. Their corresponding paleomagnetic poles are 46.0°N/320.0°E (A = 95 2.0 °) at ca. 330 Ma for the AMB and 43.5°N/355.9°E (A = 95 2.9 °) at ca. 315 Ma for the TMB. The updated paleomagnetic database indicates with certainty that both the TMB and the AMB were located in northern hemisphere low-latitude regions and close to the equator at ca. 330 Ma, suggesting that the Boreal Realm might have extended into the equatorial region at the onset of the large ice age of the Carboniferous. The paleobiogeographic characteristics and distribution of climate-sensitive lithologies indicate that this realm occupied a wide northern temperate belt during 330–315 Ma.

Jurassic witnessed two synchronous events on the Earth, named true polar wander (TPW) and closure of the Mongol–Okhotsk ocean (MOO) between Siberia and eastern Asian blocks. The paleogeographic change of the north China block (NCB) should thus include both TPW and plate tectonic components in Jurassic. We performed a new combined paleomagnetic and U–Pb geochronological study for Middle and Late Jurassic volcanic rocks from the northern NCB and obtained three new well-dated paleopoles: 82.7°N, 282.6°E, A 95 = 3.2 ° (ca. 160 Ma); 84.1°N, 228.9°E, A 95 = 6.6 ° (ca. 165 Ma); and 89.3°N, 216.8°E, dp/dm = 5.4°/7.1° (ca. 170 Ma). A positive untilting test indicates a pre-folding origin interpretation for the ca. 160 Ma pole. Quality of these poles is enforced by three positive conglomerate tests, the presence of magnetic reversals and secular variation analyses. Comparison of the apparent polar wander paths of Eurasia and the NCB suggests that the NCB accreted to Eurasia around 140 Ma. The NCB and its then connected blocks did not undergo any significant southward paleogeographic shift during Jurassic. In context of TPW, we estimate a net northward plate moving velocity at 0.50–0.75°/myr for the NCB in Jurassic. The long-term accumulation of MOO subducted slabs at the high paleolatitudes may have critically contributed to, or perhaps led to, the Jurassic TPW.

Network repair strategies are tactical methods that restore the efficiency of damaged networks; however, unreasonable repair strategies not only waste resources, they are also ineffective for network recovery. Most extant research on network repair focuses on static networks, but results and findings on static networks cannot be applied to evolutionary dynamic networks because, in dynamic models, complex network repair has completely different characteristics. For instance, repaired nodes face more severe challenges, and require strategic repair methods in order to have a significant effect. In this study, we propose the Shell Repair Strategy (SRS) to minimize the risk of secondary node failures due to the cascading effect. Our proposed method includes the identification of a set of vital nodes that have a significant impact on network repair and defense. Our identification of these vital nodes reduces the number of switching nodes that face the risk of secondary failures during the dynamic repair process. This is positively correlated with the size of the average degree 〈 k 〉 and enhances network invulnerability.

Rationale Pain is the fifth vital sign of human beings. Morphine is the first choice for relieving moderate to severe cancer pain. Most of the previous studies merely focused on the analgesic effect of high-dose or ultra-high-dose morphine in patients with advanced cancers but did not report any cases related to successful morphine withdrawal. Patient concerns A 42-year-old woman was admitted to our hospital in March 2019. Diagnosis She was diagnosed with progressive aggravation of headache for 1 month, which was meningeal metastasis of lung cancer. Interventions Symptomatic treatments like dehydration, hormone, intrathecal injection chemotherapy and an increased dose of osimertinib to 160 mg/day were applied but showed poor curative effects. The patient refused whole-brain radiotherapy. Pain intensity level was re-evaluated and the patient scored 9 based on numerical rating scale, which suggested that the patient suffered from severer cancerous pain. Thus, the patient started to receive morphine for treating headache. Outcomes The patient's headache was alleviated after receiving high-dose morphine treatment, and she continued to undergo anti-cancer treatment. After tumor remission, the patient's morphine dose gradually decreased and eventually stopped, without any withdrawal symptoms. In addition, the quality of life of the patient was greatly improved with performance status scored 2 and limb muscle strength increased from Grade 2 to Grade 5. Lessons For patients with advanced cancers, the application of ultra-high-dose morphine may significantly relieve cancerous pain, improve survival and quality of life, and overcome their fear for death and desperation, which contributes to the establishment of a basis for subsequent anticancer treatments. Thus, timely effective pain management and routine anticancer treatments are the key to addressing the cancer pain problem.

The carrier-based aircraft system is the core component of modern aircraft carrier combat system, a maritime platform for carrier-borne fighters, whether carrier aircraft can demonstrate combat power depends on the ability of the carrier-based aircraft to take off and landing. In order to improve the safety and reliability of shipboard on warship, this paper aims at GNSS/INS/Photogrammetry integrated navigation theory, discussed from the basic level of integrated navigation system on implementation and the performance of the whole positioning. The mainly research is when satellite is not available, the inertial navigation error is corrected by the positioning of the Photogrammetry, to achieve high accuracy, high reliability and data refresh rate. The multi-rate kalman filter method is used to deal with the problem of the non-equal intervals between the INS and Photogrammetry. Finally, by simulating the accuracy of the integrated navigation positioning of INS/Photogrammetry by multi-rate EKF filter, to verify the feasibility of the aircraft landing/carrier landing.

Colorectal cancer (CRC) is the most common malignant tumor type and has become resistant to 5-fluorouracil (5-FU) in recent decades, which is one of the most popular therapies. Recently, microRNA (miRNA or miR) has been investigated as a potential therapeutic strategy for CRC. However, there has been little investigation of the underlying mechanism of the association between expression of miRNA and chemosensitivity. The present study aimed to investigate the effect of miR-1260b inhibitor on CRC cells, and their chemosensitivity to 5-FU, by treating them with the miR-1260b inhibitor. miR-1260b inhibitor was demonstrated to significantly promote the proliferation and invasion of the CRC cell line, HCT116, and to increase the apoptotic rate. Furthermore, it was validated that programmed cell death 4 (PDCD4) was a direct target of miR-1260b inhibitor in CRC with bioinformatics tools and a luciferase assay. Western blot analysis revealed that miR-1260b inhibitor could significantly decrease PDCD4 expression, and downregulate the expression of phosphorylated-Akt (p-Akt) and phosphorylated-extracellular-signal-regulated kinase (p-ERK). In conclusion, it was confirmed that the anti-tumor effect of the miR-1260b inhibitor was conducted by blocking the phosphorylated 3-kinase/Akt pathway as dysregulated protein expression induced by miR-1260b inhibitor was rescued by insulin-like growth factor. Notably, miR-1260b inhibitor could significantly enhanced the chemoresponse of HCT116 cells to 5-FU via reduced proliferation, increased apoptosis, and downregulation of PDCD4, p-Akt and p-ERK protein expression. In summary, the present study may provide a novel direction for future clinical therapy to enhance the chemosensitivity of tumor cells.

Random walk plays an important role in heuristic algorithm. The traditional Cuckoo Search used only Levy random stemmed from Levy distribution. This paper extends two symmetric random walk methods, named Gauss random and Cauchy random, which are consistent with α-stable distribution and can be applied to the Cuckoo Search. The characteristics of three random methods are compared and analyzed, and the Cauchy random is revised. Based on the infrastructure framework of the Cuckoo Search algorithm, two new Cuckoo Search modes using new random walk methods are constructed. By comparing with the original Cuckoo Search in solving benchmark functions optimization and pressure vessel design problem, the conclusions can be drawn that Cauchy random has a great advantage for low dimensional problems and Gauss random is quite competitive for multidimensional unimodal problems, while Levy random is suitable for multidimensional and multimodal problems. And it has certainly guiding significance for the optimization design of algorithm and some optimization problems.

Because certified reference standards on molybdenum (Mo) in soil are not available, and drafted risk assessment procedures in China have not included Mo. Assessment of the potential risks posed by Mo in soil and groundwater is the key to establishing the extent of the contamination and deriving achievable remedial targets, should remediation deemed to be necessary. This paper reports the first investigation of Mo contamination in soil and groundwater from an incandescent light-bulb manufacturing facility in China. Though the plant was built in 1971, the contamination of Mo in the site received little attention until the 1994 and 2008 monitoring campaigns; soil Mo concentrations ranged from not-detectable to 15 mg/kg in 1994, and from 0.25 to 252 mg/kg in 2008. In addition, groundwater Mo concentrations ranged from not-detectable to 362 μg/L in 1994, and 1 to 32, 500 μg/L in 2008. Simulation by Visual MINTEQ software showed that Mo speciation in the groundwater would be limited to MoO42 −. Detailed site investigation showed that the high concentrations of Mo in groundwater could be adequately explained by the predominant presence of anionic MoO42 − at the measured average soil pH of 8.65, and given the small adsorption coefficient 112 L/kg for Mo onto field soil samples. A modified three-step sequential extraction procedure showed that residual percentages of soil Mo at this industrial site ranged from 69.8 to 81.0%, indicating that Mo in the soil was mostly present in the mineral lattice. Bounding of Mo onto the mineral lattice is not available to living organisms and thus the risk from soil contamination was considered minimal. A conceptual site model developed for quantitative risk assessment indicated that the main exposure pathways would be consumption of the groundwater by local residents, and lateral Mo migration to bounding rivers. Only non-carcinogenic risk was assessed, because Mo has no known carcinogenic effect on living organisms, as indicated from the toxicity data for Mo. Among the 35 assessment locations, Mo in 14 locations was determined to pose unacceptable non-cancer risk for on-site children, with a hazard quotient from 1.2 to 433 when children directly drink groundwater. In addition, Mo in groundwater which is transported off-site by lateral migration will pose unacceptable non-cancer risks for off-site children, with a hazard quotient of up to 45.8 from direct water drinking. Although Mo concentrations in the bounding rivers ranged from 4 to 6,053 μg/L, toxicity data indicated that the ecological risk is minimal for aquatic biota in the surface water. A site-specific target level for Mo in groundwater was established as 75.1 μg/L. Further work will be conducted regarding remediation feasibility at this site; permeable reactive barriers may be an effective option given the predominant distribution of Mo in the groundwater in the site.

Our previous study has shown that downregulation of B-cell chronic lymphocytic leukemia (CLL)/lymphoma11A (BCL11A) gene by small interfering RNA (siRNA) resulted in the growth inhibition and apoptosis of B cell lymphoma cell line SUDHL6. To gain further insight into the molecular mechanisms of this process and identify the differentially expressed genes in SUDHL6 cells after BCL11A downregulation, the global gene expression profile was identified and analyzed using the Affymetrix HG-U133 Plus 2.0 array. Twenty-one differentially expressed genes were validated and analyzed from the BCL11A siRNA-treated SUDHL6 cells. There was a significant dysregulation in the global gene expression of the BCL11A-suppressed SUDHL6 cells. There were 1903 genes differentially expressed with >2-fold changes between the BCL11A siRNA- and negative control-transfected cells. Of these, there were 916 upregulated genes and 987 downregulated genes. The differential genes are involved in various molecular functions and signaling pathways. QRT-PCR validation of the selected differentially expressed genes demonstrated there was a good correlation with the microarray analysis. There was a significant deregulation of expression in the apoptosis-related genes such as BCL-2, BCL2L11 and involved in TGFβ, MAPK, WNT signaling pathways after BCL11A was downregulated in SUDHL6 cells. Our results show that the suppression of BCL11A by RNA interference altered gene expression profile of SUDHL6 cells. The apoptosis-related genes BCL-2, BCL2L11 and the gene alterations in TGFβ, MAPK, WNT signaling pathways might be important in BCL11A siRNA-induced apoptosis of SUDHL6 cells, suggesting BCL11A is involved in gene networks associated with apoptosis.

The Amuria block occupies the eastern part of the Central Asian Orogenic Belt between the Siberia craton and the North China block (NCB) and bears important information to understand the evolution of the MongolOkhotsk suture and the amalgamation of East Asia. However, the paleomagnetic database of Amuria remains very poor.

OBJECTIVE The B-cell lymphoma/leukemia 11A (BCL11A) gene is essential for normal lymphoid development and has been associated with hematological malignancies. In the current study, the relative expression level of BCL11A in malignant hematological cell lines was evaluated through real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). METHODS The relative expression level of BCL11A mRNA in malignant hematological cell lines was determined through qRT-PCR using SYBR Green I dye. Glyceraldehyde-3-phosphate dehydro-genase was used as the reference gene to confirm the relative expression level of BCL11A gene mRNA. RESULTS The relative expression level of BCL11A mRNA in cell lines from B-cell malignancies was significantly higher compared with that from acute myeloid leukemia ( P

SDF-1α, a ligand for the chemokine receptor CXCR4, is well known for mediating the migration of breast cancer cells. In a previous study we demonstrated that a synthetic 21-mer peptide antagonist of CXCR4 (NT21MP) derived from the viral macrophage inflammatory protein II could antagonize tumor growth in vivo by inhibiting cellular proliferation and inducing apoptosis in breast cancer cells. However, the role of SDF-1α in the signaling pathways underlying the proliferation of human breast cancer cells and associated signaling pathways and inhibiting signal pathways of NT21MP remained unclear. The present study investigated the mechanism of NT21MP on anti-tumor in breast cancer in vitro. The effect of NT21MP on the viability of cells was determined by the MTT assay. Annexin V-FITC and PI staining was performed to detect early stage apoptosis in SKBR3 cells treated with SDF-1α and AMD3100 or NT21MP. Western blotting techniques were used to assay the composition of phosphoproteomics and total proteins present in the SKBR3 breast cancer cells. RT-PCR and Western blotting technique were used to detect the effect of NT21MP and AMD3100 on Bcl-2 and Bax expression. The results indicated that SDF-1α prevented apoptosis and promoted the proliferation of SKBR3 human breast cancer cells. As compared with untreated SKBR3 cells, Treatment with SDF-1α significantly increased cell viability, and NT21MP abolished the protective effects of SDF-1α dose-dependently (P

Our previous study has shown that downregulation of B-cell chronic lymphocytic leukemia (CLL)/lymphoma11A (BCL11A) gene by small interfering RNA (siRNA) resulted in the growth inhibition and apoptosis of B cell lymphoma cell line SUDHL6. To gain further insight into the molecular mechanisms of this process and identify the differentially expressed genes in SUDHL6 cells after BCL11A downregulation, the global gene expression profile was identified and analyzed using the Affymetrix HG-U133 Plus 2.0 array. Twenty-one differentially expressed genes were validated and analyzed from the BCL11A siRNA-treated SUDHL6 cells. There was a significant dysregulation in the global gene expression of the BCL11A-suppressed SUDHL6 cells. There were 1903 genes differentially expressed with2-fold changes between the BCL11A siRNA- and negative control-transfected cells. Of these, there were 916 upregulated genes and 987 downregulated genes. The differential genes are involved in various molecular functions and signaling pathways. QRT-PCR validation of the selected differentially expressed genes demonstrated there was a good correlation with the microarray analysis. There was a significant deregulation of expression in the apoptosis-related genes such as BCL-2, BCL2L11 and involved in TGFβ, MAPK, WNT signaling pathways after BCL11A was downregulated in SUDHL6 cells. Our results show that the suppression of BCL11A by RNA interference altered gene expression profile of SUDHL6 cells. The apoptosis-related genes BCL-2, BCL2L11 and the gene alterations in TGFβ, MAPK, WNT signaling pathways might be important in BCL11A siRNA-induced apoptosis of SUDHL6 cells, suggesting BCL11A is involved in gene networks associated with apoptosis.

Bcl-2 is a prominent member of the Bcl-2 family of proteins that regulate the induction of apoptosis. Here, we investigated the effect of Bcl-2 small interfering RNA (siRNA) combined with miR-15a oligonucleotides (ODN) on the growth of Raji cells. The expression levels of Bcl-2 mRNA and protein were detected. The inhibition of cell growth and apoptosis was determined. Atomic force microscopy was utilized to observe the cell-surface ultrastructural changes. After transfection of combination, protein and mRNA levels of Bcl-2 obviously were decreased. The growth of cells was significantly inhibited compared with those cells transfected with Bcl-2 siRNA or miR-15a alone. Apoptotic rate significantly increased. The cells of apoptosis showed a roughness variation on cell membranes. These results suggest that the combination of Bcl-2 siRNA and miR-15a ODN increases apoptosis in Raji cells. Our study implies that the combination of Bcl-2 siRNA and miR-15a may be a useful approach in treatment for lymphoma.

This study was to explore the induced differentiation of human mesenchymal stem cells (MSCs) modified by pancreatic and duodenal homeobox factor 1 (Pdx1) gene into insulin-producing cells in vitro. After recombined adenovirus vector with Pdx1 gene infected MSCs for 7 d, cells were induced by induction factors. The genes' expressions related to islet beta cells such as Pdx1, insulin, glucose transporter-2 (Glut2), were detected with RT-PCR, immunocytochemistry and Western blot. The levels of insulin and C peptide secretion were examined with chemiluminescence immunoassay. Insulin(+) cell rate was detected by flow cytometry. After infected by recombined adenovirus with Pdx1 and combined with induction factors, MSCs were aggregated and islet-like cell clusters formed. Dithizone staining of these cells was positive. The genes' expression related to islet beta cells, such as Pdx1, insulin, Glut2, could be detected. After induction, the islet-like cell clusters secreted insulin and C peptide. The levels of insulin and C peptide secretion increased with glucose stimulation. Insulin(+) cell rate was (11.61 +/- 4.83)%. It could be concluded that Pdx1 gene modified MSCs from human umbilical cord could be induced to differentiate into islet beta-like cells.

Mesenchymal stem cells (MSCs) have significant advantages over other stem cell types, and greater potential for immediate clinical application. MSCs would be an interesting cellular source for treatment of type 1 diabetes. In this study, MSCs from human umbilical cord were differentiated into functional insulin-producing cells in vitro by introduction of the pancreatic and duodenal homeobox factor 1 (PDX1) and in the presence of induction factors. The expressions of cell surface antigens were detected by flow cytometry. After induction in an adipogenic medium or an osteogenic medium, the cells were observed by Oil Red O staining and alkaline phosphatase staining. Recombinant adenovirus carrying the PDX1 gene was constructed and MSCs were infected by the recombinant adenovirus, then treated with several inducing factors for differentiation into islet β-like cells. The expression of the genes and protein related to islet β-cells was detected by immunocytochemistry, RT-PCR and Western blot analysis. Insulin and C-peptide secretion were assayed. Our results show that the morphology and immunophenotype of MSCs from human umbilical cord were similar to those present in human bone marrow. The MSCs could be induced to differentiate into osteocytes and adipocytes. After induction by recombined adenovirus vector with induction factors, MSCs were aggregated and presented islet-like bodies. Dithizone staining of these cells was positive. The genes' expression related to islet β-cells was found. After induction, insulin and C-peptide secretion in the supernatant were significantly increased. In conclusion, our results demonstrated that PDX1 gene-modified human umbilical cord mesenchymal stem cells could be differentiated into insulin-producing cells in vitro.

The study were carried out to select the macrophyte planting in floating bed, which could be applied to the ecological restoration of the estuary of Daqing river to Dianchi Lake.There are five indigenous emerging species (Carina generalisBailey, Cyperus Alternifolius, Typha angustifoliaL. Zizania caduciflera, Iris tectorum), cultured in the stressed conditions of the serious pollution river water. These used as the selecting indices are the removal rate of CODCr total phosphorus(TP), total nitrogen(TN), phosphate(PO4 3-), ammonia nitrogen(NH3-N), the ability of resistance oxygenation: MDA content, proline content and 1 - naphthylamine changes of root oxidation. Comprehensive analysis of the index show that five options plants have a big difference in the adaptability of pollution stress and the removal of pollutants, of which Canna and Iris have the best adaptability, and removal efficiency. Therefore, Canna and Iris could be chosen as ideal species in floating bed for the restoration.

Activated sludge process is the main technology for modern wastewater treatment. The principle and development of integrated dynamic simulation system for activated sludge process was finished here. This simulation system is mainly applied to predicting the performance of wastewater treatment based on the theory of simplified sludge model and one-dimension settling model of circular secondary settler. The application example shows that this simulation system is a valuable tool for the plant operator or designer in forecasting or explaining the performance of wastewater treatment.

A solution of wastewater treatment supervisory control and data acquisition for environment teaching is discussed here. Through the Internet/Intranet, clients can download control model to target PC to realize online data acquisition and operate hardware in remote laboratory. At the same time, it researches the mechanism of wastewater treatment process by combining simulation and control technique with domestic technical characteristic. The test results proves that the technical structure of the lab allows further addition of new control algorithms to solve and thereby vary the education process for the students. Thus, the implementation strategy has good expandability and flexibility.