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Additional file 1: Supplementary Figure 1. Venn diagram of common and differentially expressed mRNAs of gill tissues of abalone during short-term low salinity exposure: H. discus hannai (DD) and hybrid H. discus hannai ♀ × H. fulgens ♂ (DF). A) Comparisons within DD (CDvsD3, CDvsD24, D3vsD24), B) Comparisons within DF (CFvsF3, CFvsF24, F3vsF24), and C) Comparisons between DD and DF (CDvsCF, D3vsF3, D24vsF24). Controls: CD, CF; 3 h at low salinity exposure: D3, F3; and 24 h at low salinity exposure: D24, F24. Supplementary Fig. 2. Volcano plots of the differentially expressed genes (DEGs) of gill tissues of abalone during short-term low salinity exposure: I) H. discus hannai (DD): A. Comparison in DD between control (CD) and low salinity after 3 h (D3), B. Comparison in DD between control (CD) and low salinity after 24 h (D24). II) Hybrid H. discus hannai ♀ × H. fulgens ♂ (DF). A. Comparison in DF between control (CF) and low salinity after 3 h (F3) B. Comparison in DF between control (CF) and low salinity after 24 h (D24). Red color denotes up-regulated genes and Blue color denotes and down-regulated. Supplementary Fig. 3. Comparison of top 20 Kyoto Encyclopedia of Gene and Genome (KEGG) pathways enrichment statistics of gill tissues of abalone during short-term low salinity exposure. H. discus hannai (DD) and hybrid H.discus hannai ♀ × H. fulgens ♂ (DF). FSS pathway is highlighted with a rectangular red box. (I) Enrichment analysis for DD: A. Comparison between control (CD) and low salinity after 3 h (D3), B. Comparison between control (CD) and low salinity after 24 h (D24), and C. Comparison between low salinity groups after 3 h and 24 h (CD3vsD24). (II) Enrichment analysis for DF: A. Comparison between control (CF) and low salinity group after 3 h (F3), B. Comparison between control (CF) and low salinity group after 24 h (F24), and C. Comparison between low salinity groups after 3 h and 24 h (F3vsF24). The size of each point represents the number of genes annotated to the KEGG pathway. Different colors from yellow to mauve represent the p-value of the enrichment. Supplementary Table S1. Summary of RNA-Seq data quality analysis of H. discus hannai (DD) and hybrid H. discus hannai ♀ × H. fulgens ♂ (DF) during short-term low salinity exposure

A novel orange shell colour has been discovered in small abalone, Haliotis diversicolor (Reeve, 1846). To facilitate and accelerate a selective breeding programme for orange shell colour, a microsatellite marker that was completely linked to the shell colour gene was identified using a selective DNA pool approach and was verified in 448 individuals. Two DNA pools were constructed by pooling equal amounts of genomic DNA from 74 individuals with dark-brown shells and 87 individuals with orange shells. Approximately 12 of 308 microsatellite markers that were linked to the shell colour gene were isolated using the selective DNA pooling method. After verification in 32 individuals with dark shells and 32 individuals with orange shells, only the Hdi3-119 locus remained tightly linked to the shell colour gene. Finally, four families were used to verify the accuracy of the Hdi3-119 locus. All of the individuals with orange shells in the four families harboured a unique 381-bp allele in marker Hdi3-119. This marker could be used for selective breeding of orange small abalone.

In scalable video coding, inter-layer prediction is used to eliminate the redundancies among layers. Joint Scalable Video Model (JSVM) uses a fixed interpolation scheme to interpolate motion and residual information of the base layer into the enhancement layer. The filter used for luma and chroma information is also the same. In this paper, an adaptive interpolation scheme for inter-layer prediction in scalable video coding is presented. Firstly, the interpolation filter used for luma information is adaptively selected among three filters: bilinear, bicubic, ant 6-tap filter. The selection of interpolation filter is based on the quantization parameter. Secondly, bilinear filter is used for chroma information instead of the 6-tap filter used in JSVM. Experimental results show that, compared with the fixed interpolation scheme used in JSVM, the proposed scheme achieves satisfied results in both subjective and objective quality.