28 results on '"Yueyuan Liu"'
Search Results
2. Comparison of structural variants detected by PacBio-CLR and ONT sequencing in pear
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Yueyuan, Liu, Mingyue, Zhang, Runze, Wang, Benping, Li, Yafei, Jiang, Manyi, Sun, Yaojun, Chang, and Jun, Wu
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Pyrus ,Nanopores ,Genomic Structural Variation ,Genetics ,Chromosome Mapping ,High-Throughput Nucleotide Sequencing ,Sequence Analysis, DNA ,Sequence Analysis ,Genome, Plant ,Biotechnology - Abstract
Background Structural variations (SVs) have recently become a topic of great interest in the area of genetic diversity and trait regulation. As genomic sequencing technologies have rapidly advanced, longer reads have been used to identify SVs at high resolution and with increased accuracy. It is important to choose a suitable sequencing platform and appropriate sequencing depth for SV detection in the pear genome. Results In this study, two types of long reads from sequencing platforms, continuous long reads from Pacific Biosciences (PB-CLR) and long reads from Oxford Nanopore Technologies (ONT), were used to comprehensively analyze and compare SVs in the pear genome. The mapping rate of long reads was higher when the program Minimap2 rather than the other three mapping tools (NGMLR, LRA and Winnowmap2) was used. Three SV detection programs (Sniffles_v2, CuteSV, and Nanovar) were compared, and Nanovar had the highest sensitivity in detecting SVs at low sequencing depth (10–15×). A sequencing depth of 15× was suitable for SV detection in the pear genome using Nanovar. SVs detected by Sniffles_v2 and CuteSV with ONT reads had the high overlap with presence/absence variations (PAVs) in the pear cultivars ‘Bartlett’ and ‘Dangshansuli’, both of them with 38% of insertions and 55% of deletions overlapping with PAVs at sequencing depth of 30×. For the ONT sequencing data, over 37,526 SVs spanning ~ 28 Mb were identified by all three software packages for the ‘Bartlett’ and ‘Dangshansuli’ genomes. Those SVs were annotated and combined with transcriptome profiles derived from ‘Bartlett’ and ‘Dangshansuli’ fruit flesh at 60 days after cross-pollination. Several genes related to levels of sugars, acid, stone cells, and aromatic compounds were identified among the SVs. Transcription factors were then predicted among those genes, and results included bHLH, ERF, and MYB genes. Conclusion SV detection is of great significance in exploring phenotypic differences between pear varieties. Our study provides a framework for assessment of different SV software packages and sequencing platforms that can be applied in other plant genome studies. Based on these analyses, ONT sequencing data was determined to be more suitable than PB-CLR for SV detection in the pear genome. This analysis model will facilitate screening of genes related to agronomic traits in other crops.
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- 2022
3. Rearrangement and domestication as drivers of Rosaceae mitogenome plasticity
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Manyi Sun, Mingyue Zhang, Xuening Chen, Yueyuan Liu, Binbin Liu, Jiaming Li, Runze Wang, Kejiao Zhao, and Jun Wu
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Physiology ,Cell Biology ,Plant Science ,General Biochemistry, Genetics and Molecular Biology ,Domestication ,Evolution, Molecular ,Pyrus ,Structural Biology ,Genome, Mitochondrial ,General Agricultural and Biological Sciences ,Rosaceae ,Genome, Plant ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,Developmental Biology ,Biotechnology - Abstract
Background The mitochondrion is an important cellular component in plants and that functions in producing vital energy for the cell. However, the evolution and structure of mitochondrial genomes (mitogenomes) remain unclear in the Rosaceae family. In this study, we assembled 34 Rosaceae mitogenomes and characterized genome variation, rearrangement rate, and selection signal variation within these mitogenomes. Results Comparative analysis of six genera from the Amygdaloideae and five from the Rosoideae subfamilies of Rosaceae revealed that three protein-coding genes were absent from the mitogenomes of five Rosoideae genera. Positive correlations between genome size and repeat content were identified in 38 Rosaceae mitogenomes. Twenty repeats with high recombination frequency (> 50%) provided evidence for predominant substoichiometric conformation of the mitogenomes. Variations in rearrangement rates were identified between eleven genera, and within the Pyrus, Malus, Prunus, and Fragaria genera. Based on population data, phylogenetic inferences from Pyrus mitogenomes supported two distinct maternal lineages of Asian cultivated pears. A Pyrus-specific deletion (DEL-D) in selective sweeps was identified based on the assembled genomes and population data. After the DEL-D sequence fragments originally arose, they may have experienced a subsequent doubling event via homologous recombination and sequence transfer in the Amygdaloideae; afterwards, this variant sequence may have significantly expanded to cultivated groups, thereby improving adaptation during the domestication process. Conclusions This study characterizes the variations in gene content, genome size, rearrangement rate, and the impact of domestication in Rosaceae mitogenomes and provides insights into their structural variation patterns and phylogenetic relationships.
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- 2022
4. Genome-wide comparison of the GRAS protein family in eight Rosaceae species and GRAS gene expression analysis in Chinese white pear (Pyrus bretschneideri Rehder)
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Yueyuan Liu, Kejiao Zhao, Jiaming Li, Jiawen Sheng, Wenjing Chang, Mingyue Zhang, Zikai Tang, Jun Wu, Manyi Sun, and Bobo Song
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Genetics ,PEAR ,biology ,Protein family ,Rosaceae ,fungi ,food and beverages ,Horticulture ,biology.organism_classification ,Genome ,White (mutation) ,Gene expression ,human activities ,Agronomy and Crop Science ,Gene ,Transcription factor - Abstract
The GRAS protein family encodes a family of plant-specific transcription factors that have diverse and significant functions in regulating plant development and anthocyanin biosynthesis. Our study ...
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- 2021
5. An improved image dehazing method based on dark channel compensation and guided filtering
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Yueyuan Liu and Yishu Zhai
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- 2022
6. Genome-wide genetic diversity and IBD analysis reveals historic dissemination routes of pear in China
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Xuening Chen, Mingyue Zhang, Manyi Sun, Yueyuan Liu, Shengnan Li, Bobo Song, Mengyan Li, Shaoling Zhang, Runze Wang, Jiaming Li, Kejiao Zhao, and Jun Wu
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Genetics ,Forestry ,Horticulture ,Molecular Biology - Published
- 2021
7. Research on the application of big data in the reform of college education mode—taking sports as an example
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Yueyuan Liu, Rui Li, Wei Zeng, and Zhi Zeng
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business.industry ,Big data ,ComputingMilieux_COMPUTERSANDEDUCATION ,Mathematics education ,Educational evaluation ,business ,Set (psychology) ,Psychology ,Objectivity (science) ,Field (computer science) ,Test (assessment) ,Physical education ,Variety (cybernetics) - Abstract
In recent years, the reform and application of big data in the field of education is booming, and has become an important backing force for educational decision-making, teaching mode optimization and education evaluation reform. Big data can promote teaching and learning, promote the scientific nature of educational decision-making, improve the education quality monitoring system, promote the comprehensiveness and objectivity of education evaluation, and help intelligent education. This paper takes sports as an example to study the application of big data in the reform of college education mode. First of all, through the K-means clustering analysis of more than 90000 college students' physical health data collected in recent three years, according to the analysis results, we set up a variety of directional courses to change the students' physical defects. Then according to the one-year course teaching, through the "experiment feedback experiment" cycle mode, the new teaching mode will be constantly improved. Finally, we use t test to evaluate the teaching effect from three aspects. The results show that students' physical health level has been significantly and comprehensively improved.
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- 2021
8. Involvement of Populus CLEL peptides in root development
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Dongdong Tian, Xueping Shi, Bo Zheng, Lidan Tian, Yueyuan Liu, and Mengjie Wan
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0106 biological sciences ,0301 basic medicine ,Populus trichocarpa ,Signal peptide ,Physiology ,In silico ,Arabidopsis ,Peptide ,Plant Science ,Plant Roots ,01 natural sciences ,03 medical and health sciences ,Protein structure ,Gene Expression Regulation, Plant ,Gene ,Lateral root formation ,chemistry.chemical_classification ,biology ,Arabidopsis Proteins ,fungi ,Lateral root ,biology.organism_classification ,Populus ,030104 developmental biology ,Biochemistry ,chemistry ,Peptides ,010606 plant biology & botany - Abstract
As one of the major groups of small post-translationally modified peptides, the CLV3/EMBRYO SURROUNDING REGION-RELATED (CLE)-like (CLEL) peptide family has been reported to regulate root growth, lateral root development and plant gravitropic responses in Arabidopsis thaliana. In this study, we identified 12 CLEL genes in Populus trichocarpa and performed a comprehensive bioinformatics analysis on these genes. Among them, five P. trichocarpa CLELs (PtrCLELs) were revised with new gene models. All of these PtrCLEL proteins were structurally similar to the A. thaliana CLELs (AtCLELs), including an N-terminal signal peptide, a conserved C-terminal 13-amino-acid CLEL motif and a variable intermediate region. In silico and quantitative real-time PCR analyses showed that PtrCLELs were widely expressed in various tissues, including roots, leaves, buds and stems. Exogenous application of chemically synthesized PtrCLEL peptides resulted in wavy or curly roots and reduced lateral root formation in A. thaliana. Moreover, germinating Populus deltoides seedlings on a growth medium containing these peptides caused the roots to thicken and to form abnormal lateral roots, in many cases in clusters. Anatomical and histological changes in thickened roots were further investigated by treating Populus 717 cuttings with the PtrCLEL10 peptide. We observed that root thickening was mainly due to an increased number of cells in the epidermis, hypodermis and cortex. The results of our study suggested that PtrCLEL and AtCLEL genes encode proteins with similar protein structures, sequences of peptide motif and peptide activities on developing roots. The activities of PtrCLEL peptides in root development were species-dependent.
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- 2019
9. Alternative splicing analysis provides insight into the domestication and improvement of pear (Pyrus pyrifolia) fruit
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Mengyan Li, Runze Wang, Bobo Song, Beibei Cao, Manyi Sun, Yueyuan Liu, Xuening Chen, Shaoling Zhang, Jiaming Li, Kejiao Zhao, and Jun Wu
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Horticulture - Published
- 2022
10. Genome-wide association studies provide insights into the genetic determination of fruit traits of pear
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Yueyuan Liu, Xiaolong Li, Cheng Zou, Yang Guangyan, Mengfan Qin, Manyi Sun, Zhangjun Fei, Shaoling Zhang, Chao Gu, Jiaming Li, Runze Wang, Mingyue Zhang, Hongju Hu, Jun Wu, Cheng Xue, Shutian Tao, Bing Bai, Jing Fan, Shan Wu, Xue Yongsong, and Xu Chen
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Agricultural genetics ,0106 biological sciences ,0301 basic medicine ,Candidate gene ,Science ,Quantitative Trait Loci ,Population ,Arabidopsis ,General Physics and Astronomy ,Genome-wide association study ,Biology ,Genes, Plant ,Lignin ,Genome-wide association studies ,01 natural sciences ,Article ,Plant breeding ,General Biochemistry, Genetics and Molecular Biology ,Pyrus ,03 medical and health sciences ,Genetic variation ,education ,Phylogeny ,Genetic association ,Molecular breeding ,PEAR ,education.field_of_study ,Multidisciplinary ,Genetic Variation ,Reproducibility of Results ,food and beverages ,General Chemistry ,Plants, Genetically Modified ,body regions ,Genetics, Population ,030104 developmental biology ,Evolutionary biology ,Fruit ,Fruit tree ,Genome-Wide Association Study ,010606 plant biology & botany - Abstract
Pear is a major fruit tree crop distributed worldwide, yet its breeding is a very time-consuming process. To facilitate molecular breeding and gene identification, here we have performed genome-wide association studies (GWAS) on eleven fruit traits. We identify 37 loci associated with eight fruit quality traits and five loci associated with three fruit phenological traits. Scans for selective sweeps indicate that traits including fruit stone cell content, organic acid and sugar contents might have been under continuous selection during breeding improvement. One candidate gene, PbrSTONE, identified in GWAS, has been functionally verified to be involved in the regulation of stone cell formation, one of the most important fruit quality traits in pear. Our study provides insights into the complex fruit related biology and identifies genes controlling important traits in pear through GWAS, which extends the genetic resources and basis for facilitating molecular breeding in perennial trees., Studies of fruit quality traits in pears are lagging behind the other major fruit trees. Here, the authors conduct GWAS of fruit quality and phenological traits in a panel of 312 sand pear accessions using SNPs called from resequencing data, and reveal the involvement of a lignin formation-related protein in regulating stone cell development.
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- 2021
11. SVGAN: Semi-supervised Generative Adversarial Network for Image Captioning
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Wei Zeng, Gangqiang He, Yueyuan Liu, and Yi Zhang
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Closed captioning ,Graph embedding ,Computer science ,business.industry ,Detector ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Image (mathematics) ,Task (project management) ,Visualization ,Range (mathematics) ,Task analysis ,Computer vision ,Artificial intelligence ,business - Abstract
Image captioning is a task that enables computer to naturally describe the contents of an image like a human, moreover it involves two different major research fields of computer vision and natural language processing. In this paper, a new image captioning system is proposed, which can address the challenges of automatically describing images in the wild. Built on the state-of-the-art caption framework, we designed a deep visual detector to catch a broad range of visual concepts, a GAN(Generative Adversarial Network) with graph embedding is developed to generate accurate sentences for wild images.
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- 2020
12. Image Processing and Return System Based on ZYNQ
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Yueyuan Liu, Sen Yu, Wei Zeng, and Jingna Guo
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Ethernet ,Computer science ,business.industry ,Gigabit Ethernet ,020206 networking & telecommunications ,Image processing ,Throughput ,02 engineering and technology ,Protocol stack ,Software ,Scalability ,0202 electrical engineering, electronic engineering, information engineering ,020201 artificial intelligence & image processing ,business ,Computer hardware ,Data transmission - Abstract
To transfer the load of processing image data to embedded devices, a solution based on ZYNQ image processing and return system is presented. The platform achieves communication with the host computer by building the LwIP protocol stack, uses ZYNQ hardware and software co-design, and interacts with data through DMA transmission mechanism. The host computer sends image data to ZYNQ via Gigabit Ethernet for processing. The image processing can not only be displayed in real time on this platform, but also be returned to the host computer using Gigabit Ethernet. Experiments show that the design meets the requirements of high-resolution image data with large quantity and high data transmission rate.
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- 2020
13. Contrasting genetic variation and positive selection followed the divergence of NBS-encoding genes in Asian and European pears
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Jugpreet Singh, Runze Wang, Yueyuan Liu, Jiaming Li, Awais Khan, Manyi Sun, Mengfan Qin, Mingyue Zhang, Bobo Song, Zikai Tang, and Jun Wu
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Expansion ,0106 biological sciences ,lcsh:QH426-470 ,lcsh:Biotechnology ,Polymorphism, Single Nucleotide ,01 natural sciences ,Nucleotide diversity ,Domestication ,Evolution, Molecular ,Pyrus ,03 medical and health sciences ,NBS ,lcsh:TP248.13-248.65 ,Genetic variation ,Genetics ,Gene family ,030304 developmental biology ,0303 health sciences ,PEAR ,Genetic diversity ,biology ,food and beverages ,Alternaria ,biology.organism_classification ,Positive selection ,body regions ,lcsh:Genetics ,Fire blight ,Pear ,Research Article ,010606 plant biology & botany ,Biotechnology ,Black spot ,Pyrus communis - Abstract
BackgroundThe NBS disease-related gene family coordinates the inherent immune system in plants in response to pathogen infections. Previous studies have identified NBS-encoding genes inPyrus bretschneideri(‘Dangshansuli’, an Asian pear) andPyrus communis(‘Bartlett’, a European pear) genomes, but the patterns of genetic variation and selection pressure on these genes during pear domestication have remained unsolved.ResultsIn this study, 338 and 412 NBS-encoding genes were identified from Asian and European pear genomes. This difference between the two pear species was the result of proximal duplications. About 15.79% orthologous gene pairs had Ka/Ks ratio more than one, indicating two pear species undergo strong positive selection after the divergence of Asian and European pear. We identified 21 and 15 NBS-encoding genes under fire blight and black spot disease-related QTL, respectively, suggesting their importance in disease resistance. Domestication caused decreased nucleotide diversity across NBS genes in Asian cultivars (cultivated 6.23E-03; wild 6.47E-03), but opposite trend (cultivated 6.48E-03; wild 5.91E-03) appeared in European pears. Many NBS-encoding coding regions showed Ka/Ks ratio of greater than 1, indicating the role of positive selection in shaping diversity of NBS-encoding genes in pear. Furthermore, we detected 295 and 122 significantly different SNPs between wild and domesticated accessions in Asian and European pear populations. Two NBS genes (Pbr025269.1andPbr019876.1) with significantly different SNPs showed >5x upregulation between wild and cultivated pear accessions, and > 2x upregulation inPyrus calleryanaafter inoculation withAlternaria alternata. We propose that positively selected and significantly different SNPs of an NBS-encoding gene (Pbr025269.1) regulate gene expression differences in the wild and cultivated groups, which may affect resistance in pear againstA. alternata.ConclusionProximal duplication mainly led to the different number of NBS-encoding genes inP. bretschneideriandP. communisgenomes. The patterns of genetic diversity and positive selection pressure differed between Asian and European pear populations, most likely due to their independent domestication events. This analysis helps us understand the evolution, diversity, and selection pressure in the NBS-encoding gene family in Asian and European populations, and provides opportunities to study mechanisms of disease resistance in pear.
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- 2020
14. Comparison of multiple algorithms to reliably detect structural variants in pears
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Yueyuan Liu, Jun Wu, Jieying Sun, Shaoling Zhang, Mingyue Zhang, Wenjing Chang, and Manyi Sun
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0106 biological sciences ,lcsh:QH426-470 ,SV detection ,lcsh:Biotechnology ,Pipeline (computing) ,Sequencing data ,Biology ,01 natural sciences ,Deep sequencing ,Pyrus ,03 medical and health sciences ,Software ,Accuracy of SVs ,lcsh:TP248.13-248.65 ,Genetics ,Long-read sequencing ,030304 developmental biology ,0303 health sciences ,Multi-core processor ,business.industry ,Genetic Variation ,High-Throughput Nucleotide Sequencing ,Sequence Analysis, DNA ,Software package ,lcsh:Genetics ,NGS ,Sequencing depth ,business ,Algorithm ,Algorithms ,Genome, Plant ,Research Article ,SV calling pipeline ,010606 plant biology & botany ,Biotechnology - Abstract
Background Structural variations (SVs) have been reported to play an important role in genetic diversity and trait regulation. Many computer algorithms detecting SVs have recently been developed, but the use of multiple algorithms to detect high-confidence SVs has not been studied. The most suitable sequencing depth for detecting SVs in pear is also not known. Results In this study, a pipeline to detect SVs using next-generation and long-read sequencing data was constructed. The performances of seven types of SV detection software using next-generation sequencing (NGS) data and two types of software using long-read sequencing data (SVIM and Sniffles), which are based on different algorithms, were compared. Of the nine software packages evaluated, SVIM identified the most SVs, and Sniffles detected SVs with the highest accuracy (> 90%). When the results from multiple SV detection tools were combined, the SVs identified by both MetaSV and IMR/DENOM, which use NGS data, were more accurate than those identified by both SVIM and Sniffles, with mean accuracies of 98.7 and 96.5%, respectively. The software packages using long-read sequencing data required fewer CPU cores and less memory and ran faster than those using NGS data. In addition, according to the performances of assembly-based algorithms using NGS data, we found that a sequencing depth of 50× is appropriate for detecting SVs in the pear genome. Conclusion This study provides strong evidence that more than one SV detection software package, each based on a different algorithm, should be used to detect SVs with higher confidence, and that long-read sequencing data are better than NGS data for SV detection. The SV detection pipeline that we have established will facilitate the study of diversity in other crops.
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- 2020
15. MOESM8 of Comparison of multiple algorithms to reliably detect structural variants in pears
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Yueyuan Liu, Mingyue Zhang, Jieying Sun, Wenjing Chang, Manyi Sun, Shaoling Zhang, and Wu, Jun
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Additional file 8. Verification of SVs in ‘Yali’ through comparisons with the ‘Dangshansuli’ reference genome.
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- 2020
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16. MOESM3 of Comparison of multiple algorithms to reliably detect structural variants in pears
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Yueyuan Liu, Mingyue Zhang, Jieying Sun, Wenjing Chang, Manyi Sun, Shaoling Zhang, and Wu, Jun
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Additional file 3: Figure S1. The number of genes within SVs detected by software packages using NGS data (a) and long-read sequencing data (b).
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- 2020
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17. Additional file 1 of Contrasting genetic variation and positive selection followed the divergence of NBS-encoding genes in Asian and European pears
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Manyi Sun, Mingyue Zhang, Jugpreet Singh, Song, Bobo, Zikai Tang, Yueyuan Liu, Runze Wang, Mengfan Qin, Jiaming Li, Awais Khan, and Wu, Jun
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Additional file 1: Figure S1. A phylogenetic tree of P. bretschneideri NBS proteins constructed by ML (Maximum likelihood) method using IQ-TREE. Fig. S2: A phylogenetic tree of P. communis NBS proteins constructed by ML method using IQ-TREE. ① Two subfamilies are shown. Red represents non-TIR group and Blue represents TIR group. ② The six classes of NBS-encoding genes are marked by different colors. Green means CC-NBS-LRR type, light yellow means TIR-NBS-LRR type, yellow means NBS type, light blue means TIR-NBS type, orange means CC-NBS type and purple means NBS-LRR type. ③ Domains of NB-ARC, LRR, and TIR are displayed on the tree (CC domain was not shown). Yellow means TIR domain, red means NB-ARC domain and blue means LRR domain.
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- 2020
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18. Dual-modality UDV-PIV system for measurement of solid-liquid flow in sewage facilities
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Yousef Faraj, Tian Mengyuan, Yueyuan Liu, Shaochong Wang, Zihui Wei, Zhao Jixun, and Lide Fang
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Reverberation ,System of measurement ,Acoustics ,Flow (psychology) ,Velocimetry ,Computer Science Applications ,symbols.namesake ,Flow conditions ,Particle image velocimetry ,Approximation error ,Modeling and Simulation ,symbols ,Environmental science ,Electrical and Electronic Engineering ,Instrumentation ,Doppler effect - Abstract
Population growth and global industrialization cause a dramatic increase in the amount of sewage sludge produced annually worldwide from Municipal and Industrial Wastewater treatment. The efficient measurement of sewage, which is a typical solid-liquid two-phase flow, has become an important issue that requires to be urgently addressed. In this study, an improved Ultrasonic Doppler Velocimetry (UDV) is proposed to optimize the probe design and hardware design, which reduces the influence of working frequency and echo reverberation on accuracy and improves the stability of the system. A Doppler peak extraction and superposition method is also put forward to correct the offset of Doppler peak frequency. In this paper, Particle Image Velocimetry (PIV) is used to calibrate the UDV system to modify the measurement model of ultrasonic Doppler liquid-solid two-phase flow, and dynamic experiments are carried out in a vertical steel pipe with inner diameter of 50 mm at different flow conditions. The results show that the accuracy and stability of UDV measurement system are greatly improved, with a maximum relative error of 1.49%.
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- 2021
19. Identification of key genes related to seedlessness by genome-wide detection of structural variation and transcriptome analysis in ‘Shijiwuhe’ pear
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Jun Wu, Shaoling Zhang, Jintao Xu, Qingyu Li, Runze Wang, Jieying Sun, Yueyuan Liu, Yang Guangyan, Wen-Quan Le, Mingyue Zhang, Baofeng Hao, and Yuanjun Li
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0301 basic medicine ,China ,Candidate gene ,Population ,RNA-Seq ,Biology ,Parthenocarpy ,Polymorphism, Single Nucleotide ,Pyrus ,Transcriptome ,Structural variation ,03 medical and health sciences ,0302 clinical medicine ,Gene Expression Regulation, Plant ,Genetics ,education ,PEAR ,education.field_of_study ,Gene Expression Profiling ,fungi ,Chromosome Mapping ,food and beverages ,General Medicine ,WRKY protein domain ,030104 developmental biology ,Fruit ,030220 oncology & carcinogenesis ,Seeds ,Genome-Wide Association Study - Abstract
Seedless fruits are highly marketable because they are easier to eat than fruits with seeds. ‘Shijiwuhe’ is a seedless pear cultivar that is a mutant derived from an F1 hybridization population (‘Bartlett’ x ‘Yali’). Little is known about the key genes controlling seedless pear fruit. In this study, field experiments revealed that seedless ‘Shijiwuhe’ pear was not due to parthenocarpy, and that it was self-incompatible. Single nucleotide polymorphisms (SNPs), small insertions and deletions (InDels) and structural variations (SVs) were characterized using DNA sequencing data between ‘Shijiwuhe’ and parental cultivars. A total of 1498 genes were found to be affected by SV and over 50% of SVs were located in promoter regions. Transcriptome analysis was conducted at three time points (4, 8, and 12 days after cross-pollination) during early fruit development of ‘Shijiwuhe’, ‘Bartlett’, and ‘Yali’. In total, 1438 differentially expressed genes (DEGs) were found between ‘Shijiwuhe’ and parental cultivars ‘Bartlett’ and ‘Yali’. We found 1193 SVs that caused differential expression of genes at 4 DACP. Among them, over 100 genes were in pathways related to seed nutrition and energy storage and 41 candidate genes encoded several important transcription factors, such as MYB, WRKY, NAC, and bHLH, which might play important roles in seed development. The qRT-PCR results also confirmed that the candidate genes with SVs showed differential expression between ‘Shijiwuhe’ pear and ‘Bartlett’ or ‘Yali’. This study, which combined field experiments, SV detection, and transcriptome analysis might provide an effective way to predict the candidate genes regulating the seedless trait and important gene resources for genetic improvement of pear.
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- 2020
20. The evaluation of recombinant hookworm antigens as vaccines in hamsters (Mesocricetus auratus) challenged with human hookworm, Necator americanus
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Angela L. Williamson, Peter J. Hotez, Shu-Hua Xiao, Gaddam Goud, Jian Xue, Sen Liu, Yueyuan Liu, Bin Zhan, Alex Loukas, and Vehid Deumic
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Male ,Necator americanus ,medicine.medical_treatment ,Molecular Sequence Data ,Immunology ,Hamster ,Necatoriasis ,Open Reading Frames ,Antigen ,Cricetinae ,parasitic diseases ,medicine ,Animals ,Humans ,Amino Acid Sequence ,Cloning, Molecular ,Hookworm infection ,Vaccines, Synthetic ,Hookworm vaccine ,Base Sequence ,Mesocricetus ,biology ,fungi ,General Medicine ,biology.organism_classification ,Virology ,Recombinant Proteins ,Cysteine Endopeptidases ,Disease Models, Animal ,Infectious Diseases ,Antigens, Helminth ,Larva ,Antigens, Surface ,Parasitology ,Ancylostoma caninum ,Sequence Alignment ,Adjuvant ,medicine.drug - Abstract
We have previously reported the successful adaptation of human hookworm Necator americanus in the golden hamster, Mesocricetus auratus. This animal model was used to test a battery of hookworm (N. americanus and Ancylostoma caninum) recombinant antigens as potential vaccine antigens. Hamsters immunized a leading vaccine candidate N. americanus-Ancylostoma secreted protein 2 (Na-ASP-2) and challenged with N. americanus infective larvae (L3), resulted in 30-46.2% worm reduction over the course of three vaccine trials, relative to adjuvant controls. In addition, significant reduction of worm burdens was also observed in the hamsters immunized with adult hookworm antigens A. caninum aspartic protease 1 (Ac-APR-1); A. caninum-glutathione-S transferase 1 (Ac-GST-1) and Necator cysteine proteases 2 (Na-CP-2) (44.4%, 50.6%, and 29.3%, respectively). Our data on the worm burden reductions afforded by these hookworm antigens approximate the level of protection reported previously from dogs challenged with A. caninum L3, and provide additional evidence to support these hookworm antigens as vaccine candidates for human hookworm infection. The hamster model of N. americanus provides useful information for the selection of antigens to be tested in downstream vaccine development.
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- 2008
21. Molecular cloning and characterization of Ac-MTP-2, an astacin-like metalloprotease released by adult Ancylostoma caninum☆
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Jianjun Feng, Sen Liu, Bin Zhan, Angela L. Williamson, Alex Loukas, Peter J. Hotez, Yueyuan Liu, and Gaddam Goud
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Ancylostoma ,DNA, Complementary ,Molecular Sequence Data ,Fluorescent Antibody Technique ,Complementary DNA ,Immunoscreening ,Animals ,Amino Acid Sequence ,Cloning, Molecular ,Molecular Biology ,Peptide sequence ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,cDNA library ,Metalloendopeptidases ,Helminth Proteins ,biology.organism_classification ,Molecular biology ,Recombinant Proteins ,Open reading frame ,Secretory protein ,Metalloproteases ,Parasitology ,Ancylostoma caninum ,Astacin ,Sequence Alignment - Abstract
Ac-MTP-2 is an astacin-like metalloprotease secreted by adult Ancylostoma caninum hookworms. Ac-mtp-2 cDNA was cloned by immunoscreening a cDNA library with antisera prepared against adult A. caninum excretory/secretory (ES) products. The full-length Ac-mtp-2 contains 850 bp cDNA encoding a 233 amino acid open reading frame (ORF) with 32% amino acid identity to Ce-NSP-4, a pharyngeal cell-derived secreted metalloprotease of the nematode Caenorhabditis elegans. The predicted ORF contained a conserved Met-turn sequence (SXMHY), but only a partial zinc-binding signature sequence (GXXXEHXRXER instead of HEXXHXXGXXHEXXRXDR) found in other astacins. However, by both gelatin gel electrophoresis and azocasein digestion, the recombinant Ac-MTP-2 exhibited proteolytic activity that was inhibited by the zinc chelator 1,10-phenanthroline and Ac-TMP, a putative tissue inhibitor of metalloprotease that was previously shown to be a highly abundant component of adult A. caninum ES products. By RT-PCR, Western blot Ac-MTP-2 was found only expressed in adult hookworms and secreted in the adult ES products. Immunolocalization with antisera shows that Ac-MTP-2 is located to the esophageal glands (confirming its role as a secretory protein), as well as to the parasite uterus. It is hypothesized that Ac-MTP-2 functions in the extracorporeal digestion of the intestinal mucosal plug lodged in the buccal capsule of the adult parasite.
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- 2007
22. Cloning, Yeast Expression, Isolation, and Vaccine Testing of RecombinantAncylostoma‐Secreted Protein (ASP)–1 and ASP‐2 fromAncylostoma ceylanicum
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Bin Zhan, Sophia Chung-Debose, Alex Loukas, Sen Liu, Gaddam Goud, Vehid Deumic, Reshad Dobardzic, Susana Mendez, Kashinath Ghosh, Laura Hoffman, Rachna Patel, Yueyuan Liu, Peter J. Hotez, Azra Dobardzic, Qun Jin, Bernard C. Zook, and John M. Hawdon
- Subjects
Ancylostoma ,DNA, Complementary ,Molecular Sequence Data ,Hamster ,Saccharomyces cerevisiae ,law.invention ,Pichia pastoris ,law ,parasitic diseases ,medicine ,Animals ,Immunology and Allergy ,Amino Acid Sequence ,Cloning, Molecular ,Conserved Sequence ,DNA Primers ,Ancylostoma ceylanicum ,Hookworm vaccine ,Base Sequence ,Sequence Homology, Amino Acid ,biology ,Antibody titer ,Helminth Proteins ,biology.organism_classification ,Fusion protein ,Virology ,Infectious Diseases ,Larva ,Recombinant DNA ,Sequence Alignment ,medicine.drug - Abstract
cDNAs encoding 2 Ancylostoma-secreted proteins (ASPs), Ancylostoma ceylanicum (Ay)-ASP-1 and Ay-ASP-2, were cloned from infective third-stage larvae (L3) of the hookworm A. ceylanicum and were expressed as soluble recombinant fusion proteins secreted by the yeast Pichia pastoris. The recombinant fusion proteins were purified, adjuvant formulated, and injected intramuscularly into hamsters. Hamsters vaccinated either by oral vaccination with irradiated L3 (irL3) or by injections of the adjuvants alone served as positive and negative controls, respectively. Anti-ASP-1 and anti-ASP-2 antibody titers exceeded 1 : 100000. Each vaccinated hamster was challenged orally with 100 L3. Two groups of vaccinated hamsters (i.e., those vaccinated with either irL3 or ASP-2 formulated with Quil A) exhibited significant reductions in adult hookworm burdens, compared with control hamsters. The hookworms recovered from the hamsters vaccinated with ASP-2 plus Quil A were reduced in length. Splenomegaly, which was observed in control hamsters, was not seen in hamsters vaccinated with either irL3 or ASP-2 formulated with Quil A. These results indicate that ASP-2 is a promising molecule for the development of a hookworm vaccine.
- Published
- 2004
23. Case Study of Multi-literacy Ability of English Teachers—An Example of Tianjin Open University
- Author
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Junhong Gao, Xingcai Zhang, and Yueyuan Liu
- Subjects
Engineering ,Linguistic diversity ,business.industry ,media_common.quotation_subject ,Digital native ,ComputingMilieux_COMPUTERSANDEDUCATION ,Mathematics education ,Open university ,Critical awareness ,Variety (linguistics) ,business ,Literacy ,media_common - Abstract
Numerous attention has been shifted to multiliteracies approaches which prepare students for better achievements and fulfilling employment in their future life characterized by the increasing cultural and linguistic diversity as well as the variety in communication channels supported by high technologies. The traditional literacy pedagogy which focuses on the ability to read and write in formal language is generally discarded since it is unsuitable for the "digital natives" who are born in the digital age and is virtually impossible to equip students with critical awareness to handle social practices in societies. Teaching approaches in classrooms are largely dependent on teachers who are the organizers and conductors of instruction practices. The author of this paper conducts a case study in one of the open universities in Tianjin, China, to investigate whether EFL teachers are competent enough to adopt multiliteracies approaches and how well EFL teachers in China are informed of the multiliteracies approaches. Teachers' teaching materials including PPT and other resources are analyzed and interviews are conducted to elicit feedback from EFL teachers towards multiliteracies approaches. Results show that sufficient teacher training is necessary to better prepare EFL teachers to implement multiliteracies approaches.
- Published
- 2014
24. Biochemical characterization and vaccine potential of a heme-binding glutathione transferase from the adult hookworm Ancylostoma caninum
- Author
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Sen Liu, John M. Hawdon, Bin Zhan, Peter J. Hotez, Alex Loukas, Samirah Perally, Susana Mendez, Jeffrey M. Bethony, Shu-Hua Xiao, Gaddam Goud, Jianjun Feng, Karen Jones, Angela L. Williamson, Yan Wang, Ricardo Toshio Fujiwara, Jian Xue, Peter M. Brophy, Yueyuan Liu, and Lilian Lacerda Bueno
- Subjects
Hemeproteins ,Ancylostoma ,Heme binding ,Immunology ,Molecular Sequence Data ,Heterologous ,Microbiology ,Necator americanus ,Ancylostomiasis ,chemistry.chemical_compound ,Heme-Binding Proteins ,Dogs ,Cricetinae ,parasitic diseases ,medicine ,Parasite hosting ,Animals ,Amino Acid Sequence ,RNA, Messenger ,Cloning, Molecular ,Heme ,Glutathione Transferase ,Hookworm vaccine ,Vaccines ,biology ,Glutathione ,biology.organism_classification ,Molecular biology ,Infectious Diseases ,chemistry ,Larva ,Microbial Immunity and Vaccines ,Parasitology ,Ancylostoma caninum ,Carrier Proteins ,medicine.drug - Abstract
We report the cloning and expression of Ac -GST-1, a novel glutathione S -transferase from the adult hookworm Ancylostoma caninum , and its possible role in parasite blood feeding and as a vaccine target. The predicted Ac -GST-1 open reading frame contains 207 amino acids (mass, 24 kDa) and exhibited up to 65% amino acid identity with other nematode GSTs. mRNA encoding Ac -GST-1 was detected in adults, eggs, and larval stages, but the protein was detected only in adult hookworm somatic extracts and excretory/secretory products. Using antiserum to the recombinant protein, Ac -GST-1 was immunolocalized to the parasite hypodermis and muscle tissue and weakly to the intestine. Recombinant Ac -GST-1 was enzymatically active, as determined by conjugation of glutathione to a model substrate, and exhibited a novel high-affinity binding site for hematin. The possible role of Ac -GST-1 in parasite heme detoxification during hemoglobin digestion or heme uptake prompted interest in evaluating it as a potential vaccine antigen. Vaccination of dogs with Ac -GST-1 resulted in a 39.4% reduction in the mean worm burden and 32.3% reduction in egg counts compared to control dogs following larval challenge, although the reductions were not statistically significant. However, hamsters vaccinated with Ac -GST-1 exhibited statistically significant worm reduction (53.7%) following challenge with heterologous Necator americanus larvae. These studies suggest that Ac -GST-1 is a possible drug and vaccine target for hookworm infection.
- Published
- 2005
25. Effect of combining the larval antigens Ancylostoma secreted protein 2 (ASP-2) and metalloprotease 1 (MTP-1) in protecting hamsters against hookworm infection and disease caused by Ancylostoma ceylanicum
- Author
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Vehid Deumic, Bin Zhan, Sen Liu, Jeffrey M. Bethony, Yueyuan Liu, Wenhui Wu, Susana Mendez, Gaddam Goud, Kashinath Ghosh, Reshad Dobardzic, Azra Dobardzic, and Peter J. Hotez
- Subjects
Ancylostoma ,Molecular Sequence Data ,Antibodies, Helminth ,Biology ,Ancylostomiasis ,Feces ,Hemoglobins ,Hookworm Infections ,Antigen ,Cricetinae ,medicine ,Animals ,Amino Acid Sequence ,Cloning, Molecular ,Parasite Egg Count ,Ancylostoma ceylanicum ,Hookworm vaccine ,Vaccines, Synthetic ,General Veterinary ,General Immunology and Microbiology ,Mesocricetus ,Body Weight ,Public Health, Environmental and Occupational Health ,Antibody titer ,Helminth Proteins ,biology.organism_classification ,medicine.disease ,Virology ,Infectious Diseases ,Antigens, Helminth ,Larva ,Metalloproteases ,Molecular Medicine ,medicine.drug - Abstract
Syrian Golden hamsters were vaccinated with the recombinant fusion proteins Ay-ASP-2 and Ay-MTP-1 from the infective larvae of the hookworm Ancylostoma ceylanicum. Vaccines comprised each antigen alone or the combination of the two proteins. All vaccinated group developed high antibody titers (>1:40,000); coadministration of a second antigen did not significantly affect the magnitude of the antibody response. Following challenge, hamsters vaccinated with each single antigen exhibited reductions in worm burden (32% and 28% to Ay-ASP-2 and Ay-MTP-1, respectively) and fecal egg counts (56% and 43%, respectively). A vaccine cocktail, containing both antigens further reduced worm burden (36%) and fecal egg counts (59%) (p
- Published
- 2004
26. Molecular characterisation of the Ancylostoma-secreted protein family from the adult stage of Ancylostoma caninum
- Author
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Peter J. Hotez, Bin Zhan, Alex Loukas, Mahnaz Badamchian, John M. Hawdon, Jianjun Feng, Yueyuan Liu, and Angela L. Williamson
- Subjects
Ancylostoma ,biology ,Protein family ,Base Sequence ,Reverse Transcriptase Polymerase Chain Reaction ,Immune Sera ,fungi ,Protein domain ,Blotting, Western ,Molecular Sequence Data ,Helminth Proteins ,biology.organism_classification ,Molecular biology ,Immunohistochemistry ,Host-Parasite Interactions ,Infectious Diseases ,Secretory protein ,Excretory system ,parasitic diseases ,Animals ,Parasitology ,Adult stage ,Ancylostoma caninum ,Pathogenesis-related protein - Abstract
The Ancylostoma-secreted proteins are a family of nematode-specific cysteine-rich secreted proteins belonging to the pathogenesis-related protein superfamily. Previously we reported that third stage infective larvae of Ancylostoma caninum produce two different Ancylostoma-secreted proteins, a single and double-domain Ancylostoma-secreted protein, designated as Ancylostoma-secreted protein-1 and Ancylostoma-secreted protein-2, respectively. Here we report that adult A. caninum hookworms produce and release four additional Ancylostoma-secreted proteins (Ancylostoma-secreted protein-3-6). Using antiserum against adult excretory/secretory products, Ancylostoma-secreted protein cDNAs were isolated from cDNA expression libraries. Immunolocalisation experiments using specific antisera indicated that the single-domain Ac-Ancylostoma-secreted protein-3 is located in the adult pharyngeal and oesophageal glands. Ac-Ancylostoma-secreted protein-4, Ancylostoma-secreted protein-5 and Ancylostoma-secreted protein-6 are composed of two pathogenesis-related protein domains linked in tandem as a heterodimorphic repeat. Ac-Ancylostoma-secreted protein-4 is localised to the cuticular surface of the adult hookworm, whereas Ac-Ancylostoma-secreted protein-5 was found in the intestinal brush border membrane, and Ancylostoma-secreted protein-6 in the cephalic and excretory glands. All of the adult Ancylostoma-secreted proteins were identified in excretory/secretory products of adult hookworms by Western blotting and are presumably released by the parasite. None of the adult Ancylostoma-secreted proteins were detected by immunoblotting in L3 extracts, although mRNAs of Ac-Ancylostoma-secreted protein-3 and Ac-Ancylostoma-secreted protein-4 were present in the larval stage. The functions of the adult Ancylostoma-secreted proteins are unknown, although the secretion of multiple family members by the adult suggests an important role in the establishment or maintenance of the parasitic relationship.
- Published
- 2003
27. Ac-FAR-1, a 20 kDa fatty acid- and retinol-binding protein secreted by adult Ancylostoma caninum hookworms: gene transcription pattern, ligand binding properties and structural characterisation
- Author
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Sridhar V, Basavaraju, Sridhar, Basavaraju, Bin, Zhan, Malcolm W, Kennedy, Yueyuan, Liu, John, Hawdon, and Peter J, Hotez
- Subjects
Ancylostoma ,Transcription, Genetic ,Molecular Sequence Data ,Biology ,Ligands ,law.invention ,Ancylostomiasis ,Dogs ,law ,parasitic diseases ,Animals ,Amino Acid Sequence ,Cloning, Molecular ,Molecular Biology ,Peptide sequence ,Phylogeny ,chemistry.chemical_classification ,Messenger RNA ,Base Sequence ,Fatty Acids ,Fatty acid ,Helminth Proteins ,biology.organism_classification ,Molecular biology ,Recombinant Proteins ,Amino acid ,Retinol-Binding Proteins ,Open reading frame ,Retinol binding protein ,Biochemistry ,chemistry ,Recombinant DNA ,Parasitology ,Ancylostoma caninum ,Carrier Proteins ,Sequence Alignment - Abstract
Antibody against adult Ancylostoma caninum excretory-secretory (ES) products was used to immunoscreen a cDNA expression library leading to the isolation of cDNAs encoding putative hookworm fatty-acid and retinol-binding proteins. Ac-far-1 and Ac-far-2 cDNAs encode open reading frames corresponding to approximately 20kDa proteins with 91 percent amino acid identity. Ac-FAR-1 and Ac-FAR-2 exhibit clear similarities to other FARs of parasitic nematodes, most closely to two of the FAR proteins of Caenorhabditis elegans (Ce-FAR-1 and Ce-FAR-2). By reverse transcriptase polymerase chain reaction (RT-PCR) assay, Ac-far-1 mRNA was detected in both adult and third-stage larvae of A. caninum. However, the respective proteins were detectable by immunoblot only in adult hookworm ES products and adult extracts. Using fluorescence-based binding assays, bacterial recombinant Ac-FAR-1 was found to bind fatty acids and retinol (Vitamin A) with dissociation constants in the micromolar region. Circular dichroism spectra indicated that Ac-FAR-1 possesses a high level of alpha-helix, similar to Ov-FAR-1 from Onchocerca volvulus. This is the first demonstration of a functional FAR secreted by adult hookworms and provides further evidence that FAR proteins secreted by parasitic nematodes are crucial to parasitism.
- Published
- 2003
28. Corrigendum to 'Ac-FAR-1, a 20 kDa fatty acid- and retinol-binding protein secreted by adult Ancylostoma caninum hookworms: Gene transcription pattern, ligand binding properties and structural characterization' [Mol. Biochem. Parasitol. 126 (1) (2003) 63–71]
- Author
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Yueyuan Liu, Sridhar V. Basavaraju, Bin Zhan, John M. Hawdon, Malcolm W. Kennedy, and Peter J. Hotez
- Subjects
chemistry.chemical_classification ,Retinol binding protein ,Biochemistry ,biology ,chemistry ,Mole ,Fatty acid ,Parasitology ,Ancylostoma caninum ,biology.organism_classification ,Molecular Biology ,Molecular biology - Published
- 2010
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