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Industrial advancement has led to an increase in the production and usage of bisphenol A (BPA), thereby resulting in serious environmental pollution problems. BPA ingestion causes multiorgan toxicity. However, the exact mechanism underlying BPA-induced colon damage remains elusive. Moreover, no safe treatment is available to alleviate BPA-induced colon injury. Therefore, the in vivo and in vitro approaches were employed to detect the protective effects of melatonin (MT) on BPA-induced colon injury and to determine the underpinning molecular mechanisms. MT treatment of mice and the colonic epithelial cells NCM460 alleviated BPA-induced colon damage by inhibiting the mitochondrial dynamic imbalance, enhancing mitochondrial respiratory chain (MRC) complexes expression, reducing reactive oxygen species (ROS) production, and suppressing apoptosis and necroptosis. MT upregulated the proteins level of silent information regulator 1 (SIRT1) and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), which further increased the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and the downstream antioxidant target genes heme oxygenase-1 (HO-1) and NAD(P)H quinone redox enzyme-1 (NQO1). Treatment with the SIRT1 inhibitor EX527 effectively reversed the MT-induced upregulation of the aforementioned protein levels. Thus, the MT-activated Sirt1/PGC-1α signaling pathway restored the mitochondrial dynamic balance and activated the Nrf2 antioxidant axis to attenuate BPA-induced colon injury. These results demonstrate that MT supplementation may potentially mitigate BPA toxicity.

Nanoplastics (NPs) existing in aquatic ecosystem is an emerging environmental pollutant, which has become a nagging serious environmental problem. Miniaturized plastic fragments with different diameters have different penetration capabilities to body tissues, and thus may have different toxicity to the target organs. However, the specific toxicological effects and mechanisms of NPs with different particle sizes on aquatic animal hearts are still unknown. To this end, carps were directly exposed to the aqueous environment of polystyrene NPs (1000 μg/L, PS-NPs) with three particle sizes (50 nm, 100 nm and 400 nm), respectively, for 28 days. HE and TUNEL staining displayed that exposed to PS-NPs of three diameters all caused myocardial tissue inflammation and cardiomyocyte apoptosis in carps. Of note, at the same exposure concentration, the damage caused by PS-NPs with particle size of 50 nm was more serious than that of 100 nm and 400 nm. Further research found that, in carp hearts exposed to PS-NPs, the levels of carp innate immunity-related components TLR4 and NOX2 were significantly higher than those in controls and were negatively correlated with the exposed particle size. The content of ROS increased significantly, the activities of antioxidant enzymes (CAT, SOD1 and Gpx1) decreased, and MDA accumulated. In addition, as the particle size of PS-NPs decreased, Th1 cells gradually replaced Th2 cells to dominate, the Th1/Th2 balance was dysregulated, and the expression of apoptosis-inducing pathway IGFBP3/p53/ACHE-related genes was increased, markedly. Overall, our study results demonstrated that PS-NPs exposure caused oxidative stress, resulting in inflammation and apoptosis in carp heart, and the degree of damage was negatively correlated with the particle size of PS-NPs. Our work enriched the theoretical basis for NPs toxicological research and shed new light on the risk of NPs exposure.

Metal-free heteroatom doped nanocarbons are promising alternatives to the metal-based materials in catalytic ozonation for destruction of aqueous organic contaminants. In this study, N, S co-doped hollow carbon microspheres (NSCs) were synthesized from the polymerization products during persulfate wet air oxidation of benzothiazole. The contents of doped N and S as well as the structural stability were maneuvered by adjusting the subsequent N2-annealing temperature. Compared with the prevailing single-walled carbon nanotubes, the N2-annealed NSCs demonstrated a higher catalytic ozonation activity for benzimidazole degradation. According to the quantitative structure-activity relationship (QSAR) analysis, the synergistic effect between the graphitic N and the thiophene-S which redistributed the charge distribution of the carbon basal plane contributed to the activity enhancement of the N2-annealed NSCs. Additionally, the hollow structure within the microspheres served as the microreactor to boost the mass transfer and reaction kinetics via the nanoconfinement effects. Quenching and electron paramagnetic resonance (EPR) tests revealed that benzimidazole degradation was dominated by the produced singlet oxygen (1O2) species, while hydroxyl radicals (•OH) were also generated and participated. This study puts forward a novel strategy for synthesis of heteroatom-doped nanocarbons and sheds a light on the relationship between the active sites on the doped nanocarbons and the catalytic performance.

“Urban renovation and ecological restoration” is an effective means to control “Urban disease”, improve the living environment and change the urban development mode. This article from the perspective of “Urban renovation and ecological restoration” by Nanjing Jinling shipyard development problems, from the ecological restoration, cultural remodeling and function transformation and other respects, discusses the current Jinling shipyard area between industrial heritage protection and cultural inheritance update problem. Using field research, literature reading, case study and other methods, and put forward corresponding planning strategies and suggestions. Integrate the needs of people’s livelihood with urban industrial memory to realize the maximum protection, renewal and utilization of industrial heritage areas.

This paper starts with the research on the value feature quantification and data spatialization of the historical and cultural district of Yuejin Road, Mianyang. Through the intuitive display of the industrial heritage value evaluation results, it can better guide the protection and management of the area. The building value evaluation system and the industrial heritage information database are established through GIS technology. On the basis of determining the 8 first-level indicators, the analytic hierarchy process is used to determine the weight of each factor, and then weighted and superimposed to form a comprehensive value evaluation map. Combined with the analysis of the value evaluation results, a corresponding strategy is proposed for the renovation and renewal of the historical and cultural block of Yuejin Road, and better promotes the development of informatization and scientificization of the protection and renovation of the historical block.

Cadmium (Cd), a common toxic heavy metal, is believed as a risk factor for the induction and progression of cardiovascular disease. Autophagy is a highly ordered intracellular lysosomal-mediated degradation pathway that is crucial for protein and organelle quality control. Autophagy dysfunction could develop exacerbated cardiac dysfunction. However, the role of autophagy in Cd exposure-induced cardiotoxicity remains largely unknown. In this study, the Cd-induced swine cardiotoxicity model was established by feeding with a CdCl

Di(2-ethylhexyl) phthalate (DEHP) is a widely artificial persistent organic pollutant, the contamination of which infiltrates daily human life from many aspects, imperceptibly causing damage to multiple organs in the body, including the liver. Apigenin (APG) is widely distributed in vegetables and fruits and can relieve or prevent the injuries caused by exogenous chemicals through various pharmacological effects, such as antioxidant effects. To investigate the mechanism of DEHP-induced liver injury and the antagonistic effects of APG, we treated AML12 cells with 1 mM DEHP and/or APG. Ultrastructural morphology analysis indicated that DEHP induced typical ferroptosis-like damage. In addition, we found that DEHP exposure induced ferroptosis by enhancing reactive oxygen species (ROS) levels, disrupting iron homeostasis and lipid peroxidation, and regulating the expression of ferroptosis-related genes. Notably, supplementation with APG significantly inhibited these abnormal changes, and molecular docking further showed evidence of the activating effects of APG ligand on glutathione peroxidase 4 (GPX4). These results demonstrated that the protective effects of APG on DEHP-induced ferroptosis were achieved by activating GPX4 and suppressing intracellular iron accumulation. This information not only adds to DEHP toxicological data but also provides a basis for the practical application of APG.

One of the most remarkable functional feats accomplished by visual system is the interpolation of missing retinal inputs based on surrounding information, a process known as perceptual completion. Perceptual completion enables the active construction of coherent, vivid percepts from spatially discontinuous visual information that is prevalent in real-life visual scenes. Despite mounting evidence linking sensory activity enhancement and perceptual completion, surprisingly little is known about whether and how attention, a fundamental modulator of sensory activities, affects perceptual completion. Using EEG-based time-resolved inverted encoding model (IEM), we reconstructed the moment-to-moment representation of the illusory grating that resulted from spatially interpolating the orientation of surrounding inducers. We found that, despite manipulation of observers' attentional focus, the illusory grating representation unfolded in time in a similar manner. Critically, attention to the surrounding inducers simultaneously attenuated the illusory grating representation and delayed its temporal development. Our findings disclosed, for the first time, the suppressive role of selective attention in perceptual completion and were suggestive of a fast, automatic neural machinery that implements the interpolation of missing visual information.

Cadmium (Cd) is the main environmental pollutant causing endocrine and nervous system dysfunction in animals. High doses of Cd cause cytotoxicity, including programmed necrosis and apoptosis, which has aroused widespread concern. Mitochondrial dynamics plays a key role in programmed necrosis and apoptosis of endocrine organs. Nevertheless, there is a lack of information on the relationship between Cd-induced programmed necrosis/apoptosis of the hypothalamus and the mitochondrial fusion-fission balance. Therefore, a hypothalamic injury model of Cd exposure was established by adding 20 mg/kg CdCl

Selenium (Se) is one of the essential trace elements; its deficiency induces ROS production and cell death in cardiomyocytes, skeletal muscle cells, and vascular smooth muscle cells, but it is still not clear the impact of Se deficiency on human uterine smooth muscle cells (HUSMCs). To investigate the effect of low Se on the mRNA expression of selenoproteins, the mRNA and protein expression of apoptosis and necroptosis of HUSMCs and their mechanism, Se deficient HUSMCs mode was established through culturing with 1% FBS containing 0 ng/mL, 0.7 ng/mL, and 7 ng/mL Se, and 10% FBS was as the control group. Then, the apoptosis and necroptosis rates, intracellular ROS content and the expression levels of selenoproteins, apoptosis, necroptosis, MAPK pathway-related genes were examined under different Se concentrations. The results showed that Se deficiency led to the augment of cell apoptosis and necroptosis in HUSMCs (p

Imidacloprid (IMI) is a widely used neonicotinoid insecticide that has toxic effects on nontarget organisms. 1,8-Cineole (eucalyptol) is purified from essential oils in several aromatic plants and can prevent xenobiotic toxicity. The kidney is a major organ for xenobiotic elimination and thus has high risk of exposure. The purpose of this research was to clarify the effect of IMI exposure on autophagy in fish kidney cells, determine the potential of eucalyptol to provide cytoprotection from the toxicity of the neonicotinoid pesticide IMI, and identify its mechanism of action. Therefore, the Ctenopharyngodon idellus kidney cell line (CIK cell) was treated with 20 mg/L IMI and/or 20 μM eucalyptol for 48 h as the research objective. The results showed that IMI exposure induced autophagy accompanied by advanced autophagy markers BNIP3, Beclin1 and LC3Ⅱ/Ⅰ in CIK cells, reduced the levels of miR-451, increased the expression of Cab39 and AMPK, inhibited AKT/mTOR signaling, and activated the JNK pathway. Eucalyptol treatment alleviated IMI-induced autophagy and relieved the activation of autophagy-associated signals. These results indicate that eucalyptol could alleviate IMI-induced autophagy through the miR-451/Cab39/AMPK axis in fish kidney cells. These results partly explained the mechanism of biological threat on fish under IMI exposure and the potential application value of EUC in aquaculture.

To suppress the notorious shuttle effect and capacity fading in lithium-sulfur battery, polyaniline has been introduced due to its multi-anchoring sites for polysulfides, but high content of polyaniline is inevitably accompanied by increase in the inactive content of total cathode and sacrifice in the energy density. Here we disclose that extremely low polyaniline loading can realize excellent cell performance, where conductive polyaniline is uniformly produced on the surface of sulfur-wrapped carbon nanoparticles via dilute aniline polymerization. Even ca. 1 wt% of polyaniline loading is enough to suppress diffusion and dissolution of polysulfides, and the sluggish charge transfer is accelerated. In addition to enhanced cycle stability, a remarkable increase in capacity by ca. 46% is realized compared to sulfur-wrapped carbon composite after 100th at 0.5 C. Moreover, an electrochemical property with coulombic efficiency above 95% and a high capacity retention rate of 55% is achieved even after 500 cycles at ca. 2 wt% polyaniline loading.

Acute kidney injury (AKI) is a frequent and serious complication of sepsis; however, there are currently no effective therapies. Inflammation and oxidative stress are the major mechanisms implicated in lipopolysaccharide (LPS)-induced AKI. Dexmedetomidine (DEX) has been reported to have remarkable anti-inflammatory and antioxidant effects. Here, we examined the renoprotective effects of DEX and potential underlying mechanisms in rats with LPS-induced AKI. We analyzed renal function and structure; serum inflammatory cytokine; renal oxidant and antioxidant levels; and renal expression of glycogen synthase kinase-3β (GSK-3β)/nuclear factor erythroid 2-related factor 2 (Nrf2) pathway-related proteins in rats 4 hr after administration of LPS. Pretreatment with DEX improved renal function and significantly reduced the levels of inflammatory cytokines and oxidative stress markers. Treatment with DEX and the GSK-3β inhibitor SB216367 promoted phosphorylation of GSK-3β, induced Nrf2 nuclear translocation, and increased transcription of the Nrf2 target genes heme oxygenase-1 and NAD(P)H quinone oxidoreductase-1, primarily in renal tubules. Alpha-2-adrenergic receptor (α2-AR) antagonist atipamezole and imidazoline I 2 receptor (I 2 R) antagonist idazoxan reversed the effects of DEX. These results suggest that the renoprotective effects of DEX are mediated via α2-AR and I 2 R-dependent pathways that reduce inflammation and oxidative stress through GSK-3β/Nrf2 signaling.

Cadmium (Cd) is a widely distributed environmental pollutant with immunotoxicity and endocrine toxicity. M1/M2 macrophages participate in the immune response and exert an essential influence on fibrosis. Nevertheless, whether Cd can induce porcineadrenal fibrosis by affecting the polarization of M1/M2 macrophages and its potential regulatory mechanism have not been explored. We added 20 mg/kg CdCl2 to the pig diet for 40 days to investigate the fibrogenic effect of subacute Cd exposure on the adrenal gland. The results indicated that the ACTH and CORT in serum were decreased by 15.26 % and 21.99 %, respectively. The contents of adrenal mineral elements Cd, Cr, Mn were increased up to 34, 1.93, 1.42 folds and Co, Zn, Sn were reduced by 21.57 %, 20.52 %, 15.75 %. Concurrently, the pro-oxidative indicators (LPO, MDA and H2O2) were increased by 1.85, 2.20, 2.77 folds and 3.60, 11.15, 4.11 folds upregulated mRNA levels of TLR4, NF-κB, NLRP3 were observed. Subsequently, the expression of M1 macrophages polarization markers (IL-6, iNOS, TNF-α, CCL2 and CXCL9) were raised by 2.03, 2.30, 2.35, 1.58, 1.56 folds, while M2 macrophages (IL-4, CCL24, Arg1, IL-10, MRC1) showed a 62.34 %, 31.88 %, 50.26 %, 74.00 %, 69.34 % downregulation. The expression levels of AMPK subunits and genes related to glycolysis, oxidative phosphorylation (OXPHOS) and fatty acid oxidation (FAO) were also markedly increased. Additionally, the expression level of TGF-β1, Smad2/3 and downstream pro-fibrotic markers was obviously upregulated. Taken together, we conclude that Cd activates the oxidative stress-mediated TLR4/NF-κB/NLRP3 inflammatory signal transduction, leading to porcine adrenal fibrosis by promoting macrophage polarization toward M1.

Chlorothalonil (CT) is a commonly used fungicide and its excessive application seriously threatens aquatic life and human health. Resveratrol (RSV) is a natural polyphenol and can be used as a therapeutic and preventive agent for the treatment of various diseases. To explore the toxic mechanism of CT exposure on fish kidney cell, as well as the alleviation effect of RSV, we established CT poisoning and/or RSV treatment fish kidney cell models. Ctenopharyngodon idellus kidney (CIK) cell line was treated with CT (5 μg/L) and/or RSV (10 μM) for 48 h. The results showed that CT exposure activated cytochromeP450s (CYPs) including CYP1A1, CYP1B1 and CYP1C, caused malondialdehyde (MDA) accumulation, inhibited glutathione (GSH) levels and glutathione peroxidase (GPX) activities, increased the expression of miR-15a and downregulated BCL2 and TNFα-induced protein 3 (TNFAIP3, A20), triggered mitochondrial pathway mediated apoptosis and receptor interacting serine/threonine kinase (RIP)-dependent necroptosis in CIK cells. However, cell death under CT exposure could be relieved by RSV treatment through inhibiting the expression of CYP1 family genes and restoring miR-15a/BCL2-A20 axis disorders. Overall, we conclude that RSV could relieve CT-induced apoptosis and necroptosis through miR-15a/Bcl2-A20 axis in CIK cells. These results enrich the toxicological mechanisms of the CT and confirm that RSV can be used as a potential antidote for CT poisoning.

Lead (Pb), as a toxic heavy metal pollutant, has been paid much attention. Pb is often discharged into the environment through the soot, wastewater and waste residue in industrial production, which poses a great threat to animal health. Selenium (Se) is a trace element known to antagonize the toxicity caused by heavy metals. However, the interaction between Se and Pb in chicken kidney and its specific biological mechanism are still unclear. So, we constructed chicken models of Pb exposure and Pb, Se co-exposure. Therefore, we used western blot and qRT-PCR to detect the expression of related genes. The results showed that Pb activated the MAPK signaling pathway by up-regulating the expression of MARK pathway genes to induce the expression of pro-apoptotic genes and necroptosis-related genes. Se can regulate the MARK signaling pathway and attenuated the expression of MAPK pathway genes altered by Pb to reduce apoptosis and necroptosis of chicken kidney cells. Our study gives new ideas for the specific mechanism of Pb nephrotoxicity and provides a reference for comparative medicine and clinical medication.

SiC is a newly developed photocatalyst, but it is often used together with a co-catalyst rather than solely for its relatively low activity. Here we reported the high activity of a commercial SiC in a photocatalysis and ozone combined process (photocatalytic ozonation). The commercial SiC showed very weak activity in photocatalysis of oxalic acid (OA) and para-hydroxybenzoic acid (PHBA) degradation, and its performance in catalytic ozonation was also not satisfied. However, the organics degradation and mineralization rates dramatically increased in the SiC photocatalytic ozonation. The different operation parameters were optimized, and the main reactive radicals in this process and its generation pathways were studied. It was found that photo-generated electron reduction of ozone and oxygen are the main pathways to produce hydroxyl radical, which was responsible to the high oxidizing ability of this process.

The evolution of oxygen-rich nanoparticles in selective laser melted 304L stainless steel (SLM 304L SS) and its effect on the mechanical property were studied by adjusting the heat treatment process. The nanoparticles mainly contained oxygen, chromium, and silicon; the average size of oxygen-rich nanoparticles increased with the temperature of heat treatment. The nanoparticles were Cr-rich phases when treated below 950 °C, while the nanoparticles were Si-rich oxides when treated at 1100 °C. With the increase in the temperature of heat treatment, the molten pool boundaries and cellular structures of SLM 304L SS gradually disappeared and the yield strength decreased obviously. The boundary of the cellular structure with high dislocation density provides a fast channel for atom diffusion, and thus it becomes the preferred nucleation site for nanoparticles. The dispersed nanoparticles are not only the strengthening phases but also can hinder the movement of dislocations, and thus they can greatly improve the strength of the material without sacrificing ductility.

Overuse and co-exposure of pesticides have become a public health problem and threat seriously water health and environmental organisms and even humans. Chlorothalonil (CT) and imidacloprid (IMI) are high-selling pesticides worldwide, which can persist in the environment, and present a series of severely toxic effects on non-target animals. However, the effect of co-application on aquatic organisms is unknown. Based on the concept of the toxic unit (TU), toxic interaction of CT and IMI was evaluated and showed the additive and synergistic toxicity on Ctenopharyngodon idellus (grass carp) kidney cell line (CIK cells). Cell death analysis found an obvious increase of the apoptosis and necrosis rates exposed to CT and IMI, and aggravation when applied together. Moreover, CT and IMI co-exposure accelerated the inhibition of CYP450s/ROS/HIF-1α signal, the decline of energy metabolism, mitochondrial dynamics disorder, activation of Bcl2/Bax/Cyt C/Casp3/Casp9 pathway and RIP1/RIP3/MLKL pathway. Bioinformatics analysis showed autophagy, cell response, NOD-like receptor signaling pathway might be affected by co-exposure. In summary, the above results indicate that co-exposure to CT and IMI has synergistic toxicity and aggravates cell death via inhibition of the CYP450s/ROS/HIF-1α signal. These data provide new insights for evaluating the stacking interaction and revealing the toxicological effects of pesticide mixture.

Dexmedetomidine (DEX) prevents kidney damage caused by sepsis, but the mechanism of this effect remains unclear. In this study, the protective molecular mechanism of DEX in lipopolysaccharide (LPS)-induced acute kidney injury was investigated and its potential pharmacological targets from the perspective of inhibiting oxidative stress damage and the nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasome activation. Intraperitoneal injection of DEX (30 μg/kg) significantly improved LPS (10 mg/kg) induced renal pathological damage and renal dysfunction. DEX also ameliorated oxidative stress damage by reducing the contents of reactive oxygen species, malondialdehyde and hydrogen peroxide, and increasing the level of glutathione, as well as the activity of superoxide dismutase and catalase. In addition, DEX prevented nuclear factor-kappa B (NF-κB) activation and I-kappa B (IκB) phosphorylation, as well as the expressions of NLRP3 inflammasome-associated protein and downstream IL-18 and IL-1β. The messengerRNA (mRNA) and protein expressions of toll-like receptor 4 (TLR4), NADPH oxidase-4 (NOX4), NF-κB, and NLRP3 were also significantly reduced by DEX. Their expressions were further evaluated by immunohistochemistry, yielding results were consistent with the results of mRNA and protein detection. Interestingly, the protective effects of DEX were reversed by atipamezole-an alpha 2 adrenal receptor (α2 AR) inhibitor, whereas idazoxan-an imidazoline receptor (IR) inhibitor failed to reverse this change. In conclusion, DEX attenuated LPS-induced AKI by inhibiting oxidative stress damage and NLRP3 inflammasome activation via regulating the TLR4/NOX4/NF-κB pathway, mainly acting on the α2 AR rather than IR.

In view of the single stereotype of memorizing knowledge points with the help of traditional books and easy to distract attention in the process of memorizing with the help of mobile phones and computers, a mobile handheld device, memory assistant, is designed and developed based on Ebbinghaus forgetting law curve, embedded software and hardware development technology, and memory list cycle pushing intelligent algorithm designed by forgetting curve law as the core. The experiment shows that the device is convenient and flexible like electronic devices such as mobile phones and computers, and also has the characteristics of high efficiency and concentration of book memorizing, at the same time, scientific and reasonable learning plan can effectively improve the efficiency of users’ Recitation and memory.

Nitrogen-containing heterocyclic contaminants (NHCs) have aroused intense environmental issues in waterbody because of their high toxicity and strong recalcitrance against natural degradation. In this study, ozonation degradation on four typical NHCs - benzotriazole (BTA), benzimidazole (BMZ), indazole (IDZ), and indole (IDO) was carried out to investigate the effects of N atoms on degradation efficiency. Global softness of the NHCs was calculated by density functional theory (DFT) and correlated with ozonation efficiency. It was discovered that higher global softness of the NHCs resulted in the greater degradation efficiency. Mineralization results together with the quenching tests results suggested that NHCs with ortho-positioned N atoms in heterocyclic ring are easier to be mineralized, while the presence of para-positioned N atoms increased the recalcitrance for destruction. Apart from hydroxyl radicals, superoxide radicals and singlet oxygens were also identified by electron paramagnetic resonance (EPR) spectra. Condensed Fukui index was employed to probe the potential active sites for both direct ozone oxidation and radical-based attack. Single point energies were further calculated among these active sites to seek the initial-stage hydroxylation intermediates. Combined the calculation results with the identified intermediates from time-of-flight mass spectroscopy (TOF-MS), degradation routes of the NHCs were proposed. This study discovered structure-dependent behavior of NHCs on ozonation efficiency and envisaged a more accurate strategy for establishing the degradation pathway.

Increasing evidence has demonstrated that dexmedetomidine (DEX) possesses multiple pharmacological actions. Herein, we explored the protective effect and potential molecular mechanism of DEX on lipopolysaccharide (LPS)-induced early acute kidney injury (AKI) from the perspective of antioxidant stress. We found that DEX (30 μg/kg, i.p.) ameliorated the renal dysfunction and histopathological damage (tubular necrosis, vacuolar degeneration, infiltration of inflammatory cells and cast formation) induced by LPS (10 mg/kg). DEX also attenuated renal oxidative stress remarkably in LPS-induced early AKI, as evidenced by reduction in production of reactive nitrogen species, decreasing malondialdehyde levels, as well as increasing superoxide dismutase activity and glutathione content. DEX prevented activator protein-1 translocation, inhibited phosphorylation of I-kappa B (IκB) and activation of nuclear factor kappa B (NF-κB) in LPS-induced early AKI, as assessed by real-time quantitative polymerase chain reaction and protein levels of c-Jun, c-Fos, IκB and NF-κB. Notably, DEX pretreatment had the same effect as intraperitoneal injection of an inhibitor of inducible nitric oxide synthase inhibitor (1400W; 15 mg/kg), and inhibited the activity of renal inducible nitric oxide synthase (iNOS) and decreased the expression of iNOS mRNA and NO production. However, the protective effect of DEX on LPS-induced early AKI was reversed by the alpha 2 adrenal receptor (α2-AR) inhibitor atipamezole, whereas the imidazoline receptor inhibitor idazoxan did not. Taken together, DEX protects against LPS-induced early AKI in rats by inhibiting the iNOS/NO signaling pathway, mainly by acting on α2-ARs instead of IRs.

This paper combines the domestic display software research and development work, the display process and main technologies are described, the model design and verification under kirin operating system was carried out, the standardization of data representation model and the generalized control display data interface are studied, and the data driven mechanism and control model has carried on the preliminary study and practive application, the rationality of the test of verify the feasibility of technology and framework.The application results show that the design of the new software is feasible and efficient, which reduces the difficulty of control development, improves the flexibility and scope of application of the control, greatly reduces the workload of software maintenance, and plays a good role in the follow-up research and development of the software.

The appearance of We-media marks that the audiences participate in cultural communication and interactions. But due to some users' attainment of We-media literacy is very low, causing many problems produced in the information communication, of which the media violence is extremely obvious. And its means of transmission makes the violence information' communication damage compared with traditional media for the spread is larger and more difficult to be controlled. Based on this reason, this paper will study the problem of media violence, and the regulatory method is put forward.

Web services are rapidly changing the landscape of software engineering. One of the most important technologies introduced by them is Web service composition (WSC). This paper proposes a rule-based service composition framework that supports user requirements and predefined business rules very well. The selection engine uses a multiobjective non-dominated sorting genetic algorithm (NSGA-II) which generates a set of Pareto optimal solutions that provides service consumers wider choices and thus helps increasing the decision support ability. Several experiments have been performed to simulate the selection algorithm and results show that it is effective, efficient and provides a new rule-based mechanism for service composition.

Web services are rapidly changing the landscape of software engineering. One of the most important technologies introduced by them is Web service composition (WSC). In this paper, we model the Quality of Service (QoS)-aware service composition problem as a multiobjective optimization problem (MOP). A corresponding algorithm that based on Nondominated Sorting Genetic Algorithm-II (NSGA-II) is proposed to solve the problem. The algorithm generates a set of Pereto optimal solutions which satisfy users' requirement. Our experimental results show that by using the proposed algorithm, we can give service consumers several different feasible solutions which make decision easier.