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Objectives To determine the prevalence of penicillin susceptibility among MSSA causing bloodstream infections (BSIs) in 16 Spanish hospitals and to characterize the penicillin-susceptible MSSA (MSSA-PENS) isolates. Methods A total of 1011 Staphylococcus aureus isolates were collected from blood cultures in 16 Spanish hospitals during 2018–19 (6–12 months) and their susceptibility to 18 antimicrobials was determined. The MSSA-PENS isolates were selected and examined by PCR to determine the presence of the blaZ gene, other resistance genes and the genes lukF/lukS-PV, eta, etb and tst. The immune evasion cluster (IEC) type was also analysed. All the MSSA-PENS isolates were submitted to S. aureus protein A (spa) typing and the clonal complexes (CCs) were assigned according to their spa type. Results The prevalence of MSSA was 74.6% (754/1011) and 14.9% (151/1011) were MSSA-PENS-blaZnegative. MSSA-PENS-blaZnegative isolates (n = 151) were ascribed to 88 spa types and 11 CCs. The most frequent CCs were CC5 (35/151) and CC398 (25/151), with t002-CC5 and t571-CC398 being the most common lineages. Pan-susceptibility was identified in 117 of the 151 MSSA-PENS-blaZnegative isolates (77.5%). In the remaining isolates, erythromycin and clindamycin resistance was the most frequent resistance found, although tobramycin, ciprofloxacin, fusidic acid, mupirocin and/or tetracycline resistance was also detected. Thirty-eight MSSA-PENS-blaZnegative isolates were IEC negative and four isolates were Panton–Valentine leucocidin (‘PVL’) positive. Conclusions A high penicillin susceptibility rate was detected among MSSA, opening therapeutic opportunities for BSIs. The emergence of new successful MSSA-PENS clones could be responsible for these data. The detection among MSSA-PENS-blaZnegative isolates of the clonal lineage CC398 or the absence of an IEC raises questions about their possible animal origin, requiring further analysis.

2 pages.-- Nomenclatural notes., Article was realized with a research grant by the Ministerio de Educación y Ciencia of Spain (Research project: Fauna Ibérica VII; Research sub-project: Fauna íbero-balear de Aphidinae (Hemiptera: Aphididae): tribu Aphidini y primera parte de la tribu Macrosiphini, code number: REN2001-1956-C17-03/GLO.

Reasons for performing study: Staphylococcus intermedius group (SIG) bacteria can colonise the nares of some animals but are also emerging pathogens in humans and animals. Objectives: To analyse SIG nasal carriage in healthy donkeys destined for food consumption in Tunisia and to characterise recovered isolates. Methods: Nasal swabs from 100 healthy donkeys were tested for SIG recovery, and isolates were identified by biochemical and molecular methods. Antimicrobial susceptibility of isolates was tested and detection of antimicrobial resistance and virulence genes was performed. Isolates were typed at the clonal level by multilocus sequence typing and SmaI pulsed-field gel electrophoresis. Results: Staphylococcus delphini and Staphylococcus pseudintermedius (included in SIG) were obtained in 19% and 2% of the tested samples, respectively, and one isolate per sample was characterised. All isolates were meticillin susceptible and mecA negative. Most S.delphini and S.pseudintermedius isolates showed susceptibility to all antimicrobials tested, with the exception of 2 isolates resistant to tetracycline (tet(M) gene) or fusidic acid. The following toxin genes were identified (percentage of isolates): lukS-I (100%), lukF-I (9.5%), siet (100%), se-int (90%), seccanine (19%) and expA (9.5%). Thirteen different pulsed-field gel electrophoresis profiles were identified among the 21 SIG isolates. Additionally, the following 9 different sequence types (STs) were detected by multilocus sequence typing, 6 of them new: ST219 (6 isolates), ST12 (5 isolates), ST220 (3 isolates), ST13, ST50, ST193, ST196, ST218 and ST221 (one isolate each). Conclusions: Staphylococcus delphini and S.pseudintermedius are common nasal colonisers of donkeys, generally susceptible to the antimicrobials tested; nevertheless, these SIG isolates contain virulence genes, including the recently described exfoliative gene (expA) and several enterotoxin genes, with potential implications for public health. This is the first description of S. delphini in Tunisia. The Summary is available in Chinese - see Supporting information. © 2014 EVJ Ltd.

A review of extinct and living amphibians known from fossils (Allocaudata, Anura and Caudata) has revealed several cases that require nomenclatural changes in order to stabilize the taxonomy of the group. Nomenclatural changes include homonym replacements, corrections of spelling variants and authorships, name availabilities, and in particular, the proposal of new combinations. These changes will allow the incorporation of some palaeontological taxa to the current evolutionary models of relationship of modern forms based on molecular phylogenies. Rana cadurcorum for Rana plicata Filhol, 1877, Rana auscitana for Rana pygmaea Lartet, 1851, and Rana sendoa for Rana robusta Brunner, 1956. Anchylorana Taylor, 1942 is considered a new synonym of Lithobates Fitzinger, 1843. New combinations proposed are: Anaxyrus defensor for Bufo defensor Meylan, 2005; Anaxyrus hibbardi for Bufo hibbardi Taylor, 1937; Anaxyrus pliocompactilis for Bufo pliocompactilis Wilson, 1968; Anaxyrus repentinus for Bufo repentinus Tihen, 1962; Anaxyrus rexroadensis for Bufo rexroadensis Tihen, 1962; Anaxyrus spongifrons for Bufo spongifrons Tihen, 1962; Anaxyrus suspectus for Bufo suspectus Tihen, 1962; Anaxyrus tiheni for Bufo tiheni Auffenberg, 1957; Anaxyrus valentinensis for Bufo valentinensis Estes et Tihen, 1964; Ichthyosaura wintershofi for Triturus wintershofi Lunau, 1950; Incilius praevius for Bufo praevius Tihen, 1951; Lithobates bucella for Rana bucella Holman, 1965; Lithobates dubitus for Anchylorana dubita Taylor, 1942; Lithobates fayeae for Rana fayeae Taylor, 1942; Lithobates miocenicus for Rana miocenica Holman, 1965; Lithobates moorei for Anchylorana moorei Taylor, 1942; Lithobates parvissimus for Rana parvissima Taylor, 1942; Lithobates rexroadensis for Rana rexroadensis Taylor, 1942; Lithobates robustocondylus for Anchylorana robustocondyla Taylor, 1942; Ommatotriton roehrsi for Triturus roehrsi Herre, 1955; Pelophylax barani for Rana barani Rückert-Ülkumen, 1980; Pelophylax meriani for Rana meriani Meyer, 1853; Pelophylax pueyoi for Rana pueyoi Navás, 1922a; Pelophylax quellenbergi for Rana quellenbergi Navás, 1922; Philoria borealis for Kyarranus borealis Tyler, 1991; Pseudepidalea belogorica for Bufo belogoricus Ratnikov, 1993; Pseudepidalea plana for Bufo planus Ratnikov, 1993; Pseudepidalea prisca for Bufo priscus Spinar, Klembara et Meszáros, 1993, and Pseudepidalea stranensis for Bufo stranensis Nemec, 1972. The names Geyeriellinae Brame, 1958, Palaeurodelidae Brame, 1958, Prosalamandridae Stefano, 1903, Lipelucidae Huene, 1956, Rana temporaria fossilis Stefanov, 1951, Salteniidae Kuhn, 1962, Vieraellidae Reig, 1961, and Voigtiellinae Brame, 1958 are nomenclaturally deemed unavailable. The family name based on Scapherpeton Cope, 1876 is Scapherpetidae and not Scapherpetonidae nor Scapherpetontidae.Una revisión de anfibios extintos y actuales en estado fósil (Allocaudata, Anura y Caudata) ha permitido detectar diversos casos que precisan cambios nomenclaturales a fin de estabilizar la taxonomía del grupo. Los cambios nomenclaturales incluyen homonimias, correcciones de variantes gramaticales y autorías, disponibilidad de nombres, y en especial la propuesta de nuevas combinaciones, necesarias para ajustar algunos taxones paleontológicos a los modelos de relaciones evolutivas entre formas vivientes, fundamentados en filogenias moleculares. Las nuevas combinaciones que se proponen son: Anaxyrus defensor para Bufo defensor Meylan, 2005; Anaxyrus hibbardi para Bufo hibbardi Taylor, 1937; Anaxyrus pliocompactilis para Bufo pliocompactilis Wilson, 1968; Anaxyrus repentinus para Bufo repentinus Tihen, 1962; Anaxyrus rexroadensis para Bufo rexroadensis Tihen, 1962; Anaxyrus spongifrons para Bufo spongifrons Tihen, 1962; Anaxyrus suspectus para Bufo suspectus Tihen, 1962; Anaxyrus tiheni para Bufo tiheni Auffenberg, 1957; Anaxyrus valentinensis para Bufo valentinensis Estes et Tihen, 1964; Ichthyosaura wintershofi para Triturus wintershofi Lunau, 1950; Incilius praevius para Bufo praevius Tihen, 1951; Lithobates bucella para Rana bucella Holman, 1965; Lithobates dubitus para Anchylorana dubita Taylor, 1942; Lithobates fayeae para Rana fayeae Taylor, 1942; Lithobates miocenicus para Rana miocenica Holman, 1965; Lithobates moorei para Anchylorana moorei Taylor, 1942; Lithobates parvissimus para Rana parvissima Taylor, 1942; Lithobates rexroadensis para Rana rexroadensis Taylor, 1942; Lithobates robustocondylus para Anchylorana robustocondyla Taylor, 1942; Ommatotriton roehrsi para Triturus roehrsi Herre, 1955; Pelophylax barani para Rana barani Rückert-Ülkumen, 1980; Pelophylax meriani para Rana meriani Meyer, 1853; Pelophylax pueyoi para Rana pueyoi Navás, 1922a; Pelophylax quellenbergi para Rana quellenbergi Navás, 1922; Philoria borealis para Kyarranus borealis Tyler, 1991; Pseudepidalea belogorica para Bufo belogoricus Ratnikov, 1993; Pseudepidalea plana para Bufo planus Ratnikov, 1993; Pseudepidalea prisca para Bufo priscus Spinar, Klembara et Meszáros, 1993, y Pseudepidalea stranensis para Bufo stranensis Nemec, 1972. Los nombres Geyeriellinae Brame, 1958, Palaeurodelidae Brame, 1958, Prosalamandridae Stefano, 1903, Lipelucidae Huene, 1956, Rana temporaria fossilis Stefanov, 1951, Salteniidae Kuhn, 1962, Vieraellidae Reig, 1961, y Voigtiellinae Brame, 1958 se consideran nomenclaturalmente no disponibles. El nombre de familia basado en Scapherpeton Cope, 1876 es Scapherpetidae y no Scapherpetonidae ni Scapherpetontidae.

We present an up to date synonymic list of the Iberian and Balearic fauna of Meloidae (Coleoptera). This current list includes all synonymies for 65 taxa at the species level. We have checked most of the original references for all taxa and names cited in the catalogue. This revision leads to various corrections and changes in the spelling of some names and in publication dates and pagination of original references with respect to previous catalogues. The work is illustrated with photographs of 13 type specimens. Some nomenclatural and taxonomic problems require additional work, and their solution might produce further changes in the current list. Most relevant among these problems are the revision of M. maculosopunctata nomenclature, the confirmation of the specific identification of the single Iberian record for M. tricincta, the revision of the taxonomic status of Meloe ibericus, herein maintained at the species level while waiting for the needed revision of the M. brevicollis species group, the evaluation of the taxonomic status of Sitaris melanura and the taxonomic problem of the subgenus Meloe in the Balearic Islands. En este trabajo presentamos un listado sinonímico actualizado de la fauna de Meloidae (Coleoptera) de la Península Ibérica e Islas Baleares. En el listado actual se recogen las sinonimias correspondientes a 65 táxones de nivel especie. Se han revisado las publicaciones donde se incluyen las descripciones originales de la mayoría de los táxones y nombres de las especies ibero baleares de Meloidae. Esta revisión ha llevado a diversas correcciones y actualizaciones tanto en la grafía de algunos nombres, como en las las referencias originales, incluyendo cambios de fechas o de paginación con respecto a catálogos anteriores. El trabajo se ilustra con fotografías de 13 ejemplares tipo. Existen una serie de problemas nomenclaturales y taxonómicos aún por resolver, que podrían producir cambios en el listado actual. Entre estos problemas destacan el caso de la nomenclatura de M. maculosopunctata que requiere revisión, la adscripción específica correcta de la cita ibérica de M. tricincta, la revisión del estatus taxonómico de Meloe ibericus, que se mantiene a nivel específico en espera de la revisión del grupo de especies afines a M. brevicollis, la revisión del estatus de Sitaris melanura y el problema taxonómico del subgénero Meloe en las Islas Baleares.

The study of 131 more or less complete Curculionoid specimens of the collection Étienne Louis Geoffroy, conserved in the Muséum d’Histoire Naturelle de Paris (Entomologie) has permitted the identification of several nominal species that were nomina dubia and the establishment of several new synonymies and combinations, and, in some cases, the reversion of precedence following Art. 23.9 of the Code, declaring nomina protecta and nomina oblita. New synonymies are (the first term is the valid name): Lixus filiformis (Fabricius, 1781) = Curculio longus Gmelin, 1790; Lasiorhynchites cavifrons (Gyllenhal, 1833) nom. protectum = Rhinomacer viridis Geoffroy, 1785, nom. oblitum; Byctiscus betulae (Linnaeus, 1758) = Rhinomacer auratus Geoffroy, 1785; Neocoenorrhinus pauxillus (Germar, 1824) nom. protectum = Rhinomacer caeruleus Geoffroy, 1785, nom. oblitum; Deporaus betulae (Linnaeus, 1758) = Curculio nigrostriatus Goeze, 1777 = Rhinomacer niger Geoffroy, 1785 = Curculio fuliginosus Gmelin, 1790; Coniocleonus hollbergii (F√•hraeus, 1842) = Curculio sulcatus Goeze, 1777 = Curculio sulcatus Geoffroy, 1785 = Curculio sulcatus Gmelin, 1790; Larinus iaceae (Fabricius, 1775) = Curculio carduelis Goeze, 1777; Hypera postica (Gyllenhal, 1813), nom. protectum = Curculio fasciolatus Geoffroy, 1785, nom. oblitum; Charagmus griseus (Fabricius, 1775) = Curculio cupreosquamosus Goeze, 1777 = Curculio intersectus Geoffroy, 1785 = Curculio squamosus Gmelin, 1790; Sitona hispidulus (Fabricius, 1777) = Curculio griseus Goeze, 1777 = Curculio modestus Geoffroy, 1785 = Curculio geoffroaei Gmelin, 1790; Aulacobaris cuprirostris (Fabricius, 1787) = Curculio viridisericeus Goeze, 1777; Cleopomiarus plantarum (Germar, 1824), nom. protectum = Curculio nigrostriatus Goeze, 1777 = Curculio floriger Geoffroy, 1785, nom. oblitum = Curculio subglobosus Gmelin, 1790, nom. oblitum; Anisorhynchus scabrosus (Geoffroy, 1785) = Curculio barbatus Rossi, 1794; Hylobius abietis (Linnaeus, 1758) = Curculio tigris Gmelin, 1790; Sitophilus granarius (Linnaeus, 1758) = Curculio contractus Geoffroy, 1785; Phyllobius pomaceus Gyllenhal, 1834 = Curculio auratus Geoffroy, 1785; Hylobius transversovittatus (Goeze, 1777) = Curculio fascialis Gmelin, 1790. New combinations are: Leucophyes occidentalis (Dieckmann, 1982) (from Leucosomus) and Anisorhynchus scabrosus (Geoffroy, 1785) (from Curculio). The following names have been reinstated (stat. res.) as valid: Trachyphloeus spinosus (Goeze, 1777) for the species known as Trachyphloeus olivieri Bedel, 1883, Lixus pulverulentus (Scopoli, 1763) for the species known as Lixus angustatus (Fabricius, 1775). Hypera melancholica (Fabricius, 1793) is confirmed as the valid name for the species hitherto named Hypera fuscocinerea (Marsham, 1802) or Hypera murina (Fabricius, 1793). The following names are considered nomina dubia, since there is no identifiable type material: Curculio pulex Goeze, 1777, Rhinomacer minutus Geoffroy, 1785, Curculio pulex Gmelin, 1790 (non Goeze, 1777), Rhinomacer striatusi> Geoffroy, 1785, Rhinomacer fulgidusi> Geoffroy, 1785, Curculio transversofasciatus Goeze, 1777, Curculio fasciatus Geoffroy, 1785 (non Scopoli, 1763, nec Str√∂m, 1768, nec Degeer, 1775, nec Mu.ller, 1776), Curculio fuscatus Gmelin, 1790, Curculio sulcatus Goeze, 1777: 410 (non Fabricius, 1775, nec Goeze, 1777: 381), Curculio incisus Geoffroy, 1785, Curculio exaratus Gmelin, 1790, Curculio quadratus Goeze, 1777, Curculio quadrilis Geoffroy, 1785, Curculio griseosericeus Goeze, 1777, Curculio communis Geoffroy, 1785, Curculio thoracespinosus Goeze, 1777, Curculio armatus Geoffroy, 1785, Curculio denticulatus Gmelin, 1790 (non Schrank, 1781, nec Geoffroy, 1785), Curculio muricatus Goeze, 1777 (non Drury, 1773), Curculio murinus Geoffroy, 1785 (non Müller, 1764), Curculio fuscipes Geoffroy, 1785, Curculio tesselatus Geoffroy, 1785 (non Fabricius, 1781), Curculio sericeus Goeze, 1777, Curculio villosus Geoffroy, 1785 (non Fabricius, 1781), Curculio sericeus Gmelin, 1790 (non Goeze, 1777, nec Schaller, 1783, nec Piller & Mitterpacher, 1783), Curculio virgo Goeze, 1777, Curculio virgo Geoffroy, 1785 (non Goeze, 1777), Curculio virgo Gmelin, 1790 (non Goeze, 1777, nec Geoffroy, 1785), Curculio punctulatus Geoffroy, 1785.El estudio de 131 ejemplares de Curculiónidos, más o menos completos, de la colección Étienne Louis Geoffroy conservados en el Muséum d’Histoire Naturelle de París (Entomología) ha permitido identificar varias especies nominales que eran consideradas nomina dubia y establecer varias nuevas sinonimias y combinaciones, e incluso, en algunos casos, recuperar la prioridad de acuerdo con el Art. 23.9 del Código, declarando nomina protecta y nomina oblita. Las nuevas sinonimias son (se indica en primer lugar el nombre válido): Lixus filiformis (Fabricius, 1781) = Curculio longus Gmelin, 1790; Lasiorhynchites cavifrons (Gyllenhal, 1833) nom. protectum = Rhinomacer viridis Geoffroy, 1785, nom. oblitum; Byctiscus betulae (Linnaeus, 1758) = Rhinomacer auratus Geoffroy, 1785; Neocoenorrhinus pauxillus (Germar, 1824) nom. protectum = Rhinomacer caeruleus Geoffroy, 1785, nom. oblitum; Deporaus betulae (Linnaeus, 1758) = Curculio nigrostriatus Goeze, 1777 = Rhinomacer niger Geoffroy, 1785 = Curculio fuliginosus Gmelin, 1790; Coniocleonus hollbergii (Fåhraeus, 1842) = Curculio sulcatus Goeze, 1777 = Curculio sulcatus Geoffroy, 1785 = Curculio sulcatus Gmelin, 1790; Larinus iaceae (Fabricius, 1775) = Curculio carduelis Goeze, 1777; Hypera postica (Gyllenhal, 1813), nom. protectum = Curculio fasciolatus Geoffroy, 1785, nom. oblitum; Charagmus griseus (Fabricius, 1775) = Curculio cupreosquamosus Goeze, 1777 = Curculio intersectus Geoffroy, 1785 = Curculio squamosus Gmelin, 1790; Sitona hispidulus (Fabricius, 1777) = Curculio griseus Goeze, 1777 = Curculio modestus Geoffroy, 1785 = Curculio geoffroaei Gmelin, 1790; Aulacobaris cuprirostris (Fabricius, 1787) = Curculio viridisericeus Goeze, 1777; Cleopomiarus plantarum (Germar, 1824), nom. protectum = Curculio nigrostriatus Goeze, 1777 = Curculio floriger Geoffroy, 1785, nom. oblitum = Curculio subglobosus Gmelin, 1790, nom. oblitum; Anisorhynchus scabrosus (Geoffroy, 1785) = Curculio barbatus Rossi, 1794; Hylobius abietis (Linnaeus, 1758) = Curculio tigris Gmelin, 1790; Sitophilus granarius (Linnaeus, 1758) = Curculio contractus Geoffroy, 1785; Phyllobius pomaceus Gyllenhal, 1834 = Curculio auratus Geoffroy, 1785; Hylobius transversovittatus (Goeze, 1777) = Curculio fascialis Gmelin, 1790. Las nuevas combinaciones son: Leucophyes occidentalis (Dieckmann, 1982) (desde Leucosomus) y Anisorhynchus scabrosus (Geoffroy, 1785) (desde Curculio). Se han restablecido como válidos los siguientes nombres (stat. res.): Trachyphloeus spinosus (Goeze, 1777) para la especie conocida como Trachyphloeus olivieri Bedel, 1883, y Lixus pulverulentus (Scopoli, 1763) para Lixus angustatus (Fabricius, 1775). Se confirma como nombre válido Hypera melancholica (Fabricius, 1793) para la especie denominada hasta ahora Hypera fuscocinerea (Marsham, 1802) o Hypera murina (Fabricius, 1793). Al no poder indentificar el material tipo se consideran nomina dubia los siguientes nombres: Curculio pulex Goeze, 1777, Rhinomacer minutus Geoffroy, 1785, Curculio pulex Gmelin, 1790 (non Goeze, 1777), Rhinomacer striatus Geoffroy, 1785, Rhinomacer fulgidus Geoffroy, 1785, Curculio transversofasciatus Goeze, 1777, Curculio fasciatus Geoffroy, 1785 (non Scopoli, 1763, nec Ström, 1768, nec Degeer, 1775, nec Müller, 1776), Curculio fuscatus Gmelin, 1790, Curculio sulcatus Goeze, 1777: 410 (non Fabricius, 1775, nec Goeze, 1777: 381), Curculio incisus Geoffroy, 1785, Curculio exaratus Gmelin, 1790, Curculio quadratus Goeze, 1777, Curculio quadrilis Geoffroy, 1785, Curculio griseosericeus Goeze, 1777, Curculio communis Geoffroy, 1785, Curculio thoracespinosus Goeze, 1777, Curculio armatus Geoffroy, 1785, Curculio denticulatus Gmelin, 1790 (non Schrank, 1781, nec Geoffroy, 1785), Curculio muricatus Goeze, 1777 (non Drury, 1773), Curculio murinus Geoffroy, 1785 (non Müller, 1764), Curculio fuscipes Geoffroy, 1785, Curculio tesselatus Geoffroy, 1785 (non Fabricius, 1781), Curculio sericeus Goeze, 1777, Curculio villosus Geoffroy, 1785 (non Fabricius, 1781), Curculio sericeus Gmelin, 1790 (non Goeze, 1777, nec Schaller, 1783, nec Piller & Mitterpacher, 1783), Curculio virgo Goeze, 1777, Curculio virgo Geoffroy, 1785 (non Goeze, 1777), Curculio virgo Gmelin, 1790 (non Goeze, 1777, nec Geoffroy, 1785), Curculio punctulatus Geoffroy, 1785.

Publishing taxonomic and systematics studies in the digital era faces major challenges and requires new approaches, many of which are currently stimulating spirited discussions amongst taxonomists and systematists. New amendments to the International Code of Zoological Nomenclature are expected to regulate electronic publishing of new taxa and create a standard form for their registration (ZooBank). Responding to a perceived need, this editorial announces establishment of ZooKeys - a new online and print journal in zoological taxonomy and systematics, which aims to quickly respond and adapt to the newest developments in taxonomic publishing. Open Access is accepted as mandatory for ZooKeys. Th e rationale for and concept of ZooKeys is discussed in detail.

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A checklist of the Coleoptera Curculionoidea of Comunidad de Madrid (Spain) is presented for the first time. It includes 522 species in 175 genera belonging to 10 families. Se presenta por primera vez un listado de los Coleoptera Curculionoidea de la Comunidad de Madrid (España), que incluye 552 especies distribuidas en 175 géneros pertenecientes a 10 familias.

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This paper is the first result of an agreement between the Organismo Autónomo Parques Nacionales and the Consejo Superior de Investigaciones Científicas, entitled “Inventory and study of the Invertebrate Fauna of the National Park of La Caldera de Taburiente”. A detailed account of the faunistic novelties found up to now among the specimens of terrestrial and freshwater invertebrates collected along the two years of sampling (July 1999 to July 2001), whose number is estimated in ca. 500,000, is given. A brief description of planning and of methodology applied to the inventory of the invertebrate fauna is made and a tabulated summary of novelties is presented. The list shows at present 284 families, 594 genera and 739 species. For the Canary Islands, the present record of new taxa is 29 families, 115 genera (1 of them confirmed as new to Science and 3 awaiting confirmation) and 187 species (24 new to Science). Moreover, 242 genera and 338 species are new to the fauna of La Palma I., being known from other islands of the archipelago. Other 47 taxa, still being studied, could be new to Science as well. These results have been reached with the study of just a minimal part of the whole material, which underlines the need for systematic, continued sampling to evaluate the faunistic richness of poorly explored areas and its possible necessity for protection. Therefore, one should expect more novelties and the inventory may increase significantly when all the material is revised. El presente artículo es el primer resultado de un convenio entre el Organismo Autónomo Parques Nacionales y el Consejo Superior de Investigaciones Científicas, denominado “Inventario y estudio de la Fauna Invertebrada del Parque Nacional de La Caldera de Taburiente”. Se detallan las novedades faunísticas encontradas hasta el momento entre los ejemplares de invertebrados terrestres y dulceacuícolas recogidos durante los dos años de muestreo (julio de 1999 a julio de 2001), cuyo número se estima en unos 500.000. Se hace una breve descripción de la planificación y la metodología aplicadas al inventario de la fauna invertebrada y se tabulan las novedades. En su estado actual el inventario arroja 284 familias, 594 géneros y 739 especies. Para la fauna de las Islas Canarias, el registro actual de táxones nuevos es de 29 familias, 115 géneros (de los cuales 1 género ha sido confirmado como nuevo para la Ciencia y otros 3 están pendientes de confirmación) y 187 especies (de las cuales 24 nuevas para la Ciencia). Además, 242 géneros y 338 especies son nuevas para la fauna de la isla de La Palma, conociéndose con anterioridad de otras islas del archipiélago. Otros 47 táxones aún en estudio, podrían resultar asimismo nuevos para la Ciencia. Estos resultados se han alcanzado tan sólo con el estudio de una parte mínima del material, lo que subraya la necesidad de muestreos continuados y sistemáticos para evaluar la riqueza faunística de áreas poco exploradas y su posible necesidad de protección. Por tanto, cabe esperar más novedades y el inventario puede quedar significativamente incrementado cuando se revise todo el material.

Forty-two lactic acid bacteria (LAB) of the genera Lactobacillus (32), Leuconostoc (6), Pediococcus (3) and Lactococcus (1), isolated from Rioja red Wines, were tested for antimicrobial activity. All these strains, as well as 18 Leuconostoc oenos and 19 yeast strains were used as indicators. Only nine strains showed antimicrobial activity, and all were of the species Lactobacillus plantarum, which constitutes the predominant micro flora in Rioja red wines after alcoholic fermentation. Lact. plantarum strain J-51 showed the widest range of action, inhibiting the growth of 31 strains of the four studied LAB genera. Lact. plantarum J-51 antimicrobial activity was lost after treatment with proteases, suggesting a proteinaceous nature for this activity. It was found to be stable between pH 3 and 9 and under strong heating conditions (100 °C for 60 min). Polymerase chain reaction (PCR) of Lact. plantarum J-51 genome the presence analysis revealed of the plnA gene that encodes the plantaricin precursor PlnA. A 366-bp fragment was sequenced and showed 95% identity with pln locus of Lact. plantarum C-11. The deduced precursor peptide sequence showed one mutation (Gly7 to Ser7) at the double glycine leader peptide, and the three putative 26-, 23- and 22-residue active peptides remain identical to those of Lact. plantarum C-11. Therefore, antimicrobial peptides constitute a potent adaptation advantage for those strains that dominate in a medium such as wine, and can play an important role in the ecology of wine microflora.

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Melolontha emarginata Gyllenhal, 1817 is designated as the type species of Elaphocerida Reitter, 1902. The type specimens of the species of genus Elaphocera Gené, 1836, from the entomological collections of Museo Nacional de Ciencias Naturales (Madrid, Spain), are studied. Lectotypes are designated for species Elaphocera (Elaphocerida) ampla Báguena, 1955, Elaphocerida segurensis Escalera, 1923, Elaphocerida pygmaea Escalera, 1923 and Elaphocera (Elaphocerida) torressalai Báguena, 1955. The type material of the following species is also revised: Elaphocera pardoi Escalera, 1931, Elaphocera (Elaphocerida) ibicensis Escalera, 1926, Elaphocerida abdelkrimi Escalera, 1934 and Elaphocera baguenae Mancini, 1926. En este trabajo se designa Melolontha emarginata Gyllenhal, 1817 como la especie tipo de Elaphocerida Reitter, 1902. Se revisan las series típicas de las especies del género Elaphocera Gené, 1836 conservadas en las colecciones de Entomología del Museo Nacional de Ciencias Naturales (Madrid, España). Se designan los lectotipos de Elaphocera (Elaphocerida) ampla Báguena, 1955, Elaphocerida segurensis Escalera, 1923, Elaphocerida pygmaea Escalera, 1923 y Elaphocera (Elaphocerida) torressalai Báguena, 1955. Además, se proporcionan nuevos datos sobre el material típico de las especies Elaphocera pardoi Escalera, 1931, Elaphocera (Elaphocerida) ibicensis Escalera, 1926, Elaphocerida abdelkrimi Escalera, 1934 y Elaphocera baguenae Mancini, 1926, conservado en estas colecciones.

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The protein fraction of white musts and wines obtained from grapes (Vitis vinifera L. cv. Viura) grown in northern Spain (Rioja) was investigated by SDS-PAGE. Under different fermentation conditions, e.g. aeration, must nitrogen content and yeast strain, SDS-PAGE patterns showed several bands with molecular weights ranging from 16 to 200 kDa. Higher weight proteins were glycosylated, whereas lower weight proteins were not. Under the experimental fermentation conditions none of the proteins showed any chemical modification that would alter the electrophoretical mobility or the covalent binding to their glycosylated moiety., VITIS - Journal of Grapevine Research, Vol. 37 No. 3 (1998): Vitis