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Additional file 1: Fig. S1. Information of individual human amnion sample. Fig. S2. Expression of established markers in six cell types of the human amnion. Fig. S3. Feature plots of immune cell marker expression in immunocytes of the human amnion. Fig. S4. GO analysis of highly-expressed genes in subclusters 1 and 4 of immunocytes of the human amnion. Fig. S5. Top DEGs in individual subclusters of EpC_FB of the human amnion. Fig. S6. Immunohistochemical staining of CCL20 in the human amnion in TNL_P, TL_D and TL_P groups. Fig. S7. Negative control of immunohistochemical staining of CD45 in mouse intrauterine tissues. Table S1. Information of each individual sample. Table S2. Cell number and proportion of each cell type. Table S3. Demographic and clinical characteristics of recruited pregnant women.

The determination of gas well productivity is the basis of scientific and reasonable development of gas fields and has important guiding significance for gas well production allocation. According to the change of gas-water two-phase seepage law, the productivity equation of horizontal well with gas-water production in low permeability gas reservoir was established based on the theory of steady seepage, which was affected by stress sensitivity, formation damage and high-speed non-Darcy. The results show that the effective thickness, horizontal section length, permeability and well radius are positively correlated with productivity, while the water saturation, stress sensitivity, well control radius, skin factor and formation temperature are negatively correlated, and the water saturation and stress sensitivity have a greater impact on productivity.