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Additional file 1: Table S1. Plasmids and E. coli stains used in this study. Table S2. Primers used in this study. Table S3. The schematic and sequences of the editing cassette used in this study. Table S4. The sequences of clone PCR products used to identity the E. coli variants. Figure S1. Stability of the chromosomal organization of E. coli0.10/4.54 and E. coli2.28/2.36. (A) Colony PCR analysis of the E. coli0.10/4.54 cells after culturing for more than 100 generations using the primer pairs f1/r1, f2/r2, f1/r2 and f2/r1. (B) Colony PCR analysis of E. coli2.28/2.36 after culturing for more than 100 generations using the primer pairs f3/r3, f4/r4, f3/r4 and f4/r3. Figure S2. Stability of the chromosomal organization of E. coli2.28/2.36 (mix with E. coliMut). A total of 11 rounds of inoculation were conducted, corresponding to approximately 100 generations. The culture of the first (0 generation), third (about 30 generations), fifth (about 50 generations), seventh (about 70 generations), ninth (about 90 generations) and last (about 100 generations) round of E. coli2.28/2.36 (mix with E. coliMut) was spread on LB agar plates, 24 single colonies of each strain were selected and four pairs of primers f5/r5, f6/r6, f5/f6 and r5/r6 were used to investigate the speed of transition of genomic instability with the passage of generations.

Additional file 1: Figure S1. The confocal microscopy revealed that HMC3 cells phagocytized exosomes released by U251 and U87 cells. Figure S2. Radiotherapy increased the number of exosomes secreted by glioblastoma multiforme (GBM) cells. A Transmission electron microscopy demonstrated that the secreted exosomes were rounded particles with a double-layer membrane. B The size of these exosomes was approximately 80–100 nm. C Nanoparticle tracking analysis indicated that radiotherapy increased the number of exosomes secreted by U251 and U87 cells. Table S1 All oligonucleotide sequences and Table S2. All primers of RT-PCR.

Fe-based amorphous coatings were synthesized with Fe44Cr30Mo20B3C3 powders at different laser powers on Q235 substrate at different laser powers. The microstructure and mechanical property of the coatings were investigated. X-ray diffraction and the thermodynamic analysis results indicate the presence of both amorphous and crystalline phases in the coating. The highest hardness up to 917 HV0.5 is obtained at 1300 W, which is about 5 times that of the substrate. From the abrasive test, the coating obtained at lower laser power exhibits shallower grooves and lower weight loss, indicating better wear resistance. The synergism of the amorphous phase and Cr23C6 contributes to the improvement of the mechanical properties. The existence of Cr and the amorphous phase also promotes the passivation phenomenon and improves the corrosion resistance of the coating.

With the advent of information age in 21th century, it is becoming an inevitable trend of development and survival for coal enterprises to use information means to ensure safety production. However, coal enterprises possess a lot of special characteristics, such as complex geographical environment and large amount of information. Certain functions including traditional underground monitoring system and dispatching telephones act on their own ways which leads to a bad coordination. This paper takes full consideration of personnel transferring, material transferring and information transferring and use .net technology to construct coal mine safety monitoring system. We use human-machine-integration method to evaluate working safety and provide well-developed security for production safety of coal enterprises.

Internet of things is becoming more and more important in both industrial and consumer sides according to development of network technologies, especially the high development of mobile internet. The resources size is also becoming larger and larger. Since all resources are allocated by addressing system, it would cost a long time in current solutions with a higher repletion frequency. Therefore, this paper researched on Zigbee based resources address system and designed its addressing procedures, network establishment strategy and data process system. After stimulate with conventional distributed addressing system and centralized system, the result shows this paper designed Zigbee based resources addressing system can work well with a lower process time and repletion frequency.

Zhang Chunzhi1,2, Jiang Tao3, Wang Jinhuan4, Cheng Jinquan5, Pu Peiyu2 and Kang Chunsheng2 1Department of Radiation Oncology, Tianjin Huanhu hospital, Tianjin, 2Department of Neurosurgery, Laboratory of Neuro-Oncology, Tianjin Medical University General Hospital, Tianjin Key Laboratory of Nerve Injury, Variation and Regeneration, Tianjin, 3Department of Neurosurgery, Tiantan Hospital, Capital Medical University, Beijing, 4Department of Neurosurgery, Tianjin Huanhu hospital, Tianjin 5Department of Molecular Oncology, H.Lee Moffitt Cancer and Research Institute, Tampa, 1,2,3,4China 5USA

A dynamic model has been found in this article based on multi-body system dynamic theory, which can be used of researching of the dynamic characteristic of scraper conveyor sprocket transmission system. The dynamic model includes sprockets, chains, middle troughs, transitional troughs and scrapers. The main parameters of components are considered, such as mass parameter, inertia parameter, geometers parameter and constructional parameters, etc. According to interaction characteristic between main components, high contact pairs are mainly used to modeling the dynamic model. As an example, dynamic simulation is carried out on the condition of electromotor rated power output. And then, dynamic loads of sprocket and chains are exported and analyzed. The dynamic model of scraper conveyor sprocket transmission system can offer more veracious border condition for sprocket tooth profile optimization, structural strength evaluation and fatigue life prediction for key components.

79 p. This dissertation presents different ways to analysis chirp signals which are in heavy noise effectively, such as short-time Fourier transform (STFT), local polynomial Fourier transform (LPFT), and the bilinear Wigner-Ville distribution (WVD). The chirp signal also is called Linear Frequency-Modulated signal (LFM). There exist many real signals of chirp alike in the natural world to be analyzed. It has been applied widely in many fields of information system, such as communications, radar, sonar, earthquake recognition etc. So the high resolution of chirp signal analysis is required. Master of Science (Signal Processing)

To review the main achievement and problems of study on hand-arm vibration syndrome in China. The epidemiological and clinical study indicate that HAVS was reported from almost provinces in China, the prevalence of VWF ranges from 2.5% to 82.8% in the workers with vibrating tool use. The exposure-response relationship between prevalence of VWF and intensity and duration of exposed to hand-transmitted vibration has confirmed. Diagnostic criteria of HAVS has been established and performed by Chinese government in 1985, and it was revised by government as a national standard for occupational health in 2002. The "hygienic standard for hand-transmitted vibration in workplace" as a national standard and the "methods of measurement and assessment for hand-transmitted vibration" as a recommend standard were published by government also. The limited value of exposed to hand-arm vibration was 5.0 m/s2 that is energy equivalent frequency-weighted acceleration for a period of 4 h (ahw(4)). There are some problems in this field of China need to further study in the future.