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Additional file 1: Figure S1. XPS spectra of the ACaT samples. Figure S2. In vitro THZ1 release curves in PBS with different pH values. Figure S3. Transcriptome sequencing and bioinformatics analyses for ovarian cancer cells SKOV3 treated with PBS (control), THZ1 (0.5 ��M), NaALN (850 ��M) and ACaT (100 mg/L) for 24 h. Figure S4. KEGG pathway analysis of downregulated genes in SKOV3 cells treated with ACaT (100 mg/L) for 24 h. Figure S5. KEGG Focal Adhesion signaling pathway (04510) of SKOV3 cells treated with ACaT (100 mg/L) for 24 h. Figure S6. Cell cycle analysis of SKOV3 cells treated with PBS (control), NaALN (400 ��M), THZ1 (0.25 ��M), ACaT (12.5 mg/L) for 24 h by flow cytometry. Figure S7. ROS generation analysis of SKOV3 cells treated with THZ1 at concentrations of 0, 0.25, 0.5 and 1 ��M for 24 h by flow cytometry. Figure S8. The intracellular calcium ions assay of SKOV3 cells treated with PBS (control), NaALN (200 ��M), THZ1 (0.01 ��M) and ACaT (1.56 mg/L) for 48 h. Scale bar, 100 ��m. Figure S9. The mitochondrial membrane potential analyses of SKOV3 cells treated with PBS (control), NaALN (850 ��M), THZ1 (0.5 ��M) and ACaT (100 mg/L) for 6 h by flow cytometry using JC-1 staining. Figure S10. Cell viability analysis of SKOV3 cells treated with CaCl2 at different concentrations for 24 and 48 h. Figure S11. Quantitative data from scratch assay of SKOV3 cells treated with PBS (control), NaALN (200 ��M), THZ1 (0.01 ��M) and ACaT (6.25 mg/L) for 24 h. The wound gap% were plotted by GraphPad Prism 8.0. Figure S12. Construction of intraperitoneally disseminated ovarian tumor xenograft model on mouse. Figure S13. Anatomical bioluminescent images and bright fields of liver and spleen metastases (Arrows indicate the tumors). Figure S14. Fluorescent photographs of organs and tumors of dissected mice at 72 h post-injection. Figure S15. Abdominal girth changes of SKOV3 tumor-bearing mice in different treatment groups (n = 5). Figure S16. Body weight changes of SKOV3 tumor-bearing mice in different treatment groups (n = 5).