1. Ultrastructural analysis of collagen fibril diameter distribution in cleft lip
- Author
-
Maria Costanza Meazzini, Julian Little, Anuraag Singh, Tapas Chandra Nag, Luca Autelitano, Mohammad Faisal J. Khan, Peter A. Mossey, Ahmed Merajuddin, and Michele Rubini
- Subjects
Cleft Lip ,cleft lip ,cleft lip and palate ,collagen fibrils-edge diameter ,collagen number density ,fibril-area-fraction ,ImageJ ,Socio-culturale ,Collagen fibril ,03 medical and health sciences ,chemistry.chemical_compound ,Economica ,0302 clinical medicine ,Microscopy, Electron, Transmission ,Tissue biomechanics ,Humans ,Distribution (pharmacology) ,General Dentistry ,Medial side ,Ambientale ,030206 dentistry ,Anatomy ,Lateral side ,Extracellular Matrix ,Otorhinolaryngology ,Osmium tetroxide ,chemistry ,030220 oncology & carcinogenesis ,Ultrastructure ,Collagen ,Glutaraldehyde - Abstract
OBJECTIVE A preliminary study to determine collagen fibril diameter (CF-ED) distribution on medial and lateral sides of cleft lip (CL). MATERIAL AND METHODS Tissue samples from medial and lateral sides of CL were fixed in 2.5% glutaraldehyde and 1% osmium tetroxide and embedded in Araldite CY212 resin for transmission electron microscopy. The analysis of CF-ED was performed using the ImageJ program. To characterize the packaging of collagen fibrils (CFs) in the two tissues, we estimated the collagen number density (CF-ND) and fibril-area-fraction (FAF). Differences in measurements across the two sides were calculated using Wilcoxon signed-rank test. RESULTS The CF-ED was statistically significantly (p
- Published
- 2018
- Full Text
- View/download PDF