1. Genetic polymorphism in the adiponectin (ADIPOQ) gene and its association with the production and reproduction traits of Indian dairy cattle
- Author
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Satish K. Garg, Satyendra Pal Singh, Madhu Tiwari, Vijay Pandey, Rajesh Nigam, and Deepak Sharma
- Subjects
Genetics ,General Veterinary ,Adiponectin ,Genotype ,ADIPOQ gene ,PCR-RFLP ,SNP ,reproduction traits ,production traits ,cattle ,Single-nucleotide polymorphism ,Biology ,Allele ,ADIPOQ Gene ,Gene ,Allele frequency ,Dairy cattle ,gen ADIPOQ ,reproduktivna svojstva ,proizvodna svojstva ,govedo - Abstract
The present investigation was performed to explore the SNP in the promoter of the adiponectin (ADIPOQ) gene and its association with production and reproduction traits in Indian Sahiwal cows. A portion of the promoter (ADIPOQ) region of the ADIPOQ gene was amplified which revealed a 977 bp amplicon, and its PCR-RFLP assay with the restriction enzyme TasI revealed three genotypes, in which the CT genotype was the most frequent (62.32%), followed by CC (24.64%) and TT (13.04%). The frequency of the C and T alleles was 0.558 and 0.442, respectively. The ADIPOQ/TasI genotypes revealed a significant association with calving interval (CI) in the first and fourth lactations, in which the CC genotype showed a significantly (P, Cilj ovoga rada bio je istražiti polimorfizam pojedinačnog nukleotida (SNP) u promotoru gena za adiponektin (ADIPOQ) i njegovu povezanost s proizvodnim i rasplodnim svojstvima indijskog goveda Sahiwal pasmine. Dio promotora (ADIPOQ) regije gena ADIPOQ je umnožen i prikazan produktom 977 bp, a PCR-RFLP metodom s restrikcijom enzimom TasI dobivena su tri genotipa. Među njima najčešći je bio CT genotip (62,32 %), zatim CC (24,64 %) i TT (13,04 %). Učestalost C-alela bila je 0,558, a T-alela 0,442. ADIPOQ/TasI genotipovi su pokazali znakovitu povezanost s međutelidbenim intervalom (CI) u prvoj i četvrtoj laktaciji u kojima je CC genotip pokazao znakovito duži interval između teljenja (P
- Published
- 2020
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