1. Polyethylenimine Increases Antibacterial Efficiency of Chlorophyllin
- Author
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Faheem Ahmad Akif, Mona Mahmoud, Binod Prasad, Peter Richter, Azizullah Azizullah, Muhammad Qasim, Muhammad Anees, Marcus Krüger, Susanne Gastiger, Andreas Burkovski, Sebastian M. Strauch, and Michael Lebert
- Subjects
Microbiology (medical) ,Infectious Diseases ,chlorophyllin ,polyethlyenimine ,synergistic effect ,photodynamic antimicrobial activity ,ddc:570 ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,Biochemistry ,Microbiology - Abstract
Polyethylenimines (PEIs), a group of polycationic molecules, are known to impair the outer membrane of Gram-negative bacteria and exhibit antimicrobial activity. The outer membrane of Gram-negative strains hinders the uptake of photosensitizer chlorophyllin. In this study, we report chlorophyllin and branched PEI combinations’ activity against Escherichia coli strains DH5α and RB791, Salmonella enterica sv. Typhimurium LT2, and Bacillus subtilis 168. The minimal bactericidal concentration (MBC) was determined by plating cells treated with different concentrations of PEI and chlorophyllin on agar and monitoring their growth after 24 h. All tested combinations of PEI and chlorophyllin were lethal for S. enterica after 240 min of incubation in light, whereas PEI alone (
- Published
- 2022