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Today, the paper industry is faced with a global deficiency of raw wood materials, so alternative sources of virgin cellulose fibres are playing an important role in paper production. Agricultural countries produce large quantities of crop farming by-products such as straw, which is an interesting alternative raw material for cellulose fibres. Straw is used in many industries because of its numerous advantages: animal food industry, biofuel industry, construction industry and as artistic material. The potential use of straw production residues is of great importance in paper and printing industry. The focus of this research is on triticale straw, which was used as a non-wood fibre source for paper production. Namely, triticale straw was converted into semi-chemical pulp and was combined with recycled wood pulp in order to produce alternative laboratory papers. The usability of this kind of laboratory papers in printing industry was analysed based on line reproduction quality. This research evaluated and analysed line reproduction quality based on four line attributes: width, blurriness and raggedness. The results of this research proved that triticale pulp in laboratory papers has equal influence on line printing quality as the recycled wood pulp.