1. A rapid diagnostic multiplex PCR approach for xenomonitoring of human and animal schistosomiasis in a 'One Health' context
- Author
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Cyril Hammoud, Stephen Mulero, Aspire Mudavanhu, Ruben Schols, Tine Huyse, Hans Carolus, Laboratory of Biodiversity and Evolutionary Genomics, Catholic University of Leuven - Katholieke Universiteit Leuven (KU Leuven), Royal Museum for Central Africa [Tervuren] (RMCA), Ghenth University, Interactions Hôtes-Pathogènes-Environnements (IHPE), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD), and University of Zimbabwe (UZ)
- Subjects
0301 basic medicine ,HOST ,law.invention ,South Africa ,0302 clinical medicine ,law ,Schistosomiasis ,rapid diagnostic multiplex PCR ,Polymerase chain reaction ,Public, Environmental & Occupational Health ,Schistosoma haematobium ,gastropod-borne disease ,biology ,[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] ,General Medicine ,PRIMERS ,3. Good health ,Infectious Diseases ,Schistosoma ,Schistosoma mansoni ,Trematoda ,Life Sciences & Biomedicine ,RNA, Protozoan ,Zimbabwe ,Parasitic Diseases, Animal ,030231 tropical medicine ,Context (language use) ,Sensitivity and Specificity ,03 medical and health sciences ,Species Specificity ,PEOPLE ,Tropical Medicine ,Multiplex polymerase chain reaction ,parasitic diseases ,medicine ,Animals ,Humans ,MEDIATED ISOTHERMAL AMPLIFICATION ,One Health ,PARASITE ,Science & Technology ,Public Health, Environmental and Occupational Health ,Genetic Variation ,medicine.disease ,biology.organism_classification ,Virology ,GENE ,030104 developmental biology ,transmission monitoring ,Parasitology ,Multiplex Polymerase Chain Reaction - Abstract
Studying the epidemiology of schistosomiasis-the most prevalent gastropod-borne human disease and an economic burden for the livestock industry-relies on adequate monitoring tools. Here we describe a molecular assay for detecting human and animal African schistosome species in their planorbid gastropod host (xenomonitoring) using a two-step approach. First, schistosome infections are detected and discriminated from other trematode infections using a multiplex polymerase chain reaction (PCR) that includes a trematode-specific marker (in 18S rDNA), a Schistosoma genus-specific marker (in internal transcribed spacer 2 [ITS2]) and a general gastropod marker (in 18S rDNA) as an internal control. Upon Schistosoma sp. detection, a second multiplex PCR is performed to discriminate among Schistosoma haematobium, Schistosoma mansoni, Schistosoma mattheei and Schistosoma bovis/Schistosoma curassoni/Schistosoma guineensis using markers of differential lengths in the cytochrome c oxidase subunit 1 (COX1) gene. The specificity of these assays was validated with adult worms, naturally infected gastropods and human urine and stool samples. Sensitivity was tested on experimentally infected snail specimens that were sacrificed 10 and 40 days post-infection in order to mimic a natural prepatent and mature infection, respectively. The assay provides a diagnostic tool to support the xenomonitoring of planorbid gastropods for trematode infections in a One Health context, with a focus on the transmission monitoring of schistosomiasis. ispartof: TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE vol:113 issue:11 pages:722-729 ispartof: location:England status: published
- Published
- 2019
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