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Nitrogen removal with biological methods plays a crucial role in wastewater treatment technology. The treatment begins with the oxidation of ammonia to nitrite to facilitate the subsequent nitrification and denitrification. Various strains of ammonia-oxidising bacteria have been reported. In this study, we use three Bacillus bacteria isolated from swine wastewater to oxidise ammonia. Different initial densities (103, 104, 105, and 106 CFU·mL–1) of each strain were examined. The results show that the combination of all the bacteria at a ratio of 1:1:1 and a density of 105 CFU·mL–1 exhibits the most effect. The findings contribute to the diversity of ammonia-oxidising bacterial species and pose a great potential for applying these strains in wastewater treatment.

In nitrogen treatment with biological methods, nitrite metabolism is an intermediate process that facilitates other processes involving different bacteria strains. In this study, we isolated two nitrite-oxidising bacteria strains from abattoir wastewater and wastewater from biogas tanks of an industrial pig farm in Ha Tinh province. The bacteria strains grow, develop, and metabolise nitrite at pH 6–8 and 30–37 °C. The samples with the nitrite concentration up to 750 mg·L–1 were oxidised within four days of incubation, and the nitrite metabolism rate was proportional to the concentration of nitrite tested. Under severe conditions (salinity up to 3% NaCl, a low dissolved oxygen level of 0.1 mg·L–1), the two isolated bacterial strains exhibited their effective growth and nitrite metabolism capacity. The results enrich the database of nitrite-oxidising bacteria and are prospective in wastewater treatment.