Search

Your search keyword '"Gu, Yang"' showing total 1,571 results
Start Over Author "Gu, Yang" Publication Type Academic Journals Publication Type Magazines Publication Type Periodicals
1,571 results on '"Gu, Yang"'

8. Arterial chemotherapy for hepatocellular carcinoma in China: consensus recommendations

Author

Zhao, Ming, Guo, Zhi, Zou, Ying-Hua, Li, Xiao, Yan, Zhi-Ping, Chen, Min-Shan, Fan, Wei-Jun, Li, Hai-Liang, Yang, Ji-Jin, Chen, Xiao-Ming, Xu, Lin-Feng, Zhang, Yue-Wei, Zhu, Kang-Shun, Sun, Jun-Hui, Li, Jia-Ping, Jin, Yong, Yu, Hai-Peng, Duan, Feng, Xiong, Bin, Yin, Guo-Wen, Lin, Hai-Lan, Ma, Yi-Long, Wang, Hua-Ming, Gu, Shan-Zhi, Si, Tong-Guo, Wang, Xiao-Dong, Zhao, Chang, Yu, Wen-Chang, Guo, Jian-Hai, Zhai, Jian, Huang, Yong-Hui, Wang, Wei-Yu, Lin, Hai-Feng, Gu, Yang-Kui, Chen, Jin-Zhang, Wang, Jian-Peng, Zhang, Yi-Min, Yi, Jun-Zhe, and Lyu, Ning

Published
2024
Full Text
View/download PDF

18. Evolutionary route of nasopharyngeal carcinoma metastasis and its clinical significance

Author

Lin, Mei, Zhang, Xiao-Long, You, Rui, Liu, You-Ping, Cai, Hong-Min, Liu, Li-Zhi, Liu, Xue-Fei, Zou, Xiong, Xie, Yu-Long, Zou, Ru-Hai, Zhang, Yi-Nuan, Sun, Rui, Feng, Wei-Yi, Wang, Hai-Yan, Tao, Gui-Hua, Li, Hao-Jiang, Huang, Wen-Jie, Zhang, Chao, Huang, Pei-Yu, Wang, Jin, Zhao, Qi, Yang, Qi, Zhang, Hong-Wan, Liu, Ting, Li, Hui-Feng, Jiang, Xiao-Bing, Tang, Jun, Gu, Yang-Kui, Yu, Tao, Wang, Zhi-Qiang, Feng, Lin, Kang, Tie-Bang, Zuo, Zhi-Xiang, and Chen, Ming-Yuan

Published
2023
Full Text
View/download PDF

38. Directed Evolution of Artificial Metalloenzymes in Whole Cells

Author

Gu, Yang, Bloomer, Brandon J, Liu, Zhennan, Chen, Reichi, Clark, Douglas S, and Hartwig, John F

Subjects
Biotechnology, Rare Diseases, Metalloproteins, artificial metalloenzymes, directed evolution, N-H insertion, P450, whole cell, N−H insertion, Chemical Sciences, Organic Chemistry
Abstract

Artificial metalloenzymes (ArMs), created by introducing synthetic cofactors into protein scaffolds, are an emerging class of catalyst for non-natural reactions. Although many classes of ArMs are known, in vitro reconstitution of cofactors and proteins has been a limiting step in the high-throughput screening and directed evolution of ArMs because purification of individual host proteins is time-consuming. We describe the application of a platform to combine mutants of the P450 enzyme CYP119 and the cofactor Ir(Me)MPIX in vivo, by coexpression of the CYP119 mutants with the heme transporter encoded by the hug operon, to the directed evolution of ArMs containing Ir(Me)MPIX in whole cells. We applied this platform to the development an ArMs catalyzing the insertion of the acyclic carbene from α-diazopropanoate esters (Me-EDA) into the N-H bonds of N-alkyl anilines, a combination of carbene and amine classes for which mutant enzymes of natural hemoproteins previously reacted with low enantioselectivity. The mutants of the artificial metalloenzyme Ir(Me)CYP119 identified by an evolution campaign involving more than 4000 mutants are shown to catalyze the reaction of Me-EDA with N-methyl anilines to form chiral chiral amino esters with high TON and good enantioselectivity, thereby demonstrating that the directed evolution of ArMs can rival that of natural enzymes in vivo.

Published
2022

39. Assembly and Evolution of Artificial Metalloenzymes within E. coli Nissle 1917 for Enantioselective and Site-Selective Functionalization of CH and CC Bonds

Author

Liu, Zhennan, Huang, Jing, Gu, Yang, Clark, Douglas S, Mukhopadhyay, Aindrila, Keasling, Jay D, and Hartwig, John F

Subjects
Inorganic Chemistry, Chemical Sciences, Biotechnology, Carbon, Catalysis, Cytochrome P-450 Enzyme System, Escherichia coli, Evolution, Molecular, Hydrogen, Iridium, Metalloproteins, Methane, Mutagenesis, Site-Directed, Porphyrins, Stereoisomerism, CSD-02-CAT-A, CSD-46-All CSGB, General Chemistry, Chemical sciences, Engineering
Abstract

The potential applications afforded by the generation and reactivity of artificial metalloenzymes (ArMs) in microorganisms are vast. We show that a non-pathogenic E. coli strain, Nissle 1917 (EcN), is a suitable host for the creation of ArMs from cytochrome P450s and artificial heme cofactors. An outer-membrane receptor in EcN transports an iridium porphyrin into the cell, and the Ir-CYP119 (CYP119 containing iridium porphyrin) assembled in vivo catalyzes carbene insertions into benzylic C-H bonds enantioselectively and site-selectively. The application of EcN as a whole-cell screening platform eliminates the need for laborious processing procedures, drastically increases the ease and throughput of screening, and accelerates the development of Ir-CYP119 with improved catalytic properties. Studies to identify the transport machinery suggest that a transporter different from the previously assumed ChuA receptor serves to usher the iridium porphyrin into the cytoplasm.

Published
2022

42. Ultrasound-based deep learning in the establishment of a breast lesion risk stratification system: a multicenter study

Author

Gu, Yang, Xu, Wen, Liu, Ting, An, Xing, Tian, Jiawei, Ran, Haitao, Ren, Weidong, Chang, Cai, Yuan, Jianjun, Kang, Chunsong, Deng, Youbin, Wang, Hui, Luo, Baoming, Guo, Shenglan, Zhou, Qi, Xue, Ensheng, Zhan, Weiwei, Zhou, Qing, Li, Jie, Zhou, Ping, Chen, Man, Gu, Ying, Chen, Wu, Zhang, Yuhong, Li, Jianchu, Cong, Longfei, Zhu, Lei, Wang, Hongyan, and Jiang, Yuxin

Published
2023
Full Text
View/download PDF

50. Effect of laparoscopic side-to-side jejunoileal anastomosis on glucose and lipid metabolism in type 2 diabetes mellitus

Author

WANG Zhonghui, LYU Hongliang, SUN Tieliang, DAI Chunlei, YANG Jupeng, LIU Ming, GU Yang, ZHANG Hongyi, JIN Hu

Subjects
side-to-side jejunoileal anastomosis, type 2 diabetes mellitus, blood glucose, blood lipid, total cholesterol, triacylglycerol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, glycosylated hemoglobin, Medicine
Abstract

Objective To analyze the effect of laparoscopic side-to-side jejunoileal anastomosis on blood glucose and lipid metabolism in type 2 diabetes mellitus（T2DM） patients. Methods The clinical data of 52 patients with T2DM diagnosed in Jilin Province FAW Hospital from January 2018 to February 2019 who underwent laparoscopic side-to-side jejunoileal anastomosis were retrospectively analyzed. The changes of total cholesterol（TC）, triacylglycerol（TG）, high-density lipoprotein cholesterol（HDL-C）, low-density lipoprotein cholesterol（LDL-C）, and glycosylated hemoglobin（HbA1c） before and 3 years after the operation were analyzed. Results Among the 52 patients, 23 had complete remission, 11 had partial remission, 8 had significant improvement, and 10 had no effect at 3 years after surgery. The overall effective rate of blood glucose control was 80.77%. In the control goal of preventing atherosclerosis cardiovascular disease（ASCVD） high-risk groups in T2DM patients, the rate of reaching the target of LDL-C＜2.6mmol/L（74.19% vs 12.90%） and TG＜1.7mmol/L（77.42% vs 48.39%） after surgery were significantly higher than those before surgery（P

Published
2023
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results