7 results on '"Zelentsova, Ekaterina A."'
Search Results
2. Animal Metabolite Database: Metabolite Concentrations in Animal Tissues and Convenient Comparison of Quantitative Metabolomic Data.
- Author
-
Yanshole, Vadim V., Melnikov, Arsenty D., Yanshole, Lyudmila V., Zelentsova, Ekaterina A., Snytnikova, Olga A., Osik, Nataliya A., Fomenko, Maxim V., Savina, Ekaterina D., Kalinina, Anastasia V., Sharshov, Kirill A., Dubovitskiy, Nikita A., Kobtsev, Mikhail S., Zaikovskii, Anatolii A., Mariasina, Sofia S., and Tsentalovich, Yuri P.
- Subjects
DATABASES ,METABOLOMICS ,CRYSTALLINE lens ,LABORATORY rodents ,TISSUES ,HEART - Abstract
The Animal Metabolite Database (AMDB, https://amdb.online) is a freely accessible database with built-in statistical analysis tools, allowing one to browse and compare quantitative metabolomics data and raw NMR and MS data, as well as sample metadata, with a focus on the metabolite concentrations rather than on the raw data itself. AMDB also functions as a platform for the metabolomics community, providing convenient deposition and exchange of quantitative metabolomic data. To date, the majority of the data in AMDB relate to the metabolite content of the eye lens and blood of vertebrates, primarily wild species from Siberia, Russia and laboratory rodents. However, data on other tissues (muscle, heart, liver, brain, and more) are also present, and the list of species and tissues is constantly growing. Typically, every sample in AMDB contains concentrations of 60–90 of the most abundant metabolites, provided in nanomoles per gram of wet tissue weight (nmol/g). We believe that AMDB will become a widely used tool in the community, as typical metabolite baseline concentrations in tissues of animal models will aid in a wide variety of fundamental and applied scientific fields, including, but not limited to, animal modeling of human diseases, assessment of medical formulations, and evolutionary and environmental studies. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
3. Quantitative Metabolomic Dataset of Avian Eye Lenses.
- Author
-
Zelentsova, Ekaterina A., Mariasina, Sofia S., Yanshole, Vadim V., Yanshole, Lyudmila V., Osik, Nataliya A., Sharshov, Kirill A., and Tsentalovich, Yuri P.
- Subjects
CRYSTALLINE lens ,BIOLOGICAL systems ,METABOLOMICS ,PLANT breeding ,ANIMAL diversity - Abstract
Metabolomics is a powerful set of methods that uses analytical techniques to identify and quantify metabolites in biological samples, providing a snapshot of the metabolic state of a biological system. In medicine, metabolomics may help to reveal the molecular basis of a disease, make a diagnosis, and monitor treatment responses, while in agriculture, it can improve crop yields and plant breeding. However, animal metabolomics faces several challenges due to the complexity and diversity of animal metabolomes, the lack of standardized protocols, and the difficulty in interpreting metabolomic data. The current dataset includes quantitative metabolomic profiles of eye lenses from 26 bird species (111 specimens) that can aid researchers in developing new experiments, mathematical models, and integrating with other "-omics" data. The dataset includes raw
1 H NMR spectra, protocols for sample preparation, and data preprocessing, with the final table containing information on the abundance of 89 reliably identified and quantified metabolites. The dataset is quantitative, making it relevant for supplementing with new specimens or comparison groups, followed by data mining and expected new interpretations. The data were obtained using the bird specimens collected in compliance with ethical standards and revealed potential differences in metabolic pathways due to phylogenetic differences or environmental exposure. Dataset: The dataset, including NMR raw data, metabolite concentrations, and experimental protocols, has been deposited in the MetaboLights repository, study identifier MTBLS7739 (https://www.ebi.ac.uk/metabolights/MTBLS7739 (accessed on 30 June 2023)). DOI: 10.1093/nar/gkz1019, PMID: 31691833. This dataset is now also available through the laboratory's own database, accessible via the following links: amdb.online/amdb/experiments/145/; amdb.online/amdb/experiments/199/; amdb.online/amdb/experiments/255/; amdb.online/amdb/experiments/248/. Dataset License: CC0 [ABSTRACT FROM AUTHOR]- Published
- 2023
- Full Text
- View/download PDF
4. Influence of Ecological Factors on the Metabolomic Composition of Fish Lenses.
- Author
-
Tsentalovich, Yuri P., Zelentsova, Ekaterina A., Savina, Ekaterina D., Yanshole, Vadim V., and Sagdeev, Renad Z.
- Subjects
- *
METABOLOMICS , *ORGANIC acids , *WATER acidification , *WATER pollution , *ROACH (Fish) , *EUROPEAN perch , *FISHERY products , *FISH meal - Abstract
Simple Summary: Biological responses to rugged ecological factors causes changes in the metabolic pathways in aquatic organisms, which may manifest as changes in the tissue metabolomic composition. Therefore, establishing a correlation between the metabolite concentrations in aquatic animals and ecological factors is important for understanding the molecular mechanisms of biological responses to ecological stresses. In this work, we determined the concentrations of 57 major metabolites in the lenses of fish from three locations in Siberia (Russia) that differed in levels of dissolved oxygen (LDO) and water purity. We found that the increased due to CO2 emissions water acidity and the reduced LDO caused significant changes in the fish lenses' metabolomic compositions, including amino acids, organic acids, and energy metabolites. The obtained results can be used in monitoring the ecological state of water bodies. Multiple stressors related to changes in environmental conditions (such as water temperature, salinity, and natural and anthropogenic pollution) may cause biological responses of aquatic organisms that lead to significant variations in the biochemical reactions in their tissues and thereby change the concentrations of metabolites. We used a quantitative NMR-based metabolomic analysis of the fish lens for the evaluation of the influence of environmental factors on metabolic processes in aquatic animals. For this purpose, three species of freshwater fish—Perca fluviatilis, Rutilus rutilus lacustris, and Gymnocephalus cernua—were caught at approximately the same time at three locations in Siberia (Russia) that differed in levels of dissolved oxygen (LDO) and water purity, and the concentrations of 57 major metabolites in the fish lenses were determined. We found that the metabolomic profiles of the fish lenses strongly depended on the location. The obtained data demonstrated that two typical stressors for aquatic animals—a reduced LDO and anthropogenic water pollution—caused a largely similar metabolic response in the fish lenses that led to an increase in the concentrations of several amino acids and a decrease in sarcosine and phosphoethanolamine. At the same time, the composition of the major lens osmolytes depended mostly on the oxygen level, while variations in AMP (decrease) and NAD (increase) corresponded to the water pollution. We suggest that the eye lens is a very convenient tissue for studying the impact of ecological factors on the metabolic state of aquatic animals, fish in particular. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
5. The Application of Quantitative Metabolomics for the Taxonomic Differentiation of Birds.
- Author
-
Zelentsova, Ekaterina A., Yanshole, Lyudmila V., Tsentalovich, Yuri P., Sharshov, Kirill A., and Yanshole, Vadim V.
- Subjects
- *
CROWS , *RNA sequencing , *CORVUS corax , *HIERARCHICAL clustering (Cluster analysis) , *ENGLISH sparrow , *GREAT tit - Abstract
Simple Summary: Modern evolutionary biology offers a wide variety of methods to explore the evolution of species and to describe their relationships. The methods of DNA/RNA sequence analysis have been developing for decades and have become increasingly popular and reasonably reliable. Nevertheless, final phylogenetic trees for many taxa are still under debate because both classical and genomics-based approaches have their own limitations for phylogenetic tree reconstruction. Here, we propose the use of younger 'omics' methods, namely quantitative metabolomics, to aid the phylogeny reconstruction of vertebrates. We show that metabolomics-based hierarchical clustering analysis trees match, although not perfectly, to the genomics-based trees. In the current pilot study, we propose the use of quantitative metabolomics to reconstruct the phylogeny of vertebrates, namely birds. We determined the concentrations of the 67 most abundant metabolites in the eye lenses of the following 14 species from 6 orders of the class Aves (Birds): the Black kite (Milvus migrans), Eurasian magpie (Pica pica), Northern raven (Corvus corax), Eurasian coot (Fulica atra), Godlewski's bunting (Emberiza godlewskii), Great crested grebe (Podiceps cristatus), Great tit (Parus major), Hawfinch (Coccothraustes coccothraustes), Hooded crow (Corvus cornix), House sparrow (Passer domesticus), Rock dove (Columba livia), Rook (Corvus frugilegus), Short-eared owl (Asio flammeus) and Ural owl (Strix uralensis). Further analysis shows that the statistical approaches generally used in metabolomics can be applied for differentiation between species, and the most fruitful results were obtained with hierarchical clustering analysis (HCA). We observed the grouping of conspecific samples independently of the sampling place and date. The HCA tree structure supports the key role of genomics in the formation of the lens metabolome, but it also indicates the influence of the species lifestyle. A combination of genomics-based and metabolomics-based phylogeny could potentially resolve arising issues and yield a more reliable tree of life. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
6. Seasonal Variations and Interspecific Differences in Metabolomes of Freshwater Fish Tissues: Quantitative Metabolomic Profiles of Lenses and Gills.
- Author
-
Tsentalovich, Yuri P., Yanshole, Vadim V., Yanshole, Lyudmila V., Zelentsova, Ekaterina A., Melnikov, Arsenty D., and Sagdeev, Renad Z.
- Subjects
FRESHWATER fishes ,METABOLOMICS ,ROACH (Fish) ,FISH anatomy ,SEASONAL distribution of fishes ,GILLS ,PHYTIC acid ,ETHANOLAMINES - Abstract
This work represents the first comprehensive report on quantitative metabolomic composition of tissues of pike-perch (Sander lucioperca) and Siberian roach (Rutilus rutilus lacustris). The total of 68 most abundant metabolites are identified and quantified in the fish lenses and gills by the combination of LC-MS and NMR. It is shown that the concentrations of some compounds in the lens are much higher than that in the gills; that indicates the importance of these metabolites for the adaptation to the specific living conditions and maintaining the homeostasis of the fish lens. The lens metabolome undergoes significant seasonal changes due to the variations of dissolved oxygen level and fish feeding activity. The most season-affected metabolites are osmolytes and antioxidants, and the most affected metabolic pathway is the histidine pathway. In late autumn, the major lens osmolytes are N-acetyl-histidine and threonine phosphoethanolamine (Thr-PETA), while in winter the highest concentrations were observed for serine phosphoethanolamine (Ser-PETA) and myo-inositol. The presence of Thr-PETA and Ser-PETA in fish tissues and their role in cell osmotic protection are reported for the first time. The obtained concentrations can be used as baseline levels for studying the influence of environmental factors on fish health. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
7. Ovothiol A is the Main Antioxidant in Fish Lens.
- Author
-
Yanshole, Vadim V., Yanshole, Lyudmila V., Zelentsova, Ekaterina A., and Tsentalovich, Yuri P.
- Subjects
ROACH (Fish) ,FRESHWATER fishes ,OPTICAL spectroscopy ,FRESHWATER animals ,CELL nuclei ,NUCLEAR magnetic resonance spectroscopy - Abstract
Tissue protection from oxidative stress by antioxidants is of vital importance for cellular metabolism. The lens mostly consists of fiber cells lacking nuclei and organelles, having minimal metabolic activity; therefore, the defense of the lens tissue from the oxidative stress strongly relies on metabolites. Protein-free extracts from lenses and gills of freshwater fish, Sander lucioperca and Rutilus rutilus lacustris, were subjected to analysis using high-field
1 H NMR spectroscopy and HPLC with optical and high-resolution mass spectrometric detection. It was found that the eye lenses of freshwater fish contain high concentrations of ovothiol A (OSH), i.e., one of the most powerful antioxidants exciting in nature. OSH was identified and quantified in millimolar concentrations. The concentration of OSH in the lens and gills depends on the fish genus and on the season. A possible mechanism of the reactive oxygen species deactivation in fish lenses is discussed. This work is the first to report on the presence of OSH in vertebrates. The presence of ovothiol in the fish tissue implies that it may be a significantly more common antioxidant in freshwater and marine animals than was previously thought. [ABSTRACT FROM AUTHOR]- Published
- 2019
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.