Search

This paper presents a method for fitting digital hyperspheres to a given set of n D points in an image in the presence of noise by maximizing the number of inliers, namely the consensus set. The digital Hyperspheres are defined using the k -Flake Digitization models [25] . We present an algorithm, that provides optimal fitting solutions for digital k -Flake hyperspheres within a time complexity O ( ( ( n k ) 2 n − k ) n N n + 1 log ⁡ N ) for dimension n , N being the number of points. We have implemented this algorithm for the particular case of 3D 2-Flake spheres that corresponds to the classical so called Naive digital spheres. [ABSTRACT FROM AUTHOR]

We investigated the commercially available Episkin LM™ reconstructed epidermis test system as a potential 3D model for human genotoxicity assessment by cytokinesis-block micronucleus assay to mitigate limitations of the currently accepted micronucleus test. We established appropriate culture conditions for cytokinesis-block micronucleus assay in maximizing the frequency of binucleated cells by choice of culture medium and calibration of the system exposure to the cytokinesis inhibitor Cytochalasin B, without affecting the basal frequency of micronuclei in the model. We confirmed that the application of the classic solvents had no significant effect on this basal level of micronuclei. We determined the performance of cytokinesis-block micronucleus assay in Episkin LM™ reconstructed epidermis to predict in vivo genotoxins by testing the genotoxicity potential of 17 well known in vivo genotoxic, progenotoxic and non-genotoxic reference chemicals over a 48 h and 72 h exposure period. We found that cytokinesis-block micronucleus assays in Episkin™ reconstructed epidermis following the 48 h-topical regimen had a specificity of 60–75% and a sensitivity of 83–85%, resulting in an overall accuracy of 76–82% for genotoxicity assessment in tissues depending on the assessment of the reference chemicals with equivocal genotoxic profiles in the literature. The positive micronucleus test results obtained without addition of any exogenous metabolic activation system confirmed the ability of Episkin LM™ reconstructed epidermis to intrinsically bioactivate progenotoxic chemicals. The evidence showed that the 72-h exposure protocol significantly improved the detection of progenotoxins. Taken together, our data demonstrated that the Episkin LM™ reconstructed epidermis system is a relevant in vitro tool in the study of genetic toxicology. [ABSTRACT FROM AUTHOR]