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Bacterial cellulose (BC) hydrogel is renowned in the field of tissue engineering for its high biocompatibility, excellent mechanical strength, and eco-friendliness. Herein, we present a biomimetic mineralization method for preparing BC/hydroxyapatite (HAP) composite hydrogel scaffolds with different mineralization time and ion concentration of the mineralized solution. Spherical HAP reinforcement enhanced bone mineralization, thereby imparting increased bioactivity to BC matrix materials. Subsequently, platelet-rich plasma (PRP) was introduced into the scaffold. The PRP-loaded hydrogel enhanced the release of growth factors, which promoted cell adhesion, growth, and bone healing. After 3 weeks of MC3T3-E1 cell-induced osteogenesis, PRP positively affected cell differentiation in BC/HAP@PRP scaffolds. Overall, these scaffolds exhibited excellent biocompatibility, mineralized nodule formation, and controlled release in vitro , demonstrating great potential for application in bone tissue repair. [ABSTRACT FROM AUTHOR]

Bacterial cellulose, as a kind of natural biopolymer produced by bacterial fermentation, has attracted wide attention owing its unique physical and chemical properties. Nevertheless, the single functional group on the surface of BC greatly hinders its wider application. The functionalization of BC is of great significance to broaden the application of BC. In this work, N -acetylated bacterial cellulose (ABC) was successfully prepared using K. nataicola RZS01-based direct synthetic method. FT-IR, NMR and XPS confirmed the in-situ modification of BC by acetylation. The SEM and XRD results demonstrated that ABC has a lower crystallinity and higher fiber width compare with pristine 88 BCE % cell viability on NIH-3 T3 cell and near zero hemolysis ratio indicate its good biocompatibility. In addition, the as-prepared acetyl amine modified BC was further treated by nitrifying bacteria to enrich its functionalized diversity. This study provides a mild in-situ pathway to construct BC derivatives in an environmentally friendly way during its metabolism. We proposed an in-situ approach for the biosynthesis of amidated BC, by using D-glucosamine as substrates in microbial synthesis system. This finding provides a mild route to in situ construct amidated BC in a friendly and environmentally way during its metabolic process. [Display omitted] • Aminated-BC can be obtained by in situ microbial fermentation of K. nataicola RZS01 culturing with glucosamine. • The as-prepared Aminated-BC shows lower crystallinity and higher fiber width. • The as-prepared Aminated-BC shows good biocompatibility and can be further modified to enrich its functionalized diversity. [ABSTRACT FROM AUTHOR]

Bacterial cellulose, as a kind of natural biopolymer produced by bacterial fermentation, has attracted wide attention owing its unique physical and chemical properties. Nevertheless, the single functional group on the surface of BC greatly hinders its wider application. The functionalization of BC is of great significance to broaden the application of BC. In this work, N -acetylated bacterial cellulose (ABC) was successfully prepared using K. nataicola RZS01-based direct synthetic method. FT-IR, NMR and XPS confirmed the in-situ modification of BC by acetylation. The SEM and XRD results demonstrated that ABC has a lower crystallinity and higher fiber width compare with pristine 88 BCE % cell viability on NIH-3 T3 cell and near zero hemolysis ratio indicate its good biocompatibility. In addition, the as-prepared acetyl amine modified BC was further treated by nitrifying bacteria to enrich its functionalized diversity. This study provides a mild in-situ pathway to construct BC derivatives in an environmentally friendly way during its metabolism. We proposed an in-situ approach for the biosynthesis of amidated BC, by using D-glucosamine as substrates in microbial synthesis system. This finding provides a mild route to in situ construct amidated BC in a friendly and environmentally way during its metabolic process. [Display omitted] • Aminated-BC can be obtained by in situ microbial fermentation of K. nataicola RZS01 culturing with glucosamine. • The as-prepared Aminated-BC shows lower crystallinity and higher fiber width. • The as-prepared Aminated-BC shows good biocompatibility and can be further modified to enrich its functionalized diversity. [ABSTRACT FROM AUTHOR]

Air pollution has become a major public health concern, attracting considerable attention from researchers working on environmentally friendly and sustainable materials. In this work, bacterial cellulose (BC) derived aerogels were fabricated by the directional ice-templated method and used as filters to remove PM particles. We modified the surface functional groups of BC aerogel with reactive silane precursors, and investigated the interfacial and structural properties of those aerogels. The results show that BC-derived aerogels have excellent compressive elasticity, and their directional growth orientation inside the structure significantly reduced pressure drop. Moreover, the BC-derived filters exhibit an exceptional quantitative removal effect on fine particulate matter, which, in the presence of high concentrations of fine particulate matter, they can achieve a high-efficiency removal standard of 95 %. Meanwhile, the BC-derived aerogels showed superior biodegradation performance in the soil burial test. These results paved the way for BC-derived aerogels development as a great sustainable alternative to treat air pollution. [ABSTRACT FROM AUTHOR]

Bacterial cellulose/hydroxyapatite (BC/HAp) composite is an outstanding candidate for bone tissue engineering. The conventional biomimetic mineralization method takes a long time with unsatisfactory mechanical properties and biocompatibility. Herein, we modified the BC by changing the carbon source to calcium gluconate during the biosynthesis process of BC by bacteria, providing nucleation sites for further mineralization in simulated body fluid. Results show spherical porous HAp in the size of 100–200 nm was fully filled in the three-dimensional network structure of BC nanofibers uniformly within five days of mineralization. Molecular dynamics simulation shows that the aggregation of cellulose units in aqueous solution can enhance the adsorption of calcium ions. By this means, we significantly improved the mechanical properties and biocompatibility of the BC/HAp composite, as well as simplified the preparation process, compared to conventional method, which, therefore, suggests, it could be further studied for biomedical applications such as bone tissue engineering. We modified the bacterial cellulose with calcium ions by changing the carbon source to calcium gluconate during the biosynthesis process of BC, which provides nucleation sites for further mineralization of hydroxyapatite. This method significantly improves the mechanical properties and biocompatibility of the BC/HAp composite compared to those made from conventional method, which, therefore, suggests it could be further studied for biomedical applications such as bone tissue engineering. [Display omitted] [ABSTRACT FROM AUTHOR]