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The paper publishes the first records of the occurrence of a native species Sonchus palustris and three alien species Eryngium giganteum, Inula ensifolia and Medicago orbicularis in South Bohemia. New localities of rare native or rare alien taxa and threatened or otherwise important taxa of the South Bohemian flora are reported and described: Carex otrubae, Ceratophyllum submersum, Cynodon dactylon, Dipsacus laciniatus, Erysimum cheiri, Erucastrum gallicum, Geranium macrorrhizum, Hieracium bifidum, Lathyrus nissolia subsp. pubescens, Rubus centrobohemicus, Sisymbrium orientale subsp. orientale, Sorghum bicolor, Thelypteris palustris and Trifolium fragiferum. For some of the species mentioned above, regional distribution or known localities are only updated or supplemented here. Particularly, the record of Verbena offi- cinalis near the village of Ratiborova Lhota in the Chvalšinské Předšumaví phytogeographical district was found to be wrong. [ABSTRACT FROM AUTHOR]