1. IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma.
- Author
-
Bergauer A, Sopel N, Kroß B, Vuorinen T, Xepapadaki P, Weiss ST, Blau A, Sharma H, Kraus C, Springel R, Rauh M, Mittler S, Graser A, Zimmermann T, Melichar VO, Kiefer A, Kowalski ML, Sobanska A, Jartti T, Lukkarinen H, Papadopoulos NG, and Finotto S
- Subjects
- Asthma virology, Cells, Cultured, Child, Preschool, Disease Progression, Europe, Female, Humans, Interferon Regulatory Factor-1 genetics, Interferons immunology, Male, Myeloid Differentiation Factor 88 genetics, Nasopharynx virology, Prospective Studies, RNA, Messenger analysis, Rhinovirus, STAT1 Transcription Factor genetics, STAT2 Transcription Factor genetics, Signal Transduction, Asthma immunology, C-Reactive Protein analysis, Interferons blood, Leukocytes, Mononuclear virology, Picornaviridae Infections immunology
- Abstract
We analysed the influence of rhinovirus (RV) in nasopharyngeal fluid (NPF) on type I and III interferon (IFN) responses (e.g. IFN-α and IFN -: λ) and their signal transduction, at baseline and during disease exacerbation, in cohorts of pre-school children with and without asthma.At the time of recruitment into the Europe-wide study PreDicta, and during symptoms, NPF was collected and the local RV colonisation was analysed. Peripheral blood mononuclear cells (PBMCs) were challenged in vitro with RV or not. RNA was analysed by quantitative real-time PCR and gene arrays. Serum was analysed with ELISA for IFNs and C-reactive protein.We found that PBMCs from asthmatic children infected in vitro with the RV1b serotype upregulated MYD88, IRF1, STAT1 and STAT2 mRNA, whereas MYD88, IRF1, STAT1 and IRF9 were predominantly induced in control children. Moreover, during symptomatic visits because of disease exacerbation associated with RV detection in NPF, IFN-α production was found increased, while IFN-λ secretion was already induced by RV in asthmatic children at baseline.During asthma exacerbations associated with RV, asthmatic children can induce IFN-α secretion, indicating a hyperactive immune response to repeated respiratory virus infection., (Copyright ©ERS 2017.)
- Published
- 2017
- Full Text
- View/download PDF