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Two obligately anaerobic, Gram-stain-negative, non-motile, non-spore-forming and short rod shaped bacteria, designated KGMB07931 T and KGMB10229, were isolated from faeces of two Korean persons. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains KGMB07931 T and KGMB10229 were very similar to each other (99.9%) and grouped within the genus Bacteroides, displaying the highest similarity with Bacteroides uniformis ATCC 8492 T (97.5%), Bacteroides rodentium JCM 16496 T (96.6%), and Bacteroides fluxus YIT 12057 T (94.5%). Both strains grew optimally at 37 °C and pH 7.5 in the presence of 0.5% (w/v) NaCl. The complete genome of KGMB07931 T comprises 3,335 protein-coding genes with a total of 4,240,638 bp and an average G + C content of 46.3 mol%. The major fatty acids were C 18:1  cis9, anteiso-C 15:0 , iso-C 15:0 , and Summed Feature 11 (iso-C 17:0 3OH and/or C 18:2 DMA); the predominant respiratory quinones were MK-9 and MK-10; the major fermentation end products were acetate and isobutyrate. The genome of strain KGMB07931 T encoded the starch utilization system (Sus) operon, susABCDEFG, suggesting that this strain uses many complex polysaccharides that cannot be digested by humans. Based on polyphasic taxonomic data, strains KGMB07931 T and KGMB10229 represent a novel species within the genus Bacteroides, for which the name Bacteroides humanifaecis sp. nov. is proposed. The type strain is KGMB07931 T (= KCTC 25160 T  = NBRC 115005 T )., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

A novel Gram-stain-negative and strictly anaerobic bacterial strain, designated KGMB02408 T , was isolated from faeces of a healthy human in the Republic of Korea. The isolate was characterized as non-motile, non-spore-forming and rod-shaped (variable in length). The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that strain KGMB02408 T belonged to the genus Bacteroides and was most closely related to Bacteroides faecichinchillae JCM 17102 T (=KCTC 15666 T ; 96.5 %). Based on its whole-genome sequence, the DNA G+C content of the isolate was 39.5 mol%. The average nucleotide identity value between strain KGMB02408 T and related species, B. faecichinchillae JCM 17102 T , was 93.8 %. The major cellular fatty acids (>10 %) of the isolate were anteiso-C 15 : 0 , iso-C 17 : 0 -OH, summed feature 11 (iso-C 17 : 0 -OH and/or C 18 : 2 DMA) and C 16 : 0 . Menaquinone-8 (28.6 %) and menaquinone-10 (47.1 %) were detected as the major respiratory quinones in the isolate. The major end products of glucose fermentation produced by strain KGMB02408 T were lactic acid, acetic acid and formic acid. Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, strain KGMB02408 T represents a novel species of the genus Bacteroides in the family Bacteroidaceae . The type strain is KGMB02408 T (=KCTC 15687 T =DSM 107828 T ).