1. Developing a novel strategy for fabricating matrix film to assess the distribution of potassium perfluorooctanic sulfonate by matrix-assisted laser desorption/ionization mass spectrometry imaging.
- Author
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Luo, Yake, Ma, Shanshan, Zhang, Jianxun, Zhang, Qidong, Zhang, Yanhao, Mao, Jian, Yuan, Hang, Ouyang, Gangfeng, Zhang, Shusheng, and Zhao, Wuduo
- Subjects
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MATRIX-assisted laser desorption-ionization , *MOTION picture distribution , *MASS spectrometry , *ADHESIVE tape , *POTASSIUM , *DESORPTION - Abstract
Matrix deposition plays a critical role in image quality of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI). To improve the ionization efficiency and overcome the limitation of traditional matrix deposition methods in the face of difficult-to-sublimate or difficult-to-dissolve matrix, covalent organic frameworks (COFs) named COF-DhaTab was successfully synthesized and firstly used as matrix film. It was fabricated by imprinting of sieved COF-DhaTab powder on the surface of a double-sided adhesive tape. Outstanding reproducibility and uniformity of COF-DhaTab film were demonstrated by relative standard deviation (RSD) within 8.37% and 7.71% from dot-to-dot and plate-to-plate, respectively. With the introduction of double-sided adhesive tape, water contact angle (WCA) of COF-DhaTab film increased from 55° to 141°, resulting in significant suppression of analyte diffusion. Moreover, the intensity of potassium perfluorooctanic sulfonate (PFOS, C 8 F 17 SO 3 −, m/z 498.93) was 9.3 × 105, more than six hundred times higher than that using DHB matrix. This enhancement was attributed to the rough surface and multiple branches of the synthesized COF-DhaTab. To verify the ability of COF-DhaTab film as substrate, the spatial distribution of PFOS in zebrafish, rat liver and kidney tissues was explored. Superior imaging capability was displayed with high-spatial resolution and reliable location distribution. These results not only demonstrate the outstanding ability of COF-DhaTab as matrix for MALDI-MS and MALDI-MSI, but also provide a facile approach for fabrication of novel matrix films for MALDI-MSI. [Display omitted] • A novel strategy was developed to fabricate matrix film for difficult-to-sublimate or difficult-to-dissolve matrix. • MALDI-MS signal of PFOS with COF-DhaTab matrix was increased by 600 times than DHB matrix. • Matrix film of sieved COF-DhaTab was used to study spatial distribution of PFOS by MALDI-MSI. • Excellent MALDI image quality was demonstrated by COF-DhaTab matrix film. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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