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Dwarf apple is one of the most important forms of garden economy, which has become a new engine for rural revitalization. The effective detection of coloring and ripening apples in complex environments is important for the sustainable development of smart agricultural operations. Addressing the issues of low detection efficiency in the greenhouse and the challenges associated with deploying complex target detection algorithms on low-cost equipment, we propose an enhanced lightweight model rooted in YOLOv7. Firstly, we enhance the model training performance by incorporating the Squeeze-and-Excite attention mechanism, which can enhance feature extraction capability. Then, an SCYLLA-IoU (SIoU) loss function is introduced to improve the ability of extracting occluded objects in complex environments. Finally, the model was simplified by introducing depthwise separable convolution and adding a ghost module after up-sampling layers. The improved YOLOv7 model has the highest AP value, which is 10.00%, 5.61%, and 6.00% higher compared to YOLOv5, YOLOv7, and YOLOX, respectively. The improved YOLOv7 model has an MAP value of 95.65%, which provides higher apple detection accuracy compared to other detection models and is suitable for potted dwarf anvil apple identification and detection. [ABSTRACT FROM AUTHOR]

Actinobacillus pleuropneumoniae is the causative agent of pleuropneumonia, an economically important lung disease in pigs. In draft genomes of two Cypriot clinical A. pleuropneumoniae isolates (MIDG3457 and MIDG3459), we previously identified single genomic regions with homology to Mu-like bacteriophage and presented preliminary evidence of active phage. Here, updated Phastest genomic analysis identified two loci in both MIDG3457 and MIDG3459 that were predicted to encode proteins with high homology to, and whose organisation was characteristic of, Mu-like phages. Phylogenetically, the closest matches were with Mannheimia Vb and Glaesserella SuMu phages. Phastest scored the loci as "complete", indicating they produced active phage. PCR amplification of the Mu-like phage c and tail genes from DNase-treated polyethylene glycol 8000 (PEG)-precipitated supernatants of MIDG3457 and MIDG3459 (grown in either Brain Heart Infusion-NAD or Grace's Insect Medium-NAD broth) indicated the presence of intact virions. The phages from MIDG3457 and MIDG3459 were named PluMu 3457-1, 3457-2, and PluMu 3459-1 and PluMu 3459-2, respectively. Transmission electron microscopy (TEM) of the PEG-precipitated supernatants of broth-grown MIDG3459 identified virions with icosahedral heads and tails, consistent with other Mu-like phages. We conclude that MIDG3459 produces an active Mu-like phage. [ABSTRACT FROM AUTHOR]