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Nectar analysis has been used to understand pollination systems, but nectar storage methods have rarely been considered as potential sources of inaccuracy in the recovery of data. Prompt nectar sugar analysis is not always possible and storage methods can affect results. We aimed to develop an effective method to store nectar on filter paper. Nectars from two subspecies of Eremophila maculata (Scrophulariaceae) and Strelitzia reginae (Strelitziaceae) were spotted on filter papers. Nectars were redissolved and assayed by high-performance liquid chromatography to determine the masses of sugars recovered from the papers from Day 0 to Day 30. We evaluated the effects of the method of elution, paper type and size, and storage treatments on sugar recovery. Liquid nectars were also stored in the refrigerator. Sugars were best eluted from filter papers in 15 mL of water and agitated for 1 min. Nectar sugars stored on small papers tended to be recovered more successfully than those stored on larger papers (significantly for glucose). Paper performed better than nylon for glucose. Desiccant had a marginal positive effect on nectar sugar recovery, and filter paper performed better than did refrigeration of liquid nectar for storage. If highly accurate measurements are needed, nectars should be eluted with large volumes of water from small filter papers stored with desiccant within a few days of collection. Floral nectar is a primary driver of pollination. Storage of nectar between collection and analysis can greatly affect sugar characteristics. We aimed to develop an effective method to store nectar on filter paper. Nectar is best eluted with large volumes of water from small filter papers stored with desiccant. Nectar storage methods have rarely been considered as sources of inaccuracy; however, we have demonstrated that prompt analysis is necessary for the most reliable results. [ABSTRACT FROM AUTHOR]

Objective: The medical record is critical for documentation and communication between healthcare professionals. This study compared the completeness of orthopaedic documentation between the electronic medical record (EMR) and paper medical record (PMR). Methods: A review was undertaken of 400 medical records (200 EMR, 200 PMR) of patients with operatively managed traumatic lower limb injury. The operative report, discharge summary and first and second out-patient reviews were evaluated using criteria designed by a senior orthopaedic surgeon and senior physiotherapist. The criteria included information deemed critical to the post-operative care of the patient in the first 6 weeks post-surgery. Results: In all cases, an operative report was completed by a senior surgeon. Notable findings included inferior documentation of patient weight-bearing status on the operative report in the EMR than PMR group (P = 0.018). There was a significant improvement in the completion of discharge summaries in the EMR compared with PMR cohort (100% vs 82.5% respectively; P < 0.001). In the PMR group, 70.0% of discharge summaries were completed and adequately documented, compared with 91.5% of those in the EMR group (P < 0.001). At out-patient review, there was an improvement in documentation of weight-bearing instructions in the EMR compared with PMR group (81.1% vs 76.2% respectively; P = 0.032). Conclusion: The EMR is associated with an improvement in the standard of orthopaedic medical record documentation, but deficiencies remain in key components of the medical record. What is known about the topic?: Medical records are an essential tool in modern medical practice and have significant implications for patient care and management, communication and medicolegal issues. Despite the importance of comprehensive documentation, numerous examples of poor documentation continue to be demonstrated. Recently, significant changes to the medical record in Australia have been implemented with the conversion of some hospitals to an EMR and the implementation of the My Health Record. What does this paper add?: Standards of patient care should be monitored continuously and deficiencies identified in order to implement measures for improvement and to close the quality loop. This study has highlighted that although there has been improvement in medical record keeping with the implementation of an EMR, the standard of orthopaedic medical record keeping continues to be below what is expected, and several key areas of documentation require improvement. What are the implications for practitioners?: The implications of these findings for practitioners are to highlight current deficiencies in documentation and promote change in current practice to improve the quality of medical record documentation among medical staff. Although the EMR has improved documentation, there remain areas for further improvement, and hospital administrators will find these observations useful in implementing ongoing change. [ABSTRACT FROM AUTHOR]

Context and aims: Freshly harvested seeds of temperate annual pasture legume species have physiological dormancy (PD), which delays the growth of the next generation and is thus an impediment to speed breeding. The aim of this study was to develop a method or methods of PD breaking that can achieve high germination (≥90%) within 5 days of picking freshly harvested seed. Methods: In a series of experiments, PD breaking methods by themselves and in combination were tested on burr medic (Medicago polymorpha). The most promising methods were then tested on other annual medic species (Medicago spp.), subterranean clover (Trifolium subterraneum), and alternative species from six genera. Key results: The successful PD breaking method involved drying pods overnight at 45°C, extracting and scarifying seed, placing seed on filter paper in a Petri dish along with non-dormant seed, imbibing seed with 4 μM benzylaminopurine, sealing the Petri dish, and incubating in the dark at 15°C. Highly dormant genotypes required the additional treatment of removing the testa. Conclusions: We have developed an effective way of breaking seed PD in the 11 pasture legumes species from the seven genera tested. The rapid breaking of seed PD will assist speed breeding of annual pasture legumes, making it less laborious and time-bound than currently used methods. Implications: The PD breaking methods are expected to assist basic science studies of annual pasture legume species and may be applicable to other species and genera. Freshly harvested seeds of temperate annual pasture legume species have physiological dormancy (PD), which delays the growth of the next generation and is thus an impediment to speed breeding. We report on the development of PD breaking methods that rapidly overcome PD of freshly harvested seed. The rapid breaking of PD assists the speed breeding of annual pasture legumes by making it less laborious and time-bound than previous methods. [ABSTRACT FROM AUTHOR]