1. Clinical Significance of miR-149 in the Survival of Patients with Laryngeal Squamous Cell Carcinoma.
- Author
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Xu, Yi, Lin, Yun-Peng, Yang, Dong, Zhang, Geng, and Zhou, Hui-Fang
- Subjects
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SQUAMOUS cell carcinoma , *RNA analysis , *APOPTOSIS , *CELL culture , *CELL lines , *CELL physiology , *FLOW cytometry , *GENETIC techniques , *POLYMERASE chain reaction , *PROBABILITY theory , *RNA , *T-test (Statistics) , *PROPORTIONAL hazards models , *REVERSE transcriptase polymerase chain reaction , *DATA analysis software , *DESCRIPTIVE statistics , *KAPLAN-Meier estimator , *IN vitro studies , *LOG-rank test , *MANN Whitney U Test , *PROGNOSIS ,LARYNGEAL tumors - Abstract
MicroRNAs (miRNAs) play critical roles in the progression of laryngeal cancer (LC). In this study, we aimed to investigate whether miR-149 is associated with the prognosis of patients with LC. A total of 97 laryngeal squamous cell carcinoma patients who underwent tumor resection were included in our follow-up study. In vitro studies was performed in cancer cell line Hep-2 to explore the antitumor role of miR-149 in LC. We found that the expression of miR-149 was significantly lower in tumor tissues, compared with vocal cord polyp tissues (P<0.05). Kaplan-Meier analysis revealed that miR-149 expression status is significantly associated with survival duration (log rank test, P<0.05), and multivariate Cox regression analysis revealed that patients with low miR-149 expression had shorter survival times compared with patients with high miR-149 expression. In vitro studies revealed that the exogenous expression of miRNA-149 inhibits the proliferation of human Hep-2 cells and induces cell apoptosis. Our study suggests that miR-149 expression in laryngeal squamous cell carcinoma tissues is critically associated with the prognosis of patients, and the ectopic expression of miR-149 in Hep-2 cells inhibits proliferation and cell cycle progression. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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