1. Stem Cell-Like Properties of CK2p-down Regulated Mammary Cells.
- Author
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Duchemin-Pelletier, Eve, Baulard, Megghane, Spreux, Elodie, Prioux, Magali, Burute, Mithila, Mograbi, Baharia, Guyon, Laurent, Théry, Manuel, Cochet, Claude, and Filhol, Odile
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BREAST tumors , *CELL physiology , *CREATINE kinase , *EPITHELIAL cells , *ISOENZYMES , *METASTASIS , *PROTEIN kinases , *STEM cells , *T cells , *GENE expression profiling - Abstract
The ubiquitous protein kinase CK2 has been demonstrated to be overexpressed in a number of human tumours. This enzyme is composed of two catalytic α or α' subunits and a dimer of β regulatory subunits whose expression levels are probably implicated in CK2 regulation. Several recent papers reported that unbalanced expression of CK2 subunits is sufficient to drive epithelial to mesenchymal transition, a process involved in cancer invasion and metastasis. Herein, through transcriptomic and miRNA analysis together with comparison of cellular properties between wild type and CKβ-knock-down MCF10A cells, we show that down-regulation of CKβ subunit in mammary epithelial cells induces the acquisition of stem cell-like properties associated with perturbed polarity, CD44high/CD24low antigenic phenotype and the ability to grow under anchorage-independent conditions. These data demonstrate that a CK2β level establishes a critical cell fate threshold in the control of epithelial cell plasticity. Thus, this regulatory subunit functions as a nodal protein to maintain an epithelial phenotype and its depletion drives breast cell stemness. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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