1. A Nitrocellulose Paper-Based Multi-Well Plate for Point-of-Care ELISA.
- Author
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Qin, Zhen, Huang, Zongjie, Pan, Peng, Pan, Yueyue, Zuo, Runze, Sun, Yu, and Liu, Xinyu
- Subjects
NITROCELLULOSE ,POINT-of-care testing ,OPTICAL scanners ,COVID-19 pandemic ,DETECTION limit ,IMMUNOASSAY - Abstract
Low-cost diagnostic tools for point-of-care immunoassays, such as the paper-based enzyme-linked immunoassay (ELISA), have become increasingly important, especially so in the recent COVID-19 pandemic. ELISA is the gold-standard antibody/antigen sensing method. This paper reports an easy-to-fabricate nitrocellulose (NC) paper plate, coupled with a desktop scanner for ELISA, which provides a higher protein immobilization efficiency than the conventional cellulose paper-based ELISA platforms. The experiments were performed using spiked samples for the direct ELISA of rabbit IgG with a limit of detection (LOD) of 1.016 μg/mL, in a measurement range of 10 ng/mL to 1 mg/mL, and for the sandwich ELISA of sperm protein (SP-10) with an LOD of 88.8 ng/mL, in a measurement range of 1 ng/mL to 100 μg/mL. The described fabrication method, based on laser-cutting, is a highly flexible one-step laser micromachining process, which enables the rapid production of low-cost NC paper-based multi-well plates with different sizes for the ELISA measurements. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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