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Cinnamomum camphora fruit extract was used to biosynthesise silver nanoparticles (AgNPs), and the optimised synthesis system was ascertained through solution colour change and ultraviolet–visible absorption spectra. It contained 20 ml of fruit extract, 4 mM Ag nitrate, and pH 7. AgNPs obtained based on such conditions were spherical and finely dispersed, with an average size of 20.3 nm. As‐synthesised AgNPs exhibited excellent antifungal effect against Fusarium oxysporum. At a dose of 400 μg/ml of AgNPs, the inhibition rate of colony growth reached 61.00% and an IC50 value of 154.39 μg/ml. In addition, the conidia germination was totally inhibited at 100 μg/ml of AgNPs. Results of this study provide a new approach for biological control of plant pathogenic fungi, and it makes that possible for developing a brand new fungistat. [ABSTRACT FROM AUTHOR]