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Noncoding RNAs have been found to play important roles in DNA damage repair, whereas the participation of circRNA remains undisclosed. Here, we characterized ciRS-7, a circRNA containing over 70 putative miR-7-binding sites, as an enhancer of miRISC condensation and DNA repair. Both in vivo and in vitro experiments confirmed the condensation of TNRC6B and AGO2, two core protein components of human miRISC. Moreover, overexpressing ciRS-7 largely increased the condensate number of TNRC6B and AGO2 in cells, while silencing ciRS-7 reduced it. Additionally, miR-7 overexpression also promoted miRISC condensation. Consistent with the previous report that AGO2 participated in RAD51-mediated DNA damage repair, the overexpression of ciRS-7 significantly promoted irradiation-induced DNA damage repair by enhancing RAD51 recruitment. Our results uncover a new role of circRNA in liquid-liquid phase separation and provide new insight into the regulatory mechanism of ciRS-7 on miRISC function and DNA repair. [ABSTRACT FROM AUTHOR]

A lot of poisonous and harmful smoke flow with high temperature will be produced when there is a fire in coal mine underground. The smoke flow will move along mine roadway and have serious danger for underground personnel and devices. So the paper researched movement rule of fire smoke flow of inclined roadway and horizontal roadway: when fire happens in upstream ventilating roadway, fire smoke flow will move downstream of fire area because of functions of fire pressure and buoyancy effect; when fire happens in downstream ventilating roadway, fire pressure is smaller at early stage of fire, so fire smoke flow will move downward along direction of air flow of roadway; with increasing of fire, fire pressure increases constantly, fire smoke flow will move along direction of fresh air flow when air speed of upstream is lower than critical air speed; function of throttling effect is not obvious at early stage of fire in horizontal roadway, fire smoke flow will move along direction of fresh air flow; with increasing of fire, function of throttling effect increases, fire smoke flow will make reverse movement when value of throttling effect is as big as value of ventilating resistance of roadway. The paper has certain guilding function for relief work of fire of roadway.

Because change of mine gas concentration is influenced by various factors, so prediction of mine gas emission ca't get some variables. To solve the problem, the paper proposed a prediction method of mine gas emission based on time series. It introduced implementation of using AR model of time series to predict mine gas emission in details. The experiment result showed that error rate of prediction for mine gas emission with the method was 4.3% and the prediction is reliable.

In order to predict development status of future safety situation of China's coal industry, a prediction model of simple linear regression for annual death numbers and annual coal production in China's coal industry was established, and the model was used to predict annual death numbers and annual coal production of China's coal industry in three years. The prediction result showed that the model has certain feasibility, which can provide reliable theoretical basis for predicting safety situation of China's coal industry.

This paper proposes a novel method for 2D-to-3D video conversion, based on boundary information to automatically generate the depth map. First, we use the Gaussian model to detect foreground objects and then separate the foreground and background. Second, we employ the superpixel algorithm to find the edge information. According to the superpixels, we will assign corresponding hierarchical depth value to initial depth map. From the result of depth value assignment, we detect the edges by Sobel edge detection with two thresholds to strengthen edge information. To identify the boundary pixels, we use a thinning algorithm to modify edge detection. Following these results, we assign the depth value of foreground to refine it. We use four kinds of scanning path for the entire image to create a more accurate depth map. After that, we have the final depth map. Finally, we utilize depth image-based rendering (DIBR) to synthesize left and right view images. After combining the depth map and the original 2D video, a vivid 3D video is produced. [ABSTRACT FROM AUTHOR]

Background: Nimotuzumab is a humanized IgG1 monoclonal antibody specifically targeting EGFR. In this study, we aimed to investigate the molecular mechanisms of nimotuzumab in its effects of enhancing cancer cell radiosensitivity. Principal Finding: Lung cancer A549 cells and breast cancer MCF-7 cells were pretreated with or without nimotuzumab for 24 h before radiation to perform the clonogenic survival assay and to analyze the cell apoptosis by flow ctyometry. γ-H2AX foci were detected by confocal microscopy to assess the effect of nimotuzumab on radiation induced DNA repair. EGFR activation was examined and the levels of DNA damage repair related proteins in A549 cells at different time point and at varying doses exposure after nimotuzumab and radiation treatment were examined by Western blot. Pretreatment with nimotuzumab reduced clonogenic survival after radiation, inhibited radiation-induced EGFR activation and increased the radiation-induced apoptosis in both A549 cells and MCF-7 cells. The foci of γ-H2AX 24 h after radiation significantly increased in nimotuzumab pretreated cells with different doses. The phosphorylation of AKT and DNA-PKcs were remarkably inhibited in the combination group at each dose point as well as time point. Conclusions: Our results revealed that the possible mechanism of nimotuzumab enhancing the cancer radiosensitivity is that nimotuzumab inhibited the radiation-induced activation of DNA-PKcs through blocking the PI3K/AKT pathway, which ultimately affected the DNA DSBs repair. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the effectiveness and safety of intragastric balloon (IGB) for the treatment of obese Chinese., Methods: IGB was placed into the stomachs of 48 Chinese patients in Hong Kong, 13 males and 35 females; aged 39 +/- 9 (18 - 65), with the mean baseline body weight (BW) of 106 +/- 26 kg and mean body mass index (BMI) of (40 +/- 9) kg/m(2), 34 (70.8%) with coexistent obesity-related morbidities, who failed to respond to other weight reducing treatment, via routine gastroscopy under intravenous conscious sedation. Restricted balanced diet with 1200 kcal/day and exercise of 150 minutes/week were prescribed after the balloon placement. The IGB was removed endoscopically after at most 180 days. Follow-up was conducted once a week during the first month and then once a month., Results: The median hospital stay and treatment duration were 1 (IQR 1:2.25) day and 174 (IQR 166:181) days respectively. The BW, BMI, and waist circumference were significantly decreased after the IGB placement (all P < 0.01) with the mean BW loss of (13 +/- 7) kg, mean BMI loss of (5 +/- 3) kg/m(2), mean excessive body weight loss of (45 +/- 36)%, and mean waist circumference loss of (12 +/- 8) cm. 66.7% of the patients were satisfied with the treatment. No serious complication related to IGB was noted., Conclusion: IGB is a safe and effective device that achieves moderate weight loss in obese Chinese.

Aim: To analyze the clinical and dosimetric predictive factors for radiation-induced esophageal injury in patients with non-small-cell lung cancer (NSCLC) during three-dimensional conformal radiotherapy (3D-CRT)., Methods: We retrospectively analyzed 208 consecutive patients (146 men and 62 women) with NSCLC treated with 3D-CRT. The median age of the patients was 64 years (range 35-87 years). The clinical and treatment parameters including gender, age, performance status, sequential chemotherapy, concurrent chemotherapy, presence of carinal or subcarinal lymph nodes, pretreatment weight loss, mean dose to the entire esophagus, maximal point dose to the esophagus, and percentage of volume of esophagus receiving >55 Gy were studied. Clinical and dosimetric factors for radiation-induced acute and late grade 3-5 esophageal injury were analyzed according to Radiation Therapy Oncology Group (RTOG) criteria., Results: Twenty-five (12%) of the two hundred and eight patients developed acute or late grade 3-5 esophageal injury. Among them, nine patients had both acute and late grade 3-5 esophageal injury, two died of late esophageal perforation. Concurrent chemotherapy and maximal point dose to the esophagus > or =60 Gy were significantly associated with the risk of grade 3-5 esophageal injury. Fifty-four (26%) of the two hundred and eight patients received concurrent chemotherapy. Among them, 25 (46%) developed grade 3-5 esophageal injury (P = 0.0001<0.01). However, no grade 3-5 esophageal injury occurred in patients who received a maximal point dose to the esophagus <60 Gy (P = 0.0001<0.01)., Conclusion: Concurrent chemotherapy and the maximal esophageal point dose > or =60 Gy are significantly associated with the risk of grade 3-5 esophageal injury in patients with NSCLC treated with 3D-CRT.