Search

[Display omitted] • Ninhydrin and Isatin-based chromogenic chemosensors selective for Cr3+ and Pb2+ ions were established. • NMR, FTIR, UV–vis and ESI-MS spectroscopy methods were used to confirm the probe and interaction of the probe with the analytes. • Probe L1 and L2 effective sense at colorimetric method was observed to be 2.80 × 10-6 M and 1.63 × 10-6 M detection limits. • Test Strip was designed to monitor the absorbance of Cr3+ and Pb2+ ions in real time analysis. • In a molecular logic circuit, the reversible and reproducible colorimetric switching off L1 and L2 was also discovered. A simple and easily synthesizable chromogenic small molecules probe L4 (2-(2-(benzo[ d ]thiazol-2-yl)hydrazono)-1H-indene-1,3(2H)-dione) and L5 (2-(2-(benzo[ d ]thiazol-2-yl)hydrazono)indolin-3-one) have been synthesized and characterized by various spectroscopy techniques such as FT-IR, NMR, and HRMS. The colorimetric naked eye detection of probe L4 showed a color change towards Cr3+ from orange color to pale yellow, whereas in case of L5 Pb2+ yellow to orange color was witnessed. The jobs plot analyses indicate the 1:1 binding stoichiometric for Cr3+ and 1:2 binding stoichiometric for Pb2+ ions with probes L4 and L5 respectively. The binding association constant (k a) values for L4 and L5 were found to be 6.651 × 10-5 M for Cr3+ and 6.493 × 10-5 M for Pb2+ ions. The limit of detection was found to be for Cr3+ (1.69 × 10−7 M) and Pb2+ (1.208 × 10−7 M) are far below than the limit set by the World Health Organization (WHO) for drinking water. Finally, the designed probes were applied for visible colorimetric test kits and real samples for rapid detection of Cr3+ and Pb2+ for all practical purposes, indicating their potential use in environmental samples. [ABSTRACT FROM AUTHOR]

Background: Macrophages play a serious part in the instigation, upkeep, and resolution of inflammation. They are activated or deactivated during inflammation progression. Activation signals include cytokines (IF-γ, granulocyte-monocyte colonystimulating factor (GM-CSF), and TNF-α), extracellular matrix proteins, and other chemical mediators. Activated macrophages are deactivated by anti-inflammatory cytokines (IL- 10 and TGF-β (transforming growth factor-beta) and cytokine antagonists that are mainly produced by macrophages. Based on this, the present study aimed to develop novel (E)- Benzylidene-indazolpyridin methanones (Cpd-1-10) as effective anti-inflammatory agents by analyzing pro- and anti-inflammatory cytokine levels in macrophages., Objectives: To determine the anti-inflammatory effect of indazolpyridin-methanones by examining pro- and anti-inflammatory interleukin levels in J77A.1 macrophages., Methods: Expression of cytokines such as TNF-α, IL-1β, IL-6 and IL-10 serum levels measured by ELISA method. Anti-cancer and cytotoxicity studies were carried out by MTT assay. COX-2 seems to be associated with cancers and atypical developments in the duodenal tract. So, a competitive ELISA based COX-2 inhibition assay was done. To validate the inhibitory potentials and to get more insight into the interaction of COX-2 with Cpd1-10, molecular docking was performed., Results: Briefly, the COX-2 inhibitory relative activity was found to be in between the range of 80-92% (Diclofenac showed 84%, IC50 0.95 μM)., Conclusion: Cytotoxicity effect of the compounds against breast cancer cell lines found excellent and an extended anticancer study ensured that these compounds are also alternative therapeutic agents against breast cancer. Among all the tested cancer cell lines, the anti- cancer effect on breast cancer was exceptional for the most active compounds Cpd5 and Cpd9., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)