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Objective To investigate the effect of different maintenance doses of caffeine citrate treatment on early-stage pulmonary function and complications in preterm infants. Methods A total of 78 preterm infants with a gestational age of 28 weeks to 33+6 weeks who were admitted to our hospital from April 2019 to January 2022 and did not need invasive mechanical ventilation after birth were enrolled as subjects, and they were divided into high-maintenance-dose caffeine citrate group with 40 infants and low-maintenance-dose caffeine citrate group with 38 infants using a random number table. Both groups were given peripheral intravenous pumping of caffeine citrate at a loading dose of 20 mg/(kg·d) at 2 h after birth, and then the high and low maintenance dose groups were given caffeine citrate at a maintenance dose of 10 mg/(kg·d) and 5 mg/(kg·d), respectively, after 24 h. The two groups were compared in terms of pulmonary function, complications (necrotizing enterocolitis, retinopathy of prematurity, and intraventricular hemorrhage), and adverse reactions (feeding intolerance, hyperglycemia, and tachycardia) on days 7 and 14 after birth and at the corrected gestational age of 40 weeks. Results Compared with the low maintenance dose group, the high maintenance dose group had significantly higher tidal volume, ratio of time to peak tidal expiratory flow to total expiratory time, and ratio of volume to peak tidal expiratory flow to total expiratory volume on days 7 and 14 after birth and at the corrected gestational age of 40 weeks, as well as a significantly higher respiratory rate on days 7 and 14 after birth (t=2.107-4.128,P0.05). Conclusion A high maintenance dose of caffeine citrate can significantly improve early-stage pulmonary function in preterm infants without increasing related complications and adverse reactions.

Chloride attack severely impacts the performance of reinforced concrete. The total and free chloride ion concentration (CIC) of self-compacting concrete (SCC) prepared with three supplementary cementitious materials (SCM) – fly ash (FA), blast furnace slag (BS) and silica fume (SF) – were measured by accelerated salt immersion tests. The apparent chloride diffusion coefficient (CDC) at any exposure time and erosion depth were calculated using the Boltzmann–Matano method. The influence of the type and content of SCM, the water–binder ratio (W/B) and the type of salt solution on CIC and CDC were investigated. Both the introduction of SCM and the reduction of W/B effectively reduced the CIC. The SCM that most effectively reduced CIC was SF, followed by BS and then FA. Free CIC was reduced to a greater degree than total CIC in FA and BS concrete, but the opposite was true for SF concrete. The presence of calcium chloride in salt solution increased total CIC while reducing free CIC. Apparent free CDC dropped over exposure time and initially increased with erosion depth but eventually stabilised. A model of apparent free CDC considering time–depth dependency was created, which shows that time reduction factors of CDC is greater in SCC containing SCM than in control SCC. [ABSTRACT FROM AUTHOR]

For high-speed railway sound barriers, determining the aerodynamic pressure generated by high-speed trains is crucial for their structural design. This paper investigates the distribution of aerodynamic pressure on the sound barrier caused by high-speed trains with different nose lengths, utilizing the computational fluid dynamics (CFD) simulation method. The accuracy of the numerical simulation method employed is verified through comparison with field test results from the literature. Research findings reveal that when a high-speed train passes through a sound barrier, significant "head wave" and "wake wave" effects occur, with the pressure peak of the "head wave" being notably greater than that of the "wake wave". As the distance between the sound barrier and the center of the train gradually increases, the aerodynamic pressure on the sound barrier gradually decreases. The nose length of the train has a considerable impact on the aerodynamic pressure exerted on the sound barrier. The streamlined shape of longer-nose trains can significantly reduce the aerodynamic effects on the sound barrier, resulting in a notably smaller pressure peak compared to shorter-nose trains. Finally, by establishing the relationship between the train nose length and the aerodynamic pressure peak, a calculation formula for the train-induced aerodynamic pressure acting on the sound barrier is proposed, taking into account the nose length of the high-speed train. [ABSTRACT FROM AUTHOR]

Introduction: Asthma has been attributed to Th1/Th2 imbalance and inappropriate Th2 responses to environmental allergens. MicroRNAs (miRNAs), 21 to 23 RNA molecules, are first found in mammals and have been implicated in various biological activities. Our previous study found that miR-410 effectively ameliorates airway inflammation in the ovalbumin (OVA)-induced asthma murine model. However, the role of miR-410 in regulating helper T (Th) cell differentiation is not clear. In the present study, we aimed to explore the regulatory effects of miR-410 on the differentiation of Th cells through both in vivo and in vitro studies. Methods: Dual-luciferase reporter assay was used to find if miR-410 has any direct binding position with VEGF mRNAs. PBMC and CD4+ T cells were isolated and stimulated with OVA. The miR-410 mimics and inhibitors were transfected into CD4+ T cells. The differentiation of Th cells was evaluated by enzyme-linked immunosorbent assay (ELISA) for the concentration of IL-4, IFN-γ, and TGF-β levels in supernatants. Western Blot was used to detect protein expression and phosphorylation of PI3K and AKT. BALB/c mice were kept in a specific pathogen-free condition and received sterile OVA-free food and water. OVA-induced asthmatic mice model was established. ELISA was used to measure the bronchoalveolar lavage fluid (BALF) concentrations of IL-4, IFN-γ, TGF-β, and VEGF. Hematoxylin and eosin staining and immunohistochemical staining were conducted to analyze inflammatory cell infiltration, pathological changes, and the expression of VEGF. Results: Dual-luciferase reporter assay showed that miR-410 has no direct binding position with VEGF mRNAs. In the OVA-primed mononuclear cells compared to normal cells, IFN-γ and TGF-β were decreased while IL-4 and VEGF were increased. This change was reversed while miRNA-410 mimics were transfected into CD4+ T cells. Besides, the OVA-primed CD4+ T cells treated with miR-410 decrease the proliferation of cytokine of Th2 cells as well as phosphorylation of PI3K, and AKT. In OVA-induced asthma mice, IFN-γ and TGF-β were decreased in BALF while the IL-4 and VEGF were increased. OVA-induced mice with asthma treated with miR-410 mimics showed marked reductions in the infiltration of inflammatory cells as well as IL-4 and VEGF in BALF. The immunohistochemical staining of the expression of VEGF also decreased in OVA-induced asthma mice with the instillation of miR-410. Conclusions: In this study, we revealed that miR-410 could regulate the differentiation of Th cells via the PI3K-AKT-VEGF signaling pathway in asthma. [ABSTRACT FROM AUTHOR]

Land cover changes (LCCs) due to urbanization cause urban heat islands (UHIs), significantly affecting land surface temperature (LST) through spatiotemporal changes in compositions, parameters, and patterns. Land cover and LST have been studied in various cities; however, indicative research into heterogeneous LCC's impact on LST in less-developed cities remains incomplete. This study analyzed new Landsat images of Zhanjiang, taken from 2004 to 2022, to determine the impact of three LCC indicators (compositions, parameters, and patterns) on LSTs. The urban thermal field variance index (UTFVI) was used to describe the distribution and variation in LST. We also quantified the cooling or warming benefits of various LCCs. The results indicate that the average temperature in the land urban heat island (SUHI) area rose to 30.6 °C. The average temperature of the SUHI was 3.32 °C higher than that of the non-SUHI area, showing the characteristic of shifting to counties and multi-core development. The LST increases by 0.37–0.67 °C with an increase of 0.1 in the normalized difference building index (NDBI), which is greater than the cooling benefit of the normalized difference of vegetation index (NDVI). The impact of landscape pattern indices on impervious surfaces and water is higher than that on vegetation and cropland, with a rising influence on impervious surfaces and a decreasing impact on water. The predominant cooling patches are vegetation and water, while large areas of impervious surface and cropland aggravate UHIs for industrial and agricultural activities. These findings are intended to guide future urban layouts and planning in less-developed cities, with thermal climate mitigation as a guiding principle. [ABSTRACT FROM AUTHOR]

Rapid urbanization is changing the existing patterns of land use land cover (LULC) globally, which is consequently increasing the land surface temperature (LST) in many regions. The present study is focused on estimating current and simulating future LULC and LST trends in the urban environment of Chaoyang District, Beijing. Past patterns of LULC and LST were identified through the maximum likelihood classification (MLC) method and multispectral Landsat satellite images during the 1990–2018 data period. The cellular automata (CA) and stochastic transition matrix of the Markov model were applied to simulate future (2025) LULC and LST changes, respectively, using their past patterns. The CA model was validated for the simulated and estimated LULC for 1990–2018, with an overall Kappa (K) value of 0.83, using validation modules in IDRISI software. Our results indicated that the cumulative changes in built-up to vegetation area were 74.61 km2 (16.08%) and 113.13 km2 (24.38%) from 1990 to 2018. The correlation coefficient of land use and land cover change (LULCC), including vegetation, water bodies and built-up area, had values of r = − 0.155 (p > 0.005), −0.809 (p = 0.000), and 0.519 (p > 0.005), respectively. The results of future analysis revealed that there will be an estimated 164.92 km2 (−12%) decrease in vegetation area, while an expansion of approximately 283.04 km2 (6% change) will occur in built-up areas from 1990 to 2025. This decrease in vegetation cover and expansion of settlements would likely cause a rise of approximately ∼10.74 °C and ∼12.66 °C in future temperature, which would cause a rise in temperature (2025). The analyses could open an avenue regarding how to manage urban land cover patterns to enhance the resilience of cities to climate warming. This study provides scientific insights for environmental development and sustainability through efficient and effective urban planning and management in Beijing and will also help strengthen other research related to the UHI phenomenon in other parts of the world.

In this study, the whole chloroplast genome of Ulmus glabra ‘Pendula’ was sequenced, assembled, annotated and characterized for the first time. The results showed that the total length of the chloroplast genome of U. glabra ‘Pendula’ was 159,305 bp and the GC content was 35.58%. It had a circular tetrad structure of the chloroplast genome of typical angiosperms, including a large single-copy (LSC) with length of 87,916 bp, a small single-copy (SSC) with length of 18,693 bp, and a pair of inverted repeat sequence (IRs) of 26,348 bp. A total of 131 genes were identified, including 86 protein coding genes, 37 tRNA genes, and 8 rRNA genes. Based on the whole chloroplast genome sequence, the phylogenetic trees of 14 plants in U. glabra ‘Pendula’ and NCBI database were constructed, and the phylogenetic position of U. glabra ‘Pendula’ in Ulmaceae was identified. The results provided important information for the identification of genetic relationship of U. glabra ‘Pendula’, and will also promote the functional analysis of genes related to agronomic traits controlled by the chloroplast genome of U. glabra ‘Pendula’.

Landfill leachate has a high chloride (Cl−) content. Because it is highly mobile, and cannot be sorbed or transformed bio-chemically, it is important to have detailed information about how it migrates in landfill sites. In this study, we set up four lab-scale simulated landfills, including an anaerobic landfill (AL), an anaerobic landfill with leachate recirculation (RAL), an anaerobic/semi-anaerobic landfill with leachate recirculation (RASL), and an anaerobic/semi-aerobic landfill (ASL), to explore how, when regulated, moisture and air affected the migration of chlorine. We found that water and air had a strong influence on the release of Cl−. Leachate obviously promoted Cl− dissolution in refuse when recirculated. When air was introduced into landfill, thereby changing it from anaerobic to semi-aerobic, the leachate Cl− concentration increased sharply from around 4–9 g L−1 (RASL) and 18 g L−1 (ASL), respectively. In principle, Cl− is released continuously when leachate is recirculated in landfills (RAL and RASL), but it can also be found a terminal when the leachate recirculation stops (AL and ASL). Cumulative amounts of 64, 66, 27, and 53 g of Cl− were released from the AL, RAL, RASL, and ASL, respectively. Lower COD/Cl and N H 4 + -N/Cl ratios in ASL and RASL after day 175 indicated that lower Cl− pollution risk than that in AL and RAL. [ABSTRACT FROM AUTHOR]

Obesity is increasingly prevalent globally, searching for therapeutic agents acting on adipose tissue is of great importance. Equisetin (EQST), a meroterpenoid isolated from a marine sponge-derived fungus, has been reported to display antibacterial and antiviral activities. Here, we revealed that EQST displayed anti-obesity effects acting on adipose tissue through inhibiting adipogenesis in vitro and attenuating HFD-induced obesity in mice, doing so without affecting food intake, blood pressure or heart rate. We demonstrated that EQST inhibited the enzyme activity of 11 β -hydroxysteroid dehydrogenase type 1 (11 β -HSD1), a therapeutic target of obesity in adipose tissue. Anti-obesity properties of EQST were all offset by applying excessive 11 β -HSD1's substrates and 11 β -HSD1 inhibition through knockdown in vitro or 11 β -HSD1 knockout in vivo. In the 11 β -HSD1 bypass model constructed by adding excess 11 β -HSD1 products, EQST's anti-obesity effects disappeared. Furthermore, EQST directly bond to 11 β -HSD1 protein and presented remarkable better intensity on 11 β -HSD1 inhibition and better efficacy on anti-obesity than known 11 β -HSD1 inhibitor. Therefore, EQST can be developed into anti-obesity candidate compound, and this study may provide more clues for developing higher effective 11 β -HSD1 inhibitors. Equisetin suppresses lipid accumulation in adipose tissue to curb diet-induced obesity through inhibiting 11 β -HSD1 activity. [Display omitted] [ABSTRACT FROM AUTHOR]

Meckel syndrome (MKS), also known as the Meckel–Gruber syndrome, is a severe pleiotropic autosomal recessive developmental disorder caused by dysfunction of the primary cilia during early embryogenesis. The diagnostic criteria are based on clinical variability and genetic heterogeneity. Mutations in the MKS1 gene constitute approximately 7% of all MKS cases. Herein, we present a non-consanguineous couple with three abnormal pregnancies as the fetuses showed MKS-related phenotypes of the central nervous system malformation and postaxial polydactyly. Whole-exome sequencing identified two novel heterozygous mutations of MKS1 : c.350C>A and c.1408-14A>G. The nonsense mutation c.350C>A produced a premature stop codon and induced the truncation of the MKS1 protein (p.S117*). Reverse-transcription polymerase chain reaction (RT-PCR) showed that c.1408-14A>G skipped exon 16 and encoded the mutant MKS1 p.E471Lfs*92. Functional studies showed that these two mutations disrupted the B9–C2 domain of the MKS1 protein and attenuated the interactions with B9D2, the essential component of the ciliary transition zone. The couple finally got a healthy baby through preimplantation genetic testing for monogenic disorder (PGT-M) with haplotype linkage analysis. Thus, this study expanded the mutation spectrum of MKS1 and elucidated the genetic heterogeneity of MKS1 in clinical cases. [ABSTRACT FROM AUTHOR]

The corrosion resistance of concrete strengthened with three types of fibre-reinforced polymer (FRP) sheets (unidirectional carbon-FRP (CFRP), unidirectional basalt-FRP (BFRP) and bidirectional BFRP) was evaluated under chloride attack and the combined attack of chloride and ultraviolet radiation. After different corrosion durations, powder samples were collected from various depths of the concrete specimens to measure the total chloride ion concentrations (CICs). Scanning electron microscopy (SEM) was conducted to analyse the microscale morphology changes of the concrete surface layer, FRP fibre layer and resin matrix layer before and after combined attack. The results showed that all three types of FRP sheet provided significant chloride ion resistance, with the barrier effect of CFRP being better than that of BFRP under the same conditions. The total CIC of the FRP-strengthened concretes was higher under the combined attack than under chloride attack alone. The SEM observations showed that the resin matrix and fibre filaments of the FRP layer under combined attack were damaged, resulting in an increase in the permeability of the FRP-strengthened concretes. Finally, a time-varying model of CIC inside the FRP-strengthened concrete under chloride attack was established. [ABSTRACT FROM AUTHOR]

Calcium silicate hydrate (C-S-H) is the main hydration product of cementitious materials, often experiencing complex stress conditions in practical applications. Therefore, reactive molecular dynamics methods were used to investigate the mechanical response of the atomistic structure of C-S-H under various uniaxial and biaxial strain conditions. The results of uniaxial simulations show that C-S-H exhibits mechanical anisotropy and tension–compression asymmetry due to its layered atomistic structure. By fitting the stress–strain data, a stress–strain relationship that accurately represents the elastoplasticity of C-S-H was developed. The biaxial yield surface obtained from biaxial simulations was ellipsoidal, again reflecting the anisotropy and asymmetry of C-S-H. Four yield criteria (von Mises, Drucker–Prager, Hill, and Liu–Huang–Stout) were further investigated, and it was found that the Liu–Huang–Stout criterion can effectively capture all the major features of the yield surface. During a uniaxial tensile process in the z direction, multi-crack propagation was observed, which was aggravated and weakened by y direction tensile and compressive strains respectively. The results of chemical bond analyses revealed that, for different strain conditions, the CaW–OS and CaS–OS bonds play different roles in resisting deformation. [ABSTRACT FROM AUTHOR]

Objective: Biopsies are the gold standard for clinical diagnosis. However, a discrepancy between the biopsy sample and target tissue because of misplacement of the biopsy spoon can lead to errors in the diagnosis and subsequent treatment. Thus, correctly determining whether the needle tip is in the tumor is crucial for accurate biopsy results. Methods: A biopsy needle system was designed with a steerable, flexible, and superelastic concentric tube; electrodes to monitor the electrical resistivity; and load cells to monitor the insertion force. The degrees of freedom were analyzed for two working modes: straight-line and deflection. Results: Experimental results showed that the system could perceive the tissue type in online based on the electrical resistivity. In addition, changes in the insertion force indicated transitions between the interfaces of adjacent tissue layers. Conclusion: The two monitoring methods guarantee that the biopsy spoon is at the desired position inside the tumor during an operation. Significance: The proposed biopsy needle system can be integrated into an autonomous robotic biopsy system. [ABSTRACT FROM AUTHOR]

Rapid urbanization is changing the existing patterns of land use land cover (LULC) globally, which is consequently increasing the land surface temperature (LST) in many regions. The present study is focused on estimating current and simulating future LULC and LST trends in the urban environment of Chaoyang District, Beijing. Past patterns of LULC and LST were identified through the maximum likelihood classification (MLC) method and multispectral Landsat satellite images during the 1990–2018 data period. The cellular automata (CA) and stochastic transition matrix of the Markov model were applied to simulate future (2025) LULC and LST changes, respectively, using their past patterns. The CA model was validated for the simulated and estimated LULC for 1990–2018, with an overall Kappa (K) value of 0.83, using validation modules in IDRISI software. Our results indicated that the cumulative changes in built-up to vegetation area were 74.61 km2 (16.08%) and 113.13 km2 (24.38%) from 1990 to 2018. The correlation coefficient of land use and land cover change (LULCC), including vegetation, water bodies and built-up area, had values of r = − 0.155 (p > 0.005), −0.809 (p = 0.000), and 0.519 (p > 0.005), respectively. The results of future analysis revealed that there will be an estimated 164.92 km2 (−12%) decrease in vegetation area, while an expansion of approximately 283.04 km2 (6% change) will occur in built-up areas from 1990 to 2025. This decrease in vegetation cover and expansion of settlements would likely cause a rise of approximately ∼10.74 °C and ∼12.66 °C in future temperature, which would cause a rise in temperature (2025). The analyses could open an avenue regarding how to manage urban land cover patterns to enhance the resilience of cities to climate warming. This study provides scientific insights for environmental development and sustainability through efficient and effective urban planning and management in Beijing and will also help strengthen other research related to the UHI phenomenon in other parts of the world [ABSTRACT FROM AUTHOR]

Based on field investigation and literature review, the techniques of folk embroidery in Shaanxi Province was classified and analyzed. It is concluded that Shaanxi folk embroidery refers to the general term of making plane embroidery into all kinds of crafts, including all kinds of plane and three-dimensional embroidery, and its shape varies widely. Based on the classification of techniques, it can be roughly divided into four categories: soft sculpture, cloth stitching, cloth stitching and cloth embroidery. Each classification of techniques has formed the local style and characteristics, and many kinds of techniques are used, such as collage, stitching, embroidery, twisting, decoration and sculpture. The needling method is rich and varied, and has local characteristics. The study of Shaanxi folk embroidery techniques can promote the inheritance and development of folk intangible cultural heritage, while maintain cultural diversity and promote cultural prosperity. [ABSTRACT FROM AUTHOR]

• Mutual effects of Se and PGPR on Se-enriched biofortification were explored. • The combination of low-dose PGPR and exogenous Se promoted plant growth. • Se content in plant's edible parts significantly increased with rising doses of PGPR and Se. • SeMet, SeMeCys and SeCys2 were the main Se forms in plant's edible parts. • Optimal combinations of PGPR and Se for Se biofortification were obtained by Two-way ANOVA. Using plant growth promoting rhizobacteria (PGPR) to boost Se uptake and accumulation in crops is a promising recent approach to biofortification. Most studies, however, have focused on a certain dose of PGPR or Se, while the synergistic effects of simultaneously changing the concentration of both exogenous Se and PGPR on plant growth and Se bioaccumulation are rarely explored. In this study, the combined treatments of 0-2 mg L−1 Se and (3.83-11.3) ×107 CFU mL−1 PGPR strain Enterobacter sp. EG16 were used for the hydroponic experiments of pak choi (Brassica rapa) to investigate the mutual effects on plant growth and Se absorption. The growth of pak choi, in terms of plant height, number of leaves and root tips, was promoted by combining 7.65×107 CFU mL−1 EG16 and no more than 1 mg L−1 of Se. Growth was significantly inhibited when the concentration of EG16 was raised to 11.3×107 CFU mL−1. Chlorophyll content, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities were also boosted under the same combination of Se and EG16 concentrations. In addition, the Se content of aboveground part and intact plants significantly increased with rising concentrations of EG16 and Se. Selenomethionine (SeMet), methylselenocysteine (SeMeCys) and selenocystine (SeCys2) were the main organo-Se compounds detected in pak choi, accounting for almost 90% when supplied with selenite. Among these compounds, SeMet accounted for a high proportion and SeCys2 for a relatively low percentage in most treatments. The two forms were inversely related, probably because the transformation was driven by EG16. On the other hand, the proportion of SeMeCys was significantly affected by different concentrations of exogenous Se and EG16. Selenate was also detected, indicative of selenite oxidation. In terms of both plant biomass and nutritional quality, the combined formulation of 1 mg L−1 Se and 7.65×107 CFU mL−1 of EG16 is a promising means of producing Se-enriched vegetables. [ABSTRACT FROM AUTHOR]

Nao-Mai-Tong (NMT), a Chinese herbal formula, is used for treating ischemia cerebral apoplexy. To discover the components of NMT with potential in vivo bioactivity and investigate the differences between NMT and its single herb components, this study rigorously compared plasma samples collected from rats dosed with NMT and single-herb extracts at different times through LC-high resolution-MS/MS and data processing (Metworks). The plasma of the NMT group contained a total of 66 identified compounds (25 prototypes and 41 metabolites), including anthraquinones, triterpenoid saponins, isoflavones and phthalides. Additionally, glucuronidation, sulfation and cysteine conjugation were the major reactions through which the compounds in NMT were metabolized. The comparison of the groups revealed two metabolites that were only detected in the plasma from the NMT-dosed group, whereas seven prototype ingredients (chrysophanol-8- O-glucoside, ginsenoside Rf, Rg2, Rh1, F1, F2 and chikusetsusaponin IVa) and 12 metabolites (two novel triterpenoid saponins) were only discovered in the plasma samples from the single-herb-dosed groups. Moreover, the trends in the chemical compounds detected presented marked differences between NMT-dosed rat plasma and plasma samples from the single-herb-dosed groups. The above data indicate that prescription compatibility affects the assimilation and elimination of ingredients and provides useful information for further pharmacokinetic studies. [ABSTRACT FROM AUTHOR]

• Novel variants of ZP1, ZP2 and ZP3 are associated with female infertility. • In-vitro functional studies of ZP protein variants indicate pathogenesis. • The ZP3 variant was overcome with ICSI treatment. • Targeted genetic diagnosis of ZP genes may guide fertilization methods. Which genetic variants might explain the causes of empty follicle syndrome (EFS) and abnormal zona pellucida (ZP) and affect the success of treatment with assisted reproductive technologies (ART)? Whole-exome sequencing was performed in probands with EFS and abnormal ZP. Sanger sequencing was used for variant validation. Using HEK-293T cells, the effects of ZP1 and ZP2 variants on protein expression were explored by western blotting, and the effect of the ZP1 variant on protein location was investigated via immunofluorescence. The protein structure was also analysed to investigate the pathogenicity of variants. A homozygous nonsense variant in ZP1 (c.874C>T, p.Gln292*) was detected in a patient with EFS. A novel homozygous frameshift variant in ZP2 (c.836_837delAG, p.Glu279Valfs*6) and a novel heterozygous missense variant in ZP3 (c.1159G>A, p.Val387Met) were identified in two patients with ZP morphological abnormalities, respectively. Western blotting and immunofluorescence analysis showed that the ZP1 variant results in a premature stop codon, leading to the truncated ZP1 protein. The ZP2 variant, which is situated in the N-terminus, triggers the degradation of a premature termination protein. Additionally, the patient with the ZP3 variant achieved clinical pregnancy following intracytoplasmic sperm injection treatment. These findings expand the mutational spectrum of ZP1, ZP2 and ZP3 , and provide new evidence for genetic diagnosis of female infertility. The targeted genetic diagnosis of ZP genes is recommended to choose appropriate fertilization methods and improve success rates of treatment with ART. [ABSTRACT FROM AUTHOR]

Small interfering RNAs (siRNAs) directed against poliovirus (PV) and other viruses effectively inhibit viral replication and have been developed as antiviral agents. Here, we demonstrate that a specific siRNA targeting the region between nucleotides 100-125 (siRNA-100) from the 5′-untranslated region (5′-UTR) of PV plays a critical role in inhibiting PV replication. Our data demonstrate that siRNA-100 treatment can greatly reduce PV titers, resulting in up-regulation of host microRNA-7 (miR-7), which in turn, leads to enhance inhibition of PV infection further. Moreover, our results suggest that siRNA-100 can also impair the spread of PV to uninfected cells by increasing host resistance to PV, resulting in decreasing necrosis and cytopathic effects (CPE) levels, as well as prolonging the survival of infected cells. Indeed, the active antiviral effect of siRNA-100 was potentially supplemented by the activity of miR-7, and both of them can serve as stabilizing factors for maintenance of cellular homeostasis. Results of this study identify a molecular mechanism of RNAi for antiviral defense, and extend our knowledge of the complex interplay between host and PV, which will provide a basis for the development of effective RNAi-based therapies designed to inhibit PV replication and protect host cells. [ABSTRACT FROM AUTHOR]

Background: Distillers dried grains with solubles (DDGS) are rich in nutrition, and they are potential protein feed raw material. However, the existence of cellulose, hemicellulose and lignin hinders animals' digestion and absorption of DDGS. Making full use of unconventional feed resources such as DDGS can alleviate the shortage of feed resources to a certain extent. This research investigated the effects of twin-screw extrusion on the macromolecular composition, physical and chemical properties, surface structure and in vitro protein digestibility (IVPD) of DDGS., Results: The findings showed that extrusion puffing significantly increased the protein solubility, bulk density, water holding capacity, and swelling capacity, while significantly decreased hemicellulose and crude protein content, particle size and zeta potential of DDGS. The structure damage of DDGS induced by the extrusion was characterized by scanning electron microscopy (SEM), Fourier-transform infrared (FITR) spectroscopy and X-ray diffraction (XRD) analysis. Interestingly, no random coil was observed in the analysis of the secondary structure, and extrusion promoted the transformation of α-helix and β-turn to β-sheet, which led to significant increases in protein solubility and IVPD of DDGS (P < 0.05). Additionally, correlation analysis revealed that IVPD and PS had a positive relationship., Conclusion: Extrusion puffing was an ideal pretreatment method for DDGS modification to improve in vitro protein digestibility. © 2023 Society of Chemical Industry., (© 2023 Society of Chemical Industry.)

Backgrounds and Aims: Bronchopulmonary dysplasia (BPD) has serious immediate and long-term sequelae as well as morbidity and mortality. The objective of this study is to develop a predictive model of BPD for premature infants using clinical maternal and neonatal parameters., Methods: This single-center retrospective study enrolled 237 cases of premature infants with gestational age less than 32 weeks. The research collected demographic, clinical and laboratory parameters. Univariate logistic regression analysis was carried out to screen the potential risk factors of BPD. Multivariate and LASSO logistic regression analysis was performed to further select variables for the establishment of nomogram models. The discrimination of the model was assessed by C-index. The Hosmer-Lemeshow test was used to assess the calibration of the model., Results: Multivariate analysis identified maternal age, delivery option, neonatal weight and age, invasive ventilation, and hemoglobin as risk predictors. LASSO analysis selected delivery option, neonatal weight and age, invasive ventilation, hemoglobin and albumin as the risk predictors. Both multivariate (AUC = 0.9051; HL P  = 0.6920; C-index = 0.910) and LASSO (AUC = 0.8935; HL P  = 0.7796; C-index = 0.899) - based nomograms exhibited ideal discrimination and calibration as confirmed by validation dataset., Conclusions: The probability of BPD in a premature infant could be effectively predicted by the nomogram model based on the clinical maternal and neonatal parameters. However, the model required external validation using larger samples from multiple medical centers., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2023 Gao, Liu, Guo and Cao.)

Background: This study aims to describe clinical and diagnostic phenotype and identify pathogenic variants of a female with unknown causes of infertility. Methods: Clinical assessment was performed for the phenotype diagnosis. Whole-exome sequencing (WES) and the followed cDNA-PCR sequencing were applied to identify the pathogenic variant and investigate the potentially aberrant mRNA splicing event. The pathogenicity of the variant was analysed using multiple in silico prediction tools, including the 3D protein remodelling. Quantitative RT-PCR (qRT-PCR) was performed to measure PATL2 mRNA expression in the peripheral blood leukocytes of the proband and controls. Results: The proband was diagnosed with the female infertility due to oocyte germinal vesicle (GV) arrest. A novel homozygous splice site variant of PATL2 (NM&#95;001145112.2, c.871-1G>A), inherited from her asymptomatic heterozygous parents, was detected by WES. Sequencing of cDNA amplification products demonstrated that this variant resulted in the exon 10 skipping and in-frame loss of 54 nucleotides in the PATL2 transcript. Quantitative RT-PCR suggested that the mutant transcript escape the mRNA degradation. Conclusion: We identified a novel pathogenic homozygous splice site of PATL2 (c.871-1G>A) underlying the oocyte GV arrest phenotype and elucidated its molecular mechanism. This study expands the variant spectrum of PATL2 and benefits our understanding of its genotype-phenotype correlations., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Sun, Tong, Liu, Tian, Yang, Zhou, Liu, Huang and Li.)

Purpose: Expanded carrier screening (ECS) is an effective method to identify at-risk couples (ARCs) and avoid birth defects. This study aimed to reveal the carrier spectrum in the Chinese population and to delineate an expanded carrier gene panel suitable in China. Methods: Medical exome sequencing (MES), including 4,158 disease-causing genes, was offered to couples at two reproductive centers. It was initially used as a diagnostic yield for potential patients and then used for ECS. Clinical information and ECS results were retrospectively collected. Results: A total of 2,234 couples, representing 4,468 individuals, underwent MES. In total, 254 individuals showed genetic disease symptoms, and 56 of them were diagnosed with genetic diseases by MES. Overall, 94.5% of them were carriers of at least one disease-causing variant. The most prevalent genes were GJB2 for autosomal recessive disorders and G6PD for X-linked diseases. The ARC rate was 9.80%, and couples were inclined to undergo preimplantation genetic testing when diseases were classified as "profound" or "severe." Conclusion: This study provided insight to establish a suitable ECS gene panel for the Chinese population. Disease severity significantly influenced reproductive decision-making. The results highlighted the importance of conducting ECS for couples before undergoing assisted reproductive technology., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Tong, He, He, Li, Hu, Hu, Lu, Lin, Dong, Zhang, Du and Liu.)

Obesity is increasingly prevalent globally, searching for therapeutic agents acting on adipose tissue is of great importance. Equisetin (EQST), a meroterpenoid isolated from a marine sponge-derived fungus, has been reported to display antibacterial and antiviral activities. Here, we revealed that EQST displayed anti-obesity effects acting on adipose tissue through inhibiting adipogenesis in vitro and attenuating HFD-induced obesity in mice, doing so without affecting food intake, blood pressure or heart rate. We demonstrated that EQST inhibited the enzyme activity of 11 β -hydroxysteroid dehydrogenase type 1 (11 β -HSD1), a therapeutic target of obesity in adipose tissue. Anti-obesity properties of EQST were all offset by applying excessive 11 β -HSD1's substrates and 11 β -HSD1 inhibition through knockdown in vitro or 11 β -HSD1 knockout in vivo . In the 11 β -HSD1 bypass model constructed by adding excess 11 β -HSD1 products, EQST's anti-obesity effects disappeared. Furthermore, EQST directly bond to 11 β -HSD1 protein and presented remarkable better intensity on 11 β -HSD1 inhibition and better efficacy on anti-obesity than known 11 β -HSD1 inhibitor. Therefore, EQST can be developed into anti-obesity candidate compound, and this study may provide more clues for developing higher effective 11 β -HSD1 inhibitors., (© 2022 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.)

Background: Gut microbiota has been proposed to be related to the pathogenesis of pulmonary diseases such as asthma and lung cancer, according to the gut-lung axis. However, little is known about the roles of gut microbiota in the pathogenesis of bronchopulmonary dysplasia (BPD). This study was designed to investigate the changes of gut microbiota in neonatal mice with BPD., Methods: BPD model was induced through exposure to high concentration of oxygen. Hematoxylin and eosin (H&E) staining was utilized to determine the modeling efficiency. Stool samples were collected from the distal colon for the sequencing of V3-V4 regions of 16S rRNA, in order to analyze the gut microbiota diversity., Results: Alpha diversity indicated that there were no statistical differences in the richness of gut microbiota between BPD model group and control group on day 7, 14 and 21. Beta diversity analysis showed that there were statistical differences in the gut microbiota on day 14 ( R = 0.368, p = 0.021). Linear discriminant analysis effect size (LEfSe) showed that there were 22 markers with statistical differences on day 14 ( p < 0.05), while those on day 7 and 21 were 3 and 4, respectively. Functional prediction analysis showed that the top three metabolic pathways were signal transduction (P FDR = 0.037), glycan biosynthesis and metabolism (P FDR = 0.032), and metabolism of terpenoids and polyketides (P FDR = 0.049)., Conclusions: BPD mice showed disorder of gut microbiota, which may involve specific metabolic pathways in the early stage. With the progression of neonatal maturity, the differences of the gut microbiota between the two groups would gradually disappear., Competing Interests: The authors declare that they have no competing interests., (© 2022 Fan et al.)

Objective: There is an increasing incidence of bronchopulmonary dysplasia (BDP) in preterm infants in China, which is the key issue affecting their survival rate and life quality. This study was performed to better understand the mechanism of protective effect of celecoxib on hyperoxia induced injury. Methods: Hyperoxia BPD model was established using newborn Sprague-Dawley (SD) rats exposed to high O 2 level (85%). Celecoxib treatment was also conducted. Histology of lung tissue samples were analyzed. Functional studies were systematically performed using the lung tissues and A549 cells. Results: Hyperoxia disrupted lung development in SD rats. Celecoxib alleviated the damaged lung development. NF-κB and Aquaporin (AQP) 1 were identified as the pathways in the hyperoxia-induced lung injury. We have shown that hyperoxia activated NF-κB pathway through increased nucleus translocation and repressed AQP1 expression. On the contrary, celecoxib inhibited NF-κB phosphorylation and nucleus translocation and increased AQP1 expression through inhibiting COX2 activity. Additionally, celecoxib also rescued apoptosis induced by hyperoxia. Conclusion: Our study identified NF-κB and AQP1 as the pathways in the hyperoxia-induced lung injury in the hyperoxia BPD model SD rats and it provided a better understanding of the protective effect of celecoxib. It suggests NF-κB and AQP1 may be as potential targets for treating newborns with BPD.

Objective: Bronchopulmonary dysplasia (BPD) is a severe complication of extreme prematurity that can be caused by hyperoxia inhalation. SP-B and TGF-β have been reported to be implicated in the development of lung. This study aimed to reveal the spatial and temporal expression patterns of these two factors in an animal model of BPD., Methods: Newborn Sprague-Dawley (SD) rats were subjected to hyperoxia conditions to establish an animal model of BPD. The levels of SP-B, TGF-β, MDA and TAOC, as well as the activations of MAPK and PI3K/AKT pathways in lung tissues were monitored during newborn rats prolonged exposure to hyperoxia., Results: We found that hyperoxia exposure significantly induced body weight loss of SD rats. H&E staining for morphometric analyses revealed that hyperoxia arrested alveolar development or loss of alveoli, with fewer and dysmorphic capillaries. mRNA and protein levels of SP-B and TGF-β were high expressed in hyperoxic lung tissues. The concentrations of SP-B and TGF-β in bronchoalveolar lavage fluid were also increased. All these increases begin at the 3th day of hyperoxia exposure. MDA content was increased while TAOC content was decreased in response to hyperoxia. Furthermore, hyperoxia activated p38, and deactivated PI3K and AKT expression., Conclusion: Our research demonstrated that SP-B and TGF-β1 were highly expressed in three levels: mRNA and protein levels in lung tissues, and the release of SP-B and TGF-β1 in bronchoalveolar lavage fluid, beginning at the 3th day of hyperoxia exposure., Competing Interests: None., (IJCEP Copyright © 2018.)