1. Natural Infection of Maize by Cucumber Mosaic Virus in China.
- Author
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Wang, Rong, Wang, Nian, Ye, Ting, Chen, Hui, Fan, Zaifeng, and Zhou, Tao
- Subjects
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CORN diseases , *CUCUMBER mosaic virus , *MOTTLE-leaf , *REVERSE transcriptase polymerase chain reaction , *ENZYME-linked immunosorbent assay , *NUCLEOTIDE sequence - Abstract
In July 2012, symptoms of irregular mosaic stripe and mottle were observed on maize leaves in field in Beijing, China. The causal pathogen was identified to be Cucumber mosaic virus ( CMV) based upon reverse transcription- PCR, enzyme-linked immunosorbent assay, Western blotting and fulfilment of Koch's postulates. The isolate was named ZMBJ- CMV. Full sequence of ZMBJ- CMV RNA3 was determined, and it had the highest identity to that of strain K- CMV (95.03%) and SD- CMV (94.96%). Phylogenetic analysis revealed ZMBJ- CMV clustered with K- CMV and SD- CMV in subgroup IB. To our knowledge, this is the first report on the natural infection and phylogenetic analysis of CMV on maize in China. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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