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Aboveground biomass (AGB) of shrubs and low-statured trees constitutes a substantial portion of the total carbon pool in temperate forest ecosystems, contributing much to local biodiversity, altering tree-regeneration growth rates, and determining above- and belowground food webs. Accurate quantification of AGB at the shrub layer is crucial for ecological modeling and still remains a challenge. Several methods for estimating understory biomass, including inventory and remote sensing-based methods, need to be evaluated against measured datasets. In this study, we acquired 158 individual terrestrial laser scans (TLS) across 45 sites in the Yanshan Mountains and generated metrics including leaf area and stem volume from TLS data using voxel- and non-voxel-based approaches in both leaf-on and leaf-off scenarios. Allometric equations were applied using field-measured parameters as an inventory approach. The results indicated that allometric equations using crown area and height yielded results with higher accuracy than other inventory approach parameters (R2 and RMSE ranging from 0.47 to 0.91 and 12.38 to 38.11 g, respectively). The voxel-based approach using TLS data provided results with R2 and RMSE ranging from 0.86 to 0.96 and 6.43 to 21.03 g. Additionally, the non-voxel-based approach provided similar or slightly better results compared to the voxel-based approach (R2 and RMSE ranging from 0.93 to 0.96 and 4.23 to 11.27 g, respectively) while avoiding the complexity of selecting the optimal voxel size that arises during voxelization. [ABSTRACT FROM AUTHOR]

Background and aims: Dissolved organic nitrogen (DON) has been increasingly recognized as a crucial component of the terrestrial nitrogen (N) cycle that regulates the ecosystem feedback to climate change. Yet, little information is available about the factors that control soil DON in the alpine ecosystems of the Tibetan Plateau, a region that is extremely sensitive to climate change. Methods: Here, we examined the relationship between DON and climate, plant, and soil attributes along a precipitation gradient in five alpine ecosystems (alpine wetland, alpine meadow, alpine shrub, alpine steppe, and alpine desert) across 20 sites that were up to 4000 km apart on the Tibetan Plateau. Results: Results showed that soil DON concentration varied significantly across these alpine ecosystems, and had a positive relationship with mean annual precipitation. Belowground biomass, soil moisture, micro biomass carbon (MBC), and soil total phosphorus (TP) explained 84% of the variation in DON concentrations. The percentage of DON in soil total dissolved N (TDN) varied with vegetation type, with the lowest percentage observed in the alpine meadow (52%) and the highest in the alpine desert (77%), indicating the slow turnover of DON into inorganic N in systems with low litter input and microbial activity. Conclusions: Precipitation and plant biomass input determine the concentration and turnover of soil DON on the Tibetan Plateau. A warmer and wetter climate that has been predicted for the Tibetan grasslands may lead to a larger and more active DON pool. [ABSTRACT FROM AUTHOR]

In the alpine steppe zone on the Central Tibetan Plateau, a large amount of area has been degraded due to natural and artificial factors. N & P fertilization is widely accepted to recover degraded pastures in other regions all over the world. However, it is not clear how alpine steppe communities respond to N & P fertilization, and what is the optimal application rate, in the perspective of forage production. To attempt to explore these questions, in July 2013, two fencing sites were designed in Baingoin County with 12 treatments of different levels of nitrogen (N0: 0; N1: 7.5 g m-2 yr-1; N2: 15 g m-2 yr-1) & phosphate (P0: 0; P1: 7.5 gP2O5 m-2 yr-1; P2: 15 gP2O5 m-2 yr-1; P3: 30 gP2O5 m-2 yr-1). The results indicated N&P addition was capable to ameliorate the quality of the two sites in the Tibetan Plateau steppe. Increasing N application level resulted in significant increment in Gramineae and total biomass in the two sites. P addition significantly improved the quantity of Compositae, total biomass and the biomasss of other species in site II, while it only significantly improved the total biomass in site I. Gramineae was much more sensitive to N-induced changes than P-induced changes, and this indicated N addition was better to ameliorate the quality of plateau steppe than P-induced changes. No strong evidence was found for critical threshold within 15 g N m-2 yr-1, and there was decreasing tendency when P addition rate was above 15 g m-2 yr-1. N&P has the potential to accelerate soil acidification, which improved the content of available K, likely as a result of nonsignificant correlation between biomass and soil moisture. This work highlights the the tradeoffs that exist in N and P addition in recovering degraded steppe. [ABSTRACT FROM AUTHOR]