7,395 results on '"Luo, An"'
Search Results
2. CsBZR1 Family Transcription Factors in Wild and Cultural Tea Plants and Their Response to Hormone and Abiotic Stress
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Li, Jing-Wen, Zhou, Ping, Yang, Ni, Hu, Zhi-Hang, Chen, Yi, Luo, Wei, Kong, Jie-Yu, Qin, Zhi-Yuan, Li, Xing-Hui, Chen, Xuan, and Zhuang, Jing
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- 2024
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3. Glycerophosphoinositol modulates FGA and NOTCH3 in exercise-induced muscle adaptation and colon cancer progression
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Hongbiao Luo, Wei Luo, Ning Ding, Huimin Zhu, Jiahui Lai, Qingzhu Tang, and Yongheng He
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FGA ,Notch3 ,PTMs ,colon cancer ,prognosis ,gene expression ,Therapeutics. Pharmacology ,RM1-950 - Abstract
ObjectivesFibroleukin (FGA) and NOTCH3 are vital in both exercise-induced muscle adaptation and colon adenocarcinoma (COAD) progression. This study aims to elucidate the roles of FGA and NOTCH3 in phenotypic variations of striated muscle induced by exercise and in COAD development. Additionally, it seeks to evaluate the prognostic significance of these proteins.MethodsGene Set Variation Analysis (GSVA) and protein-protein interaction (PPI) network analysis were employed to identify differentially expressed genes (DEGs). Molecular docking studies were conducted to assess the binding affinities of 39 compounds to the NOTCH3 protein. In vitro assays, including mobileular viability, gene expression, and apoptosis assays, were performed to evaluate the effects of glycerophosphoinositol on FGA and NOTCH3 expression. Additionally, copy number variation (CNV), methylation status, and survival analyses were conducted across multiple cancers types.ResultsThe NOTCH signaling pathway was consistently upregulated in exercise-induced muscle samples. High NOTCH3 expression was associated with poor prognosis in COAD, extracellular matrix organization, immune infiltration, and activation of the PI3K-Akt pathway. Molecular docking identified gamma-Glu-Trp, gamma-Glutamyltyrosine, and 17-Deoxycortisol as strong binders to NOTCH3. Glycerophosphoinositol treatment modulated FGA and NOTCH3 expression, influencing cell proliferation and apoptosis. CNV and methylation analyses revealed specific changes in FGA and NOTCH3 across 20 cancers types. Survival analyses showed strong associations between FGA/NOTCH3 expression and survival metrics, with negative correlations for FGA and positive correlations for NOTCH3.ConclusionFGA and NOTCH3 play significant roles in exercise-induced muscle adaptation and colon cancer progression. The expression profiles and interactions of these proteins provide promising prognostic markers and therapeutic targets. These findings offer valuable insights into the post-translational modifications (PTMs) in human cancer, highlighting novel pharmacological and therapeutic opportunities.
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- 2024
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4. Isolation and expression analysis of cellulose synthase 3 (Ces3) genes from sugarcane (Saccharum officinarum L.)
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Cheng-Mei Huang, Kai-Chao Wu, Krishan K. Verma, Hai-Bin Luo, Zhi-Nian Deng, Guo-Ying Shi, Hui-Qing Cao, Xing-Jian Wu, Li-Ping Ye, Yuan-Wen Wei, and Lin Xu
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Gene expression ,DEGs ,SoCes3 ,Transgenic tobacco ,Saccharum officinarum L. ,Agriculture - Abstract
Abstract Plant cellulose synthase is one of the important glycosyltransferases, which catalyzes the synthesis of the paracrystalline of H-bonded-β-(1,4)-glucose chains. This study isolated the cellulose synthase 3 (Ces3) sequence from sugarcane (Saccharum officinarum L.) leaves. SoCes3 (GenBank accession No. MG324347) has a full-length cDNA sequence of 3625 bp. It contains an open reading frame (3225 bp), encoding 1074 amino acids with a molecular weight of about 120.89 kDa and isoelectric point of 6.26. SoCes3 protein showed high activity with other plant cellulose synthases. The recombinant protein contains plant cellulose synthase (Ces) protein conservative domains. In subcellular localization experiments, the fusion protein of SoCes3 with green fluorescent protein (GFP) was specifically localized in the cell membrane. The gene expression of SoCes3 was found in the leaf, leaf sheath, and internodes of the sugarcane stem. The highest expression level was found in the internode, especially with the highest expression level in the 5th internode and lowest in the leaves, and the gene expression level of SoCes3 was upregulated by PP333 and not in gibberellic acid-treated plants. It was conducted in tobacco plants to understand the biotechnological potential of SoCes3. The contents of cellulose and lignin were increased in SoCes3-overexpressing tobacco. Transcriptomic analysis showed that the transgenic tobacco induced different genes associated with different biological regulatory processes. Differentially expressed genes (DEGs) mediated plant hormone signal transduction, starch and sucrose metabolism signaling pathways were widely induced and mostly upregulated. The transcription levels in SoCes3-overexpressing transgenic lines were higher than wild-type. Graphical Abstract
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- 2024
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5. Identification of NINJ1 as a novel prognostic predictor for retroperitoneal liposarcoma
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Yu Zhao, Da Qin, Xiangji Li, Tiange Wang, Tong Zhang, Xiaosong Rao, Li Min, Zhiyi Wan, Chenghua Luo, and Mengmeng Xiao
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NINJ1 ,Prognosis ,Retroperitoneal liposarcoma ,Gene expression ,Overall survival ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Retroperitoneal liposarcoma (RPLS) is known for its propensity for local recurrence and short survival time. We aimed to identify a credible and specific prognostic biomarker for RPLS. Methods Cases from The Cancer Genome Atlas (TCGA) sarcoma dataset were included as the training group. Co-expression modules were constructed using weighted gene co-expression network analysis (WGCNA) to explore associations between modules and survival. Survival analysis of hub genes was performed using the Kaplan–Meier method. In addition, independent external validation was performed on a cohort of 135 Chinese RPLS patients from the REtroperitoneal SArcoma Registry (RESAR) study (NCT03838718). Results A total of 19 co-expression modules were constructed based on the expression levels of 26,497 RNAs in the TCGA cohort. Among these modules, the green module exhibited a positive correlation with overall survival (OS, p = 0.10) and disease-free survival (DFS, p = 0.06). Gene set enrichment analysis showed that the green module was associated with endocytosis and soft-tissue sarcomas. Survival analysis demonstrated that NINJ1, a hub gene within the green module, was positively associated with OS (p = 0.019) in the TCGA cohort. Moreover, in the validation cohort, patients with higher NINJ1 expression levels displayed a higher probability of survival for both OS (p = 0.023) and DFS (p = 0.012). Multivariable Cox analysis further confirmed the independent prognostic significance of NINJ1. Conclusions We here provide a foundation for the establishment of a consensus prognostic biomarker for RPLS, which should not only facilitate medical treatment but also guide the development of novel targeted drugs.
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- 2024
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6. Systematic characterization of Gossypium GLN family genes reveals a potential function of GhGLN1.1a regulates nitrogen use efficiency in cotton
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Xiaotong Li, Yunqi Gu, Mirezhatijiang Kayoumu, Noor Muhammad, Xiangru Wang, Huiping Gui, Tong Luo, Qianqian Wang, Xieraili Wumaierjiang, Sijia Ruan, Asif Iqbal, Xiling Zhang, Meizhen Song, and Qiang Dong
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Gossypium ,Glutamine synthetase ,Gene family ,Gene expression ,Nitrogen use efficiency ,Botany ,QK1-989 - Abstract
Abstract The enzyme glutamine synthetase (GLN) is mainly responsible for the assimilation and reassimilation of nitrogen (N) in higher plants. Although the GLN gene has been identified in various plants, there is little information about the GLN family in cotton (Gossypium spp.). To elucidate the roles of GLN genes in cotton, we systematically investigated and characterized the GLN gene family across four cotton species (G. raimondii, G. arboreum, G. hirsutum, and G. barbadense). Our analysis encompassed analysis of members, gene structure, cis-element, intragenomic duplication, and exploration of collinear relationships. Gene duplication analysis indicated that segmental duplication was the primary driving force for the expansion of the GhGLN gene family. Transcriptomic and quantitative real-time reverse-transcription PCR (qRT-PCR) analyses indicated that the GhGLN1.1a gene is responsive to N induction treatment and several abiotic stresses. The results of virus-induced gene silencing revealed that the accumulation and N use efficiency (NUE) of cotton were affected by the inactivation of GhGLN1.1a. This study comprehensively analyzed the GhGLN genes in Gossypium spp., and provides a new perspective on the functional roles of GhGLN1.1a in regulating NUE in cotton.
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- 2024
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7. Circadian rhythm response and its effect on photosynthetic characteristics of the Lhcb family genes in tea plant
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Zhi-Hang Hu, Nan Zhang, Zhi-Yuan Qin, Jing-Wen Li, Jian-Ping Tao, Ni Yang, Yi Chen, Jie-Yu Kong, Wei Luo, Xuan Chen, Xing-Hui Li, Ai-Sheng Xiong, and Jing Zhuang
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Camellia sinensis ,CsLhcb ,Circadian clock ,Photosynthetic parameters ,Gene expression ,Botany ,QK1-989 - Abstract
Abstract Background The circadian clock, also known as the circadian rhythm, is responsible for predicting daily and seasonal changes in the environment, and adjusting various physiological and developmental processes to the appropriate times during plant growth and development. The circadian clock controls the expression of the Lhcb gene, which encodes the chlorophyll a/b binding protein. However, the roles of the Lhcb gene in tea plant remain unclear. Results In this study, a total of 16 CsLhcb genes were identified based on the tea plant genome, which were distributed on 8 chromosomes of the tea plant. The promoter regions of CsLhcb genes have a variety of cis-acting elements including hormonal, abiotic stress responses and light response elements. The CsLhcb family genes are involved in the light response process in tea plant. The photosynthetic parameter of tea leaves showed rhythmic changes during the two photoperiod periods (48 h). Stomata are basically open during the day and closed at night. Real-time quantitative PCR results showed that most of the CsLhcb family genes were highly expressed during the day, but were less expressed at night. Conclusions Results indicated that CsLhcb genes were involved in the circadian clock process of tea plant, it also provided potential references for further understanding of the function of CsLhcb gene family in tea plant.
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- 2024
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8. Whole genome identification, molecular docking and expression analysis of enzymes involved in the selenomethionine cycle in Cardamine hupingshanensis
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Xixi Zeng, Guoqiang Luo, Zhucheng Fan, Zhijing Xiao, Yanke Lu, Qiang Xiao, Zhi Hou, Qiaoyu Tang, and Yifeng Zhou
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Cardamine Hupingshanensis ,Selenomethionine cycle ,Molecular docking ,Gene expression ,Selenium stress response ,Botany ,QK1-989 - Abstract
Abstract Background The selenomethionine cycle (SeMTC) is a crucial pathway for the metabolism of selenium. The basic bioinformatics and functions of four enzymes involved in the cycle including S-adenosyl-methionine synthase (MAT), SAM-dependent methyltransferase (MTase), S-adenosyl-homocysteine hydrolase (SAHH) and methionine synthase (MTR), have been extensively reported in many eukaryotes. The identification and functional analyses of SeMTC genes/proteins in Cardamine hupingshanensis and their response to selenium stress have not yet been reported. Results In this study, 45 genes involved in SeMTC were identified in the C. hupingshanensis genome. Phylogenetic analysis showed that seven genes from ChMAT were clustered into four branches, twenty-seven genes from ChCOMT were clustered into two branches, four genes from ChSAHH were clustered into two branches, and seven genes from ChMTR were clustered into three branches. These genes were resided on 16 chromosomes. Gene structure and homologous protein modeling analysis illustrated that proteins in the same family are relatively conserved and have similar functions. Molecular docking showed that the affinity of SeMTC enzymes for selenium metabolites was higher than that for sulfur metabolites. The key active site residues identified for ChMAT were Ala269 and Lys273, while Leu221/231 and Gly207/249 were determined as the crucial residues for ChCOMT. For ChSAHH, the essential active site residues were found to be Asn87, Asp139 and Thr206/207/208/325. Ile204, Ser111/329/377, Asp70/206/254, and His329/332/380 were identified as the critical active site residues for ChMTR. In addition, the results of the expression levels of four enzymes under selenium stress revealed that ChMAT3-1 genes were upregulated approximately 18-fold, ChCOMT9-1 was upregulated approximately 38.7-fold, ChSAHH1-2 was upregulated approximately 11.6-fold, and ChMTR3-2 genes were upregulated approximately 28-fold. These verified that SeMTC enzymes were involved in response to selenium stress to varying degrees. Conclusions The results of this research are instrumental for further functional investigation of SeMTC in C. hupingshanensis. This also lays a solid foundation for deeper investigations into the physiological and biochemical mechanisms underlying selenium metabolism in plants.
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- 2024
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9. Functional characterization of D-type cyclins involved in cell division in rice
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Zhishu Jiang, Xin Wang, Zhiwei Zhou, Limei Peng, Xiaoli Lin, Xiaowei Luo, Yongping Song, Huaying Ning, Cong Gan, Xiaopeng He, Changlan Zhu, Linjuan Ouyang, Dahu Zhou, Yicong Cai, Jie Xu, Haohua He, and Yantong Liu
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Rice ,D-type cyclin ,Cell cycle ,Gene expression ,Protein interaction ,Botany ,QK1-989 - Abstract
Abstract Background D-type cyclins (CYCD) regulate the cell cycle G1/S transition and are thus closely involved in cell cycle progression. However, little is known about their functions in rice. Results We identified 14 CYCD genes in the rice genome and confirmed the presence of characteristic cyclin domains in each. The expression of the OsCYCD genes in different tissues was investigated. Most OsCYCD genes were expressed at least in one of the analyzed tissues, with varying degrees of expression. Ten OsCYCD proteins could interact with both retinoblastoma-related protein (RBR) and A-type cyclin-dependent kinases (CDKA) forming holistic complexes, while OsCYCD3;1, OsCYCD6;1, and OsCYCD7;1 bound only one component, and OsCYCD4;2 bound to neither protein. Interestingly, all OsCYCD genes except OsCYCD7;1, were able to induce tobacco pavement cells to re-enter mitosis with different efficiencies. Transgenic rice plants overexpressing OsCYCD2;2, OsCYCD6;1, and OsCYCD7;1 (which induced cell division in tobacco with high-, low-, and zero-efficiency, respectively) were created. Higher levels of cell division were observed in both the stomatal lineage and epidermal cells of the OsCYCD2;2- and OsCYCD6;1-overexpressing plants, with lower levels seen in OsCYCD7;1-overexpressing plants. Conclusions The distinct expression patterns and varying effects on the cell cycle suggest different functions for the various OsCYCD proteins. Our findings will enhance understanding of the CYCD family in rice and provide a preliminary foundation for the future functional verification of these genes.
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- 2024
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10. Integrated metabolome and transcriptome analyses reveal the molecular mechanism underlying dynamic metabolic processes during taproot development of Panax notoginseng
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Xuejiao Li, Yan Zhao, Shuilian He, Jing Meng, Yingchun Lu, Huineng Shi, Chunlan Liu, Bing Hao, Qingyan Tang, Shuangyan Zhang, Guanghui Zhang, Yu Luo, Shengchao Yang, Jianli Yang, and Wei Fan
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Panax notoginseng ,Taproot development ,Metabolic changes ,Gene expression ,Triterpenoid saponins ,Botany ,QK1-989 - Abstract
Abstract Background Panax notoginseng (Burk) F. H. Chen is one of the most famous Chinese traditional medicinal plants. The taproot is the main organ producing triterpenoid saponins, and its development is directly linked to the quality and yield of the harvested P. notoginseng. However, the mechanisms underlying the dynamic metabolic changes occurring during taproot development of P. notoginseng are unknown. Results We carried out metabolomic and transcriptomic analyses to investigate metabolites and gene expression during the development of P. notoginseng taproots. The differentially accumulated metabolites included amino acids and derivatives, nucleotides and derivatives, and lipids in 1-year-old taproots, flavonoids and terpenoids in 2- and 3-year-old taproots, and phenolic acids in 3-year-old taproots. The differentially expressed genes (DEGs) are related to phenylpropanoid biosynthesis, metabolic pathway and biosynthesis of secondary metabolites at all three developmental stages. Integrative analysis revealed that the phenylpropanoid biosynthesis pathway was involved in not only the development of but also metabolic changes in P. notoginseng taproots. Moreover, significant accumulation of triterpenoid saponins in 2- and 3-year-old taproots was highly correlated with the up-regulated expression of cytochrome P450s and uridine diphosphate-dependent glycosyltransferases genes. Additionally, a gene encoding RNase-like major storage protein was identified to play a dual role in the development of P. notoginseng taproots and their triterpenoid saponins synthesis. Conclusions These results elucidate the molecular mechanism underlying the accumulation of and change relationship between primary and secondary metabolites in P. notoginseng taproots, and provide a basis for the quality control and genetic improvement of P. notoginseng.
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- 2024
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11. Effect of light intensity on the accumulation of protostane triterpenes in Asian water plantain (Alisma orientale)
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Wu, Wenqing, Li, Simeng, Gu, Wei, Tian, Rong, Luo, Man, Tang, Junjie, Tang, Min, and Wu, Yinzhi
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- 2024
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12. Effect of capsular polysaccharide phase variation on biofilm formation, motility and gene expression in Vibrio vulnificus
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Zhang, Tingting, Ji, Shenjie, Zhang, Miaomiao, Wu, Fei, Li, Xue, Luo, Xi, Huang, Qinglian, Li, Min, Zhang, Yiquan, and Lu, Renfei
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Gene expression ,Polysaccharides ,Genes ,RNA ,Health - Abstract
Vibrio vulnificus, a significant marine pathogen, undergoes opaque (Op)-translucent (Tr) colony switching based on whether capsular polysaccharide (CPS) is produced. CPS phase variation is sometime accompanied by genetic variation or down-regulation of particular genes, such as wzb. In addition, CPS prevents biofilm formation and is important to the virulence of V. vulnificus. However, the extent to which there is a difference in gene expression between Tr and Op colonies and the impact of CPS phase variation on other behaviors of V. vulnificus remain unknown. In this work, the data have shown that CPS phase variation of V. vulnificus is affected by incubation time. Tr and Op strains exhibited similar growth rates. However, Tr strains had enhanced biofilm formation capacities but reduced swimming motility compared to Op strains. The RNA-seq assay revealed 488 differentially expressed genes, with 214 downregulated and 274 upregulated genes, between Tr and Op colonies. Genes associated with Tad pili and CPS were downregulated, whereas those involved in flagellum were upregulated, in Tr colonies compared with Op colonies. In addition, 9 putative c-di-GMP metabolism-associated genes and 28 genes encoding putative regulators were significantly differentially expressed, suggesting that CPS phase variation is probably strictly regulated in V. vulnificus. Moreover, 8 genes encoding putative porins were also differentially expressed between the two phenotypic colonies, indicating that bacterial outer membrane was remodeled during CPS phase variation. In brief, this work highlighted the gene expression profiles associated with CPS phase variation, but more studies should be performed to disclose the intrinsic mechanisms in the future. Keywords: Vibrio vulnificus; CPS phase variation, Biofilm, Motility, Regulation, Author(s): Tingting Zhang[sup.1,2], Shenjie Ji[sup.3], Miaomiao Zhang[sup.1], Fei Wu[sup.1], Xue Li[sup.1], Xi Luo[sup.1], Qinglian Huang[sup.1,2,3], Min Li[sup.4], Yiquan Zhang[sup.1] and Renfei Lu[sup.1,2] Introduction Vibrio vulnificus is a Gram-negative, halophilic bacterium [...]
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- 2024
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13. Environmental magnesium ion affects global gene expression, motility, biofilm formation and virulence of Vibrio parahaemolyticus
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Xue Li, Xiaobai Zhang, Miaomiao Zhang, Xi Luo, Tingting Zhang, Xianjin Liu, Renfei Lu, and Yiquan Zhang
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Vibrio parahaemolyticus ,Mg2+ ,Motility ,Biofilm ,Virulence ,Gene expression ,Biotechnology ,TP248.13-248.65 ,Microbiology ,QR1-502 - Abstract
Vibrio parahaemolyticus is widely distributed in marine ecosystems. Magnesium ion (Mg2+) is the second most abundant metal cation in seawater, and plays important roles in the growth and gene expression of V. parahaemolyticus, but lacks the detailed mechanisms. In this study, the RNA sequencing data demonstrated that a total of 1494 genes was significantly regulated by Mg2+. The majority of the genes associated with lateral flagella, exopolysaccharide, type III secretion system 2, type VI secretion system (T6SS) 1, T6SS2, and thermostable direct hemolysin were downregulated. A total of 18 genes that may be involved in c-di-GMP metabolism and more than 80 genes encoding putative regulators were also significantly and differentially expressed in response to Mg2+, indicating that the adaptation process to Mg2+ stress may be strictly regulated by complex regulatory networks. In addition, Mg2+ promoted the proliferative speed, swimming motility and cell adhesion of V. parahaemolyticus, but inhibited the swarming motility, biofilm formation, and c-di-GMP production. However, Mg2+ had no effect on the production of capsular polysaccharide and cytoxicity against HeLa cells. Therefore, Mg2+ had a comprehensive impact on the physiology and gene expression of V. parahaemolyticus.
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- 2024
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14. Transcriptomic analyses and machine-learning methods reveal dysregulated key genes and potential pathogenesis in human osteoarthritic cartilage
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Di Zhao, Ling-feng Zeng, Gui-hong Liang, Ming-hui Luo, Jian-ke Pan, Yao-xing Dou, Fang-zheng Lin, He-tao Huang, Wei-yi Yang, and Jun Liu
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osteoarthritis ,cartilage ,transcription ,machine learning ,dysregulated key genes ,pathogenesis ,osteoarthritic cartilage ,cartilage tissue ,rnas ,inflammation ,biomarkers ,osteoarthritis (oa) ,gene expression ,articular cartilage degradation ,gene expression profiles ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Aims: This study aimed to explore the biological and clinical importance of dysregulated key genes in osteoarthritis (OA) patients at the cartilage level to find potential biomarkers and targets for diagnosing and treating OA. Methods: Six sets of gene expression profiles were obtained from the Gene Expression Omnibus database. Differential expression analysis, weighted gene coexpression network analysis (WGCNA), and multiple machine-learning algorithms were used to screen crucial genes in osteoarthritic cartilage, and genome enrichment and functional annotation analyses were used to decipher the related categories of gene function. Single-sample gene set enrichment analysis was performed to analyze immune cell infiltration. Correlation analysis was used to explore the relationship among the hub genes and immune cells, as well as markers related to articular cartilage degradation and bone mineralization. Results: A total of 46 genes were obtained from the intersection of significantly upregulated genes in osteoarthritic cartilage and the key module genes screened by WGCNA. Functional annotation analysis revealed that these genes were closely related to pathological responses associated with OA, such as inflammation and immunity. Four key dysregulated genes (cartilage acidic protein 1 (CRTAC1), iodothyronine deiodinase 2 (DIO2), angiopoietin-related protein 2 (ANGPTL2), and MAGE family member D1 (MAGED1)) were identified after using machine-learning algorithms. These genes had high diagnostic value in both the training cohort and external validation cohort (receiver operating characteristic > 0.8). The upregulated expression of these hub genes in osteoarthritic cartilage signified higher levels of immune infiltration as well as the expression of metalloproteinases and mineralization markers, suggesting harmful biological alterations and indicating that these hub genes play an important role in the pathogenesis of OA. A competing endogenous RNA network was constructed to reveal the underlying post-transcriptional regulatory mechanisms. Conclusion: The current study explores and validates a dysregulated key gene set in osteoarthritic cartilage that is capable of accurately diagnosing OA and characterizing the biological alterations in osteoarthritic cartilage; this may become a promising indicator in clinical decision-making. This study indicates that dysregulated key genes play an important role in the development and progression of OA, and may be potential therapeutic targets. Cite this article: Bone Joint Res 2024;13(2):66–82.
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- 2024
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15. PbrWRKY62-PbrADC1 module involves in superficial scald development of Pyrus bretschneideri Rehd.fruit via regulating putrescine biosynthesis
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Xu Zhang, Lijuan Zhu, Ming Qian, Li Jiang, Peng Gu, Luting Jia, Chunlu Qian, Weiqi Luo, Min Ma, Zhangfei Wu, Xin Qiao, Libin Wang, and Shaoling Zhang
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P. bretschneideri Rehd. ,Superficial scald development ,Putrescine biosynthesis ,PbrWRKY62-PbrADC1 module ,Gene expression ,Metabolomics ,Plant culture ,SB1-1110 ,Botany ,QK1-989 - Abstract
Abstract Putrescine plays a role in superficial scald development during the cold storage of pear fruit. However, the molecular mechanism behind this phenomenon has not been un-fully clarified until recently. In this study, a conjoint analysis of metabolites and gene expression profiles in the putrescine-metabolic pathway of P. bretschneideri Rehd. fruit followed by experimental validation revealed that PbrADC1, forming a homodimer in the chloroplast, was involved in putrescine biosynthesis and thus fruit chilling resistance. Additionally, the substrate-binding residue Cys546 in PbrADC1, whose activity was modified by H2O2, played a crucial role in arginine decarboxylation into agmatine. Through a combined analysis of the distribution of cis-acting elements in the PbrADC1 promoter as well as the expression profiles of related transcription factors (TFs), several TFs were identified as upstream regulators of PbrADC1 gene. Further investigation revealed that the nuclear PbrWRKY62 could directly bind to the W-box elements in the PbrADC1 promoter, activate its expression, enhance putrescine accumulation, and thus increase fruit chilling tolerance. In conclusion, our results suggest that the PbrWRKY62-PbrADC1 module is involved in the development of superficial scald in P. bretschneideri Rehd. fruit via regulating putrescine biosynthesis. Consequently, these findings could serve as valuable genetic resources for breeding scald-resistant pear fruit. Graphical Abstract
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- 2024
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16. Effects of paclobutrazol seed priming on seedling quality, photosynthesis, and physiological characteristics of fragrant rice
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Yingying Zhang, Zhenzhen He, Pipeng Xing, Haowen Luo, Zhuosheng Yan, and Xiangru Tang
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2-acetyl-1-pyrroline ,BADH2 ,Gene expression ,Botany ,QK1-989 - Abstract
Abstract Background Paclobutrazol is widely used in the agricultural field. This study investigated the effects of seed priming with different concentrations of paclobutrazol on seedling quality, 2-acetyl-1-pyrroline (2-AP, a key aroma component of fragrant rice) biosynthesis, and related physiological and biochemical indicators in fragrant rice seedlings. Results The experiment is being conducted at the College of Agriculture, South China Agricultural University. In the experiment, three concentrations of paclobutrazol (Pac 1: 20 mg·L−1; Pac 2: 40 mg·L−1; Pac 3: 80 mg·L−1) were used to initiate the treatment of fragrant rice seeds, while water treatment was used as a control (CK). The results showed that compared with CK, paclobutrazol treatment reduced plant height, increased stem diameter, and increased fresh and dry weight of aromatic rice seedlings. Moreover, paclobutrazol treatment also increased the seedlings’ photosynthetic pigment content and net photosynthetic rate. Conclusions This study demonstrates that paclobutrazol primarily increases the content of proline by reducing the content of glutamate and down-regulating the expression of P5CS2, thereby promoting the conversion of proline to the aromatic substance 2-AP. Under the appropriate concentration of paclobutrazol (40 mg·L−1~80 mg·L−1), the seedling quality, stress resistance, and aroma of fragrant rice can be improved.
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- 2024
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17. Manganese-superoxide dismutase (MnSOD) rescues redox balance and mucosal barrier function in midgut of hybrid fish (Carassius cuvieri ♀ × Carassius auratus red var ♂) infected with Aeromonas hydrophila and Edwardsiella tarda
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Jie Ou, Wei-Sheng Luo, Zi-Rou Zhong, Qing Xie, Fei Wang, Ning-Xia Xiong, and Sheng-Wei Luo
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Crucian carp ,MnSOD ,Gut immunity ,Gene expression ,Genetics ,QH426-470 ,Reproduction ,QH471-489 ,Animal biochemistry ,QP501-801 - Abstract
MnSOD is a ubiquitous metalloenzyme that constitutes the first line of antioxidant defense against oxidative stress. In this study, full length sequence of MnSOD was identified from hybrid crucian carp (WR, Carassius cuvieri ♀ × Carassius auratus red var ♂). Tissue-specific analysis revealed that the highest expression of WR-MnSOD was detected in kidney. Aeromonas hydrophila challenge could dramatically enhance WR-MnSOD mRNA expression in tissues. In vivo administration of purified WR-MnSOD peptide could maintain gut mucosal barrier function, rescue redox balance as well as decrease apoptotic cell death in midgut upon bacterial infection, suggesting that WR-MnSOD is playing a crucial role in gut mucosal barrier function and could be used as feed additive to improve gut immunity in fish.
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- 2023
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18. Tumor necrosis factor α1 (TNFα1) administration can disrupt barrier function and attenuate redox defense in midgut of red crucian carp (Carassius auratus red var)
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Jin-Fang Huang, Ning-Xia Xiong, Shi-Yun Li, Ke-Xin Li, Jie Ou, Fei Wang, and Sheng-Wei Luo
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Crucian carp ,Cytokines ,Gene expression ,Gut immunity ,Genetics ,QH426-470 ,Reproduction ,QH471-489 ,Animal biochemistry ,QP501-801 - Abstract
TNFα belongs to superfamily of tumor necrosis factor that can exert the pleiotropic effort in a large quantity of biological processes, including immune homeostasis, intracellular modulation and etiopathogenesis, but its regulatory role in mucosal immune regulation of fish is unclear. Currently, this study aimed to evaluate the immunoregulatory function of TNFα1 on gut barrier in teleost fish. In this study, TNFα1 sequences were identified from red crucian carp (RCC, Carassius auratus red var). The high-level expression of RCC-TNFα1 mRNA was detected in gill among all the isolated samples. Then, RCC-TNFα1 expression increased dramatically in immune-related tissues after A. hydrophila infection and in cultured fish cells after lipopolysaccharide (LPS) treatment. RCC-TNFα1 fusion protein was generated and purified in vitro. RCC receiving RCC-TNFα1 perfusion showed an increased levels of villi fusion and edema in injured midgut with the fuzzy appearance. In addition, The mRNA expressions of tight junction (TJ) genes, mucin genes and redox sensitive genes decreased sharply in TNFα1-treated midgut in comparison with those of the control (P
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- 2023
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19. GoM DE: interpreting structure in sequence count data with differential expression analysis allowing for grades of membership
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Carbonetto, Peter, Luo, Kaixuan, Sarkar, Abhishek, Hung, Anthony, Tayeb, Karl, Pott, Sebastian, and Stephens, Matthew
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- 2023
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20. GhCKX14 responding to drought stress by modulating antioxi-dative enzyme activity in Gossypium hirsutum compared to CKX family genes
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Li, Tengyu, Luo, Kun, Wang, Chenlei, Wu, Lanxin, Pan, Jingwen, Wang, Mingyang, Liu, Jinwei, Li, Yan, Yao, Jinbo, Chen, Wei, Zhu, Shouhong, and Zhang, Yongshan
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- 2023
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21. Trans-Zeatin induced regulation of the biosynthesis of 2-acetyl-1-pyrroline in fragrant rice (Oryza sativa L.) seedlings
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Xing, Pipeng, Luo, Haowen, He, Zhenzhen, He, Longxin, Zhao, Hua, Tang, Xiangru, and Duan, Meiyang
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- 2023
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22. Gene expression plasticity followed by genetic change during colonization in a high-elevation environment
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Huishang She, Yan Hao, Gang Song, Xu Luo, Fumin Lei, Weiwei Zhai, and Yanhua Qu
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gene expression ,plasticity ,genetic variation ,tree sparrows ,Medicine ,Science ,Biology (General) ,QH301-705.5 - Abstract
Phenotypic plasticity facilitates organismal invasion of novel environments, and the resultant phenotypic change may later be modified by genetic change, so called ‘plasticity first.’ Herein, we quantify gene expression plasticity and regulatory adaptation in a wild bird (Eurasian Tree Sparrow) from its original lowland (ancestral stage), experimentally implemented hypoxia acclimation (plastic stage), and colonized highland (colonized stage). Using a group of co-expressed genes from the cardiac and flight muscles, respectively, we demonstrate that gene expression plasticity to hypoxia tolerance is more often reversed than reinforced at the colonized stage. By correlating gene expression change with muscle phenotypes, we show that colonized tree sparrows reduce maladaptive plasticity that largely associated with decreased hypoxia tolerance. Conversely, adaptive plasticity that is congruent with increased hypoxia tolerance is often reinforced in the colonized tree sparrows. Genes displaying large levels of reinforcement or reversion plasticity (i.e. 200% of original level) show greater genetic divergence between ancestral and colonized populations. Overall, our work demonstrates that gene expression plasticity at the initial stage of high-elevation colonization can be reversed or reinforced through selection-driven adaptive modification.
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- 2024
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23. Dysregulation of iron transport-related biomarkers in blood leukocytes is associated with poor prognosis of early trauma
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Zhusheng Feng, Yingnan Fan, Xiaofei Shi, Xu Luo, Jiangang Xie, Shanshou Liu, Chujun Duan, Qianmei Wang, Yuqin Ye, and Wen Yin
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Trauma ,Iron transport ,Prognosis ,Leukocyte ,Gene expression ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Objective: The early targeted and effective diagnosis and treatment of severe trauma are crucial for patients' outcomes. Blood leukocytes act as significant effectors during the initial inflammation and activation of innate immunity in trauma. This study aims to identify hub genes related to patients' prognosis in blood leukocytes at the early stages of trauma. Methods: The expression profiles of Gene Expression Omnibus (GEO) Series (GSE) 36809 and GSE11375 were downloaded from the GEO database. R software, GraphPad Prism 9.3.1 software, STRING database, and Cytoscape software were used to process the data and identify hub genes in blood leukocytes of early trauma. Results: Gene Ontology (GO) analysis showed that the differentially expressed genes (DEGs) of blood leukocytes at the early stages of trauma (0–4 h, 4–8 h, and 8–12 h) were mainly involved in neutrophil activation and neutrophil degranulation, neutrophil activation involved in immune response, neutrophil mediated immunity, lymphocyte differentiation, and cell killing. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the DEGs were mainly involved in Osteoclast differentiation and Hematopoietic cell lineage. Sixty-six down-regulated DEGs and 148 up-regulated DEGs were identified and 37 hub genes were confirmed by Molecular Complex Detection (MCODE) of Cytoscape. Among the hub genes, Lipocalin 2 (LCN2), Lactotransferrin (LTF), Olfactomedin 4 (OLFM4), Resistin (RETN), and Transcobalamin 1 (TCN1) were related to prognosis and connected with iron transport closely. LCN2 and LTF were involved in iron transport and had a moderate predictive value for the poor prognosis of trauma patients, and the AUC of LCN2 and LTF was 0.7777 and 0.7843, respectively. Conclusion: As iron transport-related hub genes in blood leukocytes, LCN2 and LTF can be used for prognostic prediction of early trauma.
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- 2024
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24. GoM DE: interpreting structure in sequence count data with differential expression analysis allowing for grades of membership
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Peter Carbonetto, Kaixuan Luo, Abhishek Sarkar, Anthony Hung, Karl Tayeb, Sebastian Pott, and Matthew Stephens
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Gene expression ,Single-cell RNA-seq ,Single-cell ATAC-seq ,Differential expression analysis ,Dimensionality reduction ,Parts-based representations ,Biology (General) ,QH301-705.5 ,Genetics ,QH426-470 - Abstract
Abstract Parts-based representations, such as non-negative matrix factorization and topic modeling, have been used to identify structure from single-cell sequencing data sets, in particular structure that is not as well captured by clustering or other dimensionality reduction methods. However, interpreting the individual parts remains a challenge. To address this challenge, we extend methods for differential expression analysis by allowing cells to have partial membership to multiple groups. We call this grade of membership differential expression (GoM DE). We illustrate the benefits of GoM DE for annotating topics identified in several single-cell RNA-seq and ATAC-seq data sets.
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- 2023
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25. Identification and experimental validation of key extracellular proteins as potential targets in intervertebral disc degeneration
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Guang-Zhi Zhang, Lei Li, Zhang-Bin Luo, Cang-Yu Zhang, Yong-Gang Wang, and Xue-Wen Kang
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intervertebral disc degeneration ,extracellular proteins ,differentially expressed genes ,protein-protein interactions ,bioinformatics analysis ,collagen ,gene expression ,micrornas (mirnas) ,cytoscape ,gene expression profiling ,t-test ,nucleus pulposus tissues ,nucleus pulposus ,intervertebral disc (ivd) ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Aims: This study aimed, through bioinformatics analysis and in vitro experiment validation, to identify the key extracellular proteins of intervertebral disc degeneration (IDD). Methods: The gene expression profile of GSE23130 was downloaded from the Gene Expression Omnibus (GEO) database. Extracellular protein-differentially expressed genes (EP-DEGs) were screened by protein annotation databases, and we used Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) to analyze the functions and pathways of EP-DEGs. STRING and Cytoscape were used to construct protein-protein interaction (PPI) networks and identify hub EP-DEGs. NetworkAnalyst was used to analyze transcription factors (TFs) and microRNAs (miRNAs) that regulate hub EP-DEGs. A search of the Drug Signatures Database (DSigDB) for hub EP-DEGs revealed multiple drug molecules and drug-target interactions. Results: A total of 56 EP-DEGs were identified in the differential expression analysis. EP-DEGs were enriched in the extracellular structure organization, ageing, collagen-activated signalling pathway, PI3K-Akt signalling pathway, and AGE-RAGE signalling pathway. PPI network analysis showed that the top ten hub EP-DEGs are closely related to IDD. Correlation analysis also demonstrated a significant correlation between the ten hub EP-DEGs (p<0.05), which were selected to construct TF–gene interaction and TF–miRNA coregulatory networks. In addition, ten candidate drugs were screened for the treatment of IDD. Conclusion: The findings clarify the roles of extracellular proteins in IDD and highlight their potential as promising novel therapeutic targets. Cite this article: Bone Joint Res 2023;12(9):522–535.
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- 2023
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26. GhCKX14 responding to drought stress by modulating antioxi-dative enzyme activity in Gossypium hirsutum compared to CKX family genes
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Tengyu Li, Kun Luo, Chenlei Wang, Lanxin Wu, Jingwen Pan, Mingyang Wang, Jinwei Liu, Yan Li, Jinbo Yao, Wei Chen, Shouhong Zhu, and Yongshan Zhang
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Cytokinin oxidase/dehydrogenase ,Antioxidant ,Abiotic stress ,Gene expression ,Hormones ,Botany ,QK1-989 - Abstract
Abstract Background Cytokinin oxidase/dehydrogenase (CKX) plays a vital role in response to abiotic stress through modulating the antioxidant enzyme activities. Nevertheless, the biological function of the CKX gene family has yet to be reported in cotton. Result In this study, a total of 27 GhCKXs were identified by the genome-wide investigation and distributed across 18 chromosomes. Phylogenetic tree analysis revealed that CKX genes were clustered into four clades, and most gene expansions originated from segmental duplications. The CKXs gene structure and motif analysis displayed remarkably well conserved among the four groups. Moreover, the cis-acting elements related to the abiotic stress, hormones, and light response were identified within the promoter regions of GhCKXs. Transcriptome data and RT-qPCR showed that GhCKX genes demonstrated higher expression levels in various tissues and were involved in cotton’s abiotic stress and phytohormone response. The protein-protein interaction network indicates that the CKX family probably participated in redox regulation, including oxidoreduction or ATP levels, to mediate plant growth and development. Functionally identified via virus-induced gene silencing (VIGS) found that the GhCKX14 gene improved drought resistance by modulating the antioxidant-related activitie. Conclusions In this study, the CKX gene family members were analyzed by bioinformatics, and validates the response of GhCKX gene to various phytohormone treatment and abiotic stresses. Our findings established the foundation of GhCKXs in responding to abiotic stress and GhCKX14 in regulating drought resistance in cotton.
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- 2023
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27. Molecular Cloning and Characterization of Scavenger Receptor Class B Type 1 in Grass Carp (Ctenopharyngodon idellus) and Its Expression Profile following Grass Carp Reovirus Challenge
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Yang Zhang, Jiayuan Shi, Yuntao Lu, Qing Luo, Pengfei Chu, Rong Huang, Kunci Chen, Jian Zhao, Yaping Wang, and Mi Ou
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grass carp ,GCRV ,SRB1 ,gene expression ,age-dependent susceptibility ,Biology (General) ,QH301-705.5 ,Genetics ,QH426-470 - Abstract
As a member of the pattern recognition receptor (PRR) class, scavenger receptor class B type 1 (SRB1) plays a key role in innate immunity. Grass carp (Ctenopharyngodon idellus) ranks among the most extensively cultivated freshwater aquaculture species in China. However, little is known about the function of SRB1 in C. idellus. In this research study, a SRB1 gene was identified in C. idellus, named CiSRB1. The full-length cDNA of CiSRB1 is 2486 bp long, with an open reading frame (ORF) of 2486 bp encoding a 497 amino acid (aa) protein containing a conserved CD36 domain. The identified genomic DNA length of CiSRB1 is 20,042 bp, including 12 exons and 11 introns. The predictive analysis of protein interactions revealed that CiSRB1 could interact with the outer capsid proteins of typical GCRV strains. The tissue distribution of CiSRB1 exhibited age-dependent characteristics. CiSRB1 displayed the highest expression in the intestines and moderate levels in muscle, spleen, liver, and brain of one-year-old grass carp while maintaining relatively low levels in three-year-old grass carp. Following grass carp reovirus (GCRV) infection, notable upregulation of CiSRB1 transcripts was observed in major immune tissues (gills, intestines, spleen, and liver). Furthermore, significant differences were found between one-year-old and three-year-old grass carp, with lower CiSRB1 expression levels being detected in the older group. Additionally, a distinct response to GCRV infection was observed in one-year-old and three-year-old grass carp. It was found that one-year-old individuals had a mortality rate of up to 84% 6 days post-infection (dpi), whereas all three-year-old counterparts survived after GCRV infection. The analysis of GCRV copy numbers across tissues revealed substantially higher levels in one-year-old grass carp compared with their older counterparts, confirming the existence of age-dependent susceptibility to GCRV infection in grass carp. Combined with these results, it was speculated that the decline in cell-surface CiSRB1 expression with age may impede reovirus binding to host cells, potentially explaining why older grass carp demonstrated enhanced resistance to GCRV infection. This observation accentuates the importance of CiSRB1 in the context of GCRV infection and provides insights into age-dependent susceptibility to reovirus.
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- 2024
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28. Genome-wide identification of the bHLH transcription factor family in Rosa persica and response to low-temperature stress
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Yueying Zhuang, Lijun Zhou, Lifang Geng, Lv Jiang, Yunji Sui, Le Luo, Huitang Pan, Qixiang Zhang, and Chao Yu
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Rosa persica ,Basic helix-loop-helix transcription factor ,Gene expression ,Transcription factor ,Low-temperature stress ,Medicine ,Biology (General) ,QH301-705.5 - Abstract
Background Basic helix-loop-helix (bHLH) transcription factors are involved in plant growth and development, secondary metabolism, and abiotic stress responses have been studied in a variety of plants. Despite their importance in plant biology, the roles and expression patterns of bHLH family genes in Rosa persica have not been determined. Methods In this study, the RbebHLH family genes were systematically analyzed using bioinformatics methods, and their expression patterns under low-temperature stress were analyzed by transcriptome and related physiological index measurements. Results In total, 142 RbebHLHs were identified in the genome of R. persica, distributed on seven chromosomes. Phylogenetic analysis including orthologous genes in Arabidopsis divided RbebHLHs into 21 subfamilies, with similar structures and motifs within a subfamily. A collinearity analysis revealed seven tandem duplications and 118 segmental duplications in R. persica and 127, 150, 151, 172, and 164 segmental duplications between R. persica and Arabidopsis thaliana, Prunus mume, Fragaria vesca, Rosa chinensis, and Prunus persica, respectively. A number of cis-regulatory elements associated with abiotic stress response and hormone response were identified in RbebHLHs, and 21 RbebHLHs have potential interactions with the CBF family. In addition, the expression results showed that part of bHLH may regulate the tolerance of R. persica to low-temperature stress through the jasmonic acid and pathway. Transcriptomic data showed that the expression levels of different RbebHLHs varied during overwintering, and the expression of some RbebHLHs was significantly correlated with relative conductivity and MDA content, implying that RbebHLHs play important regulatory roles in R. persica response to low-temperature stress. Overall, this study provides valuable insights into the study of RbebHLHs associated with low-temperature stress.
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- 2024
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29. Nonadditive and allele-specific expression of ghrelin in hybrid tilapia
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Huan Zhong, Bingxin Ren, Chenyi Lou, Yi Zhou, Yongju Luo, and Jun Xiao
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ghrelin ,hybrid tilapia ,nonadditive expression ,gene expression ,heterosis ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
BackgroundInterspecies hybridization is an important breeding method to generate fishes with heterosis in aquaculture. Using this method, hybrid Nile tilapia (Oreochromis niloticus, ♀) × blue tilapia (Oreochromis aureus, ♂) has been produced and widely farmed due to its growth and appetite superiorities. However, the genetic mechanism of these advanced traits is still not well understood. Ghrelin is a crucial gene that regulates growth and appetite in fishes. In the present study, we focused on the expression characteristics and its regulation of ghrelin in the hybrid.ResultsThe tissue distribution analysis showed that ghrelin was predominantly expressed in the stomach in the hybrid. Ghrelin was more highly expressed in the stomach in the hybrid and Nile tilapia, compared to blue tilapia, showing a nonadditive pattern. Two single-nucleotide polymorphism (SNP) sites were identified including T/C and C/G from the second exon in the ghrelin gene from Nile tilapia and blue tilapia. By pyrosequencing based on the SNP sites, the allele-specific expression (ASE) of ghrelin in the hybrid was assayed. The result indicated that ghrelin in the hybrid showed higher maternal allelic transcript ratios. Fasting significantly increased ghrelin overall expression at 4, 8, 12, 24, and 48 h. In addition, higher maternal allelic transcript ratios were not changed in the fasting hybrids at 48 h. The cis and trans effects were determined by evaluating the overall expression and ASE values in the hybrid. The expression of ghrelin was mediated by compensating cis and trans effects in hybrid.ConclusionIn summary, the present lines of evidence showed the nonadditive expression of ghrelin in the hybrid tilapia and its regulation by subgenomes, offering new insight into gene expression characteristics in hybrids.
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- 2023
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30. Overexpression of soybean GmDHN9 gene enhances drought resistance of transgenic Arabidopsis
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Jiayi Fan, Yuzhe Zhang, Hongji Sun, Ruijie Duan, Yushi Jiang, Xinyu Wang, Yao Sun, Zhipeng Luo, Peiwu Wang, Shuyan Guan, Siyan Liu, Xuhong Fan, Peng Jiao, Yunpeng Wang, Jinhui Yang, Zunyue Zhang, and Huiwei Yu
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dehydrin ,drought resistance ,function ,gene expression ,reactive oxygen species ,Plant culture ,SB1-1110 ,Genetics ,QH426-470 - Abstract
ABSTRACTSoybean is one of the important oil crops and a major source of protein and lipids. Drought can cause severe soybean yields. Dehydrin protein (DHN) is a subfamily of LEA proteins that play an important role in plant responses to abiotic stresses. In this study, the soybean GmDHN9 gene was cloned and induced under a variety of abiotic stresses. Results showed that the GmDHN9 gene response was more pronounced under drought induction. Subcellular localization results indicated that the protein was localized in the cytoplasm. The role of transgenic Arabidopsis plants in drought stress response was further studied. Under drought stress, the germination rate, root length, chlorophyll, proline, relative water content, and antioxidant enzyme content of transgenic Arabidopsis thaliana transgenic genes were higher than those of wild-type plants, and transgenic plants contained less O2−, H2O2 and MDA contents. In short, the GmDHN9 gene can regulate the homeostasis of ROS and enhance the drought resistance of plants.
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- 2024
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31. RNAi-mediated knockdown of the mandibular organ-inhibiting hormone (MOIH) gene stimulates vitellogenesis in the Chinese mitten crab Eriocheir sinensis
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Ding, Shuquan, Huang, Mengting, Sheng, Na, Chen, Tiantian, Xu, Ruihan, Luo, Zhaoyuan, Huang, Xiuqin, Wan, Zhicheng, Su, Shiping, and Li, Xilei
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- 2023
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32. White Isthmus Transcriptome Analysis Reveals the Mechanism of Translucent Eggshell Formation
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Ying Ma, Yuxing Luo, Wen Li, Dehe Wang, and Zhonghua Ning
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translucent eggs ,transcriptome ,gene expression ,laying hens ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
The presence of translucent eggshells is a type of egg quality issue that impacts egg sales. While many researchers have studied them, the exact mechanisms behind their formation remain unclear. In this study, we conducted a transcriptomic differential expression analysis of the isthmus region of the oviduct in both normal egg- and translucent egg-laying hens. The analysis revealed that differentially expressed gene pathways were predominantly concentrated in the synthesis, modification, and transport of eggshell membrane proteins, particularly collagen proteins, which provide structural support. These findings suggest that variations in the physical structure of the eggshell membrane, resulting from changes in its chemical composition, are the fundamental cause of translucent eggshell formation. This research provides a theoretical reference for reducing the occurrence of translucent eggs.
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- 2024
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33. Molecular Characterization and Expression Analysis of the C-Type Lectin Domain Family 4 Member F in Litopenaeus vannamei against White Spot Syndrome Virus
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Qian Xue, Bingbing Yang, Kun Luo, Sheng Luan, Jie Kong, Xupeng Li, and Xianhong Meng
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viral disease control ,innate immunity ,LvCLEC4F ,WSSV ,gene expression ,RNAi ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
White spot disease (WSD) outbreaks pose a significant threat to the Pacific white shrimp (Litopenaeus vannamei) farming industry. The causative agent is the white spot syndrome virus (WSSV). There are no effective treatments for WSD so far. Therefore, understanding the resistance mechanisms of L. vannamei against the WSSV is crucial. C-type lectins (CTLs) are important pattern recognition receptors (PRRs) that promote agglutination, phagocytosis, encapsulation, bacteriostasis, and antiviral infections. This study cloned the C-type lectin domain family 4 member F (LvCLEC4F) from L. vannamei. LvCLEC4F contains a 492 bp open reading frame (ORF) encoding a protein of 163 amino acids, including a carbohydrate recognition domain (CRD). Following a challenge with the WSSV, the expression profile of LvCLEC4F was significantly altered. Using RNA interference (RNAi) technology, it was found that LvCLEC4F promotes WSSV replication and affects the expression levels of genes related to the regulation of apoptosis, signaling and cellular stress response, and immune defense. Meanwhile, the hemolymph agglutination phenomenon in vivo was weakened when LvCLEC4F was knocked down. These results indicated that LvCLEC4F may play an important role in the interaction between L. vannamei and WSSV.
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- 2024
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34. Comparative transcriptome analysis of Ts (Resistant genotype) and Ma (Susceptible genotype) marigold (Tagetes erecta L.) leaves in response to Alternaria tagetica
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Xi Cheng, Dongliang Chen, Chang Luo, Hua Liu, and Conglin Huang
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Tagetes erecta ,Co-expression network ,Gene expression ,Black spot resistance ,Transcriptional modules ,Plant culture ,SB1-1110 - Abstract
Marigold black spot caused by Alternaria tagetica is a major disease that can decrease marigold production by 40%, resulting in serious economic losses. In this study, we identified many genes responsive to A. tagetica in the resistant and susceptible marigold genotypes. Analyses of differentially expressed genes, expression trends, and a weighted gene co-expression network revealed a series of hub genes with key roles in different A. tagetica infection stages. Additionally, 1 216 unigenes encoding transcription factors from eight families were differentially expressed between Ts and Ma. Moreover, R genes from various families (e.g., N, NL, RLP, and TNL) were differentially expressed in the two marigold genotypes before and after the inoculation with A. tagetica. Pathway diagrams were used to visualize the leaf transcriptional changes in the two marigold genotypes infected by A. tagetica to clarify the effects of A. tagetica on the expression patterns of genes involved in phosphatidylinositol signaling, plant–pathogen interactions, and plant hormone signal transduction. We identified candidate genes related to disease resistance and generated valuable resources for analyzing the candidate gene functions related to black spot resistance in marigold. The study data may be useful for the molecular marker-assisted screening and breeding of marigold lines with increased disease resistance.
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- 2023
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35. Uncovering expression and functional analysis of newly discovered high-affinity K+ transporter family members from sugarcane
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Luo, Hai-bin, Huang, Cheng-mei, Wei, Yuan-wen, Zhu, Hui-ming, Cao, Hui-qing, Jiang, Sheng-li, Deng, Zhi-nian, Wu, Kai-chao, and Xu, Lin
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- 2022
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36. Functional differentiation of the ovine preadipocytes —insights from gene expression profiling
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Hao, Zhiyun, Jin, Xiayang, Wang, Jiqing, Luo, Yuzhu, Hu, Jiang, Liu, Xiu, Li, Shaobin, Zhao, Fangfang, and Li, Mingna
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- 2023
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37. Varying the expression pattern of the strigolactone receptor gene DAD2 results in phenotypes distinct from both wild type and knockout mutants
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Revel S. M. Drummond, Hui Wen Lee, Zhiwei Luo, Jack F. Dakin, Bart J. Janssen, and Kimberley C. Snowden
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strigolactone ,branch ,development ,gene expression ,mutation ,receptor ,Plant culture ,SB1-1110 - Abstract
The action of the petunia strigolactone (SL) hormone receptor DAD2 is dependent not only on its interaction with the PhMAX2A and PhD53A proteins, but also on its expression patterns within the plant. Previously, in a yeast-2-hybrid system, we showed that a series of a single and double amino acid mutants of DAD2 had altered interactions with these binding partners. In this study, we tested the mutants in two plant systems, Arabidopsis and petunia. Testing in Arabidopsis was enabled by creating a CRISPR-Cas9 knockout mutant of the Arabidopsis strigolactone receptor (AtD14). We produced SL receptor activity in both systems using wild type and mutant genes; however, the mutants had functions largely indistinguishable from those of the wild type. The expression of the wild type DAD2 from the CaMV 35S promoter in dad2 petunia produced plants neither quite like the dad2 mutant nor the V26 wild type. These plants had greater height and leaf size although branch number and the plant shape remained more like those of the mutant. These traits may be valuable in the context of a restricted area growing system such as controlled environment agriculture.
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- 2023
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38. Effect of sublethal dose of chloramphenicol on biofilm formation and virulence in Vibrio parahaemolyticus
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Miaomiao Zhang, Liyan Cai, Xi Luo, Xue Li, Tingting Zhang, Fei Wu, Yiquan Zhang, and Renfei Lu
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Vibrio parahaemolyticus ,chloramphenicol ,gene expression ,biofilm ,virulence ,Microbiology ,QR1-502 - Abstract
Vibrio parahaemolyticus isolates are generally very sensitive to chloramphenicol. However, it is usually necessary to transfer a plasmid carrying a chloramphenicol resistance gene into V. parahaemolyticus to investigate the function of a specific gene, and the effects of chloramphenicol on bacterial physiology have not been investigated. In this work, the effects of sublethal dose of chloramphenicol on V. parahaemolyticus were investigated by combined utilization of various phenotypic assays and RNA sequencing (RNA-seq). The results showed that the growth rate, biofilm formation capcity, c-di-GMP synthesis, motility, cytoxicity and adherence activity of V. parahaemolyticus were remarkably downregulated by the sublethal dose of chloramphenicol. The RNA-seq data revealed that the expression levels of 650 genes were significantly differentially expressed in the response to chloramphenicol stress, including antibiotic resistance genes, major virulence genes, biofilm-associated genes and putative regulatory genes. Majority of genes involved in the synthesis of polar flagellum, exopolysaccharide (EPS), mannose-sensitive haemagglutinin type IV pilus (MSHA), type III secretion systems (T3SS1 and T3SS2) and type VI secretion system 2 (T6SS2) were downregulated by the sublethal dose of chloramphenicol. Five putative c-di-GMP metabolism genes were significantly differentially expressed, which may be the reason for the decrease in intracellular c-di-GMP levels in the response of chloramphenicol stress. In addition, 23 genes encoding putative regulators were also significantly differentially expressed, suggesting that these regulators may be involved in the resistance of V. parahaemolyticus to chloramphenicol stress. This work helps us to understand how chloramphenicol effect on the physiology of V. parahaemolyticus.
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- 2023
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39. SPACA6P-AS: a trailblazer in breast cancer pathobiology and therapeutics
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Feng, Wenjie, Jiang, Yiling, Zeng, Lijun, Ouyang, Yuhan, Li, Hailong, Tang, Yuanbin, Luo, Lunqi, Ouyang, Lianjie, Xie, Liming, Tan, Yeru, and Li, Yuehua
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- 2024
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40. Isolation and expression analysis of cellulose synthase 3 (Ces3) genes from sugarcane (Saccharum officinarum L.)
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Huang, Cheng-Mei, Wu, Kai-Chao, Verma, Krishan K., Luo, Hai-Bin, Deng, Zhi-Nian, Shi, Guo-Ying, Cao, Hui-Qing, Wu, Xing-Jian, Ye, Li-Ping, Wei, Yuan-Wen, and Xu, Lin
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- 2024
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41. Identification of NINJ1 as a novel prognostic predictor for retroperitoneal liposarcoma
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Zhao, Yu, Qin, Da, Li, Xiangji, Wang, Tiange, Zhang, Tong, Rao, Xiaosong, Min, Li, Wan, Zhiyi, Luo, Chenghua, and Xiao, Mengmeng
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- 2024
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42. Circadian rhythm response and its effect on photosynthetic characteristics of the Lhcb family genes in tea plant
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Hu, Zhi-Hang, Zhang, Nan, Qin, Zhi-Yuan, Li, Jing-Wen, Tao, Jian-Ping, Yang, Ni, Chen, Yi, Kong, Jie-Yu, Luo, Wei, Chen, Xuan, Li, Xing-Hui, Xiong, Ai-Sheng, and Zhuang, Jing
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- 2024
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43. Systematic characterization of Gossypium GLN family genes reveals a potential function of GhGLN1.1a regulates nitrogen use efficiency in cotton
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Li, Xiaotong, Gu, Yunqi, Kayoumu, Mirezhatijiang, Muhammad, Noor, Wang, Xiangru, Gui, Huiping, Luo, Tong, Wang, Qianqian, Wumaierjiang, Xieraili, Ruan, Sijia, Iqbal, Asif, Zhang, Xiling, Song, Meizhen, and Dong, Qiang
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- 2024
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44. Whole genome identification, molecular docking and expression analysis of enzymes involved in the selenomethionine cycle in Cardamine hupingshanensis
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Zeng, Xixi, Luo, Guoqiang, Fan, Zhucheng, Xiao, Zhijing, Lu, Yanke, Xiao, Qiang, Hou, Zhi, Tang, Qiaoyu, and Zhou, Yifeng
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- 2024
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45. Integrated metabolome and transcriptome analyses reveal the molecular mechanism underlying dynamic metabolic processes during taproot development of Panax notoginseng
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Li, Xuejiao, Zhao, Yan, He, Shuilian, Meng, Jing, Lu, Yingchun, Shi, Huineng, Liu, Chunlan, Hao, Bing, Tang, Qingyan, Zhang, Shuangyan, Zhang, Guanghui, Luo, Yu, Yang, Shengchao, Yang, Jianli, and Fan, Wei
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- 2024
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46. Functional characterization of D-type cyclins involved in cell division in rice
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Jiang, Zhishu, Wang, Xin, Zhou, Zhiwei, Peng, Limei, Lin, Xiaoli, Luo, Xiaowei, Song, Yongping, Ning, Huaying, Gan, Cong, He, Xiaopeng, Zhu, Changlan, Ouyang, Linjuan, Zhou, Dahu, Cai, Yicong, Xu, Jie, He, Haohua, and Liu, Yantong
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- 2024
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47. PbrWRKY62-PbrADC1 module involves in superficial scald development of Pyrus bretschneideri Rehd.fruit via regulating putrescine biosynthesis
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Zhang, Xu, Zhu, Lijuan, Qian, Ming, Jiang, Li, Gu, Peng, Jia, Luting, Qian, Chunlu, Luo, Weiqi, Ma, Min, Wu, Zhangfei, Qiao, Xin, Wang, Libin, and Zhang, Shaoling
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- 2024
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48. Effects of paclobutrazol seed priming on seedling quality, photosynthesis, and physiological characteristics of fragrant rice
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Zhang, Yingying, He, Zhenzhen, Xing, Pipeng, Luo, Haowen, Yan, Zhuosheng, and Tang, Xiangru
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- 2024
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49. Effects of Different Light Qualities on Color Development and Anthocyanin Metabolism of Peach Skin during Postharvest Storage
- Author
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CHI Ming, SUN Lijuan, MA Lijie, ZHAO Jing, ZHOU Hongsheng, LING Jun, LUO Shufen, LI Guofeng, LI Pengxia, ZHANG Yingtong
- Subjects
peach ,light ,color ,anthocyanin ,gene expression ,Food processing and manufacture ,TP368-456 - Abstract
The objective of this work was to study the effects of different light treatments on the color development of peach skin during postharvest storage and to explore the regulatory mechanism of light on anthocyanin metabolism. Peach fruits, cv. Zhongtao 9, were subjected to different lights (red, green, blue and white) for 12 h and darkness for another 12 h at (22 ± 1) ℃, while those kept in darkness for 24 h were considered as a control. The variations in fruit skin color, anthocyanin content and enzymatic activities involved in the anthocyanin metabolic pathway, as well as the expression levels of the structural genes of anthocyanin biosynthesis and related transcription factors after light treatment were measured. The results showed that the color development of peach skin was not affected by red and green light, but was weakly promoted by white light and significantly induced by blue light. The anthocyanin content in blue light-treated peach skin was 27.26 mg/kg, which was 4.48 and 10.34 times as high as that in the white light treatment and control groups on the sixth day after harvest, respectively. Under blue light treatment, the enzymatic activities of the anthocyanin biosynthesis pathway and the expression levels of structural genes and transcription factors were significantly higher than those in the control and other light treatment groups at most of the time points tested (P < 0.05). The expression levels of the structural genes PAL, CHS, F3H, DFR, ANS and UFGT and the transcription factor MYB10.1 showed significantly positive correlation with the color parameters and anthocyanin content of peach skin. Collectively, this study indicated that blue light treatment promoted the synthesis and accumulation of anthocyanins in peach skin by up-regulating the anthocyanin synthesis pathway, which will deepen the understanding of the regulatory mechanism of anthocyanin metabolism by light in plants and provide a theoretical basis for the development of postharvest technologies to improve peach skin color.
- Published
- 2023
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50. Trans-Zeatin induced regulation of the biosynthesis of 2-acetyl-1-pyrroline in fragrant rice (Oryza sativa L.) seedlings
- Author
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Pipeng Xing, Haowen Luo, Zhenzhen He, Longxin He, Hua Zhao, Xiangru Tang, and Meiyang Duan
- Subjects
Gene expression ,Trans-zeatin ,Fragrant rice ,2-acetyl-1-pyrroline ,Proline ,Botany ,QK1-989 - Abstract
Abstract Background In plants, cytokinin is activated into trans-zeatin to fight abiotic stresses. However, the mechanism of the effect of trans-zeatin on 2-acetyl-1-pyrroline (2-AP) biosynthesis in fragrant rice has yet to be studied. The present study was conducted to explore the effects of exogenous trans-zeatin on enzymes activities, genes expression, and precursors involved in 2-AP biosynthesis and 2-AP contents as well as the seedling quality of a fragrant rice cultivar viz., Meixiangzhan2. Four concentrations of trans-zeatin solutions at 20, 40, and 80 μmol L− 1 (ZT1, ZT2, and ZT3) were sprayed onto rice seedlings. Results Compared to the control, trans-zeatin treatments showed significantly higher 2-AP contents of fragrant rice seedlings. Increased plant height and stem width were observed due to trans-zeatin treatments. The trans-zeatin application increased 1-pyrroline, methylglyoxal, proline, and P5C contents, enhanced P5CS and OAT activities, and reduced glutamic acid contents. In addition, expressions of ProDH, P5CS2, and DAO4 were comparatively higher under trans-zeatin treatments than CK in fragrant rice seedlings. Conclusions Overall, up-regulation of P5C, 1-pyrroline, and proline and down-regulation of glutamic acid under appropriate trans-zeatin concentrations (20 and 40 μmol L− 1) resulted in enhanced 2-AP biosynthesis in fragrant rice seedlings and 20–40 μmol L− 1 was considered as the suggested concentrations of trans-zeatin application in fragrant rice seedling.
- Published
- 2023
- Full Text
- View/download PDF
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