1. Effect of ceftriaxone on paired-pulse response and long-term potentiation of hippocampal dentate gyrus neurons in rats with Alzheimer-like disease.
- Author
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Hamidi N, Nozad A, Sheikhkanloui Milan H, Salari AA, and Amani M
- Subjects
- Alzheimer Disease chemically induced, Alzheimer Disease pathology, Animals, Anti-Bacterial Agents pharmacology, Carcinogens toxicity, Dentate Gyrus pathology, Hippocampus pathology, Male, Neuronal Plasticity drug effects, Neurons pathology, Okadaic Acid toxicity, Rats, Rats, Wistar, Synapses drug effects, Alzheimer Disease drug therapy, Ceftriaxone pharmacology, Dentate Gyrus drug effects, Excitatory Postsynaptic Potentials drug effects, Hippocampus drug effects, Long-Term Potentiation drug effects, Neurons drug effects
- Abstract
Aims: Glutamatergic dysfunction is posed as a main stage in neurodegenerative disorders such as Alzheimer's disease (AD). Glutamate-mediated excitotoxicity contributes to cognitive dysfunction and cell death in AD. Ceftriaxone (CFT), a well-known upregulator of GLT-1, selectively induces the expression of glutamate transporter-1 (GLT-1) in different brain regions and therefore can be posed as a potential candidate for elimination of glutamate-induced excitotoxicity which is an early prominent event in AD brains. This study was designed to investigate the electrophysiological and behavioral effects of the β-lactam antibiotic ceftriaxone in okadaic acid (OKA)-induced model of AD., Materials and Methods: Male Wistar rats divided into four control, ceftriaxone (CFT), OKA, and OKA plus ceftriaxone (OKA + CFT) groups. OKA was injected intracerebroventricularly (i.c.v., 200 ng/5 μl) into lateral ventricles and after two weeks the evoked field potential recorded from hippocampal perforant path-DG synapses in order to evaluate the effect of ceftriaxone treatment (200 mg/kg/day, i.p.) on long-term potentiation (LTP) and paired-pulse responses., Key Findings: Results of this study revealed that ceftriaxone treatment significantly ameliorates the OKA-induced attenuation of field excitatory post-synaptic potential (fEPSP) slope and population spike (PS) amplitude following high-frequency stimulation and paired-pulse paradigm indicating its beneficial effects on both short-term and long-term plasticity in these neurons. Ceftriaxone also has an improving effect on OKA-induced impairment in short- and long-term memories evaluated by alternation behavior and passive avoidance tasks in rats., Significance: Therefore, this study suggests that GLT-1 might be a promising therapeutic target for treatment of neurodegenerative disorders such as AD in the future., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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