1. T cell activation threshold regulated by E3 ubiquitin ligase Cbl-b determines fate of inducible regulatory T cells.
- Author
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Guilin Qiao, Yixia Zhao, Zhenping Li, Tang, Peter Q., Langdon, Wallace Y., Tianlan Yang, and Jian Zhang
- Subjects
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T cells , *UBIQUITIN ligases , *T cell receptors , *T cell differentiation , *MTOR protein , *GENE expression in mammals , *PROTEIN kinase B , *PHYSIOLOGY - Abstract
E3 ubiquitin ligase Casitas-B-lineage lymphoma protein-b (Cbl-b) is critical for establishing the threshold for T cell activation and is essential for induction of T cell anergy. Recent studies suggest that Cbl-b is involved in the development of CD4+CD25+ inducible regulatory T cells (iTregs). In this study, we report that the optimal induction of Foxp3 by naive CD4+CD25- T cells requires suboptimal TCR triggering. In the absence of Cbl-b, the TCR strength for optimal Foxp3 induction is downregulated in vitro. Using TCR-transgenic Rag-/- mice in combination with Cbl-b deficiency, we show that in vivo iTreg development is also controlled by Cbl-b via tuning the TCR strength. Furthermore, we show that Akt-2 but not Akt-1 regulates Foxp3 expression downstream of Cbl-b. Therefore, we demonstrate that Cbl-b regulates the fate of iTregs via controlling the threshold for T cell activation. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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