5 results on '"Kumar, Sundeep"'
Search Results
2. Genomic characterization of drought tolerance-related traits in spring wheat.
- Author
-
Kumar, Sundeep, Sehgal, Sunish, Kumar, Uttam, Prasad, P., Joshi, Arun, and Gill, Bikram
- Subjects
- *
WHEAT , *CROPS , *DROUGHT tolerance , *PLANT gene mapping , *MICROSATELLITE repeats in plants , *PLANT molecular genetics - Abstract
Drought tolerance was investigated in 'C306', one of the most drought tolerant wheat cultivars bred in India in the 1960's. An intervarietal mapping population of recombinant inbred lines of the cross 'C306' × 'HUW206' was evaluated for drought tolerance components, namely potential quantum efficiency of photosystem (PS) II (F/F), chlorophyll content (Chl), flag leaf temperature (Lt), and grain yield per plant (Gyp) under stress. Three independent experiments were conducted under well-watered and water-stressed conditions in greenhouses and growth chambers at Kansas State University (USA). Five hundred and sixty microsatellite markers covering the entire genome were screened for polymorphism between the parents. A QTL ( QLt.ksu- 1D) for Lt (low flag leaf temperature under stress) on the short arm of chromosome 1D between markers Xbarc271 and Xgwm337 at LOD 3.5 explained 37% of the phenotypic variation. A QTL for F/F ( QF /F .ksu- 3B) and Chl ( QChl.ksu- 3B) controlling quantum efficiency of PS II and chlorophyll content under stress were co-localized on chromosome 3B in the marker interval Xbarc68-X barc101 and explained 35-40% of the phenotypic variation for each trait. A QTL ( QGyp.ksu- 4A) for Gyp on chromosome 4A at a LOD value of 3.2 explained 16.3% of the phenotypic variation. Inconsistent QTLs were observed for F/F on chromosomes 3A, 6A, 2B, 4B, and 4D; for Chl on 3A, 6A, 2B and 4B; and for Lt on 1A, 3A 6A, 3B and 5B. The identified QTLs give a first glimpse of the genetics of drought tolerance in C306 and need to be validated in field experiments using the marker-phenotype linkages reported here. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
3. TinoTranscriptDB: A Database of Transcripts and Microsatellite Markers of Tinospora cordifolia , an Important Medicinal Plant.
- Author
-
Singh, Rakesh, Mahato, Ajay Kumar, Singh, Akshay, Kumar, Rajesh, Singh, Amit K., Kumar, Sundeep, Marla, Soma S., Kumar, Ashok, and Singh, Nagendra K.
- Subjects
- *
TINOSPORA cordifolia , *MICROSATELLITE repeats , *MEDICINAL plants , *SHORT tandem repeat analysis , *SPECIFIC gravity , *TRANSCRIPTION factors , *TANDEM repeats - Abstract
Tinospora cordifolia, commonly known as "Giloe" in India, is a shrub belonging to the family Menispermaceae. It is an important medicinal plant known for its antipyretic, anti-inflammatory, antispasmodic, and antidiabetic properties and is used in the treatment of jaundice, gout, and rheumatism. Despite its economic importance, the limited information related to its genomic resources prohibits its judicious exploitation through molecular breeding or biotechnological approaches. In this study, we generated a meta-transcriptome assembly of 43,090 non-redundant transcripts by merging the RNASeq data obtained from Roche 454 GS-FLX, and Illumina platforms, and report the first transcriptome-based database for simple sequence repeats and transcription factors ("TinoTranscriptDB" (Tinospora cordifolia Transcriptome Database)). We annotated 26,716 (62%) of the total transcripts successfully from National Center for Biotechnology Information non-redundant protein (NCBI-NR), gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Swiss-Prot, and Pfam databases. This database contains information of 2620 perfect simple sequence repeats (P-SSRs) with a relative abundance of 340.12 (loci/Mb), and relative density of 6309.29 (bp/Mb). Excluding mono-nucleotides, the most abundant SSR motifs were tri-nucleotides (54.31%), followed by di-nucleotides (37.51%), tetra-nucleotides (4.54%), penta-nucleotides (3.16%) and hexa-nucleotides (0.45%). Additionally, we also identified 4,311 transcription factors (TFs) and categorized them into 55 sub-families. This database is expected to fill the gap in genomic resource availability in T. cordifolia and thus accelerate molecular breeding and related functional and other applied studies aimed towards genetic improvements of T. cordifolia and related species. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
4. Genetic diversity trend in Indian rice varieties: an analysis using SSR markers.
- Author
-
Singh, Nivedita, Choudhury, Debjani Roy, Tiwari, Gunjan, Singh, Amit Kumar, Kumar, Sundeep, Srinivasan, Kalyani, Tyagi, R. K., Sharma, A. D., Singh, N. K., and Singh, Rakesh
- Subjects
- *
RICE varieties , *PLANTS , *RICE , *BIOMARKERS , *RICE breeding ,RICE genetics - Abstract
Background: The knowledge of the extent and pattern of diversity in the crop species is a prerequisite for any crop improvement as it helps breeders in deciding suitable breeding strategies for their future improvement. Rice is the main staple crop in India with the large number of varieties released every year. Studies based on the small set of rice genotypes have reported a loss in genetic diversity especially after green revolution. However, a detailed study of the trend of diversity in Indian rice varieties is lacking. SSR markers have proven to be a marker of choice for studying the genetic diversity. Therefore, the present study was undertaken with the aim to characterize and assess trends of genetic diversity in a large set of Indian rice varieties (released between 1940-2013), conserved in the National Gene Bank of India using SSR markers. Result: A set of 729 Indian rice varieties were genotyped using 36 HvSSR markers to assess the genetic diversity and genetic relationship. A total of 112 alleles was amplified with an average of 3.11 alleles per locus with mean Polymorphic Information Content (PIC) value of 0.29. Cluster analysis grouped these varieties into two clusters whereas the model based population structure divided them into three populations. AMOVA study based on hierarchical cluster and model based approach showed 3% and 11% variation between the populations, respectively. Decadal analysis for gene diversity and PIC showed increasing trend from 1940 to 2005, thereafter values for both the parameters showed decreasing trend between years 2006-2013. In contrast to this, allele number demonstrated increasing trend in these varieties released and notified between1940 to 1985, it remained nearly constant during 1986 to 2005 and again showed an increasing trend. Conclusion: Our results demonstrated that the Indian rice varieties harbors huge amount of genetic diversity. However, the trait based improvement program in the last decades forced breeders to rely on few parents, which resulted in loss of gene diversity during 2006 to 2013. The present study indicates the need for broadening the genetic base of Indian rice varieties through the use of diverse parents in the current breeding program. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
5. Molecular diversity and SSR transferability studies in Vetiver grass (Vetiveria zizanioides L. Nash).
- Author
-
Singh, Rakesh, Narzary, Diganta, Bhardwaj, Jyoti, Singh, Amit Kumar, Kumar, Sundeep, and Kumar, Ashok
- Subjects
- *
VETIVER , *PLANT genetics , *PLANT diversity , *BIOMARKERS , *PLANTS , *PLANT germplasm - Abstract
Highlights: [•] This is first comprehensive analysis of the genetic diversity at molecular level among Indian vetiver germplasm, using RAPD and ISSR markers. [•] The cluster analysis and AMOVA has indicated that how human intervention and clonal method of propagation has affected the variation both at population and individual level respectively. [•] SSR marker has been developed in vetiver using cross species transferability. These markers can be potentially utilized for locus specific characterization and genetic improvement of vetiver germplasm. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.