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Background. Recently, the number of patients with obesity and metabolic syndrome has increased. Numerous studies to find new regulators of metabolic processes have led to the discovery of a new fibroblast growth factor 21 (FGF21). The purpose of the study was to determine the correlation between FGF21 and metabolic processes among patients with gastrointestinal pathology. Materials and methods. The study was conducted in 80 patients with gastrointestinal diseases aged 18 to 66 years, the average age was (39.04 ± 1.03) years. Among the subjects, there were 56 men (70.0 %) and 24 women (30.0 %). Body composition was assessed using the Tanita MC-780MA apparatus (Japan). Serum leptin, FGF21, insulin levels were measured by enzyme-linked immunosorbent assay, and the HOMA-IR was calculated. Free fatty acids in the serum of patients were evaluated on Chromateс Crystal-5000, a gas chromatograph with a flame ionization detector. Results. The study found positive correlations between FGF21 levels and indicators of bioimpedance analysis: body mass index (r = 0.24; p = 0.004), fat mass (r = 0.26; p = 0.01) and visceral fat (r = 0.29; p = 0.01). In addition, a relationship was detected between FGF21 levels and laboratory data: insulin (r = 0.32; p = 0.001), HOMA-IR (r = 0.31; p = 0.001), leptin (r = 0.32; p = 0.001), triglyceride/ high-density lipoprotein ratio (r = 0.48; p = 0.0001), and the content of certain free fatty acids. This indicates that FGF21 affects the development of obesity and metabolic disorders. It was found that FGF21 is associated with visceral obesity in patients with digestive diseases (odds ratio = 1.28, p = 0.0022). FGF21 levels above 1,317.5 pg/mL (sensitivity of 71.0 %, specificity of 53.1 %) indicate the presence of visceral obesity in patients with gastrointestinal diseases. Conclusions. As a result of a comprehensive study, new data were obtained on the role of FGF21 as a key regulator of metabolic disorders, which is confirmed by the established relationships of this indicator with the components of lipid and carbohydrate metabolism disorders, cytokine imbalance, and hormonal activity of adipose tissue in patients with pathology of the digestive system. [ABSTRACT FROM AUTHOR]

Introduction: Enthesitis-related arthritis (ERA) is one of the types of Juvenile Idiopathic Arthritis (JIA). ERA accounts for 11-16% of JIA cases. Aim: To present both established and potential prognostic and activity markers in children and adolescents with ERA. Methods: Determining disease activity and prognosis is of great importance for the clinician‘s assessment and therapeutic approach. So far, no universal marker has been established to determine the activity of JIA. The JADAS scale, JSpADA, laboratory markers such as CRP, ESR are widely used. Although less frequently, other markers of inflammation are also used: fecal calprotectin, matrix metalloproteinases, interleukins. The main prognostic markers for ERA are the number and type of affected joints, the involvement of the axial skeleton. An innovation in markers of activity and prognosis in JIA, including ERA, are microRNAs. Results: Many studies worldwide in the last decade have demonstrated the potential of some microRNAs as epigenetic markers of activity and prognosis in ERA patients. Conclusion: Тhe therapeutic approach in patients with ERA is based on markers of activity and the presence of poor prognostic markers. Validation of some microRNAs as prognostic markers and markers of activity is a new and still not well known path to earlier diagnosis and treatment. [ABSTRACT FROM AUTHOR]

The authors conducted a literature review of the current state of immunohistochemical diagnostics in determining the durability and time of occurrence of mechanical damage in forensic medical practice. Problems in the selection of immunohistochemical markers and the need to create immunohistochemical panels for qualitative research and definitive results were revealed. The authors recommend further scientific and practical research to improve knowledge and methods that should be implemented for routine research in forensic medical practice. [ABSTRACT FROM AUTHOR]

The study of intraspecific genetic diversity of cattle, including species of the genus Bubalus from the subfamily Bull, is important because of the reduction of biodiversity of farm animals. The basis of genetic diversity is its genetic component. The loss of native species and breeds of cattle is a real threat to the biosphere, as the resilience of natural ecosystems and agroecosystems is directly linked to their genetic ability to adapt to environmental conditions. Polymorphism of ISSR-markers of the Ukrainian population of water buffaloes (Bubalus bubalis) from the farm of “TASBIO” LLC (Chernihiv region) was analyzed in 66 animals selected for the study. Genomic DNA samples were isolated from venous blood with a standard set of reagents. Genotyping was performed using specific ISSR primers: (ACC)6G, (GAG)6C, (AG)9C, (CTC)6C, (AG)8CA, (AG)8CGand (GA)6CC. We determined the difference in spectra both by the number of amplicons, their lengths (number of nucleotides) and by their polymorphism. As a result of the study, all primers showed polymorphism of buffalo DNA regions. Amplicons were defined in the range from 200 bp up to 4000 bp. Analysis of ISSR spectra revealed 87 loci, of which 71 were polymorphic. (AG)8CA-marker was the least polymorphic (PIC=0.234), (CTC)6C the most polymorphic (PIC=0.389). Conservative loci were found in four ISSR markers: 6 in (AG)8CA marker, 5 in (AG)8CG, 4 in (GA)6CC, and 1 in (AG)9C. 67 species-specific loci were identified for the Ukrainian water buffalo population: 10 for (AG)9C, 3 loci for (ACC)6G, 4 for (GAG)6C, 7 for (CTC)6C, 15 for (AG)8CA, and 14 in (AG)8CG and (GA)6CC. The ISSR primers used are recommended for molecular genetic analysis of buffalo DNA polymorphism.

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Існують різні способи реалізації доповненої реальності. Найнадійнішим і найстабільнішим залишається маркерний спосіб. Зроблено спробу з’ясувати, яка з існуючих маркерних систем краща, точніша, надійніша, зручніша. Виконано порівняння існуючих маркерних систем, у яких використовуються візуальні маркери, що шифруються за допомогою площинних квадратів. Оцінку проведено на основі таких критеріїв, як зручність, ефективність, точність та надійність. Методики, які застосовувалися під час експериментів: визначення часу оброблення для виявлення та декодування маркера, точність визначення особливих точок, розпізнавання маркера у випадку проективних спотворень, розпізнавання декількох маркерів, розпізнавання з малою ділянкою маркера на зображенні, розпізнавання в умовах поганого фокуса зображення. Для порівняння використано чотири системи розпізнавання маркерів. Подано результати оцінювання як якісного, так і кількісного щодо зручності використання, ефективності, точності та надійності таких систем. Це забезпечує аналіз переваг та недоліків різних аспектів систем відстеження маркерів.

Copyright of System Research & Information Technologies / Sistemnì Doslìdžennâ ta Ìnformacìjnì Tehnologìï is the property of Institute for the Applied System Analysis at the NTUU KPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Obtaining highly homogeneous mixtures is an urgent task in many areas of feed production, as it is associated with the need for even distribution of particularly important and valuable components. Manufacturers of animal feed are using several different methods for performing homogeneity studies to check their mixer performance. This paper is devoted to the use of nontraditional markers such as ferromagnetic Microtracers to evaluate the homogeneity of the final feed. Microtracers have successfully been used in a mixer testing program in the USA since 1985 and in Europe since 2006. They represent a major improvement over traditional analytical procedures for evaluation of mixer performance involving the use of cobalt or manganese salts. The authors described the quantitative results of determination and identification of Microtracers in two sets of 20 samples of pet food manufactured by Kormotech LLC with using a proprietary Rotary Detector procedure developed by Micro-Tracers Inc (San Francisco, California). It was shown that analysis of 150 g samples at level of Microtracer addition of 20 g per metric ton of pet food allowed a magnetic retrieval of the number of ferromagnetic particles from 61 to 101 which was sufficient for application of Poisson and Chi-squared statistics. The obtained results from two tests performed with different time of mixing were interpreted in accordance with the requirements of the GMP + BA2 standard. It was determined that mixing within 3 min leads to the marginal mixing with the calculated probability value of 1.12%. The prolonged mixing within 4 min leads to complete mixing with the calculated probability value of 5.93%. The use of ferromagnetic Microtracers in a mixer testing program is justified for such tasks as comparison of mixers with each other, as well as identifying the changes in the technical characteristics of the mixers and their performance occurring during their exploitation. In addition, using microtracers can quickly determine the quality of the mixing equipment when it is purchased. [ABSTRACT FROM AUTHOR]

Copyright of International Dialogue: East-West is the property of International Center for Slavic Education- Sveti Nikole and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Gastroenterologia (2308-2097) is the property of Zaslavsky O.Yu and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)