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The carbon/phenolic composites are extensively considered as an efficient ablative thermal protection material, but inferior oxidation-ablation resistance has limited their further application. In this work, a multiscale ZrO 2 -SiOC coated carbon/phenolic composite (NCF/ZSAx-PA) was fabricated through a dual-stage construction strategy. The as-prepared composites exhibited a low density (0.314–0.348 g/cm3), low thermal conductivity (0.0605–0.0725 W/(m·k)) and promoted compressive strength (2.95/5.08 MPa in the Z/XY direction). Benefiting from the formation of a protective ZrO 2 -SiO 2 ceramic coating, the multiscale composites showed excellent anti-oxidation and ablation performance. The residual weight of NCF/ZSAx-PA was 19.41 % at 1000 °C while the unmodified carbon/phenolic composites were entirely burnt off under an air atmosphere. Besides, the NCF/ZSAx-PA exhibited low back temperature (53.6 °C), low linear ablation rate (0.0519 mm/s), and low mass loss rate (0.0100 g/s) under the 3.62 MW/m2 oxyacetylene assessment. All the superior properties enabled the NCF/ZSAx-PA a prospective application on the thermal protection system. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the antioxidant effect of alginate oligosaccharides (AOS) on sweet basil leaves preserved at low temperatures. This investigation was divided into 3 treatments and 3 replications using a completely randomized design (CRD) experiment. Specifically, the effects of dipping basil leaves in AOS solutions at different concentrations (100 and 200 mg L-1) and in distilled water (control) for 1 min were compared. Cell deterioration due to cold storage was investigated based on the variation in oxidative reactions in the cells, causing an accumulation of free radicals. The AOS-treated sweet basil leaves had a 12-day shelf life, compared to just 9 days for those in the control group. Those treated with AOS at 100 mg L-1 exhibited the lowest levels of phenylalanine ammonia-lyase, lipoxygenase, hydrogen peroxide, peroxidase and malondialdehyde content. On the contrary, the contents of phenolic compounds, polyphenol oxidase, catalase and superoxide dismutase increased in the treated leaves during storage compared to those observed in the control treatment. [ABSTRACT FROM AUTHOR]

Background: Pathogenic infections can significantly impact the health of livestock. Traditionally, antibiotic growth promoters (AGPs) have been used in feed to enhance growth performance and disease control. However, concerns regarding antibiotic resistance have led to the exploration of traditional herbal medicine as a natural alternative, guided by the principle of medicine-food homology. The Taguchi method was employed to optimize the culture formula for cordycepin production, an active component of Cordyceps militaris (C. militaris). The influences of C. militaris supplementing solid-state fermentation (CMSSF) in feed on the growth performance and immune responses of grower pigs were evaluated in the present study. Results: The C. militaris ethanol extract (CME) displayed potent free radical scavenging activity against 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) after undergoing fermentation. Additionally, the antibacterial testing revealed that CME effectively inhibits the growth of common pig pathogens such as Glaesserella parasuis, Pasteurella multocida, Staphylococcus hyicus, and Streptococcus suis. In lipopolysaccharide (LPS)-treated intestinal porcine enterocyte cell line (IPEC-J2), CME significantly suppressed the production of pro-inflammatory cytokines, including tumor necrosis factor (TNF)-α and interleukin (IL)-6. In addition, higher antioxidative activity was detected as indicated by elevated concentration of superoxide dismutase (SOD) in pig serum. The levels of immunoglobulin M (IgM), IgA, and IgG antibodies, as well as classical swine fever virus (CSFV) antibodies (S/P ratio) in serum were all increased. Growth performance of pigs fed with dietary CMSSF supplementation was improved in comparison with the control. Conclusions: Results demonstrated that CMSSF has the potential to be used as a natural growth promoter to enhance immunity, antioxidation, as well as overall health and growth performance of grower pigs. [ABSTRACT FROM AUTHOR]

The continuous imbalance of the diabetic wound microenvironment is an important cause of chronic nonhealing, which manifests as a vicious cycle between excessive accumulation of reactive oxygen species (ROS) and abnormal healing. Regulating the microenvironment by suppressing wound inflammation, oxidative stress, and bacterial infection is a key challenge in treating diabetic wounds. In this study, ROS‐responsive hydrogels are developed composed of silk fibroin methacrylated (SFMA), modified collagen type III (rCol3MA), and lipid nanoparticles (LNPs). The newly designed hydrogel system demonstrated stable physicochemical properties and excellent biocompatibility. Moreover, the release of antimicrobial peptide (AMP) and puerarin (PUE) demonstrated remarkable efficacy in eradicating bacteria, regulating inflammatory responses, and modulating vascular functions. This multifunctional hydrogel is a simple and efficient approach for the treatment of chronic diabetic infected wounds and holds tremendous potential for future clinical applications. [ABSTRACT FROM AUTHOR]

Alzheimer's disease (AD) involves a complex pathophysiology with multiple interconnected subpathologies, including protein aggregation, impaired neurotransmission, oxidative stress, and microglia-mediated neuroinflammation. Current treatments, which generally target a single subpathology, have failed to modify the disease's progression, providing only temporary symptom relief. Multi-target drugs (MTDs) address several subpathologies, including impaired aggregation of pathological proteins. In this review, we cover hybrid molecules published between 2014 and 2024. We offer an overview of the strategies employed in drug design and approaches that have led to notable improvements and reduced hepatotoxicity. Our aim is to offer insights into the potential development of new Alzheimer's disease drugs. This overview highlights the potential of multi-target drugs featuring heterocycles with N-benzylpiperidine fragments and natural compounds in improving Alzheimer's disease treatment. [ABSTRACT FROM AUTHOR]

Butterfly pea flower (BPF), roselle calyx (RC), and grape skin (GS) are rich in bioactive phenolics with health benefits. Due to its simplicity, safety, and environmental friendliness, this study used water as a solvent to explore different extraction conditions in these plant materials and compared the heat stability of anthocyanins in the aqueous extracts. To maximize the total anthocyanins and polyphenols in the aqueous extracts, the powders of BPF, GS, and RC should be extracted for 30 min at 90 °C; 30 min and 120 min at 90 °C; and 30 min and 60 min at 60 °C, respectively. Among the tested plant materials, the content of total anthocyanins was RC > GS > BPF, while the total phenolic content was GS > BPF > RC. Anthocyanins of the aqueous extracts underwent rapid thermal degradation at high temperatures and high pH values. The thermal stability of anthocyanins in the materials was in the order: BPF > GS > RC. This is likely related to the types and structures of the anthocyanins such as the degree of acylation and glycosylation. The study demonstrates that hot water extraction is efficient and practical for these materials, yielding extracts suitable for food and nutraceutical applications. [ABSTRACT FROM AUTHOR]

Onions (Allium cepa L.) are nutritious vegetables; however, variations in processing methods can influence their chemical composition and functional properties. Raw processing and cooking are the two main food-processing methods for onions, but it is not clear what kind of changes these two methods cause. In the present study, ultrahigh-resolution liquid chromatography–mass spectrometry (UHPLC–MS) was utilized to observe the changes in onion composition during cooking and to investigate the protective effects of raw and cooked onion extracts against lead damage in vitro and at the cellular level. Many compounds were identified, including amino acids, nucleosides, flavonoids, and organosulfur compounds. Cooking causes changes in the content of numerous amino acids (e.g., DL-glutamine) in onions and increases nucleoside content (e.g., 5'-S-methyl-5'-thioadenosine, adenine). Both raw and cooked onion extracts can reduce neuronal cell damage caused by lead exposure, but cooking increased the free radical scavenging (e.g., DPPH, ABTS, hydroxyl radicals) and chelating of lead ions (up to about 25%) of the onion extracts. In conclusion, cooking can cause changes in the chemical composition of onions and increase their antioxidant and lead chelating capacity. [ABSTRACT FROM AUTHOR]

Metabolic‐associated fatty liver disease (MAFLD) has become one of the most widespread metabolic diseases nowadays, while no definitive agent is approved for practical treatment. Given excessive lipid deposition and cellular oxidative stress are two main inducers of MAFLD, developing synergistic therapeutic agents that enhance lipid metabolism and antioxidative ability are effective routes for MAFLD therapy. Herein, a novel kind of bio‐functional carbon dots (MTCDs) derived from metformin and tea polyphenols based on a fused pharmacophore strategy is developed. The surface of MTCDs retains abundant phenolic hydroxyl and guanidine groups, which are designed to reduce both lipid deposition and oxidative stress in the liver. Moreover, by finely controlling the surface charge, MTCDs exhibit excellent lysosome targeting functionality with favorable biocompatibility and low biotoxicity. Mechanistical studies further reveal that MTCDs treatment protects lysosomes from oxidative stress damage and activates hepatocyte AMP‐activated protein kinase signaling, which initiates lipophagy progress to accelerate lipolysis and lipid consumption. Collectively, these studies propose an effective "two birds with one stone" tool for MAFLD synergistic therapy and suggest a viable strategy for multi‐pharmacophore agent development. [ABSTRACT FROM AUTHOR]

Background: Controlled non-enzymatic glycation reactions are common under normal physiological conditions. However, during elevated blood glucose conditions, the glycation reactions are accelerated, leading to the formation of toxic compounds such as advanced glycation end products (AGEs). Several natural products are now being investigated as protective agents against glycation to preserve blood protein structure and functions. Methods: Human serum albumin (HSA) was glycated with 0.05 M α-D-glucose alone or in the presence of Zingiber officinale Roscoe (ginger) extract (0.781–100 μg/mL) for 10 weeks, and biochemical, biophysical, and computational analyses were carried out. Results: HSA glycated for 10 weeks (G-HSA-10W) resulted in significant production of ketoamines, carbonyl compounds, and AGE pentosidine. Notable structural alterations were observed in G-HSA-10W, ascertained by ultraviolet (UV), fluorescence, and circular dichroism (CD) studies. Antioxidant, anti-glycating, AGEs inhibitory, and antibacterial effects of ginger extracts were observed and attributed to the presence of various phytochemicals. Molecular docking studies suggested that the compounds 8-shagaol and gingerol exhibited strong and multiple interactions with HSA. Molecular simulation analysis suggests HSA attains a high degree of conformational stability with the compounds gingerol and 8-shogaol. Conclusions: These findings showed that ginger extract has an antioxidant function and can prevent glycation-induced biochemical and biophysical alterations in HSA. Thus, aqueous ginger extract can be utilized to combat glycation and AGE-related health issues, especially diabetes, neurological disorders, inflammatory diseases, etc. [ABSTRACT FROM AUTHOR]

Vitiligo is a chronic autoimmune disorder characterized by progressive skin depigmentation. Vitiligo significantly impacts patients’ quality of life, contributing to psychological and social burdens. Despite readily available therapeutic options, many cases remain refractory to treatment, highlighting the critical need for safer and more effective therapies. Currently, ruxolitinib is the only FDA-approved medication for vitiligo; however, it carries a black box warning for serious adverse effects, including infections, malignancy, and major cardiovascular events, limiting its use. Recent studies have identified the aryl hydrocarbon receptor (AhR) as a promising therapeutic target, suggesting that AhR agonists could address the multifaceted pathogenesis of vitiligo. Adhering to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, we conducted a comprehensive search to analyze the role of AhR agonists in the treatment of vitiligo on PubMed, Cochrane, Embase, MEDLINE, and Web of Science databases on April 15, 2024. Fourteen studies met the inclusion criteria, comprising two clinical trials, two case reports, and nine basic science studies. Our search revealed that culturing AhR agonists with melanocytes upregulates melanin-synthesizing enzymes, reduces reactive oxygen species, and modulates pro-inflammatory cytokines such as IL-17A and IL-22. Tapinarof, a topical AhR agonist used commonly for the treatment of psoriasis, demonstrated clinical efficacy in repigmentation with a favorable safety profile compared to long-term steroid use. Although limited by the number of clinical studies, this review underscores the potential of using AhR agonists, such as tapinarof, as a transformative approach to vitiligo management. Future clinical trials are necessary to evaluate the safety, efficacy, and long-term outcomes of AhR agonists. [ABSTRACT FROM AUTHOR]

Kombucha, a functional beverage rich in glucuronic acid, is fermented in the presence of acetic acid bacteria and yeast as the primary microorganisms. Glucuronic acid is recognized for its various physiological benefits, such as detoxification, antioxidation, and anti-inflammation. To optimize the glucuronic acid content in kombucha, various strain combinations by selecting fermented sources were accomplished. According to the experimental results, kombucha produced through co-fermentation with Pichia anomala and Komagataeibacter hansenii, with glucose-added black tea as the carbon source, exhibited the highest glucuronic acid production. A response surface methodology found that under optimized conditions of a 12.27% (w/v) carbon source concentration, a 10.07% (w/v) substrate concentration, and a 28.4 °C temperature, the highest glucuronic acid production reached 80.16 g/L, which represented a 2.39-fold increase compared to the original kombucha. Furthermore, the total polyphenol content increased by 3.87-fold, while DPPH and ABTS free radical–scavenging capacities increased by 1.86- and 2.22-fold, respectively. To sum up, these observations reveal the potential for commercial production of glucuronic acid–enriched kombucha and contribute to the development of functional food products related to kombucha in the future. [ABSTRACT FROM AUTHOR]

Copyright of China Condiment is the property of China Condiment and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Condiment is the property of China Condiment and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Endogenous electric field (EEF) is weakened in wounds due to electrolyte loss, hindering wound repair. Reshaping and repairing EEF play important roles in accelerating wound healing. The design of antibacterial dressings coupled with an EEF recovery ability to promote wound healing is urgent and significant. Herein, a self‐powered wearable thermoelectric dressing with antibacterial, antioxidant, and EEF reshaping activities for methicillin‐resistant Staphylococcus aureus‐infected wound therapy is fabricated. The dressing is based on a wearable thermoelectric device constructed of a polyanionic thermoelectric hydrogel loaded with tannic acid (TA). By taking advantage of the natural temperature difference between infected wounds and the external environment, the dressing is activated to convert thermal energy into electricity, producing a continuous and compensating electric field to reshape damaged EEF and stimulate tissue regeneration. Combined with the antibacterial and antioxidant properties of the TA released from the thermoelectric hydrogel, the self‐powered dressing can accelerate wound healing in vivo by topical targeted sterilization, reducing inflammation and reshaping the EEF of the wounds. Thus, this dressing provides a promising and facile self‐powered therapeutic strategy without the need for external stimuli to treat infected wounds. [ABSTRACT FROM AUTHOR]

Introduction: The aim of this research was to clarify the mechanism through which baicalin exerts its inhibitory effects on Aeromonas hydrophila infection. Methods: The antibacterial efficacy of baicalin was assessed by determining its minimum inhibitory concentration (MIC) against A. hydrophila. Various parameters, including the growth curve, cell wall integrity, biofilm formation, AKP content, and morphological alterations of A. hydrophila, were analyzed. In vivo experiments involved the administration of A. hydrophila 4 h postintraperitoneal injection of varying doses of baicalin to induce infection, with subsequent monitoring of mortality rates. After a 3 d period, liver, spleen, and intestinal tissues were harvested to evaluate organ indices, antioxidant and immune parameters, as well as intestinal microbial composition. Results: The findings indicated that baicalin treatment resulted in the disruption of the cell wall of A. hydrophila, leading to the loss of its normal structural integrity. Furthermore, baicalin significantly inhibited biofilm formation and facilitated the release of intracellular proteins (P < 0.05). In vivo, baicalin enhanced the survival rates of yellow catfish infected with A. hydrophila. Compared to the control group, the liver index of yellow catfish was elevated, while the spleen and intestinal indices were reduced in the baicalin-treated group (P < 0.05). Additionally, baicalin at an appropriate dosage was found to increase levels of SOD, GSH, CAT, ACP, and AKP in yellow catfish (P < 0.05), while simultaneously decreasing MDA accumulation and the mRNA expression of inflammatory markers such as Keap1, IL1, IFN-g, and TNF-a, (P < 0.05). Moreover, baicalin significantly enhanced the operational taxonomic unit (OTU) count in A. hydrophila-infected yellow catfish (P < 0.05), restoring the abundance of Barnesiellaceae, Enterobacteriaceae, Plesiomonas, and UBA1819 (P < 0.05). Discussion: In summary, baicalin demonstrates the potential to improve the survival rate of yellow catfish subjected to A. hydrophila infection, augment antioxidant and immune responses, mitigate inflammation, and enhance intestinal microbial diversity. [ABSTRACT FROM AUTHOR]

This research aimed to investigate the effects of dietary fructooligosaccharides (FOS) on attenuating the Aeromonas hydrophila (A. hydrophila)-induced oxidative stress and apoptosis in blunt snout bream Megalobrama amblycephala. Fish were divided into three groups as follows: C1 (Control), T1 (A. hydrophila), and T2 (A. hydrophila + 4 g/kg FOS). The results showed that the activities of antioxidant enzymes increased, the liver morphology had disorderly arrangement, and extensive cell necrosis occurred because of A. hydrophila-infection. While the dietary FOS improved the above-mentioned liver damage. Additionaly, FOS elevated mRNA levels of pro-apoptotic molecules, including caspase-8 and 9, and down-regulated mRNA levels of the anti-apoptotic molecule Bcl-2, which is triggered by A. hydrophila-infection. The transcriptome analysis showed that the oxidative stress-related DEGs pathways were activated in intestine of blunt snout bream by A. hydrophila-infection. The FOS-added group led to the enrichment of more pathways to health. Further WGCNA co-expression network analysis showed that the screened single genes were clustered into 49 modules. The two modules with the highest association to the five traits (10 hub genes) were chosen to build the network by combining the physiological and biochemical characteristic. In summary, this research offers a foundation for the exploring of A. hydrophila-restoration genes in dietary FOS, and also lays a theoretical foundation for aquaculture in the future. [ABSTRACT FROM AUTHOR]

The myofibrillar protein (MP) of duck meat is prone to excessive oxidation during thermal processing, resulting in a decline in its overall quality. In this paper, the effect of shikimic acid on the oxidative structure of duck muscle fibrin was studied. The findings showed that, at a mass ratio of 1:50,000 (g/g) between shikimic acid and MP, the carbonyl content of MP was reduced by 74.20%, while the sulfhydryl content was increased by 73.56%. MP demonstrated the highest denaturation temperature, whereas its thermal absorption was the lowest. The percentage of α-helixes and β-sheets increased by 16.72% and 24.74%, respectively, while the percentage of irregular structures decreased by 56.23%. In addition, the surface hydrophobicity index of MP exhibited a significant decrease (p < 0.05), while there was a significant increase in its free radical-scavenging ability (p < 0.05). Molecular fluorescence spectrum analysis showed that shikimic acid could bind to MP, altering the internal environment of MP and enhancing its thermal stability. FTIR analysis showed that shikimic acid could enhance the distribution of protein particle sizes by reducing irregular structures, the proportion of β-rotation, and the degree of protein aggregation. It is hoped that this research can offer scientific support for improving meat processing technology. [ABSTRACT FROM AUTHOR]

The aging process is influenced by the accumulation of both beneficial and harmful factors in the external environment. While extensive literature has explored the health benefits of short-term forest bathing, the potential for long-term exposure to forests to delay the aging process remains unclear. This study aimed to investigate the impact of prolonged urban forest contact on aging using a murine model. Forty male Specified Pathogen Free degree Kunming mice (6 weeks old, average weight 30.44 ± 0.91 g) were subjected to continuous subcutaneous injection of D-galactose in an urban environment and an urban forest environment to simulate an accelerated aging process lasting six weeks. Results showed that compared to urban environment, mice living in the forest environment exhibited significantly increased levels of serum oxidases CAT, GSH-Px, and SOD, as well as decreased levels of oxidative products H2O2 and MDA. These findings suggest that long-term forest living enhances serum Nrf2 expression, downstream oxidase activity, total antioxidant capacity, and resistance to free radicals and oxidative products, thus delaying the aging process. This study offers valuable insights into the potential health benefits of long-term forest living, which may play a role in delaying the aging process in humans. [ABSTRACT FROM AUTHOR]

Control of oxidation/antioxidation homeostasis is important for cellular protective functions, and disruption of the antioxidation balance by exogenous and endogenous ligands can lead to profound pathological consequences of cancerous commitment within cells. Although cancers are sensitive to antioxidation drugs, these drugs are sometimes associated with problems including tumor resistance or dose-limiting toxicity in host animals and patients. These problems are often caused by the imbalance between the levels of oxidative stress-induced reactive oxygen species (ROS) and the redox efficacy of antioxidants. Increased ROS levels, because of abnormal function, including metabolic abnormality and signaling aberrations, can promote tumorigenesis and the progression of malignancy, which are generated by genome mutations and activation of proto-oncogene signaling. This hypothesis is supported by various experiments showing that the balance of oxidative stress and redox control is important for cancer therapy. Although many antioxidant drugs exhibit therapeutic potential, there is a heterogeneity of antioxidation functions, including cell growth, cell survival, invasion abilities, and tumor formation, as well as the expression of marker genes including tumor suppressor proteins, cell cycle regulators, nuclear factor erythroid 2-related factor 2, and Jun dimerization protein 2; their effectiveness in cancer remains unproven. Here, we summarize the rationale for the use of antioxidative drugs in preclinical and clinical antioxidant therapy of cancer, and recent advances in this area using cancer cells and their organoids, including the targeting of ROS homeostasis. [ABSTRACT FROM AUTHOR]

Osteoarthritis (OA) is a prevalent joint disease characterized by cartilage degeneration and subchondral bone homeostasis imbalance. Effective topical OA therapy is challenging, as therapeutic drugs often suffer from insufficient penetration and rapid clearance. We develop miniature polydopamine (PDA) nanocapsules (sub-60 nm), which are conjugated with collagen-binding polypeptide (CBP) and loaded with an anabolic drug (i.e., parathyroid hormone 1–34, PTH 1–34) for efficient OA treatment. Such multifunctional polymeric nanocapsules, denoted as PDA@CBP-PTH, possess deformability when interacting with the dense collagen fiber networks, enabling the efficient penetration into 1 mm cartilage in 4 h and prolonged retention within the joints up to 28 days. Moreover, PDA@CBP-PTH nanocapsules exhibit excellent reactive oxygen species scavenging property in chondrocytes and enhance the anabolism in subchondral bone. The nanosystem, as dual-mode treatment for OA, demonstrates rapid penetration, long-lasting effects, and combinational therapeutic impact, paving the way for reversing the progression of OA for joint health care. [Display omitted] • Miniature, uniform, and deformable PDA nanocapsule system was developed. • The nanocapsules penetrated 1 mm of cartilage within 4 h. • The nanocapsules exhibited prolonged retention in knee joints for up to 28 days. • Dual therapeutic effects included enhanced antioxidant and bone anabolism. [ABSTRACT FROM AUTHOR]

Objectives: The aim of this study was to compare cortisol concentrations, nutritional composition, and the antioxidant status of human milk of women living in Bydgoszcz (Poland), taking into account maternal factors (fertility, area of residence, economic activity, and breastfeeding period). Methods: The basic composition of human milk was evaluated using the MIRIS HMATM analyzer. The level of cortisol was determined by the enzyme-linked immunosorbent method. In order to determine the antioxidant activity, the DPPH radical method was used. Results: It was observed that the concentration of cortisol in human milk in the group of women living in the city center was higher compared to the milk of women living on the outskirts of the city. In the group of women breastfeeding from 3 to 5 weeks after childbirth, the concentration of cortisol in milk was higher compared to the group of women breastfeeding less than 12 months of age and compared to the group of women lactating over 12 months of age. The antioxidant status of human milk was highest in the group of professionally active women and in the group of breastfeeding women from 3 to 5 weeks after childbirth. The basic composition and the caloric value of human milk differed statistically significantly in the study groups. Conclusions: Based on this study, it can be concluded that the composition and antioxidant status of human milk depends on maternal factors (fertility, professional activity, area of residence, and breastfeeding period). Higher cortisol concentrations in breast milk are probably determined by the area of residence (city center and associated higher noise/sound and stress levels) and lactation period (hormonal imbalance, fatigue, and postpartum period). Milk from economically active women shows greater protection against reactive oxygen species compared to milk from inactive women, protecting against the occurrence of diseases of civilization. Milk from breastfeeding women over 12 months of age also shows protection against reactive oxygen species, despite the fact that the highest level of antioxidant status of human milk occurs in the initial period of lactation. [ABSTRACT FROM AUTHOR]

Copyright of China Condiment is the property of China Condiment and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Condiment is the property of China Condiment and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Condiment is the property of China Condiment and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study was designed to explore the hepatoprotective effects of enzymatic degradation products derived from sturgeon roe on alcohol-damaged hepatocytes and to elucidate the underlying mechanisms. Utilizing sturgeon roe as the research material, the enzymatic process was optimized by focusing on key parameters such as enzymatic efficiency and in vitro activity. The protective effects on hepatocytes were systematically investigated by using the HepG2 cell model. Advanced computerized virtual screening technology was employed to identify potential bioactive peptides within the sturgeon roe. The findings indicated that the optimum enzymatic product of sturgeon roe was obtained under the conditions of 1:1 compounding of alkaline protease (1343 U/g) and trypsin (27 U/g) for 8 h, yielded a protein recovery of 49.30%±0.57%, a hydrolysis degree of 46.05%±0.92%, and ABTS+ free radical scavenging rate of 51.45%±0.66%. Notably, at a concentration of 5 mg/mL, this product significantly enhanced the activity of alcohol dehydrogenase (ADH) by 168.64%±1.42% (P<0.05). The product prepared under this enzymatic condition at 0.3~2 mg/mL significantly elevated superoxide dismutase (SOD) activity, glutathione (GSH) contents, and reduced malondialdehyde (MDA) contents in alcohol-injured HepG2 cells compared to the model group (P<0.05). In addition, the product at 0.3~2 mg/mL also significantly reduced alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities (P<0.05). Virtual screening identified 5 peptides, including LPG and FLPR, in the sturgeon roe enzymatic degradation product, showed promising hepatoprotective properties against alcohol-induced hepatocyte injury. Consequently, this study lays a solid foundation for the further development and utilization of aquatic resources, such as sturgeon roe, in hepatoprotective applications. [ABSTRACT FROM AUTHOR]

Protein-stabilized star anise essential oil nano-emulsion (SAEO-NE) was successfully prepared using an ultrasound-assisted method to address the intrinsic defects and enhance the bioavailability of star anise essential oil (SAEO). In this study, the polydispersity index (PDI), particle size, and zeta potential (ZP) of SAEO-NE were used as performance indicators, and the optimal preparation conditions were determined to be an emulsifier dosage of 5%, an ultrasonic power of 270 W, and an ultrasonic time of 15 min. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) analyses revealed that the resulting nanoparticle exhibited a uniform spherical shape, with a particle size of 246.5 ± 1.35 nm and a ZP of -38.97 ± 0.45 mV. Rheological analyses further confirmed the favorable rheological characteristics of the nano-emulsion. Moreover, SAEO-NE demonstrated robust adaptability across varying salinities (100, 200, 300, and 400 mM) and pH levels (1, 3, 5, 7, 9). After storage at 4 °C for 5 weeks, the nano-emulsion maintained particle sizes below 300 nm, indicating excellent storage stability. In addition, the in vitro antioxidant evaluation showed that SAEO exhibited enhanced antioxidant activity after nano-emulsification. [ABSTRACT FROM AUTHOR]

In this paper, honeysuckle was used as raw material to compare the effects of hot water extraction (HWE), enzyme extraction (EE), ultrasonic-assisted extraction (UE) and ultrasonic-enzyme extraction (UEE) on the physicochemical properties and antioxidant activity of honeysuckle polysaccharides. The results showed that the yield of polysaccharide extracted by the four methods from high to low was UE-LJP (7.74%), EE-LJP (4.35%), UEE-LJP (3.38%) and HWE-LJP (2.13%). The polysaccharides obtained by different extraction methods all contain uronic acid, and the polysaccharide was an acidic polysaccharide. The ultrasonic-assisted extraction of polysaccharide from Lonicerae japonicae Flos had the lowest protein content (0.16%), the strongest water holding capacity and hydration characteristics. The total sugar content of polysaccharide extracted by enzymatic method was higher (38.68%). The antioxidant activity showed that the four polysaccharides had strong antioxidant activity. Among them, HWE-LJP, UE-LJP and EE-LJP had strong DPPH free radical scavenging ability, and the half inhibitory concentration was 0.067, 0.104 and 0.093 mg/mL, respectively. In terms of hydroxyl radicals, UEE-LJP showed strong scavenging ability, and the half inhibitory concentration was 0.390 mg/mL. Therefore, different extraction methods had significant effects on the physical and chemical properties and antioxidant activity of Lonicerae japonicae Flos polysaccharides. UE method and EE method could be used as the most suitable methods for extracting Lonicerae japonicae Flos polysaccharides. This paper would provide reference for the development of functional food and antioxidant and other functional drug raw materials.

Objectives: This study was to optimize the extraction and purification process parameters of donkey serum albumin (DSA) from Asini Corii Colla, to compare the emulsification, foaming properties and antioxidant activity of DSA before and after purification, and to identify the purity of DSA. Methods: In this study, Asini Corii Colla was used as raw material and DSA extraction rate was used as the response value, the optimum extraction process of salting-out-ultrasonic assisted extraction was determined by single factor and Box-Behnken response surface design. The concentration of DSA in Asini Corii Colla was purified by Sephadex G-75, and the purity of DSA in purified Asini Corii Colla was identified by SDS-PAGE gel electrophoresis. The water holding capacity, oil holding capacity, emulsification and foaming properties of DSA in Asini Corii Colla before and after purification were compared. Finally, DSA was used to analyze its antioxidant activity in vitro by scavenging ability of superoxide anion radical, ABTS+ radical, DPPH radical and hydroxyl radical. Results: The optimum extraction process parameters of DSA in Asini Corii Colla were as follows: The ratio of dissolved solution was 1:11 g/mL, the ratio of material to liquid was 1:8.3 g/mL, the ultrasonic power was 400 W, and the ultrasonic time was 31 minutes. Under these conditions, the extraction rate of DSA was 49.57%, and the purity of DSA after purification could reach 83.7%. The results of functional properties showed that the water holding capacity, oil holding capacity, emulsification and foaming properties of DSA purified under different pH conditions were enhanced compared with those before purification. The results of antioxidant activity in vitro showed that DSA had strong antioxidant activity in vitro. When the concentration of DSA was 10.0 mg/mL, the scavenging rates of superoxide anion radical, ABTS+ radical, DPPH radical and hydroxyl radical were 73.39%, 83.61%, 71.24% and 83.79%, respectively. Conclusions: The extraction, purification and identification techniques of DSA in Asini Corii Colla are simple, rapid, and high feasibility, which provides a certain reference for the high-value utilization of Asini Corii Colla and the development of functional food.

Indole-3-propionic acid (IPA) has anti-inflammatory properties, which can be beneficial for weaned piglets with underdeveloped immune systems. The study explores the impact of IPA supplementation on growth performance, oxidative stress, and inflammation response in weaned piglets. In Exp. 1, 90 weaned piglets were divided into six groups (5 replicates per group, 3 pigs per replicate), with each group receiving a basal diet with varying amounts of IPA (0, 50, 100, 200, 400, or 600 mg/kg) for 42 d. Piglets fed the diets with 50, 100, and 200 mg/kg of IPA exhibited reduced feed conversion ratios (F:G) compared to the control piglets (P = 0.035). Notably, 50 and 100 mg/kg IPA treatments significantly reduced diarrhea incidence and serum interleukin (IL)-6 content (P

Abstract Background Pathogenic infections can significantly impact the health of livestock. Traditionally, antibiotic growth promoters (AGPs) have been used in feed to enhance growth performance and disease control. However, concerns regarding antibiotic resistance have led to the exploration of traditional herbal medicine as a natural alternative, guided by the principle of medicine-food homology. The Taguchi method was employed to optimize the culture formula for cordycepin production, an active component of Cordyceps militaris (C. militaris). The influences of C. militaris supplementing solid-state fermentation (CMSSF) in feed on the growth performance and immune responses of grower pigs were evaluated in the present study. Results The C. militaris ethanol extract (CME) displayed potent free radical scavenging activity against 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) after undergoing fermentation. Additionally, the antibacterial testing revealed that CME effectively inhibits the growth of common pig pathogens such as Glaesserella parasuis, Pasteurella multocida, Staphylococcus hyicus, and Streptococcus suis. In lipopolysaccharide (LPS)-treated intestinal porcine enterocyte cell line (IPEC-J2), CME significantly suppressed the production of pro-inflammatory cytokines, including tumor necrosis factor (TNF)-α and interleukin (IL)-6. In addition, higher antioxidative activity was detected as indicated by elevated concentration of superoxide dismutase (SOD) in pig serum. The levels of immunoglobulin M (IgM), IgA, and IgG antibodies, as well as classical swine fever virus (CSFV) antibodies (S/P ratio) in serum were all increased. Growth performance of pigs fed with dietary CMSSF supplementation was improved in comparison with the control. Conclusions Results demonstrated that CMSSF has the potential to be used as a natural growth promoter to enhance immunity, antioxidation, as well as overall health and growth performance of grower pigs.

Abstract Fructus Choerospondiatis (FC) has been utilized in Mongolian medicine for the treatment of many diseases. This investigation assessed the coloration, five active components, total phenolic and flavonol contents, as well as the antioxidant and antibacterial properties of different parts of FC from eight cultivars, and the optimal cultivar was identified to determine the anti-inflammatory capacity of all parts. The findings revealed that L* and b* values of the dried peel powder were the highest and the a* value of whole fruit powder was the highest. The concentrations of gallic acid, catechin, epicatechin, total phenols, and total flavonoids exhibited a positive correlation with antioxidant activity. A comprehensive analysis revealed that the peel contained a greater abundance of active compounds and exhibited stronger antioxidant and antibacterial properties compared to other parts. RPNO.1 can be used as the source of high-quality FC for clinical application. In vivo anti-inflammatory experiments indicated that both the flesh and peel of RPNO.1 displayed the highest anti-inflammatory effect. This research not only addressed the gap in studies regarding FC’ s anti-inflammatory properties but also proved that the peel of FC, which was often discarded, had utilization value and could be used as a medicinal herb.

In this study, co-pigmentation with tannin combined with microencapsulation was used to improve the stability of Vitis davidii Foex. anthocyanin. Anthocyanin microcapsules were prepared by vacuum freeze drying using maltodextrin with different dextrose equivalent (DE) values as the wall material. The results showed that the co-pigmented anthocyanin microcapsules with maltodextrin DE12 had the highest encapsulation efficiency of (99.23 ± 0.31)% and the best hygroscopicity and thermal stability. Scanning electron microscopy (SEM) showed that the surface morphology of all four microcapsules was glassy structure. The co-pigmented anthocyanin microcapsules had higher L* and a* values but lower b* value than the unco-pigmented ones. Differential scanning calorimetry (DSC) analysis showed that the co-pigmented anthocyanin microcapsules with maltodextrin DE12 had higher thermal denaturation temperature and thermal stability. Fourier transform infrared spectroscopy (FTIR) confirmed successful microencapsulation of co-pigmented anthocyanin with maltodextrin. The co-pigmented anthocyanin microcapsules with maltodextrin DE12 had good sustained release performance and strong antioxidant capacity. After simulated gastric digestion, the percentage of anthocyanins released from the microcapsules was only (56.84 ± 0.02)%. After simulated intestinal digestion, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the microcapsules was (50.15 ± 0.38)%. Moreover, the degradation kinetics of the microcapsules followed first-order kinetics model and the microcapsules had good storage stability. Microencapsulation could effectively reduce the loss of anthocyanins during the pasteurization of V. davidii Foex. grape juice.

Abstract This research aimed to investigate the effects of dietary fructooligosaccharides (FOS) on attenuating the Aeromonas hydrophila (A. hydrophila)-induced oxidative stress and apoptosis in blunt snout bream Megalobrama amblycephala. Fish were divided into three groups as follows: C1 (Control), T1 (A. hydrophila), and T2 (A. hydrophila + 4 g/kg FOS). The results showed that the activities of antioxidant enzymes increased, the liver morphology had disorderly arrangement, and extensive cell necrosis occurred because of A. hydrophila-infection. While the dietary FOS improved the above-mentioned liver damage. Additionaly, FOS elevated mRNA levels of pro-apoptotic molecules, including caspase-8 and 9, and down-regulated mRNA levels of the anti-apoptotic molecule Bcl-2, which is triggered by A. hydrophila-infection. The transcriptome analysis showed that the oxidative stress-related DEGs pathways were activated in intestine of blunt snout bream by A. hydrophila-infection. The FOS-added group led to the enrichment of more pathways to health. Further WGCNA co-expression network analysis showed that the screened single genes were clustered into 49 modules. The two modules with the highest association to the five traits (10 hub genes) were chosen to build the network by combining the physiological and biochemical characteristic. In summary, this research offers a foundation for the exploring of A. hydrophila-restoration genes in dietary FOS, and also lays a theoretical foundation for aquaculture in the future.

This study was designed to explore the hepatoprotective effects of enzymatic degradation products derived from sturgeon roe on alcohol-damaged hepatocytes and to elucidate the underlying mechanisms. Utilizing sturgeon roe as the research material, the enzymatic process was optimized by focusing on key parameters such as enzymatic efficiency and in vitro activity. The protective effects on hepatocytes were systematically investigated by using the HepG2 cell model. Advanced computerized virtual screening technology was employed to identify potential bioactive peptides within the sturgeon roe. The findings indicated that the optimum enzymatic product of sturgeon roe was obtained under the conditions of 1:1 compounding of alkaline protease (1343 U/g) and trypsin (27 U/g) for 8 h, yielded a protein recovery of 49.30%±0.57%, a hydrolysis degree of 46.05%±0.92%, and ABTS+ free radical scavenging rate of 51.45%±0.66%. Notably, at a concentration of 5 mg/mL, this product significantly enhanced the activity of alcohol dehydrogenase (ADH) by 168.64%±1.42% (P

BACKGROUND: During healing process of chronic wounds, excessive production of reactive oxygen species can impair the function of L929 fibroblasts, thereby delaying wound repair. Therefore, protecting fibroblasts from oxidative stress is important to promote wound healing. OBJECTIVE: To assess the protective effects of carboxymethyl chitosan-oxidized chondroitin sulfate/platelet-rich plasma (CMC-OCS/PRP) hydrogel on L929 cells under H2O2 stimulation. METHODS: CMC-OCS/PRP hydrogels were prepared, and the micromorphology, degradation performance, scavenging ability of H2O2 and hydroxyl radical and biocompatibility of the hydrogels were characterized. L929 cells with good growth state were taken and cultured in five groups. The control group was cultured conventionally. H2O2 was added to the H2O2 group. Carboxymethyl chitosan-oxidized chondroitin sulfate hydrogel extract + H2O2 was added to the CMC- OCS group. Platelet-rich plasma gel extract + H2O2 was added to the PRP group. The CMC-OCS/PRP group was treated with carboxymethyl chitosan-oxidized chondroitin sulfate/platelet-rich plasma hydrogel extract + H2O2. Each group was treated with hydrogel extract for 6 hours, and then H2O2 for 24 hours. After culture, the levels of active oxygen and malondialdehyde, apoptosis and expression of collagen fiber I protein were detected. In the presence of H2O2, the above hydrogel extracts were directly or indirectly co-cultured with L929 fibroblasts for 36 hours, respectively. Migration ability of the cells was detected by scratch test and Transwell chamber test. RESULTS AND CONCLUSION: (1) CMC-OCS/PRP hydrogels had uniform and interrelated porous structure and good degradation ability, could effectively remove H2O2 and hydroxyl radicals in vitro, and had good biocompatibility. (2) Compared with the control group, the apoptosis rate, reactive oxygen species, and malondialdehyde levels were increased (P < 0.05); the spread area of cells was decreased (P < 0.05), and the expression of collagen fiber I protein had no significant changes (P > 0.05) in the H2O2 group. Compared with the H2O2 group, reactive oxygen species level was decreased in the CMC-OCS group (P < 0.05), malondialdehyde level was decreased (P < 0.05), and cell spread area was increased (P <0.05) in the PRP group, CMC-OCS group, and CMC-OCS/PRP group; apoptosis rate was decreased in the CMC-OCS/PRP group (P < 0.05), and collagen fiber I protein expression was increased in the PRP group, CMC-OCS group, and CMC-OCS/PRP group (P < 0.05). (3) Compared with the control group, the number of cell migration was decreased (P < 0.05), and the migration area had no significant change (P > 0.05) in the H2O2 group. Compared with the H2O2 group, the number and area of cell migration were increased in the PRP group, CMC-OCS group, and CMC-OCS/PRP group (P < 0.05), and the increase was most significant in the CMC-OCS/PRP group. (4) Under oxidative stress, CMC-OCS/ PRP hydrogel can improve the migration ability of fibroblasts, resist cell apoptosis, and preserve cell extension function [ABSTRACT FROM AUTHOR]

Goji (Lycium barbarum L.) leaves flavonoid microcapsules (M-LBLF) were prepared using gelatin and carboxymethyl cellulose sodium (CMC) as wall materials, and the stability and biological activities were investigated. The preparation process of M-LBLF was optimized through response surface methodology. The stability of the product was detected using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), thermogravimetric analysis and in vitro simulated digestion. The biological activity of the product was evaluated through free radical scavenging and enzyme activity inhibition experiments. Results showed that, the optimal process conditions for M-LBLF were a core to wall ratio of 1:3.86, a wall material concentration of 1.15%, a stirring temperature of 45 ℃, and an embedding rate of 84.21%. SEM observation showed that most of the surface of the microcapsule particles was smooth, the structure of the capsule wall remained intact, and there were no signs of rupture on the wall material. FT-IR spectra showed a shift in C=O at 1594 cm−1 in Lycium barbarum leaves flavonoids (LBLF), suggesting an electrostatic interaction between LBLF and CMC. XRD spectra showed that due to microencapsulation, the amorphous structure of the product was partially transformed into a crystalline state, resulting in slight changes in the diffraction peak intensity of M-LBLF and LBLF. Thermogravimetric analysis found that microencapsulation improved the high-temperature decomposition, playing a protective role for LBLF. In vitro simulated digestion and antioxidant activity experiments showed that microencapsulation improved the stability of LBLF and retained good antioxidant activity during digestion. In addition, the equations were fitted by multiple linear regression to obtain the semi-inhibitory mass concentration (IC50) of the microcapsules against pancreatic lipase (2.2004±0.03 mg/mL) and against α-amylase (2.188±0.02 mg/mL), suggesting that M-LBLF was able to inhibit both pancreatic lipase activity and α-amylase activity, which suggested that it had the potential to regulate the glycolipid metabolism activity. This research would provide a foundation for improving the stability of flavonoids extracted from goji leaves and developing the related nutritional and health foods.

The demand for beneficial, safe natural pigments is growing. Clitoria ternatea (CT) flowers are a promising source of natural blue colours and bioactive compounds. This study extracted intact CT flowers (CTFs) and their powders (CTFPs) with distilled water at various temperatures (30-90 °C) and durations (30-180 min). The total anthocyanin content (TAC) and total phenolic content (TPC) of the CT extracts were analysed by using the pH differential and Folin-Ciocalteu methods, respectively. The antioxidant activity was expressed as the ability to scavenge DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals. The results showed that different sample types significantly (P

Polyacrylonitrile pre-oxidized fiber（PANOF）is an emerging flame retardant material with low cost and high performance，showing a promising application potential as thermal protection materials in the aerospace field.Pretreated PANOF（t-PANOF）was obtained by the process of oil removing with acetone and surface modification using silane coupling agent KH570 in turn.Scanning electron microscopy（SEM），Fourier transform infrared spectroscopy（FT-IR）and X-ray photoelectron spectroscopy（XPS）were employed to figure out the changes of morphology and chemical structure of the fibers before and after pretreatment and their interfacial properties with silicone rubber（SR）.Effects of t-PANOF on the mechanical and thermal performances of SR were further investigated. With the presence of ceramic forming agent（CFA），the ablation responsive behaviors of CFA/t-PANOF/SR ternary composites were researched as well.The results show that the level of interfacial adhesion between fibers and rubber is significantly enhanced contributed from the pretreatment of PANOF，and the tensile strength of the composites is improved accordingly.However，with the increasing content of t-PANOF，the comprehensive mechanical properties of t-PANOF/SR composites show the trend that increase first and then decrease，indicating that the optimal dosage of the fibers is 5 to 10 phr.t-PANOF skeleton helps to not only enhance the dimensional stability of SR in high temperature and oxygen-rich environment，but also improve the ablation performance of CFA/t-PANOF/SR composites.The antioxidation ceramic layer with fiber skeleton as the main structure is formed on the surface of CFA/t-PANOF/SR composites under the high temperature aerobic gas with the heat flux density of 2 MW/m2，the corresponding linear and mass ablation rate are only 0.02 mm/s and 0.02 g/s respectively，which is expected to be utilized as an ablation material to satisfy the demands of the new generation of aerospace vehicles.

Introduction: The formation of advanced glycation end products (AGEs) due to prolonged high blood sugar levels and oxidative stress is crucial for accelerating several chronic diseases, including diabetic complications. Edible Thai plants, such as Ficus botryocarpa (FB) and F. racemosa (FR), are abundant in phytochemicals and exhibit antioxidant, antidiabetic, and antimicrobial properties. This study aimed to evaluate the potential effects of ethanolic extracts derived from the fruits of FB and FR (referred to as FBE and FRE, respectively) on glycation and oxidative stress in vitro. In addition, the total phenolic and flavonoid compounds in the extracts were measured. Methods: Phenolic and flavonoid contents were determined using the Folin-Ciocalteu and aluminium chloride methods, respectively. To evaluate their antiglycation capabilities, the extracts were tested along with aminoguanidine as a positive control using a fructose-induced bovine serum albumin (BSA) glycation model. Fluorescent AGE and fructosamine levels were quantified. The inhibition of lipid peroxidation and superoxide anion radicals was evaluated using thiobarbituric acid-reactive substances (TBARS) and a riboflavin–light–nitroblue tetrazolium assay, respectively. Results: The phenolic and flavonoid compounds in FBE extract were greater than those in the FRE. FBE inhibited AGEs and produced a significant reduction in fructosamine levels more effectively than FRE (P

Silver nanoparticles (AgNPs) are widely used in the field of biomedicine. However, the synthesis of AgNPs by traditional physical and chemical methods has problems such as pollution, product toxicity, and time-consuming. Therefore, it is extremely important to explore the preparation of AgNPs. Silver nanoparticles (AgNPs) of sweet corncob polysaccharide (SCP-AgNPs) were successfully synthesized by green method using sweet corncob polysaccharide (SCP) as raw material and the reaction conditions were optimized. The generated AgNPs were characterized by transmission electron microscopy (TEM), X-ray diffraction, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy, and their antioxidant and anti-diabetic abilities in vitro were investigated. The results showed that the optimum reaction conditions of SCP-AgNPs were as follows: The concentration of silver nitrate in the solution was 0.75×10−3 mol/L, the pH was 10.0, and the reaction time was 80 min. The prepared SCP-AgNPs were spherical with an average particle size of 13.5 nm. The structure showed a face-centered cubic crystal structure, and Ag was determined to be Ag0 state. The synthesized SCP-AgNPs had strong free radical scavenging effect in vitro. At the same time, SCP-AgNPs had a good inhibitory effect on the activity of α-glucosidase and α-amylase. SCP-AgNPs has a significant inhibitory effect on key enzymes of diabetes and has potential anti-diabetic activity. It is a nano-drug suitable for nano-biomedical applications and will be widely used in food processing, medicine and other fields in the future.

Epilepsy, a chronic neurological disorder affecting millions globally, is often exacerbated by neuroinflammation and oxidative stress. Existing antiepileptic drugs primarily manage symptoms, leaving the disease's progression largely unaddressed. Flavonoids, ubiquitous plant metabolites with potent antiinflammatory and antioxidant properties, show promise in epilepsy treatment. Unlike conventional therapies, they targetmultiple pathophysiological processes simultaneously, offering a comprehensive approach to this complex neurological disorder. This review explores the dual role of flavonoids in mitigating neuroinflammation and reducing oxidative stress through various molecular pathways. By inhibiting key inflammatory mediators and pathways such as NF-κB, MAPK, JNK, and JAK, flavonoids offer neuronal protection. They enhance the body's natural antioxidant defenses by modulating enzyme activities, including superoxide dismutase, catalase, and glutathione peroxidase. Moreover, flavonoids influence crucial antioxidant response pathways like PI3K/AKT, Nrf2, JNK, and PKA. Despite their therapeutic promise, the low bioavailability of flavonoids poses a considerable challenge. However, cutting-edge strategies, including nanotechnology and chemical modifications, are underway to improve their bioavailability and therapeutic efficacy. These advancements support the potential of flavonoids as a valuable addition to epilepsy treatment strategies. [ABSTRACT FROM AUTHOR]

Animal coloration serves a variety of visually related functions in nature (e.g. mate choice, aposematism and camouflage) but the pigments in integumentary tissues such as skin, scales and feathers may also serve functions unrelated to the visual environment (e.g. temperature regulation, detoxification and pollutant protection). Our understanding of the significance of the non‐visual functions of animal integumentary pigments, as well as how they interact with the visually related functions to shape animal visual systems, remains limited. Furthermore, due to their important roles in shaping species interactions and mediating interactions in the environment, animal colour traits are likely to be impacted by global change (e.g. increased temperatures, altered habitat quality and quantity, increased environmental stochasticity, pollutants and novel species assemblages). Considering the effects of global change on both visual and non‐visual functions is important for understanding whether the selection is acting directly on the pigment or on coloration. Since changing the trait distributions can then lead to changes in visual systems, we advocate for studies to consider all potential functions of integumentary pigments, both visual and non‐visual functions and their interaction. Towards this goal, we first highlight common functions of pigments with a focus on non‐visual functions across animal systems. Then we synthesize our current understanding of how global change can impact pigmentation and discuss factors that can modify the interactions between climate change and pigment function. Lastly, we discuss how changes in colour traits can impact visual systems and provide an example using amphibians and their responses to climate change as a model. Read the free Plain Language Summary for this article on the Journal blog. [ABSTRACT FROM AUTHOR]

Introduction: Inonotus hispidus, commonly referred to as the Sanghuang mushroom, is a species that is consumed as a tea. To date, this is the only species of the same fungus that has been successfully cultivated. Methods: A single-factor test was conducted using Inonotus hispidus MS-5 and MS-9 as test materials. The response surface methodology was adopted to design and optimise the liquid fermentation medium for them. Results: As indicated in the results, the optimum fermentation conditions for MS-5 include 24.09g/L glucose, 7.88g/L yeast extract, 0.99g/L dandelion powder, 1.5g MgSO4, 2g KH2PO4, 0.01g vitamin B1, and 1L deionized water; the optimum fermentation conditions for MS-9 include 24.64g/L glucose, 7.77g/L yeast extract, 0.98g/L dandelion powder, 1.5g MgSO4, 2g KH2PO4, 0.01g vitamin B1, and 1L deionized water. Under such conditions, the mycelial biomass (dry weight) values were able to reach 16.02g/L and 14.91g/L for MS-5 and MS-9, respectively, which were 1.6 and 1.54 times those measured before optimization. Discussion: As revealed in the antioxidant and anticancer experiment, Inonotus hispidus exopolysaccharides has corresponding functional effects at the cellular level. This research optimised the liquid culture formulation of Inonotus hispidus and demonstrated that the function of it as a traditional Sanghuang herbal tea is well-documented. [ABSTRACT FROM AUTHOR]

Melatonin (N-acetyl-5-methoxytryptamine) is an essential small molecule with diverse biological functions. It plays several key roles, including regulating the secretion of reproductive hormones and the reproductive cycle, enhancing the functionality of reproductive organs, improving the quality of sperm and eggs, and mitigating oxidative stress in the reproductive system. Melatonin effectively inhibits and scavenges excess free radicals while activating the antioxidant enzyme system and reduces the production of inflammatory factors and alleviates tissue damage caused by inflammation by regulating inflammatory pathways. Additionally, melatonin contributes to repairing the intestinal barrier and regulating the gut microbiota, thereby reducing bacterial and toxin permeation. The use of melatonin as an endogenous hormone in animal husbandry has garnered considerable attention because of its positive effects on animal production performance, reproductive outcomes, stress adaptation, disease treatment, and environmental sustainability. This review explores the characteristics and biological functions of melatonin, along with its current applications in animal production. Our findings may serve as a reference for the use of melatonin in animal farming and future developmental directions. [ABSTRACT FROM AUTHOR]

In order to utilize Radix isatidis residue in high value, the solid fermentation process from Radix isatidis residue to produce polysaccharide feed additive was optimized, and its antioxidant capacity was studied. The results showed that the high-yielding cellulase strain Bacillus subtilis YBS-18 was isolated from 28 strains. The production process of polysaccharide from solid fermentation of Bacillus subtilis YBS-18 isatis root residue was optimized by single factor test and response surface. The optimal process was determined as follows: The liquid-solid ratio was 1.2 mL/g, the loading rate was 0.3 g/mL, the glucose concentration was 8%, the ammonium sulfate concentration was 4.5%, the inoculation rate was 4.5%, and the fermentation temperature was 38 °C. Under the optimum fermentation conditions, the polysaccharide content reached 28.17 mg/g. The polysaccharide showed good scavenging ability for DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals, and the scavenging rate increased with the increase of polysaccharide concentration. The results show that the process improved the nutritional value of Radix isatidis residue and provided a theoretical basis for the development and application of feed additives of Radix isatidis residue. [ABSTRACT FROM AUTHOR]

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The experiment aimed to optimize the extraction process of Euonymus fortunei (Turcz.) Hand.-Mazz. flavonoids and study their anti-fatigue and antioxidant effects. The experiment took the extraction rate of Euonymus fortunei (Turcz.) Hand.-Mazz. flavonoids as the index and conducted single-factor experiments on factors such as liquid-material ratio, ethanol concentration, extraction time, and ultrasonic power. Based on this, a response surface optimization design was carried out to investigate the anti-fatigue effect of Euonymus fortunei (Turcz.) Hand.-Mazz. Fifty mice were randomly divided into a blank control group, a positive control group (Euonymus fortunei (Turcz.) Hand.-Mazz. compound mixture), a fatigue model group, and low and high dose groups of Euonymus fortunei (Turcz.) Hand.-Mazz. flavonoid extract (200 mg/kg and 400 mg/kg), with 10 mice in each group, for the anti-fatigue experiment in mice. The results showed that the optimal extraction conditions for Euonymus fortunei (Turcz.) Hand.-Mazz. flavonoids had a liquid-material ratio of 55 mL/g, an ethanol concentration of 60%, an extraction time of 73 min, and an ultrasonic power of 175 W, under which the flavonoid yield was 5.94%, which was basically consistent with the predicted value. Euonymus fortunei (Turcz.) Hand.-Mazz. flavonoids could effectively extend the swimming time of mice carrying weights, reduce the concentrations of lactic acid (LD) and blood urea nitrogen (BUN) in serum, increase the levels of liver glycogen and muscle glycogen in mice, enhance the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and reduce the content of malondialdehyde (MDA). The study shows that Euonymus fortunei (Turcz.) Hand.-Mazz. flavonoids have good anti-fatigue and antioxidant effects and can be used as a feed additive to alleviate animal fatigue and oxidative stress. [ABSTRACT FROM AUTHOR]

The purpose of this study was to optimize the extraction process of polysaccharides from Shang Platycodon and to analyze its hygroscopic, moisturizing and antioxidant properties. Orthogonal experiments were used to optimize the extraction process of polysaccharides from Shang Platycodon by ultrasonic-assisted water extraction and alcohol precipitation. The polysaccharides extracted under the optimal extraction process were refined and used for the determination and analysis of hygroscopic moisturizing and antioxidant properties. The results showed that the extraction rate of polysaccharides was 9.75% when the liquid-material ratio was 20 mL/g, the ultrasonic extraction temperature was 50 ℃, and the ultrasonic extraction time was 20 min, which was the best extraction process for polysaccharides from Shang Platycodon. In the case of RH 60% and RH 81%, the order of moisture absorption rate was glycerol > sodium alginate > glycerol polysaccharides; and in dry envi⁃ronment, the moisture retention rate was sodium alginate > polysaccharides of bellflower > glycerol. Polysaccharides from Shang Platycodon can scavenge hydroxyl radicals and DPPH free radicals, and the concentration of polysaccharides is positively correlated with scavenging ability. Among them, when the concentrations of polysaccharides were 2.0 mg/mL and 10.0 mg/mL, respectively, half of the hydroxyl radicals and DPPH radicals could be inhibited. Studies have shown that the polysaccharide from Shang Platycodon is not enough hygroscopic, but it has good moisturizing property and has certain antioxidant properties, which can be developed as a natural animal feed additive. [ABSTRACT FROM AUTHOR]