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Laboratory studies were conducted to standardise optimum dose of gamma irradiation to induce sterility in males of South American tomato moth [Phthorimaea (Tuta) absoluta (Meyrick)], a major pest on tomato. Among the different irradiation doses tested, 150 Gy was found to be effective to induce male sterility in P. absoluta, where sterile males exhibited mating competitiveness, significantly reduced fecundity and hatchability in F1 and F2 generations. Five days old pupae were found ideal for exposing to gamma irradiation to induce male sterility in P. absoluta that could be used for area wide management using SIT.