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Abstract: Light variation in temporal and spatial domains is a key constraint on the photosynthetic performance of phytoplankton. The most obvious responses are the modification of cell pigment content either to improve photocapture or to mitigate photo-damage. Very few studies have analyzed whether light variation significantly alters carbon assimilation, especially in a fluctuating light environment as in the mixed layer of the ocean. We addressed the question using a modeling approach, which allows the reproduction of most of the possible scenarios, obtained with great difficulty from laboratory or field experiments. The complete model is based on the dynamic coupling of a photoacclimation and photodamage-repair responses. In this combined model the virtual phytoplankton is exposed to different light regimes (steady, square wave, sinusoidal light–dark cycles and fluctuating regimes). The results reconcile controversial results on different photacclimation states achieved during fluctuating light regimes. The model produces a depression of carbon assimilation in any light fluctuating scenario, as compared to steady light regimes, due to the temporal delay between light fluctuations and photoresponses. These results suggest the possibility of selective pressure during evolution, more effective on photoprotective mechanisms than on optimization of light harvesting. [Copyright &y& Elsevier]

Abstract: We attempt to tackle the problem of the direct numerical simulation of the chemical corrosion of iron through the solution of an evolutive system of partial differential equations based on the electrochemistry laws. The process is described in terms of the concentrations of the chemical species appearing within the chemical reactions and the electric potential. In fact in the literature corrosion processes have been object of microscopic descriptions built upon ‘ab initio’ molecular dynamics and of phenomenological models based on data fitting techniques. In the first case, the simulation procedures are too lengthy and not suitable for applicative purposes, whereas in the second case, the validity of the models is obviously restricted to the experimental set-up. Interesting, but necessarily a posteriori, descriptions have been developed based on inverse modeling. Here we present the simulation of the primary reactions occurring during the corrosion of the irony bottom of a closed vertical slot filled with an acidic solution and whose top and bottom, which are connected by an external conducing wire, work respectively as a cathode and an anode of a galvanic cell. The curves of the evolution of the concentration of the ions, of the pH of the solution and of the electric potential are discussed. [Copyright &y& Elsevier]

We present new important results about the intermediate-type Seyfert galaxy Mrk 315, recently observed through optical imaging and integral-field spectroscopy. Broad-band images were used to study the morphology of the host galaxy, narrow-band Hα images to trace the star-forming regions, and middle-band[O iii] images to evidence the distribution of the highly ionized gas. Some extended emission regions were isolated and their physical properties studied by means of flux-calibrated spectra. High-resolution spectroscopy was used to separate different kinematic components in the velocity fields of gas and stars. Some peculiar features characterize this apparently undisturbed and moderately isolated active galaxy. Such features, already investigated by other authors, are re-analysed and discussed in the light of these new observations. The most relevant results we obtained are: the multitiers structure of the disc; the presence of a quasi-ring of regions with star formation much higher than previous claims; a secondary nucleus confirmed by a stellar component kinematically decoupled by the main galaxy; a new hypothesis about the controversial nature of the long filament, initially described as hook shaped, and more likely made of two independent filaments caused by interaction events between the main galaxy and two dwarf companions. [ABSTRACT FROM AUTHOR]

Several authors have recently explored, for narrow-line Seyfert 1 galaxies (NLS1s), the relationship between black hole mass (MBH) and stellar velocity dispersion (σ*). Their results are more or less in agreement and seem to indicate that NLS1s fill the region below the fit obtained by Tremaine et al., showing a range ofσ* similar to that of Seyfert 1 galaxies, and a lowerMBH. Until now, the[O iii] width has been used in place of the stellar velocity dispersion, but some indications have begun to arise against the effectiveness of the gaseous kinematics in representing the bulge potential, at least in NLS1s. Bian&Zhao have stressed the urgency of producing trueσ* measurements. Here, we present new stellar velocity dispersions obtained through direct measurements of the Ca iiabsorption triplet (∼8550Å) in the nuclei of eight NLS1 galaxies. The resultingσ* values and a comparison withσ[O III] confirm our suspicion that[O iii] typically overestimates the stellar velocity dispersion. We demonstrate that NLS1s follow therelation as Seyfert 1, quasars and non-active galaxies. [ABSTRACT FROM AUTHOR]

Measuring stellar velocity dispersion (Ã) in Narrow-Line Seyfert 1 (NLS1) with optical spectra, can be very difficult because of the large and bright Fe II multiplets, which completely suppress the typically used stellar absorption lines. Indeed, à values are to date available only for 2 NLS1s. Here we present new à measurements of three NLS1s observed in the spectral range centered around the Ca II triplet (»<8600Å). Moreover, we compare à with the gaseous velocity dispersion finding that the à = FWHM([O III]5007Å)/2.35 relation (Nelson & Whittle 1995) is not satisfied by our galaxies.To search for other articles by the author(s) go to: http://adsabs.harvard.edu/abstract_service.html [ABSTRACT FROM PUBLISHER]

Arginine-vasotocine (AVT) injected into male adult lizards, Podarcis s. sicula Raf., inhibits the last phases of spermatogenesis and the endocrine activity of gonads. In female lizards the substance induces an early interruption of egg deposition. [ABSTRACT FROM AUTHOR]

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Marine biofouling is considered one of the major biophysical processes influencing the vertical dynamics of plastic debris in seawater. We numerically implement, for the first time, this mechanism within a fine-resolution, regional model of the Tyrrhenian Sea, in order to simulate the dispersion of microplastics (MPs) released at the mouth of a highly polluting river. Four polymers and three particle sizes are used to quantify algal concentration influence on the trajectories, fates, and accumulation spots of the tracked MPs, by comparing 2002 winter and summer runs encompassing or not biofouling. Besides a marked seasonality for most of the MP types and radii tested, biofouling effects are prominently observed for only 2 polymers and particles bigger than 1μm. Thus, further realistic applications of the biofouling mechanism in oceanic circulation models are required to achieve a thorough assessment of its impact on plastic density within distinctive basins of the world seas., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Environmental DNA metabarcoding (eDNA metaB) is fundamental for monitoring marine biodiversity and its spread in coastal ecosystems. We applied eDNA metaB to seawater samples to investigate the spatiotemporal variability of plankton and small pelagic fish, comparing sites with different environmental conditions across a coast-to-offshore gradient at river mouths along the Campania coast (Italy) over 2 years (2020-2021). We found a marked seasonality in the planktonic community at the regional scale, likely owing to the hydrodynamic connection among sampling sites, which was derived from numerical simulations. Nonetheless, spatial variability among plankton communities was detected during summer. Overall, slight changes in plankton and fish composition resulted in the potential reorganization of the pelagic food web at the local scale. This work supports the utility of eDNA metaB in combination with hydrodynamic modelling to study marine biodiversity in the water column of coastal systems. This article is part of the theme issue 'Connected interactions: enriching food web research by spatial and social interactions'.

Anchovies represent the largest world's marine fish catches and the current threats on their populations impose a sustainable exploitment based on sound scientific information. In the European anchovy (Engraulis encrasicolus), the existence of several populations has been proposed but a global view is missing. Using a multidisciplinary approach, here we assessed the divergence among different ecotypes and its possible causes. SNPs have revealed two functionally distinct ecotypes overlapping in the Central Mediterranean, with one ecotype confined near the river estuaries. The same SNPs outliers also segregated two distinct populations in the near Atlantic, despite their large spatial distance. In addition, while most studies suggested that adaptation to low salinity is key to divergence, here we show that the offshore ecotype has higher environmental tolerance and an opportunistic feeding behaviour, as assessed by the study of environmental conditions, anchovy diet and trophic levels, and passive egg dispersal. These results provide insights into the anchovy evolutionary history, stressing the importance of behaviour in shaping ecotypes.

Planktonic copepods display a large repertoire of motion behaviors in a three-dimensional environment. Two-dimensional video observations demonstrated that the small copepod Clausocalanus furcatus, one the most widely distributed calanoids at low to medium latitudes, presented a unique swimming behavior that was continuous and fast and followed notably convoluted trajectories. Furthermore, previous observations indicated that the motion of C. furcatus resembled a random process. We characterized the swimming behavior of this species in three-dimensional space using a video system equipped with telecentric lenses, which allow tracking of zooplankton without the distortion errors inherent in common lenses. Our observations revealed unexpected regularities in the behavior of C. furcatus that appear primarily in the horizontal plane and could not have been identified in previous observations based on lateral views. Our results indicate that the swimming behavior of C. furcatus is based on a limited repertoire of basic kinematic modules but exhibits greater plasticity than previously thought.

Unlabelled: D-Aspartic acid (D-Asp) and nitric oxide (NO) play an important role in tuning testosterone production in the gonads of male vertebrates. In particular, D-Asp promotes either the synthesis or the release of testosterone, whereas NO inhibits it. In this study, we have investigated for the first time in birds the putative effects of D-Asp and NO on testicular testosterone production in relation to two phases of the reproductive cycle of the adult captive wild-strain mallard (Anas platyrhynchos) drake. It is a typical seasonal breeder and its cycle consists of a short reproductive period (RP) in the spring (April-May) and a non reproductive period (NRP) in the summer (July), a time when the gonads are quiescent. The presence and the localization of D-Asp and NO in the testis and the trends of D-Asp, NO and testosterone levels were assessed during the main phases of the bird's reproductive cycle. Furthermore, in vitro experiments revealed the direct effect of exogenously administered D-Asp and NO on testosterone steroidogenesis., Methods: By using immunohistochemical (IHC) techniques, we studied the presence and the distributional pattern of D-Asp and NO in the testes of RP and NRP drakes. D-Asp levels were evaluated by an enzymatic method, whereas NO content, via nitrite, was assessed using biochemical measurements. Finally, immunoenzymatic techniques determined testicular testosterone levels., Results: IHC analyses revealed the presence of D-Asp and NO in Leydig cells. The distributional pattern of both molecules was in some way correlated to the steroidogenic pathway, which is involved in autocrine testosterone production. Indeed, whereas NO was present only during the NRP, D-Asp was almost exclusively present during the RP. Consistently, the high testosterone testicular content occurring during RP was coupled to a high D-Asp level and a low NO content in the gonad. By contrast, in sexually inactive drakes (NRP), the low testosterone content in the gonad was coupled to a low D-Asp content and to a relatively high NO level. Consequently, to determine the exogenous effects of the two amino acids on testosterone synthesis, we carried out in vitro experiments using testis sections deriving from both the RP and NRP. When testis slices were incubated for 60 or 120 min with D-Asp, testosterone was enhanced, whereas in the presence of L-Arg, a precursor of NO, it was inhibited., Conclusion: Our results provide new insights into the involvement of D-Asp and NO in testicular testosterone production in the adult captive wild-strain mallard drake. The localization of these two molecules in the Leydig cells in different periods of the reproductive cycle demonstrates that they play a potential role in regulating local testosterone production.

D-Aspartic acid (D-Asp) and nitric oxide (NO) are two biologically active molecules playing important functions as neurotransmitters and neuromodulators of nerve impulse and as regulators of hormone production by endocrine organs. We studied the occurrence of D-Asp and NO as well as their effects on testosterone synthesis in the testis of boar. This model was chosen for our investigations because it contains more Leydig cells than other mammals. Indirect immunofluorescence applied to cryostat sections was used to evaluate the co-localization of D-Asp and of the enzyme nitric oxide synthase (NOS) in the same Leydig cells. D-Asp and NOS often co-existed in the same Leydig cells and were found, separately, in many other testicular cytotypes. D-Asp level was dosed by an enzymatic method performed on boar testis extracts and was 40+/-3.6 nmol/g of fresh tissue. NO measurement was carried out using a biochemical method by NOS activity determination and expressed as quantity of nitrites produced: it was 155.25+/-21.9 nmol/mg of tissue. The effects of the two molecules on steroid hormone production were evaluated by incubating testis homogenates, respectively with or without D-Asp and/or the NO-donor L-arginine (L-Arg). After incubation, the testosterone presence was measured by immunoenzymatic assay (EIA). These in vitro experiments showed that the addition of D-Asp to incubated testicular homogenates significantly increased testosterone concentration, whereas the addition of L-Arg decreased the hormone production. Moreover, the inclusion of L-Arg to an incubation medium of testicular homogenates with added D-Asp, completely inhibited the stimulating effects of this enantiomer. Our results suggest an autocrine action of both D-Asp and NO on the steroidogenetic activity of the Leydig cell.

This study investigated the involvement of D-aspartic acid (D-Asp) in testicular steroidogenesis of the green frog Rana esculenta and its effect on stimulation of thumb pad morphology and glandular activity, a typical testosterone-dependent secondary sexual characteristic in this amphibian species. In the testis, D-Asp concentrations vary significantly during the reproductive cycle: they are low in pre- and post-reproductive periods, but reach peak levels in the reproductive period (140-236 nmol/g wet tissue). Moreover, the concentrations of D-Asp in the testis through the sexual cycle positively match the testosterone levels in the gonad and the plasma. The racemase activity evaluated during the cycle expresses its peak when D-Asp and testosterone levels are highest, that is, during the reproductive period, confirming the synthesis of D-Asp from L-Asp by an aspartate racemase. Short-term in vivo experiments consisting of a single injection of D-Asp (2.0 micro mol/g body weight) demonstrated that this amino acid accumulates significantly in the testis, and after 3 h its uptake is coupled with a testosterone increase in both testis and plasma. Moreover, within 18 h of amino acid administration, the D-Asp concentration in the testis decreased along with the testosterone titer to prestimulation levels. Other amino acids (L-Asp, D-Glu and L-Glu) used instead of D-Asp were ineffective, confirming that the significant increase in testicular testosterone was a specific feature of this amino acid. In long-term experiments, D-Asp had been administered chronically to frogs caught during the three phases of the reproductive cycle, inducing testosterone increase and 17beta-estradiol decrease in the gonad during the pre- and post-reproductive period, and vice versa during the reproductive period. The stimulatory effect of D-Asp on testosterone production by the testis is consistent with the stimulation of spermatogenesis and the maturation of thumb pads occurring in D-Asp-treated frogs. In these last animals, there was an increase of seminiferous ampoule area and a higher number of spermatids and sperm. Moreover, in spermatogonia I and II and in spermatocytes, a proliferating cell nuclear antigen (PCNA) intense immunopositivity was observed. In addition, the thumb pads of D-Asp-treated frogs compared with controls showed a significantly thicker epithelial lining, a wider area of their glands with taller secretion cells, and more numerous, PAS-positive-rich secretions. Finally, these results provide functional evidence for a biologic role of D-Asp in amphibian male steroidogenesis; therefore, this unusual amino acid could be considered a modulatory agent for reproductive processes.

The distribution of nicotinamide adenine dinucleotide phosphate reduced diaphorase (NADPH-d) containing neurons was examined in the oviduct of the lizard Podarcis s. sicula and the relationship between these neurons and 17beta-estradiol hormone was studied. NADPH-d-histochemistry and indirect immunofluorescence method were applied to cryostat sections. NADPH-d-nerve structures were found throughout the oviduct. Positive neurons were primarily located in the reproductive oviduct, and were more numerous in the intermuscular and circular muscle layers than in the mucosa. The vagina revealed a reactive nerve population denser than elsewhere. The NADPH-d-positive neurons densities and the 17beta-estradiol plasma levels coincided throughout the lizard sexual cycle. In addition, after 17beta-estradiol treatments, non-reproductive lizards showed an increase of NADPH-d neurons. We suppose that nitric oxide (NO) neurons play an estrogen-dependent role in the oviduct muscle motility.

The present study investigated the role of D-aspartic acid (D-Asp) in ovarian steroidogenesis and its effect on aromatase activity in the lizard, Podarcis s. sicula. It was determined that D-Asp concentrations vary significantly during phases of the reproductive cycle: they vary inversely with testosterone concentrations and directly with oestradiol concentrations in the ovary and plasma. Experimental treatment showed that administration of D-Asp induces a decrease in testosterone and an increase in oestradiol, and that treatment with other amino acids (L-Asp, D-Glu and D-Ala) instead of D-Asp has no effects. Experiments in vitro confirmed these results. Furthermore, these experiments showed an increase in aromatase activity, as the addition of D-Asp either to fresh ovarian tissue homogenate or to acetonic powder of ovarian follicles induced a significant increase in the conversion of testosterone to oestradiol. Aromatase activity is four times greater in the presence of D-Asp than in its absence. However, almost equivalent values of the two K(m) values (both approximately 25 nmol l(-1)) indicate that aromatase has the same catalytic properties in both cases.

The distribution of nicotinamide adenine dinucleotide phosphate (NADPH)-d neurons and their relationship with nitric oxide synthase (NOS), vasoactive intestinal polypeptide (VIP), pituitary adenylate activating polypeptide (PACAP) and galanin (Gal) were examined in the gastrointestinal (GI) tract of the pigeon Columbia livia. NADPH-d-histochemistry, indirect immunofluorescence and confocal analysis were applied to cryosections. Western blot analysis was also applied on pigeon gut. NADPH-d neurons were found throughout the pigeon GI tract and they were evident in the myenteric, circular muscle and submucous plexuses. Positive varicose nerve fibres were also distributed within the longitudinal muscle layers and in the lamina propria of the mucosa. The stomach was the segment richest in positivities. The copresence VIP/Gal/NOS as well as PACAP/VIP were revealed in some NADPH-d-neurons. We suppose that the nitrergic nerve population of the pigeon GI tract belong to the muscle motility regulation as an inhibitory descending nerve pathway. Moreover the presence of VIP, Gal and PACAP in some NADPH-d-containing neurons enhances the inhibitory actions of these neurotransmitters whereas PACAP and Gal role is actually unknown.

It is well known that certain actions of androgen are mediated through in situ aromatization to estrogen in neural target tissues. This study was undertaken to investigate androgen utilization in the hypothalamus of the female frog, Rana esculenta, through a quantification of estrogen receptors and aromatase activity during the reproductive cycle. 3H-estradiol-binding molecules were present in both the cytosol and the nuclear extract of the hypothalamus. These molecules bound specifically 3H-estradiol with high affinity (Kd 10(-10) M) and low capacity (cytosol: 1.2+/-0.4 fmol/mg protein; nuclear extract: 7.9+/-0.6 fmol/mg protein). Aromatase activity was detected in the microsomal fraction of the hypothalamus using a sensitive in vitro radiometric assay. Both aromatase activity and nuclear estrogen receptor binding fluctuated in synchrony throughout the reproductive cycle. Western blot analysis of aromatase protein revealed one immunoreactive band with a molecular weight of approximately 56 kDa. In contrast to aromatase enzyme activity, the relative levels of aromatase protein changed little during the reproductive cycle suggesting that post-translational mechanisms may be involved in regulating estrogen synthesis in the frog brain. A possible role for estrogens in the modulation of the reproductive behavior in this species is suggested.

Testosterone receptors (AR) are present in the liver of the female green frog, Rana esculenta, which resolve into two fractions (A and B) by ion-exchange chromatography. Fraction A is primarily located in the nuclei, fraction B predominates in the cytosols, and both fractions show a high affinity and specificity for testosterone. Liver AR fraction levels vary dramatically during the frog sexual cycle. Fraction A levels are high only when the liver is engaged in vitellogenin production and the plasma testosterone levels are high: they are maximal when aromatase activity is most intense. Fraction B levels are high when the liver is not producing vitellogenin and the plasma testosterone levels are minimal. In addition, in vivo experiments carried out on ovariectomized females treated with testosterone show that testosterone induces both fraction A and liver aromatase activity. This induction may be a step in the process that allows the liver to obtain estrogen from plasma testosterone which induces vitellogenin synthesis.

Two forms of gonadotropin-releasing hormone (GnRH) were isolated from the gonads of the tunicate, Ciona intestinalis. The primary structure of the purified peptides was determined by MS and chemical sequence analysis. Both GnRH forms have blocked NH(2) and COOH termini, and their primary structures are identical to mammalian (mGnRH) and chicken I (cGnRH-I) forms reported previously in vertebrates. A total of 1.2 mg of purified cGnRH-I and 0.98 mg of mGnRH was obtained from 100 g of Ciona gonads. The physiological effects of native GnRHs included the induction of synthesis and secretion of sex steroids from ciona gonads and the secretion of luteinizing hormone from rat pituitary. These results suggest that the primary structure and functional roles of mGnRH and cGnRH-I have been highly conserved throughout evolution of chordates.

The distribution of galanin immunoreactive (Gal/IR) neurons was investigated in the gastrointestinal (GI) tract of the lizard Podarcis s. sicula. The indirect immunofluorescence method, image analysis and confocal analysis were applied to cryostat sections and whole mount preparations. Gal/IR nerve fibers and cell bodies were found throughout the lizard GI tract in the myenteric plexus, circular muscle layer and mucosa. These nerve structures decreased caudally. The stomach revealed a denser reactive nerve population than elsewhere. The projections of Gal/IR neurons were detected in the myenteric plexus of lizard gut using a confocal microscope which analyzed the immunoreactive material on the proximal and distal sides of muscle myotomies. An accumulation of Gal/IR material on the oral side of the myotomies demonstrated the oral-to-anal projection of Gal containing nerve structures. Based on our results, it can be hypothesized that Gal/IR neurons of the lizard digestive tract belong to the inhibitory descending pathway, which in most vertebrates is responsible for gut peristalsis regulation.

In this study, we show that sex hormones (testosterone, estradiol, and progesterone) act as physiological modulators of programmed cell death (PCD) during the frog liver involution observed postvitellogenesis. PCD in parenchymal cells is paralleled by the specific induction of the "tissue" transglutaminase (tTG) gene. tTG protein specifically accumulates in hepatocytes showing the morphological features of apoptosis. The hormone-dependent increase of both PCD and tTG was reproduced in ovariectomized frogs. Treatment of castrated animals with testosterone, estradiol, and progesterone inhibited the induction of both tTG and PCD, thus indicating that in vivo the drop in the circulating sex hormone is the signal favoring the involution phase of the maternal frog liver after mating. Although an affinity-purified polyclonal antibody raised against mammalian transglutaminase reacts in frog liver with a 55- to 60-kDa protein, concomitant with the onset of PCD, tTG cleavage products were detected, suggesting a proteolytic processing of the enzyme protein. These results represent the first evidence indicating that the physiological involution occurring postvitellogenesis of frog liver takes place by programmed cell death and that this, together with the concomitant induction of tTG gene expression, is regulated by sex hormones., (Copyright 1999 Academic Press.)

The distribution of neurons containing galanin immunoreactivity (Gal/IR) has been detected in the oviduct of the lizard Podarcis s. sicula during the main phases of its sexual cycle and after 17beta-estradiol treatment. Indirect immunofluorescence technique was applied both to cryostatic sections and whole mount preparations, and Western blot analysis, with an antibody directed against mammalian galanin (Gal), was performed with lizard oviduct extracts. Colocalization of Gal with vasoactive intestinal polypeptide (VIP) was also studied as well as Gal effects on egg deposition. In the quiescent oviduct of non-reproductive females, scanty Gal/IR fibres were found in the uterine-vaginal segment. During the reproductive period a gradual increase of positive nerve fibres and cell bodies were found distally in the lizard oviduct and the vagina revealed a reactive nerve population denser than elsewhere. Gal-IR nerve structures were present either in the musculature or mucosa and in the intermuscular layer they were organized in a nerve network. In the oviduct of non-reproductive females, 17beta-estradiol administration induced a significant increase of neurons containing Gal/IR. This hormone could be involved in the egg laying by means of galanin action and this hypothesis is supported by the induction of premature oviposition in pre-ovulatory females after Gal administration. Western blot analysis validates this peptide as true Gal, recognising one protein band with a molecular weight (3.2 kDa), similar to that of porcine Gal. Double labelling studies showed the co-presence of Gal and VIP in some neurons.

In this study for the first time we have characterized a progesterone receptor in the reproductive system of the female of Octopus vulgaris. Scatchard analysis revealed that one binding component with high affinity and low capacity for the ligand was present only in the nuclear extract. Competition experiments showed that the progesterone receptor was strictly specific for progesterone. DNA-cellulose binding and DEAE-Sephacel both confirmed the presence of one 3H-progesterone binding component which eluted at a salt concentration of 0.14 +/- 0.05 M NaCl and 0.15 +/- 0.05 M NaCl respectively. By using monoclonal antibodies against chicken progesterone receptor (subunits A and B), we have localized on Western Blot one band of about 70 kDa. Immunoreactivity for progesterone binding molecules has been localized in the nuclei of the follicle cells of the ovary, of the proximal portion of the oviduct and of the outer region of the nidimental gland. These data, taken together, provide evidence that in Octopus vulgaris the progesterone receptor has biochemical and immunohistochemical characteristics resembling those of progesterone receptor in vertebrates.

Lipoprotein fractions in Rana esculenta were separated using the same salt intervals currently applied for human lipoproteins. Very low density lipoproteins (VLDL), low density lipoproteins (LDL) and high density lipoproteins (HDL) were analyzed with reference to the electrophoretic pattern. The lipoprotein electrophoretic pattern in males and females throughout the reproductive cycle showed minor differences. In general, each fraction was characterized by a specific apolipoprotein content. VLDL and LDL fractions were dominated by a high molecular weight (MW) band, most likely the counterpart of human Apolipoprotein B (apo B). The apo B in R. esculenta cross reacted, although weakly, with antibodies raised against chicken apo B. The HDL fraction showed a band with an apparent MW of 29 kDa. The electrophoretic mobility of the protein moiety of HDL was similar to human apolipoprotein A-I (apo A-I). However, HDL apolipoprotein of R. esculenta did not cross react with antibodies against chicken apo A-I under either denaturing or native conditions. The HDL apolipoprotein of R. esculenta was purified by DEAE-Sephacel chromatography followed by HPLC. Its amino acid composition showed a moderate correlation with trout, salmon, chicken and human apo A-I.

In the green frog, Rana esculenta, a peculiar feature of female reproductive endocrinology is an high level of circulating testosterone. Although several hypotheses have been set out to explain this phenomenon, the testosterone specific roles in female anuran have not been yet fully explored. This study results propose a testosterone implication in liver vitellogenin synthesis control, since in ovariectomized frogs the hormone induces an increase of circulating vitellogenin. The testosterone action could depend on its local conversion to 17beta-estradiol by aromatase which is present in frog liver tissue. Liver aromatase activity ranges from 7.5 to 26 fmoles E2 formed/mg protein/h and results higher as long as liver is engaged in vitellogenin synthesis. Aromatase activity seems depend on testosterone since it decreases after ovariectomy and is restored by testosterone injection in ovariectomized frogs. In green frog liver, testosterone binding molecules are present both in cytosol and nuclei. These molecule binding properties (Kd and Bmax in nM range; t 1/2 = 85 min; specificity) are in line with those of testosterone receptor of other lower vertebrate target tissue. In liver nuclei, testosterone receptor level undergoes modification throughout the sexual cycle which almost coincides with that of plasma testosterone level and liver aromatase activity. This could indicate that the testosterone induction of liver aromatase in frogs is via the testosterone receptor, as reported for aromatase of mammalian brain tissues.

The effects of the proopiomelanocortin-derived opioid peptide, beta-endorphin (beta-EP), and of the opioid antagonist, naloxone (NAL), on both basal and pituitary-stimulated androgen secretion from superfused quiescent and active testes were assessed in the adult lizard, Podarcis sicula. In the absence of the homologous pituitary, in vitro treatment with beta-EP and/or NAL did not affect basal secretion of androgens from quiescent and active testes. Conversely, in the presence of the homologous pituitary, treatment with beta-EP brought about a decrease in androgen secretion in active testes, but no effect on quiescent ones. Naloxone counteracted the inhibitor effect of beta-EP in active testes, and enhanced maximal pituitary-stimulated secretion of androgens in quiescent but not in active testes. The effects produces by beta-endorphin and naloxone were reversible. These results suggest that, in this lizard, opioids might be involved in the control of androgen release. The lack of effect of beta-EP and naloxone when added directly to the testes seems to suggest that the opioid agonist and antagonist act on androgen release by modulating pituitary gonadotrophin output.

The present study reports the presence of progesterone, testosterone and 17 beta-estradiol and their corresponding binding proteins in the reproductive system of Octopus vulgaris Lam (phylum Mollusca, subphylum Cephalopoda). These sex hormones occur in testis, vas deferens, seminal vesicle, prostate and Needham's sac. The hemolymph also contains a small, but significant, amount of these hormones and their carrier proteins. Among various tissues of the reproductive system, the seminal vesicle possesses the highest concentration of progesterone (4.8 ng/g tissue). The testis is the organ which contains the highest amount of testosterone (5.2 ng/g) whereas the prostate is the organ which contains the highest amount of 17 beta-estradiol (0.92 ng/g). The presence of these hormones has been ascertained by a radioimmunoassay method, an immunoenzymatic method and by a chemical (HPLC) method. Seatchard studies indicated that vas deferens and seminal vesicle contain specific sex steroid binding molecules at affinity levels comparable to those of vertebrate steroid receptors (0.5-5.0 pmol/g protein). In addition to the presence of the hormones, the delta 5,3 beta hydroxysteroid dehydrogenase, the key enzyme of steroidogenesis, also is found in testis. From a phylogenetic point of view, these findings are very interesting because they indicate a common origin of a sex hormonal system between Mollusca Cephalopoda and Vertebrates.