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Start Over Author "Chen, Gen" Publication Type Academic Journals
1,223 results on '"Chen, Gen"'

4. Analysis of the genetic characteristics and variations in disease-resistant grass carp based on whole-genome resequencing and transcriptome sequencing

Author

Huifang Tan, Yude Wang, Biao Hu, Yongjie Zhang, An'min Liao, Wuxia Liu, Chen Gen, Kaikun Luo, Min Tao, Chun Zhang, Qinbo Qin, and Shaojun Liu

Subjects
Disease-resistant grass carp, Genetic characteristics, GISH, Whole-genome resequencing, RNA-Seq, Genetics, QH426-470, Reproduction, QH471-489, Animal biochemistry, QP501-801
Abstract

Disease-resistant grass carp (DR-GGC) was obtained by backcrossing gynogenetic grass carp (GGC, 2n = 48, female) with common grass carp (Ctenopharyngodon idellus [GC], 2n = 48, male). The genetic constitution and the potential mutation associated with growth dominance in DR-GGC were analyzed by genomic in situ hybridization (GISH), whole-genome resequencing (WGRS) and RNA-sequencing (RNA-seq). The GISH results showed partial translocations and deletions in the chromosomes of DR-GGC compared with GC. The WGRS results demonstrated that there were numerous genetic variations between DR-GGC and its parents, among which DR-GGC had the highest genomic heterozygosity and GGC had the highest genomic purity. The RNA-seq results revealed that the differentially expressed genes (DEGs) were significantly enriched in cellular processes and immune pathways in DR-GGC and GGC compared with GC. Meanwhile, the combined analysis of WGRS and RNA-seq identified DEGs with overlapping mutation loci in DR-GGC and GGC compared with GC. These DEGs were significantly enriched in the pathways of growth, metabolism, and immunity, including cellular metabolism, biosynthesis, glycolysis/gluconeogenesis, and extra cellular matrix (ECM) receptor interactions. These results provide a genetic basis for exploring the better growth characteristics and disease resistance of DR-GGC than GC, valuable information on the genetic breeding for grass carp, and a further understanding of the genetic characteristics of gynogenetic individuals.

Published
2024
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5. Teacher Feedback-Based Collaborative Testing Improves Students' Knowledge Gaps of Parasitology

Author

Peng, Xiaohong, Jiang, Liping, Cao, Deping, Chen, Gen, Li, Dayu, Teng, Pingying, and Li, Jianzhou

Abstract

Collaborative testing has been demonstrated the ability to improve students' performance, enhance students' learning, and aid in knowledge retention in many different courses. However, this examination mode lacks the process of teacher feedback. Herein, a short teacher feedback from was added immediately after the collaborative testing to improve the students' performance. A parasitology class of 121 undergraduates was randomized into two groups: "group A" and "group B." Collaborative testing was carried out at the end of theoretical teaching. During the test, students would first answer questions as individuals for 20 minutes. Then, students from "group A" answered the same questions in groups (5 students in each group) for 20 minutes, while the group-testing duration was only 15 minutes in "group B." Immediately after the group testing, teachers conducted a 5-minute feedback about the morphology identification according to the analysis of the answers by "group B." Four weeks later, a final test was conducted in an individual test. The total scores and scores for each examination content were analyzed. The results showed that there was no significant difference in the final exam scores between both groups (t = -1.278, P = 0.204). However, the morphological and diagnostic test results of the final examination in group B were significantly higher than those of the midterm examination, while there was no significant change in "group A" (t = 4.333, P = 0.051). The results confirmed that the teacher feedback after the collaborative testing can effectively make up for the students' knowledge gaps.

Published
2023
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36. Dual redox mediators accelerate the electrochemical kinetics of lithium-sulfur batteries.

Author

Liu, Fang, Sun, Geng, Wu, Hao Bin, Chen, Gen, Xu, Duo, Mo, Runwei, Shen, Li, Li, Xianyang, Ma, Shengxiang, Tao, Ran, Li, Xinru, Tan, Xinyi, Xu, Bin, Wang, Ge, Dunn, Bruce S, Sautet, Philippe, and Lu, Yunfeng

Abstract

The sluggish electrochemical kinetics of sulfur species has impeded the wide adoption of lithium-sulfur battery, which is one of the most promising candidates for next-generation energy storage system. Here, we present the electronic and geometric structures of all possible sulfur species and construct an electronic energy diagram to unveil their reaction pathways in batteries, as well as the molecular origin of their sluggish kinetics. By decoupling the contradictory requirements of accelerating charging and discharging processes, we select two pseudocapacitive oxides as electron-ion source and drain to enable the efficient transport of electron/Li+ to and from sulfur intermediates respectively. After incorporating dual oxides, the electrochemical kinetics of sulfur cathode is significantly accelerated. This strategy, which couples a fast-electrochemical reaction with a spontaneous chemical reaction to bypass a slow-electrochemical reaction pathway, offers a solution to accelerate an electrochemical reaction, providing new perspectives for the development of high-energy battery systems.

Published
2020

40. Statistical analysis of Bach’s Well-tempered Clavier

Author

Chen Gen-Fang

Subjects
Social Sciences
Abstract

Bach’s Well-tempered Clavier is the greatest work of Bach keyboard music, it contains 48 preludes and fugue, it is one of Bach’s representative works of music creation. This paper firstly inputs MIDI data of Bach’s Welltempered Clavier, and then constructs the analysis model of Bach’s Well-tempered Clavier. The experimental results show that the envelope of the number of occurrences of each musical note is in the shape of a double hump, indicating that the statistical number of musical notes played by the right hand or the left hand should be in the shape of a single camel peak in the musical range.

Published
2024
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