50 results on '"Chen, Lijiao"'
Search Results
2. The performance of VCS(volume, conductivity, light scatter) parameters in distinguishing latent tuberculosis and active tuberculosis by using machine learning algorithm
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Chen, Lijiao, Yuan, Lingke, Sun, Tingting, Liu, Ruiqing, Huang, Qing, and Deng, Shaoli
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- 2023
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3. Discovery and characterization of sgRNA-sequence-independent DNA cleavage from CRISPR/Cas9 in mouse embryos
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Yang, Liyun, Chen, Lijiao, Zheng, Yang, Deng, Li, Bai, Raoxian, Zhang, Ting, Wang, Zhengbo, and Li, Shangang
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- 2024
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4. EMC10 modulates hepatic ER stress and steatosis in an isoform-specific manner
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Chen, Kuangyang, Wang, Yahao, Yang, Jia, Klöting, Nora, Liu, Chuanfeng, Dai, Jiarong, Jin, Shuoshuo, Chen, Lijiao, Liu, Shan, Liu, Yuzhao, Yu, Yongzhuo, Liu, Xiaoxia, Miao, Qing, Liew, Chong Wee, Wang, Yangang, Dietrich, Arne, Blüher, Matthias, and Wang, Xuanchun
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- 2024
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5. Study on the bacteria consortium for treating tank cleaning oily sludge and its synergistic degradation mechanism
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Nie, Hongyun, Chen, Lijiao, Zhang, Qi, Guo, Dini, Yin, Qiuyue, Nie, Maiqian, Wang, Lei, and Guo, Yonghua
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- 2023
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6. The protective effects of Levilactobacillus brevis FZU0713 on lipid metabolism and intestinal microbiota in hyperlipidemic rats
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Fan, Xiaoyun, Zhang, Qing, Guo, Weiling, Wu, Qi, Hu, Jinpeng, Cheng, Wenjian, Lü, Xucong, Rao, Pingfan, Ni, Li, Chen, Youting, and Chen, Lijiao
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- 2023
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7. Integrated genome-wide chromatin accessibility and expression profile identify key transcription factors involved in bud endodormancy break in tea plants
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Chen, Lijiao, Wang, Miao, Wang, Baijuan, Chen, Siqin, Li, Limei, Liu, Zhiwei, Liu, Kunyi, Nian, Bo, Cai, Xiaobo, Jiang, Changjun, and Zhao, Ming
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- 2023
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8. Striking impacts of biomass burning on PM2.5 concentrations in Northeast China through the emission inventory improvement
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Chen, Lijiao, Gao, Yang, Ma, Mingchen, Wang, Lili, Wang, Qinglu, Guan, Shuhui, Yao, Xiaohong, and Gao, Huiwang
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- 2023
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9. The photocatalytic hydrogen evolution of g–C3N4/K0.5Na0.5NbO3 nanofibers heterojunction under visible light
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Zhang, Tianjin, Chen, Lijiao, Yuan, Changlai, Su, Mingwei, Liu, Xiao, Huang, Shuya, Jiang, Mingfang, Su, Kaiyuan, and Wang, Dezhi
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- 2023
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10. Multi-omics analysis of the metabolism of phenolic compounds in tea leaves by Aspergillus luchuensis during fermentation of pu-erh tea
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Ma, Yan, Jiang, Bin, Liu, Kunyi, Li, Ruoyu, Chen, Lijiao, Liu, Zhiwei, Xiang, Guisheng, An, Jiangshan, Luo, Hui, Wu, Jianlin, Lv, Caiyou, Pan, Yinghong, Ling, Tiejun, and Zhao, Ming
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- 2022
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11. Preparation of cationic modified graphene oxide and characterization of its adsorption for rhamnolipids from fermentation broth
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Nie, Hongyun, Chen, Lijiao, Chen, Jing, Nie, Maiqian, Diwu, Zhenjun, Wang, Lei, and Cui, Ying
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- 2022
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12. Recognition Model for Tea Grading and Counting Based on the Improved YOLOv8n.
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Xia, Yuxin, Wang, Zejun, Cao, Zhiyong, Chen, Yaping, Li, Limei, Chen, Lijiao, Zhang, Shihao, Wang, Chun, Li, Hongxu, and Wang, Baijuan
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TRANSFORMER models ,TEA ,TEA trade ,COUNTING ,RECOGNITION (Psychology) ,PROBLEM solving - Abstract
Grading tea leaves efficiently in a natural environment is a crucial technological foundation for the automation of tea-picking robots. In this study, to solve the problems of dense distribution, limited feature-extraction ability, and false detection in the field of tea grading recognition, an improved YOLOv8n model for tea grading and counting recognition was proposed. Firstly, the SPD-Conv module was embedded into the backbone of the network model to enhance the deep feature-extraction ability of the target. Secondly, the Super-Token Vision Transformer was integrated to reduce the model's attention to redundant information, thus improving its perception ability for tea. Subsequently, the loss function was improved to MPDIoU, which accelerated the convergence speed and optimized the performance. Finally, a classification-positioning counting function was added to achieve the purpose of classification counting. The experimental results showed that, compared to the original model, the precision, recall and average precision improved by 17.6%, 19.3%, and 18.7%, respectively. The average precision of single bud, one bud with one leaf, and one bud with two leaves were 88.5%, 89.5% and 89.1%. In this study, the improved model demonstrated strong robustness and proved suitable for tea grading and edge-picking equipment, laying a solid foundation for the mechanization of the tea industry. [ABSTRACT FROM AUTHOR]
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- 2024
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13. Modeling label-wise syntax for fine-grained sentiment analysis of reviews via memory-based neural model
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Zhao, Ling, Liu, Ying, Zhang, Mingyao, Guo, Tingting, and Chen, Lijiao
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- 2021
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14. Assisted Tea Leaf Picking: The Design and Simulation of a 6-DOF Stewart Parallel Lifting Platform.
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Wang, Zejun, Yang, Chunhua, Che, Raoqiong, Li, Hongxu, Chen, Yaping, Chen, Lijiao, Yuan, Wenxia, Yang, Fang, Tian, Juan, and Wang, Baijuan
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ADAPTIVE control systems ,TEA ,PARALLEL kinematic machines ,PLANT growth ,ROBOT motion - Abstract
The 6-DOF Stewart parallel elevation platform serves as the platform for mounting the tea-picking robotic arm, significantly impacting the operational scope, velocity, and harvesting precision of the robotic arm. Utilizing the Stewart setup, a parallel elevation platform with automated lifting and leveling capabilities was devised, ensuring precise halts at designated elevations for seamless harvesting operations. The effectiveness of the platform parameter configuration and the reasonableness of the posture changes were verified. Firstly, the planting mode and growth characteristics of Yunnan large-leaf tea trees were analyzed to determine the preset path, posture changes, and mechanism stroke of the Stewart parallel lifting platform, thereby determining the basic design specifications of the platform. Secondly, a 3D model was established using SolidWorks, a robust adaptive PD control model was built using MATLAB for simulation, and dynamic calculations were carried out through data interaction in Simulink and ADAMS. Finally, the rationality of the lifting platform design requirements was determined based on simulation data, a 6-DOF Stewart parallel lifting platform was manufactured, and a motion control system was built for experimental verification according to the design specifications and simulation data. The results showed that the maximum deviation angle around the X, Y, and Z axes was 10°, the maximum lifting distance was 15 cm, the maximum load capacity was 60 kg, the platform response error was within ±0.1 mm, and the stable motion characteristics reached below the millimeter level, which can meet the requirements of automated operation of the auxiliary picking robotic arm. [ABSTRACT FROM AUTHOR]
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- 2024
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15. Phospholipids composition and molecular species of large yellow croaker (Pseudosciaena crocea) roe
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Liang, Peng, Li, Ruifen, Sun, He, Zhang, Min, Cheng, Wenjian, Chen, Lijiao, Cheng, Xinwei, and Akoh, Casimir C.
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- 2018
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16. Characterization of mating-type idiomorphs suggests that Morchella importuna, Mel-20 and M. sextelata are heterothallic
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Chai, Hongmei, Chen, Lijiao, Chen, Weimin, Zhao, Qi, Zhang, Xiaolei, Su, Kaimei, and Zhao, Yongchang
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- 2017
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17. Effect of DHA-Enriched Phospholipids from Fish Roe on Rat Fecal Metabolites: Untargeted Metabolomic Analysis.
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Lu, Xiaodan, Huang, Luyao, Chen, Yanjun, Hu, Ling, Zhong, Rongbin, Chen, Lijiao, Cheng, Wenjian, Zheng, Baodong, and Liang, Peng
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FISH eggs ,METABOLOMICS ,LARIMICHTHYS ,LIPID metabolism disorders ,CALPROTECTIN ,METABOLITES ,ENTEROHEPATIC circulation ,BILE - Abstract
Lipid metabolism disorder has become an important hidden danger threatening human health, and various supplements to treat lipid metabolism disorder have been studied. Our previous studies have shown that DHA-enriched phospholipids from large yellow croaker (Larimichthys Crocea) roe (LYCRPLs) have lipid-regulating effects. To better explain the effect of LYCRPLs on lipid regulation in rats, the fecal metabolites of rats were analyzed from the level of metabolomics in this study, and GC/MS metabolomics measurements were performed to figure out the effect of LYCRPLs on fecal metabolites in rats. Compared with the control (K) group, 101 metabolites were identified in the model (M) group. There were 54, 47, and 57 metabolites in the low-dose (GA), medium-dose (GB), and high-dose (GC) groups that were significantly different from that of group M, respectively. Eighteen potential biomarkers closely related to lipid metabolism were screened after intervention with different doses of LYCRPLs on rats, which were classified into several metabolic pathways in rats, including pyrimidine metabolism, the citric acid cycle (TCA cycle), the metabolism of L-cysteine, carnitine synthesis, pantothenate and CoA biosynthesis, glycolysis, and bile secretion. L-cysteine was speculated to be a useful biomarker of LYCRPLs acting on rat fecal metabolites. Our findings indicated that LYCRPLs may regulate lipid metabolism disorders in SD rats by activating these metabolic pathways. [ABSTRACT FROM AUTHOR]
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- 2023
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18. MAFLD in Patients with Cushing's Disease Is Negatively Associated with Low Free Thyroxine Levels Rather than with Cortisol or TSH Levels.
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Chen, Kuangyang, Chen, Lijiao, Dai, Jiarong, and Ye, Hongying
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THYROTROPIN , *BIOMARKERS , *THYROID gland function tests , *CUSHING'S syndrome , *THYROXINE , *CROSS-sectional method , *AGE distribution , *NON-alcoholic fatty liver disease , *RISK assessment , *SEX distribution , *BODY mass index , *COMORBIDITY , *HYDROCORTISONE , *DISEASE risk factors ,ULTRASONIC imaging of the abdomen - Abstract
Purpose. This study aims to analyze the clinical characteristic of metabolic associated fatty liver disease (MAFLD) in patients with active Cushing's disease (CD) and determine associations of thyroid hormones with MAFLD. Methods. Patients with active CD were included in this cross-sectional study. All subjects were assessed for hepatic steatosis by abdominal ultrasonography and thyroid functions. Demographic and clinical characteristic parameters were collected for correlation analysis and logistic analysis. Results. 290 individuals with active CD were included in Huashan hospital from January 2014 to February 2022. We found that the prevalence of CD with MAFLD was 33.79%. The MAFLD group had a lower level of FT4 and a higher level of FT3/FT4 but no difference in levels of cortisol, 24 h UFC, TSH, TT4, TT3, and FT3. Correlation analysis showed positive associations of TSH, TT4, TT3, FT3, and FT3/FT4 with BMI. In age-, BMI-, sex-, cortisol-, and 24 h UFC-adjusted analysis, FT4 was independently associated with MAFLD in patients with CD. This association remained similar even after adjusting for the presence of metabolic syndrome components. Conclusion. Lower FT4 levels were associated with higher risk of MAFLD in patients with CD. FT4 may be used as a helpful indicator to predict MAFLD and provide novel ideas for the treatment of MAFLD in patients with CD in the future. [ABSTRACT FROM AUTHOR]
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- 2023
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19. An In Vitro Catalysis of Tea Polyphenols by Polyphenol Oxidase.
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Liu, Kunyi, Chen, Qiuyue, Luo, Hui, Li, Ruoyu, Chen, Lijiao, Jiang, Bin, Liang, Zhengwei, Wang, Teng, Ma, Yan, and Zhao, Ming
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POLYPHENOL oxidase ,POLYPHENOLS ,CATALYSIS ,GREEN tea ,TEA ,PHENOLIC acids - Abstract
Tea polyphenol (TPs) oxidation caused by polyphenol oxidase (PPO) in manufacturing is responsible for the sensory characteristics and health function of fermented tea, therefore, this subject is rich in scientific and commercial interests. In this work, an in vitro catalysis of TPs in liquid nitrogen grinding of sun-dried green tea leaves by PPO was developed, and the changes in metabolites were analyzed by metabolomics. A total of 441 metabolites were identified in the catalyzed tea powder and control check samples, which were classified into 11 classes, including flavonoids (125 metabolites), phenolic acids (67 metabolites), and lipids (55 metabolites). The relative levels of 28 metabolites after catalysis were decreased significantly (variable importance in projection (VIP) > 1.0, p < 0.05, and fold change (FC) < 0.5)), while the relative levels of 45 metabolites, including theaflavin, theaflavin-3′-gallate, theaflavin-3-gallate, and theaflavin 3,3′-digallate were increased significantly (VIP > 1.0, p < 0.05, and FC > 2). The increase in theaflavins was associated with the polymerization of catechins catalyzed by PPO. This work provided an in vitro method for the study of the catalysis of enzymes in tea leaves. [ABSTRACT FROM AUTHOR]
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- 2023
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20. Membrane-Bound EMC10 Is Required for Sperm Motility via Maintaining the Homeostasis of Cytoplasm Sodium in Sperm.
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Liu, Lijie, Mao, Shanhua, Chen, Kuangyang, Dai, Jiarong, Jin, Shuoshuo, Chen, Lijiao, Wang, Yahao, Guo, Lina, Yang, Yiting, Zhan, Chongwen, Xiong, Zuquan, Diao, Hua, Zhou, Yuchuan, Ding, Qiang, and Wang, Xuanchun
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SPERM motility ,HOMEOSTASIS ,SODIUM ,GERM cells ,MALE infertility ,CYTOPLASM ,SPERMATOZOA - Abstract
Endoplasmic reticulum membrane protein complex subunit 10 (EMC10) is an evolutionarily conserved and multifunctional factor across species. We previously reported that Emc10 knockout (KO) leads to mouse male infertility. Emc10-null spermatozoa exhibit multiple aspects of dysfunction, including reduced sperm motility. Two subunits of a Na/K-ATPase, ATP1A4 and ATP1B3, are nearly absent in Emc10 KO spermatozoa. Here, two isoforms of EMC10 were characterized in the mouse testis and epididymis: the membrane-bound (mEMC10) and secreted (scEMC10) isoforms. We present evidence that mEMC10, rather than scEMC10, is required for cytoplasm sodium homeostasis by positively regulating ATP1B3 expression in germ cells. Intra-testis mEMC10 overexpression rescued the sperm motility defect caused by Emc10 KO, while exogenous recombinant scEMC10 protein could not improve the motility of spermatozoa from either Emc10 KO mouse or asthenospermic subjects. Clinically, there is a positive association between ATP1B3 and EMC10 protein levels in human spermatozoa, whereas no correlation was proven between seminal plasma scEMC10 levels and sperm motility. These results highlight the important role of the membrane-bound EMC10 isoform in maintaining cytoplasm sodium homeostasis and sperm motility. Based on the present results, the mEMC10-Na, K/ATPase α4β3 axis is proposed as a novel mechanism underlying the regulation of cytoplasmic sodium and sperm motility, and its components seem to have therapeutic potential for asthenospermia. [ABSTRACT FROM AUTHOR]
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- 2022
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21. Corrosion behavior of chromium-free dacromet coating in seawater
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Hu, Huili, Li, Ning, Cheng, Jinning, and Chen, Lijiao
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- 2009
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22. Extensive humoral immune response to AAVs and Cas proteins in nonhuman primates.
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Xiao, Puhao, Bai, Raoxian, Zhang, Ting, Wu, Ruo, Chen, Lijiao, Hou, Yu, Shen, Bin, Niu, Yuyu, Li, Shangang, Ji, Weizhi, and Chen, Yongchang
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- 2021
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23. DHA-enriched phospholipids from large yellow croaker roe regulate lipid metabolic disorders and gut microbiota imbalance in SD rats with a high-fat diet.
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Lu, Xiaodan, Zhong, Rongbin, Hu, Ling, Huang, Luyao, Chen, Lijiao, Cheng, Wenjian, Zheng, Baodong, and Liang, Peng
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- 2021
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24. Effects of SCN1A and SCN2A polymorphisms on responsiveness to valproic acid monotherapy in epileptic children: A protocol for systematic review and meta-analysis.
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Zhuangfei Wen, Jiang Chen, Bin Zhu, Yan Lu, Lijiao Chen, Wen, Zhuangfei, Chen, Jiang, Zhu, Bin, Lu, Yan, and Chen, Lijiao
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- 2021
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25. Influence of Maillard Reaction in Volatile Flavor Compounds of Blue Round Scad (Decapterus maruadsi) Enzymatic Hydrolysate.
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Zhong, Rongbin, Lu, Xiaodan, Zhong, Ji, Chen, Lijiao, Cheng, Wenjian, and Liang, Peng
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MAILLARD reaction ,FLAVOR ,SULFUR compounds ,ODORS ,VOLATILE organic compounds ,PYRAZINES ,FURANS - Abstract
This study aimed to assess the effect of the Maillard reaction (MR) on volatile flavor compounds of blue round scad (Decapterus maruadsi) enzymatic hydrolysate (BRSEH). Enzymatic hydrolysates combined with reducing sugars (xylose and glucose) and cysteine were kept at 100°C for 30 min to produce Maillard reaction products (MRPs). The volatile flavor compounds of enzymatic hydrolysates and MRPs were determined by gas chromatography-mass spectrometry. The results showed that 97 volatile flavor compounds were found in blue round scad meat and BRSEHs. Aldehyde compounds were the most abundant class of compounds produced by the enzymatic hydrolysis, and 2,4-heptadienal should be responsible for the rich fishy odor of BRSEHs. Through MR, the volatile fragrance substance content of MRPs showed an increase when compared with BRSEHs. Fifty-nine volatile compounds were found in MRPs, e.g., furans, pyrazines, and sulphur-containing compounds, all of which contributed to the flavor improvement of BRSEHs. The results indicate that MR could be utilized to broaden the application of blue round scad and to modify the flavor characters of BRSEH. [ABSTRACT FROM AUTHOR]
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- 2021
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26. Quality assessment of large yellow croaker (Larimichthys crocea) roe oil before and after refining.
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Zhang, Lingyun, Wei, Wei, Huang, Luyao, Zheng, Tingting, Zhong, Rongbin, Pang, Jie, Chen, Lijiao, Cheng, Wenjian, and Liang, Peng
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- 2021
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27. Genetic variation and demographic history analysis of Pestalotiopsis, Pseudopestalotiopsis, and Neopestalotiopsis fungi associated with tea (Camellia sinensis) inferred from the internal transcribed spacer region of the nuclear ribosomal DNA.
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Chen, Lijiao, Li, Hongye, Jiao, Wengweng, Tao, Mei, Lv, Caiyou, Zhao, Ming, and Wang, Miao
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RIBOSOMAL DNA , *NUCLEAR DNA , *TEA , *MOLECULAR evolution , *DEXTROSE , *PESTALOTIOPSIS - Abstract
To elucidate the demographic history within and among the genera Pseudopestalotiopsis, Neopestalotiopsis, and Pestalotiopsis and their genetic diversity, we studied internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA (nrDNA) with no saturation effects in 172 Pestalotiopsis‐like fungal accessions isolated from Camellia sinensis planted in nine provinces in China (64 from Yunnan, 4 from Guangxi, 2 from Guizhou, 42 from Jiangxi, 3 from Sichuan, 3 from Zhejiang, 1 from Henan, 18 from Fujian, 12 from Chongqing, and 18 from other provinces) and in Japan (1), France (1), Turkey (1), and Thailand (2). Among these, 44 Pestalotiopsis‐like isolates from Yunnan in this study formed a clade in phylogenetic trees with Pestalotiopsis accessions in GenBank, despite their cultural characteristics on potato dextrose agar displaying no genus specificity. Based on estimated sequence polymorphism parameters of average pairwise differences (K), haplotype diversity (Hd), nucleotide diversity (Pi), θ (per site) from η, and recombination event rate (Rm), we identified moderate genetic variation in these three genera. Molecular evolution analysis with neutrality tests and mismatch distribution demonstrated that Pseudopestalotiopsis and Pestalotiopsis ribosomal ITS sequences evolved under a neutral model. However, the recent demographic expansion of the three genera promoted geographical isolation but showed no clear geographical structure. Pairwise genetic divergences between haplotypes and a median‐joining network revealed Yunnan or Jiangxi as a possible centre of origin, and that Pseudopestalotiopsis and Neopestalotiopsis may derive from Pestalotiopsis. [ABSTRACT FROM AUTHOR]
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- 2021
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28. The Effect of Simvastatin on Gut Microbiota and Lipid Metabolism in Hyperlipidemic Rats Induced by a High-Fat Diet.
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Zhang, Qing, Fan, Xiaoyun, Ye, Rui, Hu, Yuzhong, Zheng, Tingting, Shi, Rui, Cheng, Wenjian, Lv, Xucong, Chen, Lijiao, and Liang, Peng
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LIPID metabolism ,GUT microbiome ,AMINO acid metabolism ,HIGH-fat diet ,LIPID metabolism disorders ,SIMVASTATIN - Abstract
The objective of this study was to investigate the effects of simvastatin (SIM) on lipid metabolism disorders and gut microbiota in high-fat diet-induced hyperlipidemic rats. The obtained results revealed that feeding rats with SIM (20 mg/kg/day) significantly decreased serum lipid level and inhibited hepatic lipid accumulation and steatosis. Histological analysis further indicated that SIM reduced lipid deposition in adipocytes and hepatocytes in comparison with that of the HFD group. The underlying mechanisms of SIM administration against HFD-induced hyperlipidemia were also studied by UPLC-Q-TOF/MS-based liver metabonomics coupled with pathway analysis. Metabolic pathway enrichment analysis of liver metabolites with significant difference in abundance indicated that fatty acids metabolism and amino acid metabolism were the main metabolic pathways altered by SIM administration. Meanwhile, operational taxonomic units (OTUs) analysis revealed that oral administration of SIM altered the composition of gut microbiota, including Ruminococcaceae (OTU960) and Lactobacillus (OTU152), and so on. Furthermore, SIM treatment also regulated the mRNA levels of the genes involved in lipid and cholesterol metabolism. Immunohistochemistry (IHC) analysis of the liver-related proteins (CD36, CYP7A1 and SREBP-1C) showed that oral administration of SIM could regulate the levels of the protein expression related to hepatic lipid metabolism. [ABSTRACT FROM AUTHOR]
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- 2020
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29. A high performance liquid chromatography method for simultaneous detection of 20 bioactive components in tea extracts.
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Nian, Bo, Chen, Lijiao, Yi, Chao, Shi, Xingyun, Jiang, Bin, Jiao, Wenwen, Liu, Qianting, Lv, Caiyou, Ma, Yan, and Zhao, Ming
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- 2019
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30. Artificial intelligence-based platform for online teaching management systems.
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Zhao, Ling, Chen, Lijiao, Liu, Qing, Zhang, Mingyao, Copland, Henry, Yuan, Xiaohui, and Elhoseny, Mohamed
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LEARNING Management System , *DIFFERENTIAL evolution , *INTELLIGENT networks , *MATHEMATICAL models , *MACHINE learning , *ARTIFICIAL intelligence - Abstract
At present, artificial intelligence has developed rapidly and has been increasingly used in teaching, which has brought about earth-shaking changes in the education of various subjects. To further promote the intelligent development of college online courses, this paper studies the mathematical models and algorithms in the learning management system based on the knowledge forgetting curve. Through the analysis of the current situation of mathematical modeling and application of "knowledge forgetting curve," the method of fuzzy mathematical knowledge and differential modeling is used, the mathematical model and algorithm design of a new "knowledge forgetting curve" is proposed. And the English word review program test is designed. The results show that the use of the program can overcome the oblivion of the words. It can be seen that the "integrated intelligent teaching network platform" has great application prospects and potential benefits. [ABSTRACT FROM AUTHOR]
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- 2019
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31. English oral evaluation algorithm based on fuzzy measure and speech recognition.
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Zhao, Ling, Liu, Ying, Chen, Lijiao, Zhang, Junchi, Koomey, Jonathan G, Yuan, Xiaohui, and Elhoseny, Mohamed
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SPEECH perception ,FUZZY measure theory ,ORAL reading ,AUTOMATIC speech recognition ,LEARNING ability ,FUZZY algorithms ,EVALUATION methodology - Abstract
In oral English, for the pronunciation of continuous reading and easy to confuse, the difficulty to understand the pronunciation and so on, this kind of spoken pronunciation has a direct influence on the accuracy of computer speech recognition technology. Therefore, based on the fuzzy measure and speech recognition technology, in this paper, the English oral evaluation algorithm is studied. Based on fuzzy measure, the fuzzy comprehensive evaluation algorithm is discussed. This paper focuses on how to solve the problem that the fuzzy measure cannot be fused, the optimization and updating scheme proposed from the algorithm flow and evaluation model, and an effective oral reading evaluation model is established by effectively evaluating the different oral continuous state, giving the corresponding evaluation results. The simulation results show that the optimization of oral English evaluation algorithm can provide a scientific evaluation method and improvement strategy for oral teaching and improving oral learning ability. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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32. Determination of Fatty Acid Composition and Phospholipid Molecular Species of Large Yellow Croaker ( Pseudosciaena crocea ) Roe from China.
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Liang, Peng, Cheng, Xinwei, Xu, Yanping, Cheng, Wenjian, and Chen, Lijiao
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FATTY acids ,PHOSPHOLIPIDS ,PSEUDOSCIAENA - Abstract
The aim of this study is to investigate the composition of the fatty acids and phospholipids derived from large yellow croaker (Pseudosciaena crocea) roe. The composition of the total lipids and the molecular species of phospholipids were determined by gas chromatography–mass spectroscopy (GC–MS) and high performance liquid chromatography–evaporative light scattering detector (HPLC–ELSD), respectively. The results showed that large yellow croaker roe had high levels of the total lipid (19.6% ± 1.32%, w/w) and phospholipid (61.2% ± 1.22% of the total lipid). The phospholipid was rich in docosahexaenoic acid (31.0% ± 0.19% of the total phospholipids), and the major phospholipid molecular species was phosphatidylcholine (PC, 61.06% ± 0.02% of the total phospholipids, w/w). It was concluded that large yellow croaker roe is expected to be a good resource of phospholipids with a high content of PC. [ABSTRACT FROM PUBLISHER]
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- 2017
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33. Combined effects of proinflammatory cytokines and intermittent cyclic mechanical strain in inhibiting osteogenicity in human periodontal ligament cells.
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Sun, Chaofan, Chen, Lijiao, Shi, Xinlian, Cao, Zhensheng, Hu, Bibo, Yu, Wenbin, Ren, Manman, Hu, Rongdang, and Deng, Hui
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PERIODONTAL ligament , *CYTOKINES , *ALKALINE phosphatase , *TRANSCRIPTION factors , *OSTEOBLASTS , *ALIZARIN , *PERIODONTITIS - Abstract
Mechanical strain plays an important role in bone formation and resorption during orthodontic tooth movement. The mechanism has not been fully studied, and the process becomes complex with increased amounts of periodontal patients seeking orthodontic care. Our aims were to elucidate the combined effects of proinflammatory cytokines and intermittent cyclic strain (ICS) on the osteogenic capacity of human periodontal ligament cells. Cultured human periodontal ligament cells were exposed to proinflammatory cytokines (interleukin-1β 5 ng/mL and tumor necrosis factor-α 10 ng/mL) for 1 and 5 days, and ICS (0.5 Hz, 12% elongation) was applied for 4 h per day. The autocrine of inflammatory cytokines was measured by enzyme-linked immunosorbent assay. The expression of osteoblast markers runt-related transcription factor 2 and rabbit collagen type I was determined using real-time polymerase chain reaction and Western blot. The osteogenic capacity was also detected by alkaline phosphatase (ALP) staining, ALP activity, and alizarin red staining. We demonstrated that ICS impaired the osteogenic capacity of human periodontal ligament cells when incubated with proinflammatory cytokines, as evidenced by the low expression of ALP staining, low ALP activity, reduced alizarin red staining, and reduced osteoblast markers. These data, for the first time, suggest that ICS has a negative effect on the inductive inhibition of osteogenicity in human PDL cells mediated by proinflammatory cytokines. [ABSTRACT FROM AUTHOR]
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- 2016
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34. Analysis on correlation between coping styles and social support of first hospitalized patients with nasopharyngeal carcinoma.
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Peng Junhua, Lin Meihua, and Chen Lijiao
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- 2014
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35. Inhibition Effect of Triglyceride Accumulation by Large Yellow Croaker Roe DHA-PC in HepG2 Cells.
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Lu, Xiaodan, Zhong, Rongbin, Sun, He, Zheng, Baodong, Chen, Lijiao, Miao, Song, and Liang, Peng
- Abstract
The phospholipids (PLs) of large yellow croaker (Pseudosciaena crocea, P. crocea) roe contain a high level of polyunsaturated fatty acids, especially docosahexaenoic acid (DHA), which can lower blood lipid levels. In previous research, PLs of P. crocea roe were found able to regulate the accumulation of triglycerides. However, none of these involve the function of DHA-containing phosphatidylcholine (DHA-PC), which is the main component of PLs derived from P. crocea roe. The function by which DHA-PC from P. crocea roe exerts its effects has not yet been clarified. Herein, we used purified DHA-PC and oleic acid (OA) induced HepG2 cells to establish a high-fat model, and the cell activity and intracellular lipid levels were then measured. The mRNA and protein expression of Fatty Acid Synthase (FAS), Carnitine Palmitoyl Transferase 1A (CPT1A) and Peroxisome Proliferator-Activated Receptor α (PPARα) in HepG2 cells were detected via RT-qPCR and western blot as well. It was found that DHA-PC can significantly regulate triglyceride accumulation in HepG2 cells, the effect of which was related to the activation of PPARα receptor activity, upregulation of CPT1A, and downregulation of FAS expression. These results can improve the understanding of the biofunction of hyperlipidemia mediated by DHA-PC from P. crocea roe, as well as provide a theoretical basis for the utilization of DHA-PC from P. crocea roe as a functional food additive. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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36. Effect of inoculation with Penicillium chrysogenum on chemical components and fungal communities in fermentation of Pu-erh tea.
- Author
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Liu, Kunyi, Wang, Liyan, Jiang, Bin, An, Jiangshan, Nian, Bo, Wang, Daoping, Chen, Lijiao, Ma, Yan, Wang, Xinghua, Fan, Jiakun, Luo, Hui, Pan, Yinghong, and Zhao, Ming
- Subjects
- *
FOOD aroma , *FOOD fermentation , *FUNGAL communities , *PENICILLIUM chrysogenum , *GAS chromatography/Mass spectrometry (GC-MS) , *HIGH performance liquid chromatography , *FERMENTATION , *MYCOBACTERIUM tuberculosis - Abstract
[Display omitted] • P. chrysogenum used in pure-culture and enhanced fermentation of Pu-erh tea. • 69 compounds in enhanced fermentation were significantly lower. • 40 compounds in enhanced fermentation were significantly higher. • Inoculation with P. chrysogenum changed the composition of the fungal community. • Inoculation with P. chrysogenum improved the sensory quality of Pu-erh tea. Developing an effective method to improve the quality of Pu-erh tea is of great scientific and commercial interest. In this work, Penicillium chrysogenum P1 isolated from Pu-erh tea was inoculated in sterilized or unsterilized sun-dreid green tea leaves to develop pure-culture fermentation (PF) and enhanced fermentation (EF) of Pu-erh tea. Spectrophotometry and high performance liquid chromatography determined that contents of free amino acids (FAA), total tea polyphenols and eight polyphenolic compounds in PF were significantly lower than these in non-inoculation control test (CK) (P < 0.05), whereas the contents of soluble sugars and theabrownins (TB) in PF were significantly higher (P < 0.05) than in CK. A total of 416 volatile compounds were detected by headspace solid-phase micro-extraction combined with gas chromatography-mass spectrometry. Comparison to CK, 124 compounds in PF were degraded or decreased significantly [Variable importance in projection [(VIP) > 1.0, P < 0.05, fold change (FC) < 0.5], whereas 110 compounds in PF were formed or increased significantly (VIP > 1.0, P < 0.05, FC > 2). Compared with normal fermentation (NF), the levels of gallic acid, (+)-catechin, (−)-epicatechin and 64 volatile compounds in EF were significantly lower (VIP > 1.0, P < 0.05, FC < 0.5), whereas the levels of FAA and 39 volatile compounds were significantly higher (VIP > 1.0, P < 0.05, FC > 2). Amplicon sequencing of fungal internal transcribed spacer 1 (ITS1) revealed that P. chrysogenum P1 didn't become the dominant fungus in EF; while the fungal communities in EF were different from those in NF, in that the relative abundances of Blastobotrys bambusae and P. chrysogenum in EF were higher, and the relative abundances of Aspergillus niger and Kluyveromyces marxianus in EF were lower. Overall, inoculation of P. chrysogenum in unsterilized sun-dreid green tea leaves changed the the fungal communities in fermentation of Pu-erh tea, and chemical compounds in fermented tea leaves, i.e., the levels of TB and the compounds responsible for the stale flavor, e.g., 2-amino-4-methoxybenzothiazole were increased, resulting in improvement of the sensory quality, including mellower taste and stronger stale flavor. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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37. Serum scEMC10 Levels are Negatively Associated With Resting Metabolic Rate and age in Humans.
- Author
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Chen K, Dai J, Klöting N, Cao X, Jin S, Chen L, Wang Y, Liu S, Hu Y, Jiang L, Liew CW, Blüher M, and Wang X
- Subjects
- Humans, Animals, Mice, Middle Aged, Adult, Cross-Sectional Studies, Obesity metabolism, Overweight metabolism, Body Mass Index, Energy Metabolism, Calorimetry, Indirect, Basal Metabolism, Body Composition
- Abstract
Context: We have recently shown that the secreted isoform of endoplasmic reticulum membrane complex subunit 10 (scEMC10) is upregulated in human obesity and that overexpression of scEMC10 promotes, whereas antibody neutralization of circulating scEMC10 prevents diet-induced obesity in mice., Objective: To explore associations of serum scEMC10 with body mass index (BMI), resting metabolism rate (RMR), and age in humans., Design: A cross-sectional study., Setting and Patients: A total of 833 participants from a Chinese physical examination cohort and 191 participants from the Leipzig Obesity Biobank cohort., Main Outcome Measures: Serum scEMC10 concentrations are measured using chemiluminescent immunoassay. RMR is calculated based on measurements from indirect calorimetry with an open-circuit ventilated-hood system., Results: In the Chinese physical examination cohort, a J-shaped nonlinear correlation between BMI and serum scEMC10 was identified in participants where underweight, overweight, and obese people all presented higher serum scEMC10 levels than normal weight people. Participants younger than age 30 years old exhibited significantly higher serum scEMC10 levels than those older than 50 years of age. In addition, participants aged 30 to 40 years also had significantly higher serum scEMC10 levels than those aged 50 to 60 years. In the Leipzig Obesity Biobank cohort, we observed a significantly negative correlation between serum scEMC10 and resting energy expenditure after adjusting for BMI. Participants in the highest quartile of serum scEMC10 levels had significantly lower RMR than those in the first quartile. RMR had an independently inverse association with serum scEMC10., Conclusions: Serum scEMC10 levels are negatively associated with age and RMR in humans., (© The Author(s) 2023. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
- Published
- 2023
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38. [Development of a tau-V337M mouse model using CRISPR/Cas9 system and enhanced ssODN-mediated recombination].
- Author
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Chen L, Deng L, Sun W, Liu J, Zhang T, and Li S
- Subjects
- Animals, Male, Female, Mice, Humans, Rad51 Recombinase genetics, Mice, Inbred C57BL, Disease Models, Animal, Recombination, Genetic, CRISPR-Cas Systems genetics, RNA, Guide, CRISPR-Cas Systems
- Abstract
The generation of a tau-V337M point mutation mouse model using gene editing technology can provide an animal model with fast disease progression and more severe symptoms, which facilitate the study of pathogenesis and treatment of Alzheimer's disease (AD). In this study, single guide RNAs (sgRNA) and single-stranded oligonucleotides (ssODN) were designed and synthesized in vitro . The mixture of sgRNA, Cas9 protein and ssODN was microinjected into the zygotes of C57BL/6J mice. After DNA cutting and recombination, the site homologous to human 337 valine (GTG) in exon 11 was mutated into methionine (ATG). In order to improve the efficiency of recombination, a Rad51 protein was added. The female mice mated with the nonvasectomy male mice were used as the surrogates. Subsequently, the 2-cell stage gene edited embryos were transferred into the unilateral oviduct, and the F0 tau-V337M mutation mice were obtained. Higher mutation efficiency could be obtained by adding Rad51 protein. The F0 tau-V337M point mutation mice can pass the mutation on to the F1 generation mice. In conclusion, this study successfully established the first tau-V337M mutation mouse by using Cas9, ssODN and Rad51. These results provide a new method for developing AD mice model which can be used in further research on the pathogenesis and treatment of AD.
- Published
- 2023
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39. Efficient Modulation of Exon Skipping via Antisense Circular RNAs.
- Author
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Ren S, Huang M, Bai R, Chen L, Yang J, Zhang J, Guo W, Ji W, and Chen Y
- Abstract
Splice-switching antisense oligonucleotides (ASOs) and engineered U7 small nuclear ribonucleoprotein (U7 Sm OPT) are the most commonly used methods for exon skipping. However, challenges remain, such as limited organ delivery and repeated dosing for ASOs and unknown risks of by-products produced by U7 Sm OPT. Here, we showed that antisense circular RNAs (AS-circRNAs) can effectively mediate exon skipping in both minigene and endogenous transcripts. We also showed a relatively higher exon skipping efficiency at the tested Dmd minigene than U7 Sm OPT. AS-circRNA specifically targets the precursor mRNA splicing without off-target effects. Moreover, AS-circRNAs with adeno-associated virus (AAV) delivery corrected the open reading frame and restored the dystrophin expression in a mouse model of Duchenne muscular dystrophy. In conclusion, we develop an alternative method for regulating RNA splicing, which might be served as a novel tool for genetic disease treatment., (Copyright © 2023 Shuaiwei Ren et al.)
- Published
- 2023
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40. Evidence-Based Nursing Model in Interventional Thrombolysis for Acute Lower Extremity Arterial Embolism.
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Yang M, Chen L, Zhang M, Huang X, Zhao W, and Wang H
- Subjects
- Evidence-Based Nursing, Humans, Lower Extremity blood supply, Retrospective Studies, Thrombolytic Therapy adverse effects, Thrombolytic Therapy methods, Treatment Outcome, Embolism drug therapy, Embolism etiology, Peripheral Arterial Disease drug therapy, Peripheral Arterial Disease etiology
- Abstract
Acute lower extremity arterial embolism (AE) is a serious clinical emergency, and, if not treated in time, it can easily lead to limb ischemia and necrosis and eventually facing amputation, which seriously damages patients' physical and mental health. In the past, the conventional drug thrombolytic therapy had slow and limited efficacy, and the best time for treatment is easily delayed, while arterial dissection and thrombectomy treatment, although fast, is traumatic and has many complications, which is not easily accepted by patients. The aim of this study was to investigate the value of evidence-based care model in the application of interventional thrombolysis for acute lower limb arterial embolism. Seventy-two patients with acute lower limb arterial embolism who underwent interventional thrombolysis treatment received by the Department of Vascular Surgery of our hospital from July 2016 to December 2021 were randomly divided into a control group (given conventional nursing services) and a quality group (given full quality nursing services) to compare the effect of nursing services in the two groups. The results showed that the postoperative psychological status of patients in the quality group was significantly better than that of patients in the control group ( P < 0.05). The total incidence of postoperative adverse events and the total treatment efficiency of the quality group were better than those of the control group ( P < 0.05). The efficacy of quality nursing care in patients with acute lower extremity arterial embolism is more desirable than conventional nursing care and is recommended. The site of vascular occlusion after bypass surgery can be clarified when angiography is performed after thrombolytic therapy, which can help secondary surgical intervention to prolong the time to patency. The efficacy of quality nursing care in patients with acute lower extremity arterial embolism is more desirable than that of conventional nursing care and is recommended., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2022 Meijuan Yang et al.)
- Published
- 2022
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41. Predictive Value of the Peripheral Blood Parameters for Preeclampsia.
- Author
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Liao D, Chen L, Li Q, Liu G, Wang W, Li J, and Deng S
- Subjects
- Blood Platelets, Female, Humans, Leukocyte Count, Lymphocytes, Mean Platelet Volume, Neutrophils, Platelet Count, Pregnancy, ROC Curve, Retrospective Studies, Pre-Eclampsia diagnosis
- Abstract
Background: Excessive systemic inflammation plays a vital role in pathophysiology of preeclampsia (PE). The aim is to clarify the predictive value of the peripheral blood parameters including white blood cell (WBC), neutrophils, lymphocyte, monocyte, platelet count, mean platelet volume (MPV), plateletcrit, platelet distribution width (PDW), platelet-large cell ratio (PLCR), and the ratio value for PE., Methods: This retrospective study enrolled 170 PE patients, 123 healthy control pregnant women, and 122 non-pregnant women. When pregnant women were admitted to the hospital for delivery, peripheral complete blood cell count was detected by an automatic blood cell analyzer. Clinical signs and demographic characteristics were recorded. The receiver operating characteristic (ROC) curve was used to determine the cutoff value and analyze the predictive significances for PE. Furthermore, the risk factors of PE were tested by univariate and stratified analyses., Results: This study showed that WBC, neutrophil count, neutrophil percentage, NLR, NMR, and PLR# were significantly increased in PE patients as compared with pregnant control patients (p < 0.001), whereas lymphocyte percentage, monocyte percentage, and PNR were decreased. In addition, there was no significant difference in the rest of the peripheral blood parameters between women with and without PE. The ROC curve result revealed that WBC and neutrophil count had a higher AUC value than the rest of peripheral blood variables. WBC and neutrophil count are positively correlated MAP. Moreover, the WBC and neutrophil count were indicated as independent risk factors for the development of PE., Conclusions: This study clarifies that peripheral blood parameters of WBC and neutrophil count have good applied value with high sensitivity and specificity in predicting the development of PE and are also independent risk factors for the development of PE.
- Published
- 2022
- Full Text
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42. Systematic review and meta-analysis of the safety and effectiveness of low molecular heparin for severe acute pancreatitis.
- Author
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Wang H, Xu Y, Yang M, Zheng D, and Chen L
- Subjects
- Acute Disease, Humans, Length of Stay, Treatment Outcome, Heparin adverse effects, Pancreatitis drug therapy
- Abstract
Background: Because the effect of low molecular heparin (LMH) on acute ulcer and bleeding complications in patients with severe acute pancreatitis (SAP) is unclear, we investigated the safety and efficacy of early intervention with LMH in patients with SAP., Methods: Using the keywords "heparin", "low molecular weight heparin", "pancreatitis", and "severe acute pancreatitis", we searched PubMed, Medline, CNKI, etc. And select the reference documents of the comparative study of traditional treatment and low molecular weight heparin intervention. RevMan was used for the meta-analysis., Results: A total of 8 references were included in the study, and most of them were low risk bias (medium and high quality). Meta-analysis shows that, The MHS between the two groups is statistically heterogeneous. (Chi2=19.59, I2=95%, P<0.00001), Fixed-effects model (FEM) analysis showed that the MHS of experimental subjects was obviously shorter than that of controls (Z=3.24, P=0.001); The acute physiology and chronic health score (APACHE II) of the two groups were heterogeneous (Chi2=7.24, I2=72%, P=0.03); No heterogeneity was found in the amount of bleeding (Chi2=5.83, I2=31%, P=0.21), FEM analysis showed the number of complications in the experimental group was significantly less than that in the control group (Z=2.70, P=0.007)., Discussion: LMH intervention can dramatically reduce the average hospital stay and complications of patients with SAP, improve treatment efficacy, and has high safety.
- Published
- 2021
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43. The VCS Parameters of Lymphocytes may Improve Discrimination between Bacterial Infection and Viral Infection.
- Author
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Chen L, Liao D, Jiang J, Sun T, and Deng S
- Subjects
- Humans, Lymphocytes, Neutrophils, Retrospective Studies, Sensitivity and Specificity, Bacterial Infections diagnosis, Virus Diseases diagnosis
- Abstract
Background: Distinguishing bacterial infections from viral infections is very important for accurate and appro-priate drug treatment, alleviating diseases and avoiding side effects caused by drug abuse. The aim of this study is to assess the clinical usefulness of the lymphocyte VCS (volume, conductivity, light scatter) parameters to dis-tinguish bacterial infection from viral infection., Methods: Peripheral blood was collected from 60 viral infection patients (VIG), 63 bacterial infection patients (BIG), and 95 healthy controls (HC). The lymphocyte VCS parameters and blood routine indicators were obtained by using a hematology analyzer with VCS technology. The critical cutoff value, sensitivity and specificity were established based on receiver operator characteristic (ROC) curve analysis., Results: Mean volume of lymphocytes (MV-LY), median angle light scatter of lymphocytes (MALS-LY), upper median angle light scatter of lymphocytes (UMALS-LY), neutrophil-lymphocyte ratio (NLR) were significantly increased in the bacterial infection group compared with the viral infection group and the healthy controls. The area under curve (AUC) for mean volume of lymphocytes (MV-LY) was 0.8143 for discriminating the bacterial infection group from the viral infection group. For median angle light scatter of lymphocytes (MALS-LY), the area under curve (AUC) was 0.8116. For upper median angle light scatter of lymphocytes (UMALS-LY), the area under curve (AUC) was 0.8631. For neutrophil-lymphocyte ratio (NLR), the area under curve (AUC) was 0.8513., Conclusions: This study clarifies that mean volume of lymphocytes, median angle light scatter of lymphocytes, and upper median angle light scatter of lymphocytes have good clinical practical value in distinguishing bacterial infection from viral infection and healthy controls because of its high sensitivity and specificity.
- Published
- 2021
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44. Effects of SCN1A and SCN2A polymorphisms on responsiveness to valproic acid monotherapy in epileptic children: A protocol for systematic review and meta-analysis.
- Author
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Wen Z, Chen J, Zhu B, Lu Y, and Chen L
- Subjects
- Anticonvulsants therapeutic use, Child, Drug Resistance genetics, Electroencephalography, Epilepsy diagnosis, Epilepsy genetics, Humans, Meta-Analysis as Topic, Polymorphism, Single Nucleotide, Systematic Reviews as Topic, Treatment Outcome, Valproic Acid therapeutic use, Anticonvulsants pharmacology, Epilepsy drug therapy, NAV1.1 Voltage-Gated Sodium Channel genetics, NAV1.2 Voltage-Gated Sodium Channel genetics, Valproic Acid pharmacology
- Abstract
Background: : The gene mutation of coding sodium channel is one of the most important mechanisms in the pathogenesis of epilepsy. There exists a large inter-individual variation in the efficacy of valproic acid (VPA) against epilepsy. What are the genetic polymorphism influences of sodium channels on VPA response is still under discussion. In this study, a meta-analysis was used to further explore the effects of SCN1A and SCN2A gene polymorphism on VPA response in children with epilepsy., Methods: : The PubMed, EMBASE, Web of Science, Chinese National Knowledge Infrastructure, Chinese Science and Technique Journals Database, China Biology Medicine disc, and Wan Fang Database were searched up to April 2021 for appropriate studies regarding the association between SCN1A and SCN2A gene polymorphism on VPA response in children suffering from epilepsy. The meta-analysis was conducted by Review Manager 5.3 software., Results: : The results of this meta-analysis will be submitted to a peer-reviewed journal for publication., Conclusion: : This meta-analysis will summarize the effects of SCN1A and SCN2A gene polymorphisms on VPA response in children with epilepsy., Osf Registration Number: DOI 10.17605/OSF.IO/N2786., Competing Interests: The authors have no conflicts of interest to disclose., (Copyright © 2021 the Author(s). Published by Wolters Kluwer Health, Inc.)
- Published
- 2021
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45. Alternative Splicing Regulation of Anthocyanin Biosynthesis in Camellia sinensis var. assamica Unveiled by PacBio Iso-Seq.
- Author
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Chen L, Shi X, Nian B, Duan S, Jiang B, Wang X, Lv C, Zhang G, Ma Y, and Zhao M
- Subjects
- Alternative Splicing, Anthocyanins metabolism, Gene Expression Regulation, Plant, Plant Leaves metabolism, Plant Proteins genetics, Plant Proteins metabolism, Protein Isoforms, Camellia sinensis genetics
- Abstract
Although the pathway and transcription factor regulation of anthocyanin biosynthesis in tea plants [ Camellia sinensis (L.) O. Ktze] are known, post-transcriptional regulation mechanisms involved in anthocyanin accumulation have not been comprehensively studied. We obtained the full-length transcriptome of a purple cultivar ('Zijuan') and a normal green cultivar ('Yunkang 10#) of C. sinensis var. asssamica (Masters) showing different accumulation of anthocyanins and catechins through PacBio isoform sequencing (Iso-Seq). In total, 577,557 mapped full-length cDNAs were obtained, and 2,600 average-length gene isoforms were identified in both cultivars. After gene annotations and pathway predictions, we found that 98 key genes in anthocyanin biosynthesis pathways could have undergone alternative splicing (AS) events, and identified a total of 238 isoforms involved in anthocyanin biosynthesis. We verified expression of the C4H , CHS , FLS , CCOM , F3'5'H , LAR , PAL , CCR , CYP73A13 , UDP75L12 , UDP78A15/UFGT , UDP94P1 , GL3 , MYB113 , ANR , ANS , F3H , 4CL1 , CYP98A3/C3H , CHI , DFR genes and their AS transcripts using qRT-PCR. Correlation analysis of anthocyanin biosynthesis and gene expression results revealed that C4H1 , FLS1 , PAL2 , CCR2 , UDP75L122 and MYB113-1 are crucial AS transcripts for regulating anthocyanin biosynthesis in C. sinensis var. assamica Our results reveal post-transcriptional regulation of anthocyanin biosynthesis in tea plants, and provide more new insights into the regulation of secondary metabolism., (Copyright © 2020 Chen et al.)
- Published
- 2020
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46. High Performance Liquid Chromatography and Metabolomics Analysis of Tannase Metabolism of Gallic Acid and Gallates in Tea Leaves.
- Author
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Liu M, Xie H, Ma Y, Li H, Li C, Chen L, Jiang B, Nian B, Guo T, Zhang Z, Jiao W, Liu Q, Ling T, and Zhao M
- Subjects
- Aspergillus niger chemistry, Aspergillus niger enzymology, Camellia sinensis chemistry, Camellia sinensis metabolism, Carboxylic Ester Hydrolases chemistry, Chromatography, High Pressure Liquid methods, Fermentation, Fungal Proteins chemistry, Gallic Acid chemistry, Metabolomics methods, Plant Leaves chemistry, Plant Leaves metabolism, Plant Leaves microbiology, Aspergillus niger metabolism, Camellia sinensis microbiology, Carboxylic Ester Hydrolases metabolism, Fungal Proteins metabolism, Gallic Acid metabolism
- Abstract
Tannase (E.C. 3.1.1.20) is hypothesized to be involved in the metabolism of gallates and gallic acid (GA) in pu-erh tea fermentation. In this work, we measured tannase in Aspergillus niger fermented tea leaves and confirmed the production of fungal tannase during pu-erh tea fermentation. A decrease in catechin and theaflavin gallates and a significant increase in GA content and the relative peak areas of ethyl gallate, procyanidin A2, procyanidin B2, procyanidin B3, catechin-catechin-catechin, epiafzelechin, and epicatechin-epiafzelechin [variable importance in the projection (VIP) > 1.0, p < 0.05, and fold change (FC) > 1.5] were observed using high performance liquid chromatography (HPLC) and metabolomics analysis of tea leaves fermented or hydrolyzed by tannase. In vitro assays showed that hydrolysis by tannase or polymerization of catechins increased the antioxidant activity of tea leaves. In summary, we identified a metabolic pathway for gallates and their derivatives in tea leaves hydrolyzed by tannase as well as associated changes in gallate and GA concentrations caused by fungal tannase during pu-erh tea fermentation.
- Published
- 2020
- Full Text
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47. Tensile strength suppresses the osteogenesis of periodontal ligament cells in inflammatory microenvironments.
- Author
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Sun C, Liu F, Cen S, Chen L, Wang Y, Sun H, Deng H, and Hu R
- Subjects
- Adolescent, Adult, Alkaline Phosphatase metabolism, Calcification, Physiologic, Cells, Cultured, Cytokines metabolism, Extracellular Matrix metabolism, Female, Humans, Immunohistochemistry, Inflammation Mediators metabolism, Male, Periodontitis etiology, Periodontitis metabolism, Periodontitis pathology, Phenotype, Young Adult, Cellular Microenvironment, Osteogenesis, Periodontal Ligament cytology, Stem Cells cytology, Stem Cells metabolism, Tensile Strength
- Abstract
The present study aimed to investigate the role of orthodontic force in osteogenesis differentiation, matrix deposition and mineralization in periodontal ligament cells (PDLCs) cells in inflammatory microenvironments. The mesenchymal origin of PDLCs was confirmed by vimentin and cytokeratin staining. PDLCs were exposed to inflammatory cytokines (5 ng/ml IL‑1β and 10 ng/ml TNF‑α) and/or tensile strength (0.5 Hz, 12% elongation) for 12, 24 or 48 h. Cell proliferation and tensile strength‑induced cytokine expression were assessed by MTT assay and ELISA, respectively. Runt‑related transcription factor 2 (RUNX2) and type I collagen (COL‑I) expression were analysed by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Additionally, alkaline phosphatase activity was measured, and the mineralization profile was evaluated by alizarin red S staining. PDLCs exposed to tensile strength in inflammatory microenvironments exhibited reduced proliferation and mineralization potential. Treatment with the inflammatory cytokines IL‑1β and TNF‑α increased RUNX2 expression levels; however, decreased COL‑I expression levels, indicating that bone formation and matrix deposition involve different mechanisms in PDL tissues. Notably, RUNX2 and COL‑I expression levels were decreased in PDLCs exposed to a combination of an inflammatory environment and loading strength. The decreased osteogenic potential in an inflammatory microenvironment under tensile strength suggests that orthodontic force may amplify periodontal destruction in orthodontic patients with periodontitis.
- Published
- 2017
- Full Text
- View/download PDF
48. Proteomic Analysis of the Effect of DHA-Phospholipids from Large Yellow Croaker Roe on Hyperlipidemic Mice.
- Author
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Liang P, Zhang M, Cheng W, Lin W, and Chen L
- Subjects
- Animals, Docosahexaenoic Acids metabolism, Electrophoresis, Gel, Two-Dimensional, Humans, Hyperlipidemias metabolism, Liver chemistry, Liver metabolism, Male, Mass Spectrometry, Mice, Mice, Inbred ICR, Perciformes metabolism, Phospholipids metabolism, Proteins chemistry, Proteins metabolism, Proteomics, Docosahexaenoic Acids administration & dosage, Eggs analysis, Hyperlipidemias drug therapy, Phospholipids administration & dosage
- Abstract
Previously, we found that phospholipids derived from large yellow croaker (Pseudosciaena crocea) roe had a higher level of docosahexaenoic acid (DHA-PL), which had beneficial effects on lipid metabolism. However, the mechanism by which DHA-PL from P. crocea roe exerts these effects has not yet been illuminated. Herein, we investigated the underlying molecular action of DHA-PL by examining changes in liver protein expression in control, hyperlipidemic, and DHA-PL-treated mice. A total of 16 proteins, 9 up-regulated and 7 down-regulated, were identified and classified into several metabolic pathways, such as fat digestion and absorption, peroxisome proliferator activated receptor (PPAR) signaling, and antigen processing and presentation; the largest functional class found was that of fat digestion and absorption. We revealed Apoa1 to be a biomarker of DHA-PL effects on hyperlipidemic mice by DHA-PL diet. These results not only improve our current understanding of hyperlipidemic regulation by DHA-PL, but also suggest that DHA-PL should be applied as a beneficial food additive.
- Published
- 2017
- Full Text
- View/download PDF
49. The 5-HT 2A receptor potassium-chloride cotransporter 2 signaling pathway in a rat incision pain model.
- Author
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Dong R, Yu B, Chen L, and Yu W
- Abstract
Postoperative pain is a critical problem in clinical pain administration. Due to the unclear formation mechanism of postoperative pain, the treatment of postoperative pain is still in the symptomatic treatment stage and lacking satisfactory analgesic effect. Postoperative pain can be simulated by using a rat incision pain model. We observed changes in pain-related behavior of rats affected by the 5-hydroxytryptamine 2A receptor (5-HT
2A R) agonist, TCB-2, and antagonist, ketanserin, through intrathecal delivery. The transcription and translation level of potassium-chloride cotransporter 2 (KCC2) in the spinal cord was also measured to investigate the role of the 5-HT2A R-KCC2 pathway in the mechanism of incision pain. Compared with the control group, rats in the incision pain group had decreased mechanical withdrawal threshold (MWT), with significant differences on day 1-7 after surgery, and significant decreases in thermal withdrawal latency (TWL) on days 1, 2, 3 and 6 (P<0.05). Compared with the incision + dimethyl sulfoxide (DMSO) group, MWT and TWL decreased in the incision + ketanserin group on day 1 and 2 (P<0.05). There was no significant difference detected in TWL of incision + TCB-2 group on day 1, while MWT increased significantly compared to the incision + DMSO group (P<0.05). Furthermore, the transcription and translation levels of KCC2 in the incision + ketanserin group decreased significantly in comparison to the incision + DMSO group (P<0.05), while an increase was detected in the incision + TCB-2 group (P<0.05). MWT and TWL decreased in the incision pain rats, accompanied with a decreased transcription and translation level of KCC2. Intrathecal delivery of the 5-HT2A R agonist, TCB-2, alleviated the decreased WMT and inhibited the decreased transcription and translation level of KCC2, while intrathecal delivery of the 5-HT2A R antagonist, ketanserin, aggravated the decreased WMT and transcription and translation levels of KCC2, suggesting the involement of the 5-HT2A R-KCC2 pathway in the formation mechanism of incision pain in rats.- Published
- 2016
- Full Text
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50. Improving endoglucanase activity by adding the carbohydrate-binding module from Corticium rolfsii.
- Author
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Tang Z, Chen H, Chen L, Liu S, Han X, and Wu Q
- Subjects
- Basidiomycota genetics, Cellulase chemistry, Cellulase genetics, Cellulose metabolism, Enzyme Stability, Escherichia coli genetics, Escherichia coli metabolism, Gene Expression, Hydrogen-Ion Concentration, Hydrolysis, Receptors, Cell Surface chemistry, Receptors, Cell Surface genetics, Recombinant Fusion Proteins chemistry, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Temperature, Basidiomycota enzymology, Cellulase metabolism, Receptors, Cell Surface metabolism
- Abstract
The carbohydrate-binding module (CBM) is an important domain of most cellulases that plays a key role in the hydrolysis of cellulose. The neutral endoglucanase (EG1) gene was reconstructed. A redesigned endoglucanase, named EG2, was constructed with a CBM containing a linker from Corticium rolfsii (GenBank Accession No. D49448). The redesigned EG genes were expressed in Escherichia coli, and their characteristics are discussed. Results showed that the degradation of cellulose by EG2 was about double that by EG1. The specific activities of EG1 and EG2 were tested under optimal conditions, and EG2 had higher activity (169.1 ± 2.74 U/mg) toward CMC-Na than did EG1 (84.0 ± 1.98) in the process of cellulose degradation. The optimal pH and temperature, pH stability, and heat stability of EG1 and EG2 were similar. Results indicated that the CBM plays an essential role in the hydrolysis of cellulose. We can improve EG's catalytic power by adding the CBM from Corticium rolfsii.
- Published
- 2014
- Full Text
- View/download PDF
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