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Your search keyword '"Chen, Xiao-Long"' showing total 373 results
Start Over Author "Chen, Xiao-Long" Publication Type Academic Journals
373 results on '"Chen, Xiao-Long"'

17. YAP/TAZ Signaling Enhances Angiogenesis of Retinal Microvascular Endothelial Cells in a High-Glucose Environment.

Author

Wang, Xing-li, Xian, Yang, and Chen, Xiao-long

Abstract

Diabetic retinopathy (DR) is a major cause of irreversible blindness in the working-age population. Neovascularization is an important hallmark of advanced DR. There is evidence that Yes-associated protein (YAP)/transcriptional co-activator with a PDZ binding domain (TAZ) plays an important role in angiogenesis and that its activity is regulated by vascular endothelial growth factor (VEGF). Therefore, the aim of this study was to investigate the effect of YAP/TAZ-VEGF crosstalk on the angiogenic capacity of human retinal microvascular endothelial cells (hRECs) in a high-glucose environment. The expression of YAP and TAZ of hRECs under normal conditions, hypertonic conditions and high glucose were observed. YAP overexpression (OE-YAP), YAP silencing (sh-YAP), VEGF overexpression (OE-VEGF) and VEGF silencing (sh-VEGF) plasmids were constructed. Cell counting kit-8 assay was performed to detect cells proliferation ability, transwell assay to detect cells migration ability, and tube formation assay to detect tube formation ability. The protein expression of YAP, TAZ, VEGF, matrix metalloproteinase (MMP)-8, MMP-13, vessel endothelium (VE)-cadherin and alpha smooth muscle actin (α-SMA) was measured by western blot. The proliferation of hRECs was significantly higher in the high glucose group compared with the normal group, as well as the protein expression of YAP and TAZ (p < 0.01). YAP and VEGF promoted the proliferation, migration and tube formation of hRECs in the high glucose environment (p < 0.01), and increased the expression of TAZ, VEGF, MMP-8, MMP-13 and α-SMA while reducing the expression of VE-cadherin (p < 0.01). Knockdown of YAP effectively reversed the above promoting effects of OE-VEGF (p < 0.01) and overexpression of YAP significantly reversed the inhibition effects of sh-VEGF on above cell function (p < 0.01). In a high-glucose environment, YAP/TAZ can significantly promote the proliferation, migration and tube formation ability of hRECs, and the mechanism may be related to the regulation of VEGF expression. [ABSTRACT FROM AUTHOR]

Published
2024
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32. Is Preoperative Fibrinogen Associated with the Survival Prognosis of Gastric Cancer Patients? A Multi-centered, Propensity Score-Matched Retrospective Study

Author

Zhao, Lin-Yong, Zhao, Yong-Liang, Wang, Jun-Jiang, Zhao, Qi-Di, Yi, Wen-Qi, Yuan, Qing, Chen, Xin-Zu, Li, Yong, Yang, Kun, Chen, Xiao-Long, Zhang, Wei-Han, Liu, Kai, Pang, Hua-Yang, Galiullin, Danil, Wang, Hui, Sun, Li-Fei, Song, Xiao-Hai, Zheng, Jia-Bin, Yao, Xue-Qing, Zhou, Zong-Guang, Yu, Pei-Wu, and Hu, Jian-Kun

Published
2020
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