116 results on '"Choi, Young‐Bong"'
Search Results
2. Autophagy-competent mitochondrial translation elongation factor TUFM inhibits caspase-8-mediated apoptosis
- Author
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Choi, Chang-Yong, Vo, Mai Tram, Nicholas, John, and Choi, Young Bong
- Published
- 2022
- Full Text
- View/download PDF
3. Phosphorylation of the selective autophagy receptor TAX1BP1 by TBK1 and IKBKE/IKKi promotes ATG8-family protein-dependent clearance of MAVS aggregates.
- Author
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White, Jesse, Choi, Young Bong, Zhang, Jiawen, Vo, Mai Tram, He, Chaoxia, Shaikh, Kashif, and Harhaj, Edward W.
- Subjects
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RNA virus infections , *VESICULAR stomatitis , *VIRUS diseases , *LYSOSOMES , *AUTOPHAGY - Abstract
TAX1BP1 is a selective macroautophagy/autophagy receptor that inhibits NFKB and RIGI-like receptor (RLR) signaling to prevent excessive inflammation and maintain homeostasis. Selective autophagy receptors such as SQSTM1/p62 and OPTN are phosphorylated by the kinase TBK1 to stimulate their selective autophagy function. However, it is unknown if TAX1BP1 is regulated by TBK1 or other kinases under basal conditions or during RNA virus infection. Here, we found that TBK1 and IKBKE/IKKi function redundantly to phosphorylate TAX1BP1 and regulate its autophagic turnover through canonical macroautophagy. TAX1BP1 phosphorylation promotes its localization to lysosomes, resulting in its degradation. Additionally, we found that during vesicular stomatitis virus infection, TAX1BP1 is targeted to lysosomes in an ATG8-family protein-independent manner. Furthermore, TAX1BP1 plays a critical role in the clearance of MAVS aggregates, and phosphorylation of TAX1BP1 controls its MAVS aggrephagy function. Together, our data support a model whereby TBK1 and IKBKE license TAX1BP1-selective autophagy function to inhibit MAVS and RLR signaling.
Abbreviations: ATG: autophagy related; BafA1: bafilomycin A1; CALCOCO2: calcium binding and coiled-coil domain 2; GFP: green fluorescent protein; IFA: indirect immunofluorescence assay; IFN: interferon; IκB: inhibitor of nuclear factor kappa B; IKK: IκB kinase; IRF: interferon regulatory factor; KO: knockout; LAMP1: lysosomal associated membrane protein 1; LIR: LC3-interacting region; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MAVS: mitochondrial antiviral signaling protein; MEF: mouse embryonic fibroblast; MOI: multiplicity of infection; IKBKG/NEMO: inhibitor of nuclear factor kappa B kinase regulatory subunit gamma; NFKB: nuclear factor kappa B; OPTN: optineurin; Poly(I:C): polyinosinic-polycytidylic acid; RB1CC1/FIP200: RB1 inducible coiled-coil 1; RIGI: RNA sensor RIG-I; RLR: RIGI-like receptor; SDD-AGE: semi-denaturing detergent-agarose gel electrophoresis; SeV: Sendai virus; SLR: SQSTM1-like receptor; SQSTM1: sequestosome 1; TAX1BP1: Tax1 binding protein 1; TBK1: TANK binding kinase 1; TNF: tumor necrosis factor; TRAF: TNF receptor associated factor; VSV: vesicular stomatitis virus; ZnF: zinc finger. [ABSTRACT FROM AUTHOR]- Published
- 2024
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4. A Hybrid Biofuel Cell with High Power and Operational Stability Using Electron Transfer‐Intensified Mediators and Multi‐Interaction Assembly.
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Jang, Younjun, Seo, Tae‐Won, Pak, Junha, Park, Moon Kyu, Ahn, Jeongyeon, Jin, Gee Chan, Lee, Seung Woo, Chung, Yoon Jang, Choi, Young‐Bong, Kwon, Cheong Hoon, and Cho, Jinhan
- Subjects
CHARGE exchange ,GLUCOSE oxidase ,METAL nanoparticles ,BIOMASS energy ,CATHODES - Abstract
Biofuel cells (BFCs) offer an eco‐friendly route to convert biochemical energy into electricity. However, their performance is hindered by insufficient enzyme immobilization as well as limited electron transfer within the enzymatic electrode. While the incorporation of redox mediators (RMs) into enzyme layers has been shown to improve BFC performance through enhanced electron transfer, progress has plateaued in the last decade. Herein, a major breakthrough is presented realized by a novel strategy that exploits electron transfer‐intensified RM layers. Metal nanoparticles covalently bridged between neighboring RMs facilitate electron transfer ubiquitously. Electron transfer characteristics are enhanced not only within the RM layers themselves, but also at the glucose oxidase (GOx)/host electrode and GOx/GOx interfaces. This leads to a remarkable performance boost in the enzymatic anode. A hybrid BFC constructed with innovative anode and Pt‐based cathode exhibits a striking combination of high power output (2.3 and 8.5 mW cm−2 at 10 and 300 mmol L−1 glucose, respectively) and exceptional operational stability (≈80% and 47% power retention after 10 days and 1 month, respectively), outperforming all previously reported BFCs by a significant margin. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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5. Diagnostic evaluation of qRT-PCR-based kit and dPCR-based kit for COVID-19
- Author
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Lee, Cherl-Joon, Shin, Wonseok, Mun, Seyoung, Yu, Minjae, Choi, Young-Bong, Kim, Dong Hee, and Han, Kyudong
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- 2021
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6. Efficacy and safety of topical Streptococcus postbiotic emollient in adolescents and adults with mild‐to‐moderate atopic dermatitis: A randomized, double‐blind, vehicle‐controlled trial.
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Kim, Min Seo, Kim, Hyeon Jin, Kang, So Min, Heo, Young Mok, Kang, Jiseung, Ryu, Tae Kyeong, Kim, Hyun Jeong, Choi, Young‐Bong, Kim, Sol, Nho, Youn Hwa, Kang, Seunghyun, Smith, Lee, Koyanagi, Ai, Papadopoulos, Nikolaos G., Jo, Hyungwoo, Lee, Dong‐Geol, Shin, Jung U, and Yon, Dong Keon
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ATOPIC dermatitis ,ADULTS ,ECZEMA ,STREPTOCOCCUS ,TEENAGERS - Abstract
A study was conducted to evaluate the effectiveness and safety of a topical emollient in treating mild-to-moderate atopic dermatitis (AD) in adolescents and adults. The study involved 100 participants and lasted for 24 weeks. The results showed that the group using the emollient had significant improvements in various measures compared to the placebo group. No safety concerns were reported. However, the study had limitations and further research is needed to explore other aspects of AD and the effects of the emollient. Overall, the emollient shows promise as a therapeutic option for long-term care of AD patients. [Extracted from the article]
- Published
- 2024
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7. Ultrasonic synthesis and characterization of poly(acrylamide)-co-poly(vinylimidazole)@MWCNTs composite for use as an electrochemical material
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Jeon, Won-Yong, Choi, Young-Bong, and Kim, Hyug-Han
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- 2018
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8. Author Correction: Performance of a glucose-reactive enzyme-based biofuel cell system for biomedical applications
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Jeon, Won-Yong, Lee, Jung-Hwan, Dashnyam, Khandmaa, Choi, Young-Bong, Kim, Tae-Hyun, Lee, Hae-Hyoung, Kim, Hae-Won, and Kim, Hyug-Han
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- 2019
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9. A Mediator‐Free Multi‐Ply Biofuel Cell Using an Interfacial Assembly between Hydrophilic Enzymes and Hydrophobic Conductive Oxide Nanoparticles with Pointed Apexes.
- Author
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Kang, Minchul, Nam, Donghyeon, Ahn, Jeongyeon, Chung, Yoon Jang, Lee, Seung Woo, Choi, Young‐Bong, Kwon, Cheong Hoon, and Cho, Jinhan
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- 2023
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10. Performance of a glucose-reactive enzyme-based biofuel cell system for biomedical applications
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Jeon, Won-Yong, Lee, Jung-Hwan, Dashnyam, Khandmaa, Choi, Young-Bong, Kim, Tae-Hyun, Lee, Hae-Hyoung, Kim, Hae-Won, and Kim, Hyug-Han
- Published
- 2019
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11. Activation of NIX-mediated mitophagy by an interferon regulatory factor homologue of human herpesvirus
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Vo, Mai Tram, Smith, Barbara J., Nicholas, John, and Choi, Young Bong
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- 2019
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12. Enhanced Modification between Glucose Dehydrogenase and Mediator Using Epoxy Silane Assembly for Monitoring Glucose.
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Seo, Tae-Won, Jeon, Won-Yong, and Choi, Young-Bong
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BLOOD sugar monitors ,BLOOD sugar monitoring ,GLUCOSE oxidase ,INDIUM tin oxide ,GLUCOSE ,HYPERGLYCEMIA ,EPOXY resins ,PEOPLE with diabetes - Abstract
Blood glucose monitoring (BGM) using disposable electrodes is commonly used in healthcare diagnosis. The BGM method is not suitable for people with diabetes requiring real-time monitoring who might experience sudden hypoglycemia or hyperglycemia owing to a single measurement at a specific moment. This study aimed to achieve an enhanced stability of glucose diagnosis for continuous glucose measurement systems (CGMs). A representative mediator of a second-generation glucose sensor was synthesized and coordinated with a polymer for immobilization on an indium tin oxide (ITO) electrode. For electrode immobilization, an electrode for enhanced stability was fabricated using the silanization method. The morphological properties of the electrodes were confirmed via cyclic voltammetry (CV), impedance spectroscopy, and SEM. The loss rate of the current density was only 10.11% of the initial current after 8 d. The electrode exhibited a coefficient of determination of R
2 = 0.9924, sensitivity of 1.5454 μA/cm2 ·mM, limit of quantitation (LOQ) of 7.604 μM, and limit of detection (LOD) of 2.509 μM for glucose concentrations between 0.1 and 20.0 mM. The electrode system developed in this study is applicable to the CGM healthcare industry and is expected to be applicable to biofuel cells. [ABSTRACT FROM AUTHOR]- Published
- 2023
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13. Stability of carbon nanotube yarn biofuel cell in human body fluid
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Kwon, Cheong Hoon, Lee, Jae Ah, Choi, Young-Bong, Kim, Hyug-Han, Spinks, Geoffrey M., Lima, Márcio D., Baughman, Ray H., and Kim, Seon Jeong
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- 2015
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14. The mitophagy receptor NIX induces vIRF-1 oligomerization and interaction with GABARAPL1 for the promotion of HHV-8 reactivation-induced mitophagy.
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Vo, Mai Tram, Choi, Chang-Yong, and Choi, Young Bong
- Subjects
INTERFERON receptors ,TYPE I interferons ,KAPOSI'S sarcoma-associated herpesvirus ,INTERFERON regulatory factors ,OLIGOMERIZATION ,VIRAL proteins - Abstract
Recently, viruses have been shown to regulate selective autophagy for productive infections. For instance, human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus (KSHV), activates selective autophagy of mitochondria, termed mitophagy, thereby inhibiting antiviral innate immune responses during lytic infection in host cells. We previously demonstrated that HHV-8 viral interferon regulatory factor 1 (vIRF-1) plays a crucial role in lytic replication-activated mitophagy by interacting with cellular mitophagic proteins, including NIX and TUFM. However, the precise molecular mechanisms by which these interactions lead to mitophagy activation remain to be determined. Here, we show that vIRF-1 binds directly to mammalian autophagy-related gene 8 (ATG8) proteins, preferentially GABARAPL1 in infected cells, in an LC3-interacting region (LIR)-independent manner. Accordingly, we identified key residues in vIRF-1 and GABARAPL1 required for mutual interaction and demonstrated that the interaction is essential for mitophagy activation and HHV-8 productive replication. Interestingly, the mitophagy receptor NIX promotes vIRF-1-GABARAPL1 interaction, and NIX/vIRF-1-induced mitophagy is significantly inhibited in GABARAPL1-deficient cells. Moreover, a vIRF-1 variant defective in GABARAPL1 binding substantially loses the ability to induce vIRF-1/NIX-induced mitophagy. These results suggest that NIX supports vIRF-1 activity as a mitophagy mediator. In addition, we found that NIX promotes vIRF-1 aggregation and stabilizes aggregated vIRF-1. Together, these findings indicate that vIRF-1 plays a role as a viral mitophagy mediator that can be activated by a cellular mitophagy receptor. Author summary: In addition to their role in energy metabolism, mitochondria act as platforms for antiviral signaling that leads to apoptosis and type I interferon expression in response to virus infection. However, for their benefit, viruses have evolved strategies to attenuate mitochondria-mediated antiviral signaling, including selective autophagy of mitochondria (termed mitophagy) that eliminates dysfunctional or superfluous mitochondria. We previously demonstrated that mitochondria-localized viral interferon regulatory factor 1 (vIRF-1) plays a role in the activation of mitophagy during human herpesvirus 8 (HHV-8) via interaction with the mitophagy proteins NIX and TUFM, thereby inhibiting antiviral responses and contributing to productive replication. However, it remains to elucidate the precise molecular mechanisms and regulation of vIRF-1-mediated mitophagy, particularly interactions with the core autophagy machinery. In this report, we discover that vIRF-1 interacts selectively with GABARAPL1 among ATG8 proteins in a non-canonical manner in lytically HHV-8-infected cells, and the mitophagy receptor NIX promotes vIRF-1 oligomerization and interaction with the ATG8 protein. Our results reveal the presence of a functional complex of vIRF-1/NIX/GABARAPL1 for the promotion of mitophagy and also provide the first evidence of the post-translational regulation of viral mitophagic protein by cellular mitophagy receptor. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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15. Genome-Wide Pathway Exploration of the Epidermidibacterium keratini EPI-7 T.
- Author
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Oh, Yunseok, Mun, Seyoung, Choi, Young-Bong, Jo, HyungWoo, Lee, Dong-Geol, and Han, Kyudong
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OROTIC acid ,GENOMICS ,NUCLEOTIDE sequencing ,BOTANY ,SEQUENCE analysis ,VITAMIN B2 - Abstract
Functional cosmetics industries using skin microbiome screening and beneficial materials isolated from key microorganisms are receiving increasing attention. Since Epidermidibacterium keratini EPI-7
T was first discovered in human skin, previous studies have confirmed that it can produce a new pyrimidine compound, 1,1′-biuracil, having anti-aging effects on human skin. Therefore, we conducted genomic analyses to judge the use value of E. keratini EPI-7T and provide up-to-date information. Whole-genome sequencing analysis of E. keratini EPI-7T was performed to generate new complete genome and annotation information. E. keratini EPI-7T genome was subjected to comparative genomic analysis with a group of closely-related strains and skin flora strains through bioinformatic analysis. Furthermore, based on annotation information, we explored metabolic pathways for valuable substances that can be used in functional cosmetics. In this study, the whole-genome sequencing (WGS) and annotation results of E. keratini EPI-7T were improved, and through comparative analysis, it was confirmed that the E. keratini EPI-7T has more metabolite-related genes than comparison strains. In addition, we annotated the vital genes for biosynthesis of 20 amino acids, orotic acid, riboflavin (B2) and chorismate. In particular, we were able to prospect that orotic acid could accumulate inside E. keratini EPI-7T under uracil-enriched conditions. Therefore, through a genomics approach, this study aims to provide genetic information for the hidden potential of E. keratini EPI-7T and the strain development and biotechnology utilization to be conducted in further studies. [ABSTRACT FROM AUTHOR]- Published
- 2023
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16. Functional implications of mitochondrial reactive oxygen species generated by oncogenic viruses
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Choi, Young Bong and Harhaj, Edward William
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- 2014
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17. Multifaceted roles of TAX1BP1 in autophagy.
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White, Jesse, Suklabaidya, Sujit, Vo, Mai Tram, Choi, Young Bong, and Harhaj, Edward W.
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ZINC-finger proteins ,MYELOID differentiation factor 88 ,HTLV ,HTLV-I ,TUMOR necrosis factor receptors ,GABA receptors ,AUTOPHAGY - Abstract
TAX1BP1 is a selective macroautophagy/autophagy receptor that plays a central role in host defense to pathogens and in regulating the innate immune system. TAX1BP1 facilitates the xenophagic clearance of pathogenic bacteria such as Salmonella typhimurium and Mycobacterium tuberculosis and regulates TLR3 (toll-like receptor 3)-TLR4 and DDX58/RIG-I-like receptor (RLR) signaling by targeting TICAM1 and MAVS for autophagic degradation respectively. In addition to these canonical autophagy receptor functions, TAX1BP1 can also exert multiple accessory functions that influence the biogenesis and maturation of autophagosomes. In this review, we will discuss and integrate recent findings related to the autophagy function of TAX1BP1 and highlight outstanding questions regarding its functions in autophagy and regulation of innate immunity and host defense. Abbreviations: ATG: autophagy related; CALCOCO: calcium binding and coiled-coil domain; CC: coiled-coil; CHUK/IKKα: conserved helix-loop-helix ubiquitous kinase; CLIR: noncanonical LC3-interacting region; GABARAP: gamma-aminobutyric acid receptor associated protein; HTLV-1: human T-lymphotropic virus 1; IFN: interferon; IL1B/IL1β: interleukin 1 beta; LIR: LC3-interacting region; LPS: lipopolysaccharide; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; MAPK/JNK: mitogen-activated protein kinase; mATG8: mammalian Atg8 homolog; MAVS: mitochondrial antiviral signaling protein; MEF: mouse embryonic fibroblast; MTB: Mycobacterium tuberculosis; MYD88: myeloid differentiation primary response gene 88; NBR1: NBR1, autophagy cargo receptor; NFKB/NF-κB: nuclear factor of kappa light polypeptide gene enhancer in B cells; OPTN: optineurin; Poly(I:C): polyinosinic:polycytidylic acid; PTM: post-translational modification; RB1CC1: RB1-inducible coiled-coil 1; RIPK: receptor (TNFRSF)-interacting serine-threonine kinase; RLR: DDX58/RIG-I-like receptor; RSV: respiratory syncytia virus; SKICH: SKIP carboxyl homology; SLR: SQSTM1 like receptor; SQSTM1: sequestosome 1; TAX1BP1: Tax1 (human T cell leukemia virus type I) binding protein 1; TBK1: TANK-binding kinase 1; TICAM1: toll-like receptor adaptor molecule 1; TLR: toll-like receptor; TNF: tumor necrosis factor; TNFAIP3: TNF alpha induced protein 3; TNFR: tumor necrosis factor receptor; TOM1: target of myb1 trafficking protein; TRAF: TNF receptor-associated factor; TRIM32: tripartite motif-containing 32; UBD: ubiquitin binding domain; ZF: zinc finger. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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18. Voltammetric detection of trimethylamine using immobilized trimethylamine dehydrogenase on an electrodeposited goldnanoparticle electrode
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Choi, Young-Bong, Kim, Hee Gon, Han, Gui Hwan, Kim, Hyug-Han, and Kim, Si Wouk
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- 2011
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19. A Real-Time Detection Device for the Rapid Quantification of Skin Casual Sebum Using the Oil Red O Staining Method.
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Ahn, Kung, Han, Sangjin, Yun, Kyeongeui, Lee, Wooseok, Lee, Dong-Geol, Kang, So Min, Choi, Young-Bong, Han, Kyudong, and Ahn, Yong Ju
- Subjects
STAINS & staining (Microscopy) ,SEBUM ,FILAGGRIN ,HUMAN skin color ,PLASTIC films - Abstract
The human skin sebum suggests that it (along with other epidermal surface lipids) plays a role in skin barrier formation, the moderation of cutaneous inflammation, and antimicrobial defense. Various methods have been developed for collecting and measuring skin sebum. We tested methods of detection using "color intensity", by staining the skin casual sebum. This process was conducted in three steps; first, the selection of materials for sebum collection; second, staining the collected sebum; third, the development of a device that can measure the level of stained sebum. A plastic film was used to effectively collect sebum that increased with the replacement time of the sebum. In addition, the collected sebum was stained with Oil Red O (ORO) and checked with RGB; as a result, the R
2 value was higher than 0.9. It was also confirmed that the correlation value was higher than 0.9 in the comparison result with Sebumeter® , which is a common standard technology. Finally, it was confirmed that the R2 value was higher than 0.9 in the detection value using the sensor. In conclusion, we have proven the proof of concept (PoC) for this method, and we would like to introduce an effective sebum measurement method that differs from the existing method. [ABSTRACT FROM AUTHOR]- Published
- 2022
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20. The high throughput screening of neuropeptide FF2 receptor ligands from Korean herbal plant extracts
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Do, Ernest U., Piao, Long Zhu, Choi, Gyu, Choi, Young Bong, Kang, Tong Mook, Shin, Jaekyoon, Chang, Yung-Jin, Nam, Hee-Young, Kim, Ho-Jin, and Kim, Su-il
- Published
- 2006
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21. The E3/E4 ubiquitin conjugation factor UBE4B interacts with and ubiquitinates the HTLV-1 Tax oncoprotein to promote NF-κB activation.
- Author
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Mohanty, Suchitra, Han, Teng, Choi, Young Bong, Lavorgna, Alfonso, Zhang, Jiawen, and Harhaj, Edward William
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UBIQUITINATION ,HTLV-I ,SYNTAXINS ,ADULT T-cell leukemia ,UBIQUITIN ,CELL communication - Abstract
Human T-cell leukemia virus type 1 (HTLV-1) is the etiological agent of adult T-cell leukemia/lymphoma (ATLL), and the neurological disease HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The HTLV-1 Tax protein persistently activates the NF-κB pathway to enhance the proliferation and survival of HTLV-1 infected T cells. Lysine 63 (K63)-linked polyubiquitination of Tax provides an important regulatory mechanism that promotes Tax-mediated interaction with the IKK complex and activation of NF-κB; however, the host proteins regulating Tax ubiquitination are largely unknown. To identify new Tax interacting proteins that may regulate its ubiquitination we conducted a yeast two-hybrid screen using Tax as bait. This screen yielded the E3/E4 ubiquitin conjugation factor UBE4B as a novel binding partner for Tax. Here, we confirmed the interaction between Tax and UBE4B in mammalian cells by co-immunoprecipitation assays and demonstrated colocalization by proximity ligation assay and confocal microscopy. Overexpression of UBE4B specifically enhanced Tax-induced NF-κB activation, whereas knockdown of UBE4B impaired Tax-induced NF-κB activation and the induction of NF-κB target genes in T cells and ATLL cell lines. Furthermore, depletion of UBE4B with shRNA resulted in apoptotic cell death and diminished the proliferation of ATLL cell lines. Finally, overexpression of UBE4B enhanced Tax polyubiquitination, and knockdown or CRISPR/Cas9-mediated knockout of UBE4B attenuated both K48- and K63-linked polyubiquitination of Tax. Collectively, these results implicate UBE4B in HTLV-1 Tax polyubiquitination and downstream NF-κB activation. Author summary: Infection with the retrovirus HTLV-1 leads to the development of either CD4+CD25+ leukemia/lymphoma (ATLL) or a demyelinating neuroinflammatory disease (HAM/TSP) in a subset of infected individuals. The HTLV-1 Tax protein is a regulatory protein which regulates viral gene expression and persistently activates cellular signaling pathways such as NF-κB to drive the clonal expansion and longevity of HTLV-1 infected CD4+ T cells. Polyubiquitination of Tax is a key mechanism of NF-κB activation by assembling and activating IκB kinase (IKK) signaling complexes; however, the host factors regulating Tax ubiquitination have remained elusive. Here, we have identified the E3/E4 ubiquitin conjugation factor UBE4B as a novel Tax binding protein that promotes both K48- and K63-linked polyubiquitination of Tax. Knockdown or knockout of UBE4B impairs Tax-induced NF-κB activation and triggers apoptosis of HTLV-1-transformed cells. Therefore, UBE4B is an integral host factor that supports HTLV-1 Tax polyubiquitination, NF-κB activation and cell survival. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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22. Two-Ply Carbon Nanotube Fiber-Typed Enzymatic Biofuel Cell Implanted in Mice.
- Author
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Lee, Dong Yeop, Yun, Ji-Hyun, Park, Young Bin, Hyeon, Jae Sang, Jang, Yongwoo, Choi, Young-Bong, Kim, Hyug-Han, Kang, Tong Mook, Ovalle, Raquel, Baughman, Ray H., Kim, Sung Min, Kee, Chang Won, and Kim, Seon Jeong
- Abstract
Implantable devices have emerged as a promising industry. It is inevitable that these devices will require a power source to operate in vivo. Thus, to power implantable medical devices, biofuel cells (BFCs) that generate electricity using glucose without an external power supply have been considered. Although implantable BFCs have been developed for application in vivo, they are limited by their bulky electrodes and low power density. In the present study, we attempted to apply to living mice an implantable enzymatic BFC (EBFC) that was previously reported to be a high-power EBFC comprising carbon nanotube yarn electrodes. To improve their mechanical properties and for convenient implantation, the electrodes were coated with Nafion and twisted into a micro-sized, two-ply, one-body system. When the two-ply EBFC system was implanted in the abdominal cavity of mice, it provided a high-power density of 0.3 mW/cm2. The two-ply EBFC system was injected through a needle using a syringe without surgery and the inflammatory response in vivo initially induced by the injection of the EBFC system was attenuated after 7 days, indicating the biocompatibility of the system in vivo. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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23. The electrochemical glucose sensing based on the chitosan-carbon nanotube hybrid.
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Choi, Young-Bong, Kim, Han-Sem, Jeon, Won-Yong, Lee, Bo-Hee, Shin, Ueon Sang, and Kim, Hyug-Han
- Subjects
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GLUCOSE analysis , *CHITOSAN , *CARBON nanotubes , *ELECTROCHEMICAL analysis , *CHARGE exchange , *ELECTRODES - Abstract
Graphical abstract Highlights • The chitosan-carbon nanotube hybrid was prepared by homogenizer easily. • And chitosan-carbon nanotube hybrid was applied for sensing the glucose electrochemically. • The electrochemical performance of glucose was characterized by cyclic voltammetry. Abstract In glucose sensors, the direct electron transfer (DET) method delivers electrons from glucose oxidase (GOx) to an electrode without the intervention of a mediator. Many researchers have developed the DET method using carbon nanotube (CNT)-based composites for the permeation into the enzyme co-factor. We synthesized chitosan (Chit) core-shelled CNTs that were hydrophilic, biocompatible, and easily prepared. Electrodes with three different Chit-CNT weight ratios were prepared, and their morphological and physicochemical properties were characterized by Fourier-transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), x-ray diffraction (XRD), scanning electron microscopy (SEM), and transition electron microscopy (TEM). The Chit-CNT85 composite (85 wt% Chit) performed optimally by cyclic voltammetry (CV), and was covalently modified with GOx via a routine coupling protocol to form the GOx-Chit-CNT85/ITO electrode. This electrode was electrochemically characterized by impedance spectroscopy (EIS). The GOx loading and pH were optimized against the CV redox potential. The electrode exhibited a fast electron-transfer constant (k s) of 8.2 s−1. The cathodic peak of GOx decreased in response to increasing glucose concentrations, but no changes were observed in the presence of physiological interfering substances such as ascorbic acid and uric acid. Therefore, our GOx-Chit-CNT85/ITO electrode has the potential for use in glucose sensing directly without a mediator. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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24. Stretchable Fiber Biofuel Cell by Rewrapping Multiwalled Carbon Nanotube Sheets.
- Author
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Sim, Hyeon Jun, Lee, Dong Yeop, Kim, Hyunsoo, Choi, Young-Bong, Kim, Hyug-Han, Baughman, Ray H., and Kim, Seon Jeong
- Published
- 2018
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25. Peroxisomes support human herpesvirus 8 latency by stabilizing the viral oncogenic protein vFLIP via the MAVS-TRAF complex.
- Author
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Choi, Young Bong, Choi, Yeeun, and Harhaj, Edward William
- Subjects
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PEROXISOMES , *HERPESVIRUSES , *ONCOGENIC proteins , *GENE expression , *CYTOLOGY , *IMMUNOSTAINING - Abstract
Human herpesvirus 8 (HHV-8) is causally related to human malignancies. HHV-8 latent viral FLICE-inhibitory protein (vFLIP) is a viral oncoprotein that is linked to pathogenesis, but how its expression is regulated is largely unknown. In an attempt to understand the role of the mitochondrial antiviral signaling (MAVS) adaptor in HHV-8 infection, we discovered that vFLIP expression was post-translationally up-regulated by the MAVS signaling complex on peroxisomes. Furthermore, we demonstrated that vFLIP could be targeted to the peroxisomes, where it was oncogenically active, in a PEX19-dependent manner. Targeted disruption of vFLIP and MAVS interaction resulted in a decrease in vFLIP expression and selectively promoted death of latently HHV-8-infected cells, providing therapeutic potential for treating HHV-8 diseases. Collectively, our experimental results suggest novel involvement of peroxisomes and MAVS in the stabilization of vFLIP and thereby in the establishment or maintenance of HHV-8 latency and associated pathogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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26. Mediator-free carbon nanotube yarn biofuel cell.
- Author
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Kwon, Cheong Hoon, Park, Young Bin, Lee, Jae Ah, Choi, Young-Bong, Kim, Hyug-Han, Lima, Márcio D., Baughman, Ray H., and Kim, Seon Jeong
- Published
- 2016
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27. HTLV-1 Tax Stabilizes MCL-1 via TRAF6-Dependent K63-Linked Polyubiquitination to Promote Cell Survival and Transformation.
- Author
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Choi, Young Bong and Harhaj, Edward William
- Subjects
- *
HTLV , *SYNTAXINS , *UBIQUITIN , *T cells , *CELL transformation - Abstract
The human T-cell leukemia virus type 1 (HTLV-1) Tax protein hijacks the host ubiquitin machinery to activate IκB kinases (IKKs) and NF-κB and promote cell survival; however, the key ubiquitinated factors downstream of Tax involved in cell transformation are unknown. Using mass spectrometry, we undertook an unbiased proteome-wide quantitative survey of cellular proteins modified by ubiquitin in the presence of Tax or a Tax mutant impaired in IKK activation. Tax induced the ubiquitination of 22 cellular proteins, including the anti-apoptotic BCL-2 family member MCL-1, in an IKK-dependent manner. Tax was found to promote the nondegradative lysine 63 (K63)-linked polyubiquitination of MCL-1 that was dependent on the E3 ubiquitin ligase TRAF6 and the IKK complex. Tax interacted with and activated TRAF6, and triggered its mitochondrial localization, where it conjugated four carboxyl-terminal lysine residues of MCL-1 with K63-linked polyubiquitin chains, which stabilized and protected MCL-1 from genotoxic stress-induced degradation. TRAF6 and MCL-1 played essential roles in the survival of HTLV-1 transformed cells and the immortalization of primary T cells by HTLV-1. Therefore, K63-linked polyubiquitination represents a novel regulatory mechanism controlling MCL-1 stability that has been usurped by a viral oncogene to precipitate cell survival and transformation. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
28. Homogeneous Electrochemical Detection of Hippuric Acid in Urine Based on the Osmium-Antigen Conjugate.
- Author
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Jeon, Won‐Yong, Choi, Young‐Bong, and Kim, Hyug‐Han
- Published
- 2013
- Full Text
- View/download PDF
29. Assurance of mitochondrial integrity and mammalian longevity by the p62-Keap1-Nrf2-Nqo1 cascade.
- Author
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Kwon, Jeongho, Han, Eunhye, Bui, Chi-Bao, Shin, Woochul, Lee, Junho, Lee, Sejeong, Choi, Young-Bong, Lee, Ann-Hwee, Lee, Kyong-Hoon, Park, Chankyu, Obin, Martin S, Park, Sung Kyu, Seo, Yun Jeong, Oh, Goo Taeg, Lee, Han-Woong, and Shin, Jaekyoon
- Abstract
Sqstm1/p62 functions in the non-canonical activation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2). However, its physiological relevance is not certain. Here, we show that p62
−/− mice exhibited an accelerated presentation of ageing phenotypes, and tissues from these mice created a pro-oxidative environment owing to compromised mitochondrial electron transport. Accordingly, mitochondrial function rapidly declined with age in p62−/− mice. In addition, p62 enhanced basal Nrf2 activity, conferring a higher steady-state expression of NAD(P)H dehydrogenase, quinone 1 (Nqo1) to maintain mitochondrial membrane potential and, thereby, restrict excess oxidant generation. Together, the p62-Nrf2-Nqo1 cascade functions to assure mammalian longevity by stabilizing mitochondrial integrity. [ABSTRACT FROM AUTHOR]- Published
- 2012
- Full Text
- View/download PDF
30. Electrogenerated Chemiluminescence Sensor Based on a Self-Assembled Monolayer of Ruthenium(II)-bis(2,2′-bipyridyl)(aminopropyl imidazole) on Gold Deposited Screen Printed Electrode.
- Author
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Kang, Chang Hoon, Choi, Young-Bong, Kim, Hyug-Han, Choi, Han Nim, and Lee, Won-Yong
- Published
- 2011
- Full Text
- View/download PDF
31. Induction of angiogenic chemokine CCL2 by human herpesvirus 8 chemokine receptor
- Author
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Choi, Young Bong and Nicholas, John
- Subjects
- *
NEOVASCULARIZATION , *CHEMOKINES , *LIGANDS (Biochemistry) , *HERPESVIRUSES , *CELL receptors , *VIRAL genetics , *KAPOSI'S sarcoma , *GENETIC transcription - Abstract
Abstract: Human herpesvirus 8 (HHV-8) is associated with Kaposi''s sarcoma (KS), an endothelial cell lesion believed to be initiated and driven primarily by cytokine dysregulation. Among the viral proteins suspected as contributing to viral pathogenesis is the lytically expressed viral G protein-coupled receptor (vGPCR), which can induce various cellular cytokines. CC ligand-2 (CCL2/MCP-1) is a vGPCR-regulated angiogenic chemokine found at elevated levels in KS lesions and induced by HHV-8 infection of endothelial cells. Here we show that vGPCR induces CCL2 in endothelial cells via activation of C/EBPβ and that vGPCR and C/EBPβ are critical components of CCL2 induction by HHV-8 infection of endothelial cultures. To our knowledge, this is the first report of vGPCR-mediated cytokine induction, and its characterization, in the context of virus infection. Our results identify a mechanism by which vGPCR can contribute, in a host cell shutoff-independent manner, to viral pathogenesis. [Copyright &y& Elsevier]
- Published
- 2010
- Full Text
- View/download PDF
32. Microfluidic chip-based electrochemical immunoassay for hippuric acid.
- Author
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Yoo, Sung Ju, Choi, Young Bong, Ju, Jong Il, Tae, Gun-Sik, Kim, Hyug Han, and Lee, Sang-Hoon
- Subjects
- *
MICROFLUIDIC devices , *ELECTROCHEMISTRY , *IMMUNOASSAY , *HIPPURIC acid , *MOLECULAR weights , *TOLUENE , *BIOINDICATORS , *MEDICAL care - Abstract
Urinary hippuric acid (HA), of molecular weight 180 Da, is one of the major metabolites in toluene-exposed humans and is a major biological indicator. Simple and ubiquitous monitoring of exposure to toluene is very important in occupational health care, and a microfluidic chip-based electrochemical immunoassay for rapid and quantitative detection of HA in human urine is proposed in this paper. The system employs a conjugate of ferrocene (Fc) and hippuric acid (HA). The competition between hippuric acid (HA) and the ferrocene-hippuric acid complex (Fc-Lys-HA) to bind with a HA antibody coated onto polybeads generated electrical signals proportional to the HA concentration in the range of 0–40 mg mL−1. All the complicated HA detection processes were integrated on the single microfluidic platform. The quantitative advantages of our HA detection chip are as follows: (1) the total chip size was reduced to 3.0 × 2.0 × 0.5 cm and is small enough to be portable, (2) the assay time took 1 min, and is shorter than that of conventional electrochemical HA immunoassay systems (about 20 min) and (3) 40 μL of the sample solution was enough to detect HA in the range of 0–40 mg mL−1, which is enough range to be used for the point-of-care system. In addition, we suggest the improved chip-based HA assay method by the combination of electrochemical and enzymatic amplification processes for the detection of greater electrical signals. The sensitivity of the combined method was increased about three times compared to that of the non-enzymatic process. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
33. The Transcriptional Corepressor, PELP1, Recruits HDAC2 and Masks Histones Using Two Separate Domains.
- Author
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Choi, Young Bong, Ko, Jin Kyoung, and Shin, Jaekyoon
- Subjects
- *
GLUTAMIC acid , *CHROMATIN , *HISTONES , *AMINO acids , *GENETICS , *TRANSCRIPTION factors , *STEROID hormones , *CELL nuclei , *CELL receptors , *CELL culture , *CELL lines - Abstract
PELP1 (proline-, glutamic acid-, and leucine-rich protein 1) has been recognized as a coactivator of estrogen receptor (ER)-recruiting p300/CREB-binding protein histone acetyltransferase to the target chromosome. The present study shows that PELP1 does indeed coactivate ER-mediated transcription but also serves as a corepressor of other nuclear hormone receptors (NR)- and non-NR sequence-specific transcription factors tested, including GR, Nut77, AP1, NF-κB, and TCF/SRF. PELP1 expression also retarded the proliferation of mouse fibroblast cell lines in which there was no detectable ER. This was due, at least in part, to the suppressed activation of serum-response genes such as c-fos that in turn resulted from the blocked histone hyperacetylation of nucleosomes containing the c-fos promoter region. The N-terminal leucine-abundant region of PELP1 was observed to interact with HDAC2 and exhibited repressive activity when tethered to the chromatin. In addition, the C-terminal glutamic acid-abundant region bound to the hypoacetylated histones H3 and H4 and prevented them from becoming substrates of histone acetyltransferase. Thus PELP1 promotes and maintains the hypoacetylated state of histones at the target genomic site, and ER binding reverses its role to hyperacetylate histones through an as yet unidentified mechanism. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
34. Development of a Glucose Sensor Based on Glucose Dehydrogenase Using Polydopamine-Functionalized Nanotubes.
- Author
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Jeon, Won-Yong, Kim, Hyug-Han, and Choi, Young-Bong
- Subjects
GLUCOSE ,MULTIWALLED carbon nanotubes ,THERMOGRAVIMETRY ,GLUCOSE oxidase ,NANOTUBES ,CARBON electrodes - Abstract
The electrochemical-based detection of glucose is widely used for diagnostic purposes and is mediated by enzyme-mediated signal transduction mechanisms. For such applications, recent attention has focused on utilizing the oxygen-insensitive glucose dehydrogenase (GDH) enzyme in place of the glucose oxidase (GOx) enzyme, which is sensitive to oxygen levels. Currently used Ru-based redox mediators mainly work with GOx, while Ru(dmo–bpy)
2 Cl2 has been proposed as a promising mediator that works with GDH. However, there remains an outstanding need to improve Ru(dmo–bpy)2 Cl2 attachment to electrode surfaces. Herein, we report the use of polydopamine-functionalized multi-walled carbon nanotubes (PDA-MWCNTs) to effectively attach Ru(dmo–bpy)2 Cl2 and GDH onto screen-printed carbon electrodes (SPCEs) without requiring a cross–linker. PDA-MWCNTs were characterized by Fourier transform infrared (FT–IR) spectroscopy, Raman spectroscopy, and thermal gravimetric analysis (TGA), while the fabrication and optimization of Ru(dmo–bpy)2 Cl2 /PDA-MWCNT/SPCEs were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) measurements. The experimental results demonstrate a wide linear range of glucose-concentration-dependent responses and the multi-potential step (MPS) technique facilitated the selective detection of glucose in the presence of physiologically relevant interfering species, as well as in biological fluids (e.g., serum). The ease of device fabrication and high detection performance demonstrate a viable pathway to develop glucose sensors based on the GDH enzyme and Ru(dmo–bpy)2 Cl2 redox mediator and the sensing strategy is potentially extendable to other bioanalytes as well. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
35. Herpesvirus Regulation of Selective Autophagy.
- Author
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Vo, Mai Tram, Choi, Young Bong, and Weidner-Glunde, Magdalena
- Subjects
- *
AUTOPHAGY , *HERPESVIRUSES , *INTRACELLULAR pathogens , *QUALITY control , *ORGANELLES - Abstract
Selective autophagy has emerged as a key mechanism of quality and quantity control responsible for the autophagic degradation of specific subcellular organelles and materials. In addition, a specific type of selective autophagy (xenophagy) is also activated as a line of defense against invading intracellular pathogens, such as viruses. However, viruses have evolved strategies to counteract the host's antiviral defense and even to activate some proviral types of selective autophagy, such as mitophagy, for their successful infection and replication. This review discusses the current knowledge on the regulation of selective autophagy by human herpesviruses. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
36. Pentacyanoammineferrate-Based Non-Enzymatic Electrochemical Biosensing Platform for Selective Uric Acid Measurement.
- Author
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Jeon, Won-Yong, Lee, Chang-Jun, Sut, Tun Naw, Kim, Hyug-Han, Choi, Young-Bong, and Rusling, James F.
- Subjects
ELECTRODE performance ,GLUCOSE analysis ,INDIUM tin oxide ,DOPAMINE ,VITAMIN C ,IMPEDANCE spectroscopy ,CYCLIC voltammetry ,URIC acid - Abstract
The electrochemical-based detection of uric acid (UA) is widely used for diagnostic purposes. However, various interfering species such as ascorbic acid, dopamine, and glucose can affect electrochemical signals, and hence there is an outstanding need to develop improved sensing platforms to detect UA with high selectivity. Herein, we report a pentagonal mediator-based non-enzymatic electrochemical biosensing platform to selectively measure UA in the presence of interfering species. The working electrode was fabricated by electrodepositing polymerized 1-vinylimidazole (PVI), which has an imidazole ligand, onto indium tin oxide (ITO), and then conjugating nickel ions to the PVI-coated ITO electrode. Electrode performance was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) measurements and integrated together with pentacyanoammineferrate, which can bind to the amine groups of UA and function as an electron transferring mediator. The experimental results showed a wide linear range of UA concentration-dependent responses and the multi-potential step (MPS) technique facilitated selective detection of UA in the presence of physiologically relevant interfering species. Altogether, these findings support that pentacyanoammineferrate-based non-enzymatic electrodes are suitable biosensing platforms for the selective measurement of UA, and such approaches could potentially be extended to other bioanalytes as well. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
37. High-power biofuel cell textiles from woven biscrolled carbon nanotube yarns.
- Author
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Kwon, Cheong Hoon, Lee, Sung-Ho, Choi, Young-Bong, Lee, Jae Ah, Kim, Shi Hyeong, Kim, Hyug-Han, Spinks, Geoffrey M., Wallace, Gordon G., Lima, Márcio D., Kozlov, Mikhail E., Baughman, Ray H., and Kim, Seon Jeong
- Published
- 2014
- Full Text
- View/download PDF
38. The Itch ubiquitin ligase is required for KSHV RTA induced vFLIP degradation.
- Author
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Chmura, Jennifer C., Herold, Kevin, Ruffin, Ayana, Atuobi, Trudymae, Fabiyi, Yetunde, Mitchell, Ashley E., Choi, Young Bong, and Ehrlich, Elana S.
- Subjects
- *
KAPOSI'S sarcoma-associated herpesvirus diseases , *UBIQUITIN ligases , *NF-kappa B , *GENE expression in viruses , *PROTEASOMES , *VIRUS reactivation - Abstract
Expression of Kaposi's sarcoma herpesvirus vFLIP, a potent activator of NFkB signaling, promotes latency. Inhibition of NFkB signaling promotes lytic reactivation. We previously reported that lytic inducer, RTA, inhibits vFLIP induced NFkB signaling by inducing the degradation of vFLIP via the proteasome. Here we report that the cellular ubiquitin ligase, Itch, is required for RTA induced degradation of vFLIP. Expression of either Itch targeting shRNA or a dominant negative mutant of the ubiquitin ligase both increased the stability of vFLIP in the presence of RTA. Itch potently ubiquitinated vFLIP in vivo and in vitro . We provide evidence for interaction between RTA, vFLIP and Itch and we identified an RTA resistant mutant of vFLIP that is unable to interact with Itch. These observations contribute to our understanding of how RTA counteracts the activities of vFLIP. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
39. A stable glucose sensor with direct electron transfer, based on glucose dehydrogenase and chitosan hydro bonded multi-walled carbon nanotubes.
- Author
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Jeon, Won-Yong, Kim, Han-Sem, Jang, Hye-Won, Lee, Ye-Sung, Shin, Ueon Sang, Kim, Hyug-Han, and Choi, Young-Bong
- Subjects
- *
MULTIWALLED carbon nanotubes , *GLUCOSE analysis , *CHARGE exchange , *FLAVIN adenine dinucleotide , *VAN der Waals forces , *GLUCOSE , *CHITOSAN - Abstract
Direct electron transfer (DET) glucose sensors are third-generation biosensors, which allow the enzyme to directly transfer electrons by glucose oxidation without a mediator. In previous studies, many researchers have focused on the glucose oxidase (GOx)-based DET glucose sensor; however, an efficient DET glucose sensor using flavin adenine dinucleotide (FAD)-glucose dehydrogenase (GDH) has not been developed. In the present study, we bound FAD-GDH and multi-walled carbon nanotubes (MWCNT), using chitosan (CS) compounds that support hydrogen bonding, van der Waals forces, and 3D structural adsorption. The GDH/CS-MWCNT-5 composite, which had a GDH concentration of 75 wt%, was physically adsorbed on screen-printed carbon electrodes (SPCEs), and cyclic voltammetry indicated that its oxidation and reduction peaks were at − 0.422 V and − 0.543 V (vs Ag/AgCl), respectively. In addition, the electrochemical results confirmed that the prepared GDH/CS-MWCNT/SPCEs were not affected by other interfering substances or oxygen at pH of 7. The GDH/CS-MWCNT/SPCEs displayed oxidation catalytic currents, which increased according to glucose concentrations across a range of 0–5.5 mM. Finally, the short-term stability of glucose, assessed for 10 days, was maintained at 80% of the GDH enzyme activity for 6 days, and it reduced to 50% of the initial activity for the remaining 4 days. Here, we illustrate the potential utility of the FAD-GDH-based DET method in continuous glucose monitoring sensors. Diagram of the GDH/CS-MWCNT fabrication process and glucose sensing [Display omitted] • CS-MWCNTs were conjugated with FAD-GDH by hydrogen bonding. • CS-MWCNTs were optimized with GDH for DET glucose sensing. • GDH/CS-MWCNT showed good response with glucose under interfering substances. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
40. Transcriptional and Post-transcriptional Regulation of the PKCδ Gene by Etoposide in L1210 Murine Leukemia Cells: Implication of PKCδ Autoregulation
- Author
-
Shin, Soon Young, Kim, Chang Gun, Ko, Jesang, Min, Do Sik, Chang, Jong-Soo, Ohba, Motoi, Kuroki, Toshio, Choi, Young Bong, Kim, Young-Ho, Na, Doe Sun, Kim, Jin Woo, and Lee, Young Han
- Subjects
- *
ETOPOSIDE , *GLUCOSIDES , *PROTEIN kinases , *INFECTION - Abstract
Protein kinase C δ (PKCδ) plays an important role in the regulation of apoptosis in response to diverse anticancer agents. PKCδ is cleaved irreversibly to a catalytically active fragment in response to apoptotic stimuli; however, little information is available about the regulation of PKCδ gene expression. In this study, we found that the amount of steady-state PKCδ mRNA and protein was increased by etoposide in mouse L1210 leukemia cells. The transcriptional rate of the PKCδ gene and the stability of PKCδ mRNA were increased by treatment with etoposide, resulting in the accumulation of PKCδ protein. Rottlerin inhibited etoposide-induced PKCδ gene expression significantly, while Go6976, LY294002 and PD98059 had no effect. Further, both stable and adenovirus-mediated expression of a dominant negative PKCδ(KR) abrogated etoposide-induced PKCδ expression. Etoposide-stimulated PKCδ transcription but not PKCδ mRNA stability was blocked completely by pretreatment with rottlerin. Our data reveal a novel mechanism whereby PKCδ gene is regulated at the transcriptional and post-transcriptional level in the L1210 leukemia cell line. [Copyright &y& Elsevier]
- Published
- 2004
- Full Text
- View/download PDF
41. Carbon nanotubes immobilized on gold electrode as an electrochemical humidity sensor.
- Author
-
Kim, Han-Sem, Kim, Ju Hyeon, Park, Sang-Yu, Kang, Ji-Hye, Kim, Sung-Jin, Choi, Young-Bong, and Shin, Ueon Sang
- Subjects
- *
GOLD electrodes , *ELECTROCHEMICAL electrodes , *CARBON nanotubes , *ELECTROCHEMICAL sensors , *TRANSMISSION electron microscopy - Abstract
(A) Schematic illustration and SEM and TEM images of CS-shell/MWCNT-core nanohybrids structure. (B) Photograph of commercial alumina-based humidity sensor electrode. (C) Photograph of the humidity sensor electrode coated by CS-MWCNT nanohybrids and SEM image showing electrodes interconnected by CS-MWCNT nanohybrids. • Fabrication of chitosan-MWCNT core–shell-structured nanohybrid (CS-MWCNT) on a gold electrode as an electrochemical humidity sensor. • CS-MWCNT nanohybrid fibers exhibiting low resistance without moisture. • Remarkable results in the humidity response with an efficient humidity sensing range of 30–90%. Multi-walled carbon nanotubes (MWCNTs) were fabricated on a gold electrode to develop a resistance-sensing humidity sensor with low resistance. Core-shell-structured nanohybrids were prepared using Chitosan-MWCNT to obtain good hydrophilicity, resulting in a highly responsive humidity sensor. We also measured resistance, which changes due to hydrogen bonding between the amine group of chitosan and H 2 O. The structure of the fabricated chitosan-MWCNT core–shell-structured nanohybrid was measured. Furthermore, we determined the highest response and linearity of the CS-MWCNT25 nanohybrids based on the number of coats on the electrode and the composition ratio of chitosan and MWCNTs. The fabricated CS-MWCNT25 nanohybrid humidity sensor exhibited good electrical efficiency without voltage or frequency dependence and a low-temperature dependency with a temperature coefficient (RH/°C) of 0.187%. Finally, a fast recovery time of 40 s between 30% and 100% RH, a maximum humidity hysteresis of less than 0.300 ± 0.001%, and long-term stability for 2 months indicated that this is an excellent humidity sensor. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
42. Herpesvirus lytic infection-induced mitophagy via viral interferon regulatory factor 1.
- Author
-
Vo MT and Choi YB
- Abstract
Viral control of mitochondria via mitophagy has a dampening effect on mitochondrion-mediated innate immune responses. We previously found that human herpesvirus 8 (HHV-8) could activate mitophagy via its lytic gene product vIRF-1 (viral interferon regulatory factor 1). Mechanistically, we previously demonstrated that vIRF-1 interacts with the mitophagic proteins BNIP3L (BCL2 interacting protein 3 like) and TUFM (Tu translation elongation factor, mitochondrial). Despite these significant findings, however, the precise molecular mechanisms underlying vIRF-1-activated mitophagy, particularly with core components of the autophagy machinery, remained to be fully elucidated. We recently reported that vIRF-1 binds preferentially and directly to GABARAPL1 (GABA type A receptor associated protein like 1) in a noncanonical manner, and this interaction is essential for virus-productive replication. Furthermore, we found that BNIP3L is a crucial factor that promotes vIRF-1 oligomerization and associated mitophagy activation, including GABARAPL1 interaction with vIRF-1 and TUFM dimerization. Together, our findings deepen our understanding of lytic infection-induced mitophagy and provide the key protein-protein interactions involved in vIRF-1-mediated mitophagy., Competing Interests: Disclosure statement All authors declare no conflict of interest.
- Published
- 2023
- Full Text
- View/download PDF
43. Novel Functions and Virus-Host Interactions Implicated in Pathogenesis and Replication of Human Herpesvirus 8.
- Author
-
Choi YB, Cousins E, and Nicholas J
- Subjects
- Animals, Humans, Castleman Disease virology, Herpesvirus 8, Human genetics, MicroRNAs, Sarcoma, Kaposi virology
- Abstract
Human herpesvirus 8 (HHV-8) is classified as a γ2-herpesvirus and is related to Epstein-Barr virus (EBV), a γ1-herpesvirus. One important aspect of the γ-herpesviruses is their association with neoplasia, either naturally or in animal model systems. HHV-8 is associated with B-cell-derived primary effusion lymphoma (PEL) and multicentric Castleman's disease (MCD), endothelial-derived Kaposi's sarcoma (KS), and KSHV inflammatory cytokine syndrome (KICS). EBV is also associated with a number of B-cell malignancies, such as Burkitt's lymphoma, Hodgkin's lymphoma, and posttransplant lymphoproliferative disease, in addition to epithelial nasopharyngeal and gastric carcinomas. Despite the similarities between these viruses and their associated malignancies, the particular protein functions and activities involved in key aspects of virus biology and neoplastic transformation appear to be quite distinct. Indeed, HHV-8 specifies a number of proteins for which counterparts had not previously been identified in EBV, other herpesviruses, or even viruses in general, and these proteins are believed to play vital functions in virus biology and to be involved centrally in viral pathogenesis. Additionally, a set of microRNAs encoded by HHV-8 appears to modulate the expression of multiple host proteins to provide conditions conductive to virus persistence within the host and possibly contributing to HHV-8-induced neoplasia. Here, we review the molecular biology underlying these novel virus-host interactions and their potential roles in both virus biology and virus-associated disease.
- Published
- 2021
- Full Text
- View/download PDF
44. Human Herpesvirus 8 Interferon Regulatory Factors 1 and 3 Mediate Replication and Latency Activities via Interactions with USP7 Deubiquitinase.
- Author
-
Xiang Q, Ju H, Li Q, Mei SC, Chen D, Choi YB, and Nicholas J
- Subjects
- Amino Acid Motifs, Cell Line, Tumor, HEK293 Cells, Herpesviridae Infections genetics, Herpesviridae Infections pathology, Humans, Interferon Regulatory Factors genetics, Ubiquitin-Specific Peptidase 7 genetics, Viral Proteins genetics, Gene Expression Regulation, Viral physiology, Herpesviridae Infections metabolism, Herpesvirus 8, Human physiology, Interferon Regulatory Factors metabolism, Ubiquitin-Specific Peptidase 7 metabolism, Viral Proteins metabolism, Virus Latency physiology
- Abstract
Human herpesvirus 8 (HHV-8) encodes four viral interferon regulatory factors (vIRF-1 to -4) that likely function to suppress innate immune and cellular stress responses through inhibitory interactions with various cellular proteins involved in these activities. It is notable that vIRF-1 and -4 have been reported to interact with the deubiquitinase ubiquitin-specific protease 7 (USP7), substrates of which include p53 and the p53-targeting and -destabilizing ubiquitin E3 ligase MDM2. Structural studies of vIRF-1 and vIRF-4 USP7 binding sequences in association with USP7 have been reported; both involve interactions with N-terminal-domain residues of USP7 via EGPS and ASTS motifs in vIRF-1 and vIRF-4, respectively, but vIRF-4 residues also contact the catalytic site. However, the biological activities of vIRF-1 and vIRF-4 via USP7 interactions are unknown. Here, we report that vIRF-3, which is latently, as well as lytically, expressed in HHV-8-infected primary effusion lymphoma (PEL) cells, also interacts with USP7-via duplicated EGPS motifs-and that this interaction is important for PEL cell growth and viability. The interaction also contributes to suppression of productive virus replication by vIRF-3, which we identify here. We further show that vIRF-1, which is expressed at low levels in PEL latency, promotes latent PEL cell viability and that this activity and vIRF-1-promoted productive replication (reported previously) involve EGPS motif-mediated USP7 targeting by vIRF-1. This study is the first to identify latent and lytic functions of vIRF-1 and vIRF-3, respectively, and to address the biological activities of these vIRFs through their interactions with USP7. IMPORTANCE HHV-8 is associated with Kaposi's sarcoma, primary effusion lymphoma (PEL), and multicentric Castleman's disease; both latent and lytic viral functions are believed to contribute. Viral interferon regulatory factors specified by HHV-8 are thought to be critically important for successful productive replication through suppression of innate immune and stress responses triggered by the lytic cycle. Latently expressed vIRF-3 contributes significantly to PEL cell survival. Here, we identify ubiquitin-specific protease 7 (USP7) deubiquitinase targeting by vIRF-3 (in addition to previously reported USP7 binding by vIRF-1 and vIRF-4); the importance of vIRF-1 and vIRF-3 interactions with USP7 for latent PEL cell growth and viability; and the positive and negative contributions, respectively, of USP7 targeting by vIRF-1 and vIRF-3 to HHV-8 productive replication. This is the first report of the biological importance of vIRF-1 in PEL cell latency, the modulation of productive replication by vIRF-3, and the contributions of vIRF-USP7 interactions to HHV-8 biology., (Copyright © 2018 American Society for Microbiology.)
- Published
- 2018
- Full Text
- View/download PDF
45. A Simple Interfacial Platform for Homogeneous Electrochemical Immunoassays Using a Poly(Vinylimidazole)-Modified Electrode.
- Author
-
Choi YB, Jeon WY, and Kim HH
- Subjects
- Electrochemical Techniques methods, Electrodes, Hippurates chemistry, Immunoassay methods, Oxidation-Reduction, Photoelectron Spectroscopy methods, Biosensing Techniques methods
- Abstract
In this study, a homogeneous method featuring simple, one-step detection was developed to analyze hippuric acid (HA), a major metabolite of toluene. High sensitivity was achieved with the facile immobilization of poly(vinylimidazole) (PVI) on an indium tin oxide (ITO) electrode. Using a previously developed approach, pentacyanoferrate was coordinated with pyridyl- N ligands, and the redox-active Fe(II/III) centers were bound to Ni(II) ions on the electrode via electrostatic cyanide bridges. The detection was accomplished by the competitive binding of free HA and pentacyanoferrate-(4-aminomethylpyridine-hippuric acid) (Fe-HA, the electron transfer mediator) to the HA antibody on the Ni(II) ions-modified PVI-ITO (Ni-PVI-ITO) electrode. The electrical and physicochemical characterization of the electrode was carried out by cyclic voltammetry, differential pulse voltammetry, field emission scanning electron microscopy, and X-ray photoelectron spectroscopy. At low mediator concentrations, the electrical signals were proportional to the HA concentration between 0.1 µg/mL and 1.0 mg/mL. The same method may be extended to other small organic molecules., Competing Interests: The authors declare no conflict of interest.
- Published
- 2016
- Full Text
- View/download PDF
46. TAX1BP1 Restrains Virus-Induced Apoptosis by Facilitating Itch-Mediated Degradation of the Mitochondrial Adaptor MAVS.
- Author
-
Choi YB, Shembade N, Parvatiyar K, Balachandran S, and Harhaj EW
- Subjects
- Animals, Apoptosis, HEK293 Cells, HeLa Cells, Humans, Mice, Sendai virus pathogenicity, Ubiquitination, Vesiculovirus pathogenicity, Adaptor Proteins, Signal Transducing metabolism, Intracellular Signaling Peptides and Proteins metabolism, Mitochondria metabolism, Neoplasm Proteins metabolism, RNA Viruses pathogenicity, Repressor Proteins metabolism, Ubiquitin-Protein Ligases metabolism
- Abstract
The host response to RNA virus infection consists of an intrinsic innate immune response and the induction of apoptosis as mechanisms to restrict viral replication. The mitochondrial adaptor molecule MAVS plays critical roles in coordinating both virus-induced type I interferon production and apoptosis; however, the regulation of MAVS-mediated apoptosis is poorly understood. Here, we show that the adaptor protein TAX1BP1 functions as a negative regulator of virus-induced apoptosis. TAX1BP1-deficient cells are highly sensitive to apoptosis in response to infection with the RNA viruses vesicular stomatitis virus and Sendai virus and to transfection with poly(I·C). TAX1BP1 undergoes degradation during RNA virus infection, and loss of TAX1BP1 is associated with apoptotic cell death. TAX1BP1 deficiency augments virus-induced activation of proapoptotic c-Jun N-terminal kinase (JNK) signaling. Virus infection promotes the mitochondrial localization of TAX1BP1 and concomitant interaction with the mitochondrial adaptor MAVS. TAX1BP1 recruits the E3 ligase Itch to MAVS to trigger its ubiquitination and degradation, and loss of TAX1BP1 or Itch results in increased MAVS protein expression. Together, these results indicate that TAX1BP1 functions as an adaptor molecule for Itch to target MAVS during RNA virus infection and thus restrict virus-induced apoptosis., (Copyright © 2016 American Society for Microbiology.)
- Published
- 2016
- Full Text
- View/download PDF
47. Disposable Non-Enzymatic Glucose Sensors Using Screen-Printed Nickel/Carbon Composites on Indium Tin Oxide Electrodes.
- Author
-
Jeon WY, Choi YB, and Kim HH
- Subjects
- Biosensing Techniques methods, Carbon chemistry, Electrochemical Techniques methods, Electrodes, Surface Properties, Biosensing Techniques instrumentation, Electrochemical Techniques instrumentation, Glucose analysis, Nickel chemistry, Tin Compounds chemistry
- Abstract
Disposable screen-printed nickel/carbon composites on indium tin oxide (ITO) electrodes (DSPNCE) were developed for the detection of glucose without enzymes. The DSPNCE were prepared by screen-printing the ITO substrate with a 50 wt% nickel/carbon composite, followed by curing at 400 °C for 30 min. The redox couple of Ni(OH)₂/NiOOH was deposited on the surface of the electrodes via cyclic voltammetry (CV), scanning from 0-1.5 V for 30 cycles in 0.1 M NaOH solution. The DSPNCE were characterized by field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and electrochemical methods. The resulting electrical currents, measured by CV and chronoamperometry at 0.65 V vs. Ag/AgCl, showed a good linear response with glucose concentrations from 1.0-10 mM. Also, the prepared electrodes showed no interference from common physiologic interferents such as uric acid (UA) or ascorbic acid (AA). Therefore, this approach allowed the development of a simple, disposable glucose biosensor.
- Published
- 2015
- Full Text
- View/download PDF
48. Modulation of Mitochondrial Antiviral Signaling by Human Herpesvirus 8 Interferon Regulatory Factor 1.
- Author
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Hwang KY and Choi YB
- Subjects
- Cell Line, Humans, Adaptor Proteins, Signal Transducing metabolism, Herpesvirus 8, Human immunology, Herpesvirus 8, Human physiology, Host-Pathogen Interactions, Immune Evasion, Interferon Regulatory Factors metabolism, Signal Transduction, Viral Proteins metabolism
- Abstract
Unlabelled: Mitochondrial lipid raft-like microdomains, experimentally also termed mitochondrial detergent-resistant membrane fractions (mDRM), play a role as platforms for recruiting signaling molecules involved in antiviral responses such as apoptosis and innate immunity. Viruses can modulate mitochondrial functions for their own survival and replication. However, viral regulation of the antiviral responses via mDRM remains incompletely understood. Here, we report that human herpesvirus 8 (HHV-8) gene product viral interferon regulatory factor 1 (vIRF-1) is targeted to mDRM during virus replication and negatively regulates the mitochondrial antiviral signaling protein (MAVS)-mediated antiviral responses. The N-terminal region of vIRF-1 interacts directly with membrane lipids, including cardiolipin. In addition, a GxRP motif within the N terminus of vIRF-1, conserved in the mDRM-targeting region of mitochondrial proteins, including PTEN-induced putative kinase 1 (PINK1) and MAVS, was found to be important for vIRF-1 association with mitochondria. Furthermore, MAVS, which has the potential to promote vIRF-1 targeting to mDRM possibly by inducing cardiolipin exposure on the outer membrane of mitochondria, interacts with vIRF-1, which, in turn, inhibits MAVS-mediated antiviral signaling. Consistent with these results, vIRF-1 targeting to mDRM contributes to promotion of HHV-8 productive replication and inhibition of associated apoptosis. Combined, our results suggest novel molecular mechanisms for negative-feedback regulation of MAVS by vIRF-1 during virus replication., Importance: Successful virus replication is in large part achieved by the ability of viruses to counteract apoptosis and innate immune responses elicited by infection of host cells. Recently, mitochondria have emerged to play a central role in antiviral signaling. In particular, mitochondrial lipid raft-like microdomains appear to function as platforms in cell apoptosis signaling. However, viral regulation of antiviral signaling through the mitochondrial microdomains remains incompletely understood. The present study demonstrates that HHV-8-encoded vIRF-1 targets to the mitochondrial detergent-resistant microdomains via direct interaction with cardiolipin and inhibits MAVS protein-mediated apoptosis and type I interferon gene expression in a negative-feedback manner, thus promoting HHV-8 productive replication. These results suggest that vIRF-1 is the first example of a viral protein to inhibit mitochondrial antiviral signaling through lipid raft-like microdomains., (Copyright © 2015, American Society for Microbiology. All Rights Reserved.)
- Published
- 2015
- Full Text
- View/download PDF
49. Heterogeneous electrochemical immunoassay of hippuric acid on the electrodeposited organic films.
- Author
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Choi YB, Kim NH, Kim SH, Tae GS, and Kim HH
- Subjects
- Antibodies chemistry, Antibodies immunology, Antigens immunology, Antigens isolation & purification, Hippurates chemistry, Hippurates immunology, Humans, Limit of Detection, Nickel chemistry, Toluene chemistry, Biosensing Techniques methods, Electrochemical Techniques methods, Hippurates isolation & purification, Immunoassay methods, Iron chemistry
- Abstract
By directly coordinating hippuric acid (HA) to the ferrate (Fe) as an electron transfer mediator, we synthesized a Fe-HA complex, which shows a good electrochemical signal and thus enables the electrochemical immunoanalysis for HA. We electrodeposited organic films containing imidazole groups on the electrode surface and then bonded Ni ion (positive charge) to induce immobilization of Fe-HA (negative charge) through the electrostatic interaction. The heterogeneous competitive immunoassay system relies on the interaction between immobilized Fe-HA antigen conjugate and free HA antigen to its antibody (anti-HA). The electric signal becomes weaker due to the hindered electron transfer reaction when a large-sized HA antibody is bound onto the Fe-HA. However, in the presence of HA, the electric signal increases because free HA competitively reacts with the HA antibody prior to actual reaction and thus prevents the HA antibody from interacting with Fe-HA at the electrode surface. This competition reaction enabled an electrochemical quantitative analysis of HA concentration with a detection limit of 0.5 μg mL(-1), and thus allowed us to develop a simple and rapid electrochemical immunosensor.
- Published
- 2014
- Full Text
- View/download PDF
50. Human herpesvirus 8 interferon regulatory factor-mediated BH3-only protein inhibition via Bid BH3-B mimicry.
- Author
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Choi YB, Sandford G, and Nicholas J
- Subjects
- Amino Acid Sequence, Apoptosis physiology, Apoptosis Regulatory Proteins chemistry, Apoptosis Regulatory Proteins metabolism, BH3 Interacting Domain Death Agonist Protein chemistry, BH3 Interacting Domain Death Agonist Protein metabolism, Bcl-2-Like Protein 11, Cell Line, Fluorescent Antibody Technique, Humans, Immunoblotting, Immunoprecipitation, In Situ Nick-End Labeling, Membrane Proteins chemistry, Membrane Proteins metabolism, Molecular Sequence Data, Protein Structure, Secondary, Proto-Oncogene Proteins chemistry, Proto-Oncogene Proteins metabolism, Transfection, Herpesviridae Infections metabolism, Herpesvirus 8, Human metabolism, Immune Evasion, Interferon Regulatory Factors metabolism, Molecular Mimicry, Viral Proteins metabolism
- Abstract
Viral replication efficiency is in large part governed by the ability of viruses to counteract pro-apoptotic signals induced by infection of host cells. For HHV-8, viral interferon regulatory factor-1 (vIRF-1) contributes to this process in part via inhibitory interactions with BH3-only protein (BOP) Bim, recently identified as an interaction partner of vIRF-1. Here we recognize that the Bim-binding domain (BBD) of vIRF-1 resembles a region (BH3-B) of Bid, another BOP, which interacts intramolecularly with the functional BH3 domain of Bid to inhibit it pro-apoptotic activity. Indeed, vIRF-1 was found to target Bid in addition to Bim and to interact, via its BBD region, with the BH3 domain of each. In functional assays, BBD could substitute for BH3-B in the context of Bid, to suppress Bid-induced apoptosis in a BH3-binding-dependent manner, and vIRF-1 was able to protect transfected cells from apoptosis induced by Bid. While vIRF-1 can mediate nuclear sequestration of Bim, this was not the case for Bid, and inhibition of Bid and Bim by vIRF-1 could occur independently of nuclear localization of the viral protein. Consistent with this finding, direct BBD-dependent inactivation by vIRF-1 of Bid-induced mitochondrial permeabilization was demonstrable in vitro and isolated BBD sequences were also active in this assay. In addition to Bim and Bid BH3 domains, BH3s of BOPs Bik, Bmf, Hrk, and Noxa also were found to bind BBD, while those of both pro- and anti-apoptotic multi-BH domain Bcl-2 proteins were not. Finally, the significance of Bid to virus replication was demonstrated via Bid-depletion in HHV-8 infected cells, which enhanced virus production. Together, our data demonstrate and characterize BH3 targeting and associated inhibition of BOP pro-apoptotic activity by vIRF-1 via Bid BH3-B mimicry, identifying a novel mechanism of viral evasion from host cell defenses.
- Published
- 2012
- Full Text
- View/download PDF
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